| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
|
Data corrente: |
11/12/2019 |
|
Data da última atualização: |
11/12/2019 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
PIZAURO, L. J. L.; ALMEIDA, C. C. de; SOLTES, G. A.; SLAVIC, D.; ÁVILA, F. A. de; ZAFALON, L. F.; MACINNES, J. I. |
|
Afiliação: |
Lucas J. L. Pizauro, UNESP; Camila C. de Almeida, UNESP; Glenn A. Soltes, University of Guelph; Durda Slavic, University of Guelph; Fernando A. de Ávila, UNESP; LUIZ FRANCISCO ZAFALON, CPPSE; Janet I. MacInnes, University of Guelph. |
|
Título: |
Detection of antibiotic resistance, mecA, and virulence genes in coagulase-negative Staphylococcus spp. from buffalo milk and the milking environment. |
|
Ano de publicação: |
2019 |
|
Fonte/Imprenta: |
Journal of Dairy Science, v.102, n.12, dec. 2019. |
|
Páginas: |
p.11459-11464 |
|
DOI: |
https://doi.org/10.3168/jds.2018-15920 |
|
Idioma: |
Inglês |
|
Notas: |
Short communication. |
|
Conteúdo: |
The aim of this study was to determinate whether coagulase-negative staphylococci (CNS) from buffalo milk or the milking environment possess virulence factors that are associated with intramammary infections or antimicrobial resistance. Milk samples (n = 320) from 80 lactating buffalo were evaluated for clinical and subclinical mastitis by physical examination, the strip cup test, California Mastitis Test (CMT), and somatic cell count (SCC) over a 4-mo period. In addition, swabs were obtained from the hands of consenting milkers (16), liners (64), and from the mouths (15) and nostrils (15) of buffalo calves. No clinical cases of mastitis were observed; however, CMT together with SCC results indicated that 8 animals had subclinical mastitis. Eighty-four CNS isolates were identified by MALDI-TOF MS and cydB real-time PCR (qPCR) and then evaluated by qPCR for presence of the eta, etb, sea, sec, cna, seb, sei, seq, sem, seg, see, and tst toxin genes, adhesion- and biofilm-associated genes (eno, ebps, fib, fnbA, coa), and the methicillin resistance gene (mecA). Resistance to antibiotics commonly used for mastitis treatment in Brazil was determined using the Kirby-Bauer test. Two strains were positive for the see and eta toxin genes; and mecA (1), eno (27), ebps (10), fnbA (10), and coa (5) genes were also detected. A notable number of isolates were resistant to erythromycin (30), penicillin (26), and cotrimoxazole (18); importantly, 10 vancomycin-resistant isolates were also detected. A smaller number of isolates were resistant to rifampicin (8), oxacillin (7), clindamycin (5), cefepime (4), tetracycline (3), ciprofloxacin (2), and chloramphenicol (1), and none were resistant to gentamicin or ciprofloxacin. Isolates with resistance to 2 (13 isolates), 3 (3), 4 (3), 5 (1), and 6 (1) antibiotics were detected. Taken together, our findings suggest that CNS isolates may not be a significant cause of clinical or even subclinical mastitis in buffaloes, but they may be a reservoir of virulence and antibiotic resistance genes. MenosThe aim of this study was to determinate whether coagulase-negative staphylococci (CNS) from buffalo milk or the milking environment possess virulence factors that are associated with intramammary infections or antimicrobial resistance. Milk samples (n = 320) from 80 lactating buffalo were evaluated for clinical and subclinical mastitis by physical examination, the strip cup test, California Mastitis Test (CMT), and somatic cell count (SCC) over a 4-mo period. In addition, swabs were obtained from the hands of consenting milkers (16), liners (64), and from the mouths (15) and nostrils (15) of buffalo calves. No clinical cases of mastitis were observed; however, CMT together with SCC results indicated that 8 animals had subclinical mastitis. Eighty-four CNS isolates were identified by MALDI-TOF MS and cydB real-time PCR (qPCR) and then evaluated by qPCR for presence of the eta, etb, sea, sec, cna, seb, sei, seq, sem, seg, see, and tst toxin genes, adhesion- and biofilm-associated genes (eno, ebps, fib, fnbA, coa), and the methicillin resistance gene (mecA). Resistance to antibiotics commonly used for mastitis treatment in Brazil was determined using the Kirby-Bauer test. Two strains were positive for the see and eta toxin genes; and mecA (1), eno (27), ebps (10), fnbA (10), and coa (5) genes were also detected. A notable number of isolates were resistant to erythromycin (30), penicillin (26), and cotrimoxazole (18); importantly, 10 vancomycin-resistant isolates were also dete... Mostrar Tudo |
|
Palavras-Chave: |
Dairy buffalo; Virulence genes. |
|
Thesaurus Nal: |
Antibiotic resistance; Biofilm. |
|
Categoria do assunto: |
H Saúde e Patologia |
|
Marc: |
LEADER 02937naa a2200277 a 4500
001 2116604
005 2019-12-11
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3168/jds.2018-15920$2DOI
100 1 $aPIZAURO, L. J. L.
245 $aDetection of antibiotic resistance, mecA, and virulence genes in coagulase-negative Staphylococcus spp. from buffalo milk and the milking environment.$h[electronic resource]
260 $c2019
300 $ap.11459-11464
500 $aShort communication.
520 $aThe aim of this study was to determinate whether coagulase-negative staphylococci (CNS) from buffalo milk or the milking environment possess virulence factors that are associated with intramammary infections or antimicrobial resistance. Milk samples (n = 320) from 80 lactating buffalo were evaluated for clinical and subclinical mastitis by physical examination, the strip cup test, California Mastitis Test (CMT), and somatic cell count (SCC) over a 4-mo period. In addition, swabs were obtained from the hands of consenting milkers (16), liners (64), and from the mouths (15) and nostrils (15) of buffalo calves. No clinical cases of mastitis were observed; however, CMT together with SCC results indicated that 8 animals had subclinical mastitis. Eighty-four CNS isolates were identified by MALDI-TOF MS and cydB real-time PCR (qPCR) and then evaluated by qPCR for presence of the eta, etb, sea, sec, cna, seb, sei, seq, sem, seg, see, and tst toxin genes, adhesion- and biofilm-associated genes (eno, ebps, fib, fnbA, coa), and the methicillin resistance gene (mecA). Resistance to antibiotics commonly used for mastitis treatment in Brazil was determined using the Kirby-Bauer test. Two strains were positive for the see and eta toxin genes; and mecA (1), eno (27), ebps (10), fnbA (10), and coa (5) genes were also detected. A notable number of isolates were resistant to erythromycin (30), penicillin (26), and cotrimoxazole (18); importantly, 10 vancomycin-resistant isolates were also detected. A smaller number of isolates were resistant to rifampicin (8), oxacillin (7), clindamycin (5), cefepime (4), tetracycline (3), ciprofloxacin (2), and chloramphenicol (1), and none were resistant to gentamicin or ciprofloxacin. Isolates with resistance to 2 (13 isolates), 3 (3), 4 (3), 5 (1), and 6 (1) antibiotics were detected. Taken together, our findings suggest that CNS isolates may not be a significant cause of clinical or even subclinical mastitis in buffaloes, but they may be a reservoir of virulence and antibiotic resistance genes.
650 $aAntibiotic resistance
650 $aBiofilm
653 $aDairy buffalo
653 $aVirulence genes
700 1 $aALMEIDA, C. C. de
700 1 $aSOLTES, G. A.
700 1 $aSLAVIC, D.
700 1 $aÁVILA, F. A. de
700 1 $aZAFALON, L. F.
700 1 $aMACINNES, J. I.
773 $tJournal of Dairy Science$gv.102, n.12, dec. 2019.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Amazônia Ocidental; Embrapa Amazônia Oriental. |
|
Data corrente: |
21/09/2016 |
|
Data da última atualização: |
03/10/2016 |
|
Tipo da produção científica: |
Documentos |
|
Autoria: |
SILVA, M. B. de S. W.; RODRIGUES, M. do R. L.; ROCHA, R. N. C. da; CRAVO, M. da S.; CORDEIRO, A. C. C.; BOARI, A. de J.; MENEZES, A. J. E. A. de; ARAGAO, D. V.; SILVA, E. S. A.; SANTOS, J. C. dos; MAUES, M. M.; OLIVEIRA, M. E. C.; MODESTO JUNIOR, M. de S.; ALVES, R. N. B.; FRANZINI, V. I.; GOMES JUNIOR, R. A. |
|
Afiliação: |
MAZILLENE BORGES DE SOUZA, CPATU; MARIA DO ROSARIO LOBATO RODRIGUES, CPAA; RAIMUNDO NONATO CARVALHO DA ROCHA, CPAA; MANOEL DA SILVA CRAVO, Aposentado CPATU; ANTONIO CARLOS CENTENO CORDEIRO, CPAF-Roraima; ALESSANDRA DE JESUS BOARI, CPATU; ANTONIO JOSE ELIAS A DE MENEZES, CPATU; DEBORA VEIGA ARAGAO, CPATU; ENILSON SOLANO ALBUQUERQUE SILVA, CPATU; JAIR CARVALHO DOS SANTOS, CPATU; MARCIA MOTTA MAUES, CPATU; MARCOS ENE CHAVES OLIVEIRA, CPATU; MOISES DE SOUZA MODESTO JUNIOR, CPATU; RAIMUNDO NONATO BRABO ALVES, CPATU; VINICIUS IDE FRANZINI, CPATU; RUI ALBERTO GOMES JUNIOR, CPATU. |
|
Título: |
Cultivo intercalar de culturas alimentares com palma de óleo na fase pré-produtiva. |
|
Ano de publicação: |
2016 |
|
Fonte/Imprenta: |
Belém, PA: Embrapa Amazônia Oriental, 2016. |
|
Páginas: |
30 p. |
|
Série: |
(Embrapa Amazônia Oriental. Documentos, 422). |
|
Idioma: |
Português |
|
Conteúdo: |
A Embrapa Amazônia Oriental, por meio das experiências conduzidas pelas Unidades sediadas no Pará, Amazonas e Roraima, apresenta esta Nota Técnica objetivando responder à pergunta: ?É possível intercalar palma de óleo com culturas alimentares??. |
|
Palavras-Chave: |
Palma de óleo. |
|
Thesagro: |
Cultivo Intercalado; Dendê; Planta Oleaginosa; Produção de Alimentos; Sistema de Cultivo. |
|
Categoria do assunto: |
A Sistemas de Cultivo |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/148219/1/DOCUMENTOS-422-Ainfo.pdf
|
|
Marc: |
LEADER 01414nam a2200385 a 4500
001 2053254
005 2016-10-03
008 2016 bl uuuu u0uu1 u #d
100 1 $aSILVA, M. B. de S. W.
245 $aCultivo intercalar de culturas alimentares com palma de óleo na fase pré-produtiva.$h[electronic resource]
260 $aBelém, PA: Embrapa Amazônia Oriental$c2016
300 $a30 p.
490 $a(Embrapa Amazônia Oriental. Documentos, 422).
520 $aA Embrapa Amazônia Oriental, por meio das experiências conduzidas pelas Unidades sediadas no Pará, Amazonas e Roraima, apresenta esta Nota Técnica objetivando responder à pergunta: ?É possível intercalar palma de óleo com culturas alimentares??.
650 $aCultivo Intercalado
650 $aDendê
650 $aPlanta Oleaginosa
650 $aProdução de Alimentos
650 $aSistema de Cultivo
653 $aPalma de óleo
700 1 $aRODRIGUES, M. do R. L.
700 1 $aROCHA, R. N. C. da
700 1 $aCRAVO, M. da S.
700 1 $aCORDEIRO, A. C. C.
700 1 $aBOARI, A. de J.
700 1 $aMENEZES, A. J. E. A. de
700 1 $aARAGAO, D. V.
700 1 $aSILVA, E. S. A.
700 1 $aSANTOS, J. C. dos
700 1 $aMAUES, M. M.
700 1 $aOLIVEIRA, M. E. C.
700 1 $aMODESTO JUNIOR, M. de S.
700 1 $aALVES, R. N. B.
700 1 $aFRANZINI, V. I.
700 1 $aGOMES JUNIOR, R. A.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Amazônia Oriental (CPATU) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
