Data/hora: 03/06/2024 16:45:17

Biblioteca(s): | Embrapa Cerrados.
Data corrente: | 11/02/2015
Data da última atualização: | 11/02/2015
Autoria: | SILVA, M. A. A.; SOUZA, M. M.; SILVA, G. S.; MELO, C. A. F.; CORRÊA, R. X.; ARAÚJO, I. S.; CONCEIÇÃO, L. D. H. C. S. da.
Afiliação: | LEO DUC HAA CARSON SCHWARTZHAUPT DA, CPAC.
Título: | Analysis of transferability of microsatellite primers (SSR) in wild Passiflora species and intraspecific genetic diversity in Passiflora alata.
Ano de publicação: | 2014
Fonte/Imprenta: | Genetics and Molecular Research, v. 13, n. 3, p. 5908-5918, 2014.
Idioma: | Inglês
Conteúdo: | ABSTRACT. The genus Passiflora L. is the most representative of Passifloraceae, with over 500 known species, among which 150-200 originated from Brazil. In addition to the great commercial importance of this genus for the fruit market, many of the species have exotic flowers with a huge diversity of colors and can thereby be exploited as ornamental plants. This study was aimed at investigating the transferability of microsatellite primers in wild Passiflora species (P. cacao, P. cincinnata, P. glandulosa, P. gibertii, and P. mucronata) and characterizing 29 P. alata accessions using microsatellite primers that were previously developed in a library enriched with microsatellites from P. edulis f. flavicarpa for P. alata. The interspecies cross-amplification rate varied, and P. cacao exhibited the highest rate of amplification, suggesting a greater degree of proximity to P. edulis. The study of intraspecific accessions in P. alata found genetic similarity, with values ranging from 0.47 to 1.00 and an average similarity of 0.74. Hence, this study revealed the intraspecific genetic variability of P. alata in the Universidade Estadual de Santa Cruz?s Active Germplasm Bank and will lead to the adoption of mating strategies between accessions; thus making their use more suitable for breeding purposes.
Palavras-Chave: | Diversidade genética; Marcador microsatelite; Transferabilidade de primers.
Thesagro: | Flor; Maracujá; Marcador genético; Melhoramento genético vegetal.
Thesaurus Nal: | DNA primers; Genetic variation; Microsatellite repeats; Passiflora alata.
Categoria do assunto: | G Melhoramento Genético
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/117856/1/Analysis-of-transferability-Leo.pdf
Registro original: | Embrapa Cerrados (CPAC)
Exemplares: | CPAC - (34385-1) - UPC - AP - PP - S1884 -  - S1884 - DIS