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| Registros recuperados : 30 | |
| 6. |  | MALUF, M. P.; SILVESTRINI, M.; RUGGIERO, L. M. de C.; GUERREIRO FILHO, O.; COLOMBO, C. A. Genetic diversity of cultivated Coffea arabica inbred lines assessed by RAPD, AFLP and SSR marker systems. Scientia Agricola, v. 62, n. 4, p. 366-373, 2005. Título em português: Caracterização da diversidade genética de linhagens comerciais de Coffea arabica através de marcadores moleculares do tipo RAPD, AFLP e SSR.| Biblioteca(s): Embrapa Café. |
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| 7. | | SILVESTRINI, M.; MALUF, M. P.; RUGGIERO, L. M. de C.; GUERREIRO-FILHO, O.; COLOMBO, C. A. Caracterização de linhagens comerciais de café através de marcadores moleculares. IN: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 1., 2000, Poços de Caldas. Resumos Expandidos. Brasília, DF: Embrapa Café/MINASPLAN, 2000.| Biblioteca(s): Embrapa Café. |
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| 9. | | ANDRADE, A. C.; VIEIRA, L. G. E.; COLOMBO, C. A.; PEREIRA, G. A. G. Coffee functional genomics in Brazil. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 21., 2006, Montpellier, France. Table of contents... Montpellier, France: Association for Science and Information on Coffee, 2006.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 10. | | ANDRADE, A. C.; VIEIRA, L. G. E.; COLOMBO, C. A.; PEREIRA, G. A. G. Coffee functional genomics in Brazil. INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 21., 2006, Montpellier, France. Table of contents... Montpellier, France: Association for Science and Information on Coffee, 2006.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 12. | | NOBILE, P. M.; QUECINI, V.; BAZZO, B.; QUITERIO, G.; MAZZAFERA, P.; COLOMBO, C. A. Transcriptional profile of genes involved in the byosynthesis of phytate and ferritin in coffea. Journal of Agricultural and Food Chemistry, Washington DC, v. 58, n. 6, p. 3479-3487, mar. 2010.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 13. | | CHIORATO, A. F.; CARBONELL, S. A. M.; COLOMBO, C. A.; DIAS, L. A. dos S.; ITO, M. F. Genetic diversity of common bean accessions to the germplasm bank of the Insituto Agronômico - IAC. Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 5, n. 1, p. 1-9, Mar. 2005.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 14. | | SECOND, G.; ALLEM, A. C.; MENDES, R. A.; CARVALHO, L. J. C. B.; EMPERAIRE, L.; INGRAM, C.; COLOMBO, C. Molecular marker (AFLP)-based Manihot and cassava numerical taxonomy and genetic structure analysis in progress: Implications for their dynamic conservation and genetic mapping. African Journal of Root and Tuber Crops, v.2, n.1/2, p.140-147, 1997. Producao Cientifica/CENARGEN/1997| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 16. | | SAWAZAKI, H. E.; SA, L. A. N. de; GONÇALVES, C. R. N. C. B.; VEIGA, R. F. A.; COLOMBO, C. A. Molecular diagnosis optimization of virus, bacteria and fungi in sugarcane. Internacional Research Journal of Plant Science, Sapele, v. 4, n. 3, p. 76-83, 2013.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 17. | | GIMENES, M. A.; PRIOLLI, R.; LIMA, P.; COLOMBO, C.; PEREIRA NETO, L. G.; CURI, C. C. S.; SCHMIDT, A.; PAIS, V. de O.; WETZEL, M. Monitoramento da coleção de germoplasma semente de soja mantido na coleção de base da Embrapa por meio de marcadores microssatélites. SIMPÓSIO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2008, Brasília, DF. Anais... Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia: Fundação de Apoio à Pesquisa Científica e Tecnológica - FUNCREDI, 2008. p.371.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 18. | | VENANCIO, M. M. H.; KIIHL, T. A. M.; SIMON, R. de A.; PUTTINI, F. de A.; NICOMEDES JÚNIOR, J.; COLOMBO, C. A. Caracterização agro-morfológica de acessos elite de mamona (Ricinus communis) do banco de germoplasma do Instituto Agronômico de Campinas. CONGRESSO BRASILEIRO DE MAMONA, 5.; SIMPÓSIO INTERNACIONAL DE OLEAGINOSAS ENERGÉTICAS, 2.; FÓRUM CAPIXABA DE PINHÃO-MANSO, 1., 2012, Guarapari. Desafios e Oportunidades: anais. Campina Grande: Embrapa Algodão, 2012. p. 356| Biblioteca(s): Embrapa Algodão. |
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| 19. | | VILELLA, O. T.; LANNES, S. D.; POT, D.; FERREIRA, L. P.; PRIOLLI, R. H. G.; RAMOS, L. C. S.; COLOMBO, C. A.; VIEIRA, L. G. E. Obtenção de marcadores moleculares por meio de PCR-RFLP de genes relacionados com qualidade em café. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 6., 2009, Vitória. Resumos expandidos. Brasília, DF: Embrapa Café.| Biblioteca(s): Embrapa Café. |
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| 20. | | SAWAZAKI, H. E.; SÁ, L. A. N. de; ALCÂNTARA, M. Q.; POLEZ, V. L. P.; GONÇALVES, C. R. N. C. B.; VEIGA, R. F. A.; COLOMBO, C. A. Otimização da diagnose molecular de vírus, bactéria e fungo em cana-de-açúcar. Biológico, São Paulo, v. 73, n. 2, p. 282-287, 2011. Anais da 24ª Reunião Anual do Instituto Biológico, São Paulo, nov. 2011.| Biblioteca(s): Embrapa Meio Ambiente. |
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| Registros recuperados : 30 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Café; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
18/02/2011 |
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Data da última atualização: |
28/02/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
VIDAL, R. O.; MONDEGO, J. M. C.; POT, D.; AMBRÓSIO, A. B.; ANDRADE, A. C.; PEREIRA, L. F. P.; COLOMBO, C. A.; VIEIRA, L. G. E.; CARAZZOLLE, M. F.; PEREIRA, G. A. G. |
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Afiliação: |
RAMON OLIVEIRA VIDAL, UNICAMP/Instituto de Biologia; JORGE MAURÍCIO COSTA MONDEGO, Instituto Agronômico de Campinas; DAVID POT, Centre de Coopération Internationale en Recherche Agronomique pour le Développement; ALINNE BATISTA AMBRÓSIO, UNICAMP/Instituto de Biologia; ALAN CARVALHO ANDRADE, CENARGEN; LUIZ FILIPE PROTASIO PEREIRA, SAPC; CARLOS AUGUSTO COLOMBO, Instituto Agronômico de Campinas; LUIZ GONZAGA ESTEVES VIEIRA, Instituo Agronômico do Pará; MARCELO FALSARELLA CARAZZOLLE, UNICAMP/Instituto de Biologia; GONÇALO AMARANTE GUIMARÃES PEREIRA, UNICAMP/Instituto de Biologia. |
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Título: |
A high-throughput data mining of single nucleotide polymorphisms in Coffea species expresed sequence tags suggests differential homeologous gene expression in the allotetrapoloid Coffea arabica. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
PLANT PHYSIOLOGY, v. 154, p. 1053-1066. 2010. |
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Páginas: |
1053-1066 |
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Idioma: |
Inglês |
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Conteúdo: |
Polyploidization constitutes a common mode of evolution in flowering plants. This event provides the raw material for the divergence of function in homeologous genes, leading to phenotypic novelty that can contribute to the success of polyploids in nature or their selection for use in agriculture. Mounting evidence underlined the existence of homeologous expression biases in polyploid genomes; however, strategies to analyze such transcriptome regulation remained scarce. Important factors regarding homeologous expression biases remain to be explored, such as whether this phenomenon influences specific genes, how paralogs are affected by genome doubling, and what is the importance of the variability of homeologous expression bias to genotype differences. This study reports the expressed sequence tag assembly of the allopolyploid Coffea arabica and one of its direct ancestors, Coffea canephora. The assembly was used for the discovery of single nucleotide polymorphisms through the identification of high-quality discrepancies in overlapped expressed sequence tags and for gene expression information indirectly estimated by the transcript redundancy. Sequence diversity profiles were evaluated within C. arabica (Ca) and C. canephora (Cc) and used to deduce the transcript contribution of the Coffea eugenioides (Ce) ancestor. The assignment of the C. arabica haplotypes to the C. canephora (CaCc) or C. eugenioides (CaCe) ancestral genomes allowed us to analyze gene expression contributions of each subgenome in C. arabica. In silico data were validated by the quantitative polymerase chain reaction and allele-specific combination TaqMAMA-based method. The presence of differential expression of C. arabica homeologous genes and its implications in coffee gene expression, ontology, and physiology are discussed. MenosPolyploidization constitutes a common mode of evolution in flowering plants. This event provides the raw material for the divergence of function in homeologous genes, leading to phenotypic novelty that can contribute to the success of polyploids in nature or their selection for use in agriculture. Mounting evidence underlined the existence of homeologous expression biases in polyploid genomes; however, strategies to analyze such transcriptome regulation remained scarce. Important factors regarding homeologous expression biases remain to be explored, such as whether this phenomenon influences specific genes, how paralogs are affected by genome doubling, and what is the importance of the variability of homeologous expression bias to genotype differences. This study reports the expressed sequence tag assembly of the allopolyploid Coffea arabica and one of its direct ancestors, Coffea canephora. The assembly was used for the discovery of single nucleotide polymorphisms through the identification of high-quality discrepancies in overlapped expressed sequence tags and for gene expression information indirectly estimated by the transcript redundancy. Sequence diversity profiles were evaluated within C. arabica (Ca) and C. canephora (Cc) and used to deduce the transcript contribution of the Coffea eugenioides (Ce) ancestor. The assignment of the C. arabica haplotypes to the C. canephora (CaCc) or C. eugenioides (CaCe) ancestral genomes allowed us to analyze gene expression contribut... Mostrar Tudo |
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Thesagro: |
Coffea Arábica. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/29342/1/A-high-throughput.pdf
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Marc: |
LEADER 02657naa a2200253 a 4500
001 1917245
005 2023-02-28
008 2010 bl uuuu u00u1 u #d
100 1 $aVIDAL, R. O.
245 $aA high-throughput data mining of single nucleotide polymorphisms in Coffea species expresed sequence tags suggests differential homeologous gene expression in the allotetrapoloid Coffea arabica.$h[electronic resource]
260 $c2010
300 $a1053-1066
520 $aPolyploidization constitutes a common mode of evolution in flowering plants. This event provides the raw material for the divergence of function in homeologous genes, leading to phenotypic novelty that can contribute to the success of polyploids in nature or their selection for use in agriculture. Mounting evidence underlined the existence of homeologous expression biases in polyploid genomes; however, strategies to analyze such transcriptome regulation remained scarce. Important factors regarding homeologous expression biases remain to be explored, such as whether this phenomenon influences specific genes, how paralogs are affected by genome doubling, and what is the importance of the variability of homeologous expression bias to genotype differences. This study reports the expressed sequence tag assembly of the allopolyploid Coffea arabica and one of its direct ancestors, Coffea canephora. The assembly was used for the discovery of single nucleotide polymorphisms through the identification of high-quality discrepancies in overlapped expressed sequence tags and for gene expression information indirectly estimated by the transcript redundancy. Sequence diversity profiles were evaluated within C. arabica (Ca) and C. canephora (Cc) and used to deduce the transcript contribution of the Coffea eugenioides (Ce) ancestor. The assignment of the C. arabica haplotypes to the C. canephora (CaCc) or C. eugenioides (CaCe) ancestral genomes allowed us to analyze gene expression contributions of each subgenome in C. arabica. In silico data were validated by the quantitative polymerase chain reaction and allele-specific combination TaqMAMA-based method. The presence of differential expression of C. arabica homeologous genes and its implications in coffee gene expression, ontology, and physiology are discussed.
650 $aCoffea Arábica
700 1 $aMONDEGO, J. M. C.
700 1 $aPOT, D.
700 1 $aAMBRÓSIO, A. B.
700 1 $aANDRADE, A. C.
700 1 $aPEREIRA, L. F. P.
700 1 $aCOLOMBO, C. A.
700 1 $aVIEIRA, L. G. E.
700 1 $aCARAZZOLLE, M. F.
700 1 $aPEREIRA, G. A. G.
773 $tPLANT PHYSIOLOGY$gv. 154, p. 1053-1066. 2010.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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