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Registros recuperados : 96 | |
21. | | BORTOLIN, A.; AOUADA, F. A.; LONGO, E.; MATTOSO, L. H. C. Influência da CMC no grau de intumescimento de hidrogéis constituídos por PAAm e ácido PMAA. In: JORNADA CIENTÍFICA - EMBRAPA SÃO CARLOS, 2., 2010, São Carlos, SP. Anais... São Carlos: Embrapa Instrumentação Agropecuária: Embrapa Pecuária Sudeste, 2010. p. 87. (Embrapa Instrumentação Agropecuária. Documentos, 50). Biblioteca(s): Embrapa Instrumentação. |
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28. | | BORTOLIN, A.; AOUADA, F. A.; MATTOSO, L. H. C.; OLIVEIRA, C. R. de. Liberação lenta/controlada de nutrientes por meio de hidrogéis nanocompósitos modificados com altos teores de argilomineral. In: WORKSHOP DA REDE DE NANOTECNOLOGIA APLICADA AO AGRONEGÓCIO, 9., 2017, São Carlos. Anais ... São Carlos: Embrapa Instrumentação, 2017. p. 508-511. Editores: Caue Ribeiro de Oliveira, Elaine Cristina Paris, Luiz Henrique Capparelli Mattoso, Marcelo Porto Bemquerer, Maria Alice Martins, Odílio Benedito Garrido de Assis. Biblioteca(s): Embrapa Instrumentação. |
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29. | | SERAFIM, A. R. T.; BORTOLIN, A.; AOUADA, F. A.; RIBEIRO, C.; MATTOSO, L. H. C. Liberação lenta de nutrientes a partir de nanocompósitos carregados com argilominerais. In: WORKSHOP DA REDE DE NANOTECNOLOGIA APLICADA AO AGRONEGÓCIO, 8, 2014, Juiz de fora. Anais... São Carlos: Embrapa Instrumentação; Campo Grande: Embrapa Gado de Corte; Juiz de Fora: Embrapa Gado de Leite, 2014. p. 484-488. Editores: Maria Alice Martins, Humberto de Mello Brandão, Marlene de Barros Coelho, Daniel Souza Corrêa, Caue Ribeiro, Luiz Henrique Capparelli Mattoso. Biblioteca(s): Embrapa Instrumentação. |
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31. | | NASCIMENTO, D. W. S.; MATTOSO, L. H. C.; MOURA, M. R.; AOUADA, F. A. Efeito de adição de argila na capacidade de absorção de água em hidrogéis híbridos nanoestruturados. In: WORKSHOP DA REDE DE NANOTECNOLOGIA APLICADA AO AGRONEGÓCIO, 9., 2017, São Carlos. Anais ... São Carlos: Embrapa Instrumentação, 2017. p. 535-538. Editores: Caue Ribeiro de Oliveira, Elaine Cristina Paris, Luiz Henrique Capparelli Mattoso, Marcelo Porto Bemquerer, Maria Alice Martins, Odílio Benedito Garrido de Assis. Biblioteca(s): Embrapa Instrumentação. |
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33. | | ARAUJO, H. A. G. B. de; MATTOSO, L. H. C.; AOUADA, F. A. Otimização das condições de síntese de hidrogéis nanocompósitos biodegradáveis. In: WORKSHOP DA REDE DE NANOTECNOLOGIA APLICADA AO AGRONEGÓCIO, 8, 2014, Juiz de fora. Anais... São Carlos: Embrapa Instrumentação; Campo Grande: Embrapa Gado de Corte; Juiz de Fora: Embrapa Gado de Leite, 2014. p. 448-451. Editores: Maria Alice Martins, Humberto de Mello Brandão, Marlene de Barros Coelho, Daniel Souza Corrêa, Caue Ribeiro, Luiz Henrique Capparelli Mattoso. Biblioteca(s): Embrapa Instrumentação. |
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35. | | LOREVICE, M. V.; MOURA, M. R. de; AOUADA, F. A.; MATTOSO, L. H. C. Novos filmes comestíveis contendo polpa de goiaba e nanoparticulas de quitosana. In: WORKSHOP DA REDE DE NANOTECNOLOGIA APLICADA AO AGRONEGÓCIO, 6., 2012, Fortaleza. Anais... São Carlos: Embrapa Instrumentação; Fortaleza: Embrapa Agroindústria Tropical, 2012. p. 129-131. Editores: Maria Alice Martins, MOrsyleide de Freitas Rosa, Men de Sá Moreira de Souza Filho, Nicodemos Moreira dos Santos Junior, Odílio Benedito Garrido de Assis, Caue Ribeiro, Luiz Henrique Capparelli Mattoso. Biblioteca(s): Embrapa Instrumentação. |
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37. | | BORTOLIN, A.; AOUADA, F. A.; RIBEIRO, C.; LONGO, E.; MATTOSO, L. H. C. Sorção e dessorção de insumos agrícolas carreados a partir de matrizes poliméricas porosas biodegradáveis. In: JORNADA CIENTÍFICA-EMBRAPA SÃO CARLOS, 2009, São Carlos, SP. Anais... São Carlos: Embrapa Pecuária Sudeste: Embrapa Instrumentação Agropecuária, 2009. p. 98 (Embrapa Pecuária Sudeste. Documentos, 90) Biblioteca(s): Embrapa Instrumentação. |
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39. | | AOUADA, F. A.; PAN, Z.; ORTS, W. J.; MATTOSO, L. H. C. Effect of acrylamide concentration on adsorption of paraquat pesticide onto PAAm-MC hydrogels. In: ENCONTRO DA SOCIEDADE BRASILEIRA DE PESQUISA EM MATERIAIS - SBPMat, 7., 2008, Guarujá; BRAZILIAN MRS MEETING, 7., 2008, Guarujá. Abstracts... Rio de Janeiro: SBPMat, 2008. não paginado. 1CD-ROM. Biblioteca(s): Embrapa Instrumentação. |
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Registros recuperados : 96 | |
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Registro Completo
Biblioteca(s): |
Embrapa Suínos e Aves; Embrapa Unidades Centrais. |
Data corrente: |
11/04/2024 |
Data da última atualização: |
11/04/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
VAZ, C. S. L.; FONSECA, F. M. da; RECH, D. V.; MORES, M. A. Z.; COLDEBELLA, A.; CANTAO, M. E. |
Afiliação: |
CLARISSA SILVEIRA LUIZ VAZ, CNPSA; FRANCISCO NOE DA FONSECA, GEM; DAIANE VOSS RECH, CNPSA; MARCOS ANTONIO ZANELLA MORES, CNPSA; ARLEI COLDEBELLA, CNPSA; MAURICIO EGIDIO CANTAO, CNPSA. |
Título: |
Wild-type lytic bacteriophages against Salmonella Heidelberg: further characterization and effect of prophylactic therapy in broiler chickens. |
Ano de publicação: |
2024 |
Fonte/Imprenta: |
Research in Veterinary Science, v. 171, n. 105247, 2024. |
DOI: |
https://doi.org/10.1016/j.rvsc.2024.105247 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: To characterize wild-type bacteriophages and their effect on Salmonella Heidelberg intestinal colonization in broilers, phages combined in a cocktail were continuously delivered via drinking water since the first day after hatching. After challenge with a field strain, broilers were evaluated at regular intervals for S. Heidelberg and bacteriophages in tissues and cecum, and gross and microscopic lesions in organs. Phages were highly virulent against S. Heidelberg by efficiency of plating. One-step growth curves exhibited eclipse period from 20 to 25 min, whereas the lowest latent period and higher burst size found were 45 min and 54 PFU/cell, respectively. Bacteriophage whole genomic sequencing analyses revealed a lack of genes related to lysogeny, antimicrobial resistance, and virulence factors. Relevant gross or microscopic lesions were absent in tissues analyzed from treated broilers. Although numerically stable bacteriophage concentrations were detected in the cecal contents of treated broilers, no significant difference was found for the S. Heidelberg cecal load in comparison to the untreated group and for the prevalence of positive tissues throughout the evaluated period. The phages produced turbid plaques against some S. Heidelberg re-isolated from treated broilers, suggesting the evolving of a resistant subpopulation. Overall, the results provide new evidence of the safety and in vitro replication of such phages in S. Heidelberg. Nevertheless, continuous administration of the phage suspension most likely induced the development of bacteriophage-resistant mutants, which might have affected the in vivo effect. Therefore, a putative administration protocol should be based on other strategies, such as short-term therapy at pre-harvest age. MenosAbstract: To characterize wild-type bacteriophages and their effect on Salmonella Heidelberg intestinal colonization in broilers, phages combined in a cocktail were continuously delivered via drinking water since the first day after hatching. After challenge with a field strain, broilers were evaluated at regular intervals for S. Heidelberg and bacteriophages in tissues and cecum, and gross and microscopic lesions in organs. Phages were highly virulent against S. Heidelberg by efficiency of plating. One-step growth curves exhibited eclipse period from 20 to 25 min, whereas the lowest latent period and higher burst size found were 45 min and 54 PFU/cell, respectively. Bacteriophage whole genomic sequencing analyses revealed a lack of genes related to lysogeny, antimicrobial resistance, and virulence factors. Relevant gross or microscopic lesions were absent in tissues analyzed from treated broilers. Although numerically stable bacteriophage concentrations were detected in the cecal contents of treated broilers, no significant difference was found for the S. Heidelberg cecal load in comparison to the untreated group and for the prevalence of positive tissues throughout the evaluated period. The phages produced turbid plaques against some S. Heidelberg re-isolated from treated broilers, suggesting the evolving of a resistant subpopulation. Overall, the results provide new evidence of the safety and in vitro replication of such phages in S. Heidelberg. Nevertheless, continuous a... Mostrar Tudo |
Palavras-Chave: |
Análise genômica; Bacteriófago; Foodborne pathogen; Genomic analysis; Patógeno de origem alimentar; Terapia fágica. |
Thesagro: |
Frango de Corte; Salmonelose. |
Thesaurus NAL: |
Bacteriophages; Phage therapy; Poultry; Salmonellosis. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02834naa a2200337 a 4500 001 2163526 005 2024-04-11 008 2024 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.rvsc.2024.105247$2DOI 100 1 $aVAZ, C. S. L. 245 $aWild-type lytic bacteriophages against Salmonella Heidelberg$bfurther characterization and effect of prophylactic therapy in broiler chickens.$h[electronic resource] 260 $c2024 520 $aAbstract: To characterize wild-type bacteriophages and their effect on Salmonella Heidelberg intestinal colonization in broilers, phages combined in a cocktail were continuously delivered via drinking water since the first day after hatching. After challenge with a field strain, broilers were evaluated at regular intervals for S. Heidelberg and bacteriophages in tissues and cecum, and gross and microscopic lesions in organs. Phages were highly virulent against S. Heidelberg by efficiency of plating. One-step growth curves exhibited eclipse period from 20 to 25 min, whereas the lowest latent period and higher burst size found were 45 min and 54 PFU/cell, respectively. Bacteriophage whole genomic sequencing analyses revealed a lack of genes related to lysogeny, antimicrobial resistance, and virulence factors. Relevant gross or microscopic lesions were absent in tissues analyzed from treated broilers. Although numerically stable bacteriophage concentrations were detected in the cecal contents of treated broilers, no significant difference was found for the S. Heidelberg cecal load in comparison to the untreated group and for the prevalence of positive tissues throughout the evaluated period. The phages produced turbid plaques against some S. Heidelberg re-isolated from treated broilers, suggesting the evolving of a resistant subpopulation. Overall, the results provide new evidence of the safety and in vitro replication of such phages in S. Heidelberg. Nevertheless, continuous administration of the phage suspension most likely induced the development of bacteriophage-resistant mutants, which might have affected the in vivo effect. Therefore, a putative administration protocol should be based on other strategies, such as short-term therapy at pre-harvest age. 650 $aBacteriophages 650 $aPhage therapy 650 $aPoultry 650 $aSalmonellosis 650 $aFrango de Corte 650 $aSalmonelose 653 $aAnálise genômica 653 $aBacteriófago 653 $aFoodborne pathogen 653 $aGenomic analysis 653 $aPatógeno de origem alimentar 653 $aTerapia fágica 700 1 $aFONSECA, F. M. da 700 1 $aRECH, D. V. 700 1 $aMORES, M. A. Z. 700 1 $aCOLDEBELLA, A. 700 1 $aCANTAO, M. E. 773 $tResearch in Veterinary Science$gv. 171, n. 105247, 2024.
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