Data/hora: 12/06/2024 19:42:37

Biblioteca(s): 	Embrapa Suínos e Aves; Embrapa Unidades Centrais.	
Data corrente: 	11/04/2024	
Data da última atualização: 	11/04/2024	
Autoria: 	VAZ, C. S. L.; FONSECA, F. M. da; RECH, D. V.; MORES, M. A. Z.; COLDEBELLA, A.; CANTAO, M. E.	
Afiliação: 	CLARISSA SILVEIRA LUIZ VAZ, CNPSA; FRANCISCO NOE DA FONSECA, GEM; DAIANE VOSS RECH, CNPSA; MARCOS ANTONIO ZANELLA MORES, CNPSA; ARLEI COLDEBELLA, CNPSA; MAURICIO EGIDIO CANTAO, CNPSA.	
Título: 	Wild-type lytic bacteriophages against Salmonella Heidelberg: further characterization and effect of prophylactic therapy in broiler chickens.	
Ano de publicação: 	2024	
Fonte/Imprenta: 	Research in Veterinary Science, v. 171, n. 105247, 2024.	
DOI: 	https://doi.org/10.1016/j.rvsc.2024.105247	
Idioma: 	Inglês	
Conteúdo: 	Abstract: To characterize wild-type bacteriophages and their effect on Salmonella Heidelberg intestinal colonization in broilers, phages combined in a cocktail were continuously delivered via drinking water since the first day after hatching. After challenge with a field strain, broilers were evaluated at regular intervals for S. Heidelberg and bacteriophages in tissues and cecum, and gross and microscopic lesions in organs. Phages were highly virulent against S. Heidelberg by efficiency of plating. One-step growth curves exhibited eclipse period from 20 to 25 min, whereas the lowest latent period and higher burst size found were 45 min and 54 PFU/cell, respectively. Bacteriophage whole genomic sequencing analyses revealed a lack of genes related to lysogeny, antimicrobial resistance, and virulence factors. Relevant gross or microscopic lesions were absent in tissues analyzed from treated broilers. Although numerically stable bacteriophage concentrations were detected in the cecal contents of treated broilers, no significant difference was found for the S. Heidelberg cecal load in comparison to the untreated group and for the prevalence of positive tissues throughout the evaluated period. The phages produced turbid plaques against some S. Heidelberg re-isolated from treated broilers, suggesting the evolving of a resistant subpopulation. Overall, the results provide new evidence of the safety and in vitro replication of such phages in S. Heidelberg. Nevertheless, continuous administration of the phage suspension most likely induced the development of bacteriophage-resistant mutants, which might have affected the in vivo effect. Therefore, a putative administration protocol should be based on other strategies, such as short-term therapy at pre-harvest age.	
Palavras-Chave: 	Análise genômica; Bacteriófago; Foodborne pathogen; Genomic analysis; Patógeno de origem alimentar; Terapia fágica.	
Thesagro: 	Frango de Corte; Salmonelose.	
Thesaurus Nal: 	Bacteriophages; Phage therapy; Poultry; Salmonellosis.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Suínos e Aves (CNPSA)	
Exemplares: 	CNPSA - (22658-1) - UPC - AP - DD -  -  -  - DIS
AI-SEDE - (67531-1) - UPC - AP - DD -  -  -  - DIS