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| Registros recuperados : 200 | |
| 9. |  | KRUG, L. D.; CHIARI, L.; JANK, L.; LAURA, V. A. Avaliação do transcriptoma de Panicum maximum Jacq. em resposta ao déficit hídrico. In: JORNADA CIENTÍFICA EMBRAPA GADO DE CORTE, 10., 2014, Campo Grande, MS. [Anais da..]. Campo Grande, MS: Embrapa Gado de Corte, 2014. p. 62-63. 2 p. (Embrapa Gado de Corte. Documentos, 208). Comissão organizadora: Grácia Maria Soares Rosinha, Alexandra Rocha de Oliveir, Rodrigo Carvalho Alva.| Biblioteca(s): Embrapa Gado de Corte. |
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| 15. | | CHAIA, G.; CHIARI, L.; SILVA, D. C.; GUEREIRO, J. Closantel (R 31.520) no tratamento da Dermatobia hominis (Lineu Jr., 1781). Pesquisa Agropecuária Brasileira, Brasília, DF, v. 16, n. 2, p. 193-197, mar. 1981 Título em inglês: Closantel (R 31.520) in the treatment of "bernes" (Dermatobia hominis) (Lineu Jr. 1781).| Biblioteca(s): Embrapa Unidades Centrais. |
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| 18. | | FERREIRA, R. C. U. (; CHIARI, L.; SOUZA, A. P. Transferibilidade de marcadores microssatélites entre espécies do gênero Urochloa. WORKSHOP MELHORAMENTO VEGETAL, 2., 2016, Campo Grande, MS. Contribuições, Avanços e perspectivas para o Cerrado brasileiro. Anais... Campo Grande, MS: SBMP, 2016.| Biblioteca(s): Embrapa Gado de Corte. |
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| 19. | | LEGUIZAMON, G. O. de C.; CHIARI, L.; JANK, L. Uso de RAPD para confirmação da identidade de genótipos de Panicum maximum Jacq. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 5., 2009, Guarapari. O melhoramento e os novos cenários da agricultura: anais. Vitória: Incaper, 2009. 1 CD-ROM. (Incaper. Documentos, 11). CBMP 2009. Organizado por Romário Gava Ferrão, Frederico de Pina Matta, Maria Amélia Gava Ferrão, João Cândido de Souza, Adelaide de F. S. da Costa, Liliâm Maria Ventorim Ferrão. 4 p. 1 CD-ROM.| Biblioteca(s): Embrapa Gado de Corte. |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
17/12/2018 |
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Data da última atualização: |
17/12/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 3 |
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Autoria: |
SOUZA, J. S.; CHIARI, L.; RESENDE, R. M. S.; VILELA, M. de M.; SALGADO, L. R. |
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Afiliação: |
Juliana Santos Souza, Universidade Anhanguera - Uniderp/Campus Agrárias; LUCIMARA CHIARI, CNPGC; ROSANGELA MARIA SIMEAO, CNPGC; MARIANE DE MENDONCA VILELA, CNPGC; Leonardo Rippel Salgado, CNPGC. |
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Título: |
Development, Validation and Characterization of Genic Microsatellite Markers in Urochloa Species. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
American Journal of Plant Science, v. 18, n. 3, p. 314-319, July/Sept. 2018. |
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DOI: |
DOI: 10.4236/ajps.2018.92023 |
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Idioma: |
Inglês |
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Conteúdo: |
Urochloa (syn. Brachiaria ) cultivars represent 85% of tropical pastures occupying 114 million hectares of cultivated grasslands in Brazil. Despite the commercial importance of the Urochloa species, low molecular information is available and is far from saturating the genome. Investigating and obtaining more markers associated to characteristics of difficult and late expression can benefit in accelerating breeding programs of more important species. Aiming to increase the number of molecular markers, genic microsatellite markers were obtained from transcriptome of U. decumbens and analyzed for their cross-amplification to U. brizantha , U. humidicola and U. ruziziensis . Genic microsatellite markers were isolated from a transcriptome obtained of U. decumbens ?Basilisk? roots. Specific primers were designed for one hundred loci, and 32 were polymorphic presenting polymorphism informative content values ranging of 0.12 to 0.81 (mean 0.54). Amplified microsatellite regions yield an average of 4.44 alleles per locus (ranging of 1 to 13). Cross-amplification to U. brizantha , U. humidicola and U. ruziziensis were successfully performed, although the number of the loci transferred varied among them. Multiple orrespondence Analysis revealed three distinct groups separating accessions and species. Four genetic markers presented high potential to distinguish sexual and apomictic accessions of Urochloa and must be further investigated. The genic markers identified in this study are the first set of expressed sequence tagged molecular markers for Urochloa species. MenosUrochloa (syn. Brachiaria ) cultivars represent 85% of tropical pastures occupying 114 million hectares of cultivated grasslands in Brazil. Despite the commercial importance of the Urochloa species, low molecular information is available and is far from saturating the genome. Investigating and obtaining more markers associated to characteristics of difficult and late expression can benefit in accelerating breeding programs of more important species. Aiming to increase the number of molecular markers, genic microsatellite markers were obtained from transcriptome of U. decumbens and analyzed for their cross-amplification to U. brizantha , U. humidicola and U. ruziziensis . Genic microsatellite markers were isolated from a transcriptome obtained of U. decumbens ?Basilisk? roots. Specific primers were designed for one hundred loci, and 32 were polymorphic presenting polymorphism informative content values ranging of 0.12 to 0.81 (mean 0.54). Amplified microsatellite regions yield an average of 4.44 alleles per locus (ranging of 1 to 13). Cross-amplification to U. brizantha , U. humidicola and U. ruziziensis were successfully performed, although the number of the loci transferred varied among them. Multiple orrespondence Analysis revealed three distinct groups separating accessions and species. Four genetic markers presented high potential to distinguish sexual and apomictic accessions of Urochloa and must be further investigated. The genic markers identified in this study are th... Mostrar Tudo |
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Palavras-Chave: |
Signalgrass; Simple Sequence Repeat; Transferability. |
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Thesagro: |
Brachiaria. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/188572/1/8-Development-validation-and-characterization.pdf
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Marc: |
LEADER 02299naa a2200229 a 4500
001 2101685
005 2018-12-17
008 2018 bl uuuu u00u1 u #d
024 7 $aDOI: 10.4236/ajps.2018.92023$2DOI
100 1 $aSOUZA, J. S.
245 $aDevelopment, Validation and Characterization of Genic Microsatellite Markers in Urochloa Species.$h[electronic resource]
260 $c2018
520 $aUrochloa (syn. Brachiaria ) cultivars represent 85% of tropical pastures occupying 114 million hectares of cultivated grasslands in Brazil. Despite the commercial importance of the Urochloa species, low molecular information is available and is far from saturating the genome. Investigating and obtaining more markers associated to characteristics of difficult and late expression can benefit in accelerating breeding programs of more important species. Aiming to increase the number of molecular markers, genic microsatellite markers were obtained from transcriptome of U. decumbens and analyzed for their cross-amplification to U. brizantha , U. humidicola and U. ruziziensis . Genic microsatellite markers were isolated from a transcriptome obtained of U. decumbens ?Basilisk? roots. Specific primers were designed for one hundred loci, and 32 were polymorphic presenting polymorphism informative content values ranging of 0.12 to 0.81 (mean 0.54). Amplified microsatellite regions yield an average of 4.44 alleles per locus (ranging of 1 to 13). Cross-amplification to U. brizantha , U. humidicola and U. ruziziensis were successfully performed, although the number of the loci transferred varied among them. Multiple orrespondence Analysis revealed three distinct groups separating accessions and species. Four genetic markers presented high potential to distinguish sexual and apomictic accessions of Urochloa and must be further investigated. The genic markers identified in this study are the first set of expressed sequence tagged molecular markers for Urochloa species.
650 $aBrachiaria
653 $aSignalgrass
653 $aSimple Sequence Repeat
653 $aTransferability
700 1 $aCHIARI, L.
700 1 $aRESENDE, R. M. S.
700 1 $aVILELA, M. de M.
700 1 $aSALGADO, L. R.
773 $tAmerican Journal of Plant Science$gv. 18, n. 3, p. 314-319, July/Sept. 2018.
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