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Registros recuperados : 540 | |
164. | | SANTOS, M. da C.; LEDO, A. da S.; GOMES, K. K. P. Efeito sa sabarose e do sorbitol na conservação in vitro de mangabeira. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS; WORKSHOP EM BIOPROSPECÇÃO E CONSERVAÇÃO DE PLANTAS NATIVAS DO SEMI-ÁRIDO, 3.; WORKSHOP INTERNACIONAL SOBRE BIOENERGIA E MEIO AMBIENTE, 2010, Salvador. Bancos de germoplasma: descobrir a riqueza, garantir o futuro: anais. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2010. 1 CD-ROM. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 304). Editora técnica Clara Oliveira Goedert. Resumo simpples Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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165. | | SANTOS, M. da C.; SÁ, A. de J.; LEDO, A. da S. Efeito do manitol, do acesso e tempo de cultivo na conservação in vitro de plântulas de mangabeira (Hancornia speciosa Gomes). In: CONGRESSO BRASILEIRO DE FLORICULTURA E PLANTAS ORNAMENTAIS, 17.; CONGRESSO BRASILEIRO DE CULTURA DE TECIDOS DE PLANTAS, 4., 2009, Aracaju. Ciência, inovação e sustentabilidade: anais. Aracaju: Embrapa Tabuleiros Costeiros, 2009. 1 CD-ROM. Resumo. Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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170. | | GOMES, K. K. P.; LEDO, A. da S.; SANTOS, M. da C. Efeito de métodos de dessecamento no teor de umidade de embriões zigóticos maduros de coqueiro. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS; WORKSHOP EM BIOPROSPECÇÃO E CONSERVAÇÃO DE PLANTAS NATIVAS DO SEMI-ÁRIDO, 3.; WORKSHOP INTERNACIONAL SOBRE BIOENERGIA E MEIO AMBIENTE, 2010, Salvador. Bancos de germoplasma: descobrir a riqueza, garantir o futuro: anais. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2010. 1 CD-ROM. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 304). Editora técnica Clara Oliveira Goedert. Resumo simples. Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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171. | | LEDO, A. da S.; CUNHA, A. O.; ARAGÃO, W. M.; TUPINAMBÁ, E. A. Efeito da sacarose e do manitol na conservação in vitro por cresciemento lento de coqueiro anão. Magistra, Cruz das Almas, v, 19, n. 4, p. 346-351, out./dez. 2007. Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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177. | | GOMES, K. K. P.; LEDO, A. da S.; SANTOS, M. da C. Desempenho de metodologias de desidratação de embriões zigóticos de coco (Cocos nucifera L.) para criopreservação. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS; WORKSHOP EM BIOPROSPECÇÃO E CONSERVAÇÃO DE PLANTAS NATIVAS DO SEMI-ÁRIDO, 3.; WORKSHOP INTERNACIONAL SOBRE BIOENERGIA E MEIO AMBIENTE, 2010, Salvador. Bancos de germoplasma: descobrir a riqueza, garantir o futuro: anais. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2010. 1 CD-ROM. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 304). Editora técnica Clara Oliveira Goedert. Resumo simples. Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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Registros recuperados : 540 | |
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Registro Completo
Biblioteca(s): |
Embrapa Gado de Corte. |
Data corrente: |
10/11/2017 |
Data da última atualização: |
17/04/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
SALGADO, L. R.; LIMA, R.; SANTOS, B. F. dos; SHIRAKAWA, K. T.; VILELA, M. de M.; ALMEIDA, N. F.; PEREIRA, R. M.; NEPOMUCENO, A. L.; CHIARI, L. |
Afiliação: |
LEONARDO RIPPEL SALGADO, Embrapa Beef Cattle, Campo Grande, Mato Grosso do Sul, Brazil; RODOLPHO LIMA, Federal University of Grande Dourados, Dourados, Mato Grosso do Sul, Brazil; BRUNO FERREIRA DOS SANTOS, Federal University of Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil; KARINA TAMIE SHIRAKAWA, Federal University of Grande Dourados, Dourados, Mato Grosso do Sul, Brazil; MARIANE DE MENDONCA VILELA, CNPGC; NALVO FRANCO ALMEIDA, Federal University of Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil; RODRIGO MATHEUS PEREIRA, Federal University of Grande Dourados, Dourados, Mato Grosso do Sul, Brazil; ALEXANDRE LIMA NEPOMUCENO, CNPSO; LUCIMARA CHIARI, CNPGC. |
Título: |
De novo RNA sequencing and analysis of the transcriptome of signalgrass (Urochloa decumbens) roots exposed to aluminum. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Plant Growth Regulation, v. 83, n. 1, p. 157-170, 2017. |
DOI: |
10.1007/s10725-017-0291-2 |
Idioma: |
Inglês |
Conteúdo: |
Acidic soils occupy a vast area in the world, and the aluminum (Al) in these soils can directly interact with plant cells and tissues to inhibit their growth and reduce yields. The signalgrass Urochloa decumbens cv. Basilisk (syn. Brachiaria decumbens cv. Basilisk), a widely sown tropical forage grass, is recognized for its high productivity under intensive use, vigorous growth, ease of establishment, and good forage value throughout the year, as well as its exceptional adaptation to infertile acid soils. We sequenced the transcriptome from roots of U. decumbens cv. Basilisk under two conditions, with and without Al, using Illumina paired-end sequencing technology and performed de novo assembly of those reads, which yielded 164,920 transcripts. Of these transcripts, 113,918 were assigned a putative protein function through comparisons with different gene set databases. Additionally, 13,375 simple sequence repeat (SSR) markers were identified. Digital gene expression analyses were conducted to identify 6698 differentially expressed genes between treatments, revealing a great differences in the root transcriptional landscape when exposed to aluminum. An extensive annotation of the differentially expressed genes (DEGs), made possible to identify several transcripts with putative functions correlated to aluminum exposure, most belonging to vesicle transportation, cell wall modifications and metal handling ontologies. In this work, abundant, high-quality transcripts were obtained, providing a reference platform for future biotechnological studies and breeding programs for this species and its close relatives. MenosAcidic soils occupy a vast area in the world, and the aluminum (Al) in these soils can directly interact with plant cells and tissues to inhibit their growth and reduce yields. The signalgrass Urochloa decumbens cv. Basilisk (syn. Brachiaria decumbens cv. Basilisk), a widely sown tropical forage grass, is recognized for its high productivity under intensive use, vigorous growth, ease of establishment, and good forage value throughout the year, as well as its exceptional adaptation to infertile acid soils. We sequenced the transcriptome from roots of U. decumbens cv. Basilisk under two conditions, with and without Al, using Illumina paired-end sequencing technology and performed de novo assembly of those reads, which yielded 164,920 transcripts. Of these transcripts, 113,918 were assigned a putative protein function through comparisons with different gene set databases. Additionally, 13,375 simple sequence repeat (SSR) markers were identified. Digital gene expression analyses were conducted to identify 6698 differentially expressed genes between treatments, revealing a great differences in the root transcriptional landscape when exposed to aluminum. An extensive annotation of the differentially expressed genes (DEGs), made possible to identify several transcripts with putative functions correlated to aluminum exposure, most belonging to vesicle transportation, cell wall modifications and metal handling ontologies. In this work, abundant, high-quality transcripts were obtained... Mostrar Tudo |
Palavras-Chave: |
Molecular markers; RNA sequencing; Urochloa genus. |
Thesagro: |
Brachiaria Decumbens. |
Thesaurus NAL: |
Aluminum. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/166583/1/De-novo-RNA-sequencing-and-analysis.pdf
|
Marc: |
LEADER 02506naa a2200289 a 4500 001 2079489 005 2018-04-17 008 2017 bl uuuu u00u1 u #d 024 7 $a10.1007/s10725-017-0291-2$2DOI 100 1 $aSALGADO, L. R. 245 $aDe novo RNA sequencing and analysis of the transcriptome of signalgrass (Urochloa decumbens) roots exposed to aluminum.$h[electronic resource] 260 $c2017 520 $aAcidic soils occupy a vast area in the world, and the aluminum (Al) in these soils can directly interact with plant cells and tissues to inhibit their growth and reduce yields. The signalgrass Urochloa decumbens cv. Basilisk (syn. Brachiaria decumbens cv. Basilisk), a widely sown tropical forage grass, is recognized for its high productivity under intensive use, vigorous growth, ease of establishment, and good forage value throughout the year, as well as its exceptional adaptation to infertile acid soils. We sequenced the transcriptome from roots of U. decumbens cv. Basilisk under two conditions, with and without Al, using Illumina paired-end sequencing technology and performed de novo assembly of those reads, which yielded 164,920 transcripts. Of these transcripts, 113,918 were assigned a putative protein function through comparisons with different gene set databases. Additionally, 13,375 simple sequence repeat (SSR) markers were identified. Digital gene expression analyses were conducted to identify 6698 differentially expressed genes between treatments, revealing a great differences in the root transcriptional landscape when exposed to aluminum. An extensive annotation of the differentially expressed genes (DEGs), made possible to identify several transcripts with putative functions correlated to aluminum exposure, most belonging to vesicle transportation, cell wall modifications and metal handling ontologies. In this work, abundant, high-quality transcripts were obtained, providing a reference platform for future biotechnological studies and breeding programs for this species and its close relatives. 650 $aAluminum 650 $aBrachiaria Decumbens 653 $aMolecular markers 653 $aRNA sequencing 653 $aUrochloa genus 700 1 $aLIMA, R. 700 1 $aSANTOS, B. F. dos 700 1 $aSHIRAKAWA, K. T. 700 1 $aVILELA, M. de M. 700 1 $aALMEIDA, N. F. 700 1 $aPEREIRA, R. M. 700 1 $aNEPOMUCENO, A. L. 700 1 $aCHIARI, L. 773 $tPlant Growth Regulation$gv. 83, n. 1, p. 157-170, 2017.
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