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Registro Completo |
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
12/07/2022 |
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Data da última atualização: |
03/01/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
BRANDÃO, M. da S.; MUNIZ, F.; HERNÁNDEZ-RANGEL, S.; CAMPOS, Z.; FARIAS, I.; HRBEK, T. |
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Afiliação: |
MAYNARA DA SILVA BRANDÃO, Federal University of Amazonas, UFAM, Manaus; FÁBIO MUNIZ, Federal University of Amazonas, UFAM, Manaus; SANDRA HERNÁNDEZ-RANGEL, Federal University of Amazonas, UFAM, Manaus; ZILCA MARIA DA SILVA CAMPOS, CPAP; IZENI FARIAS, Federal University of Amazonas, UFAM, Manaus; TOMAS HRBEK, Federal University of Amazonas, UFAM, Manaus. |
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Título: |
SNPs marker toolbox for molecular identification of nominal and candidate species of the genus Paleosuchus. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
In: WORKING MEETING OF THE CROCODILE SPECIALIST GROUP OF THE INTERNATIONAL UNION FOR CONSERVATION NATURE, 26., 2022, Chetumal. Abstracts... [Chetumal]: CSG, 2022. |
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Páginas: |
p. 123. |
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Idioma: |
Inglês |
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Notas: |
Evento Online. |
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Conteúdo: |
Recent studies have identified the existence of species complexes in crocodilians, including in the New World Caimaninae. Both species of the genus Paleosuchus are species complexes with three or four species subsumed under the P. palpebrosus epithet and two under the P. trigonatus epithet. From the conservation point of view, it is important to recognize the evolutionary and ecological uniqueness of these lineages, regardless of the taxonomic status they may receive. Candidate species of the genus Paleosuchus have not yet been formally described and therefore do not have diagnostic morphological characters that would allow their identification in nature. This problem motivated us to develop a SNP (Single Nucleotide Polymorphisms) toolkit that enables molecular identification of the nominal and candidate species of Paleosuchus. For this, a ddRADseq (Double Digest RAD-sequencing) protocol was used to obtain reduced representation genome of 20 P. palpebrosus samples, five from each of four lineages ('Amazonia', 'Bolivia', 'Madeira' and 'Pantanal'), and five samples of P. trigonatus, three from the 'Amazon' and two from the 'Guyana' lineage. We selected from between 11 and 15 private SNPs for each lineage that unambiguously assign individuals to one of these lineages. Additionally, we selected 11 mutually exclusive private SNPs that unambiguously assign individuals to one of the two species complexes. The use of the toolkit is affordable and fast-to-apply in comparison to other molecular identification methods, and so it will enable a rapid molecular assignment of individuals to the newly identified lineages of the genus, which may be suffering different degrees of threat. Additional applications of this marker set are: the assignment of zoo specimens, pet trade individuals, museum samples, or degraded samples to source lineages; molecular identification seized animal parts (eg. bush meat); and identification of the origin of illegally traded animals or their by-products (forensic genetics). MenosRecent studies have identified the existence of species complexes in crocodilians, including in the New World Caimaninae. Both species of the genus Paleosuchus are species complexes with three or four species subsumed under the P. palpebrosus epithet and two under the P. trigonatus epithet. From the conservation point of view, it is important to recognize the evolutionary and ecological uniqueness of these lineages, regardless of the taxonomic status they may receive. Candidate species of the genus Paleosuchus have not yet been formally described and therefore do not have diagnostic morphological characters that would allow their identification in nature. This problem motivated us to develop a SNP (Single Nucleotide Polymorphisms) toolkit that enables molecular identification of the nominal and candidate species of Paleosuchus. For this, a ddRADseq (Double Digest RAD-sequencing) protocol was used to obtain reduced representation genome of 20 P. palpebrosus samples, five from each of four lineages ('Amazonia', 'Bolivia', 'Madeira' and 'Pantanal'), and five samples of P. trigonatus, three from the 'Amazon' and two from the 'Guyana' lineage. We selected from between 11 and 15 private SNPs for each lineage that unambiguously assign individuals to one of these lineages. Additionally, we selected 11 mutually exclusive private SNPs that unambiguously assign individuals to one of the two species complexes. The use of the toolkit is affordable and fast-to-apply in comparison to other... Mostrar Tudo |
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Thesagro: |
Jacaré. |
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Thesaurus Nal: |
Caiman. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1144666/1/CSG26-Abstracts-Program-220707-151117-123.pdf
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Marc: |
LEADER 02768nam a2200217 a 4500
001 2144666
005 2023-01-03
008 2022 bl uuuu u00u1 u #d
100 1 $aBRANDÃO, M. da S.
245 $aSNPs marker toolbox for molecular identification of nominal and candidate species of the genus Paleosuchus.$h[electronic resource]
260 $aIn: WORKING MEETING OF THE CROCODILE SPECIALIST GROUP OF THE INTERNATIONAL UNION FOR CONSERVATION NATURE, 26., 2022, Chetumal. Abstracts... [Chetumal]: CSG$c2022
300 $ap. 123.
500 $aEvento Online.
520 $aRecent studies have identified the existence of species complexes in crocodilians, including in the New World Caimaninae. Both species of the genus Paleosuchus are species complexes with three or four species subsumed under the P. palpebrosus epithet and two under the P. trigonatus epithet. From the conservation point of view, it is important to recognize the evolutionary and ecological uniqueness of these lineages, regardless of the taxonomic status they may receive. Candidate species of the genus Paleosuchus have not yet been formally described and therefore do not have diagnostic morphological characters that would allow their identification in nature. This problem motivated us to develop a SNP (Single Nucleotide Polymorphisms) toolkit that enables molecular identification of the nominal and candidate species of Paleosuchus. For this, a ddRADseq (Double Digest RAD-sequencing) protocol was used to obtain reduced representation genome of 20 P. palpebrosus samples, five from each of four lineages ('Amazonia', 'Bolivia', 'Madeira' and 'Pantanal'), and five samples of P. trigonatus, three from the 'Amazon' and two from the 'Guyana' lineage. We selected from between 11 and 15 private SNPs for each lineage that unambiguously assign individuals to one of these lineages. Additionally, we selected 11 mutually exclusive private SNPs that unambiguously assign individuals to one of the two species complexes. The use of the toolkit is affordable and fast-to-apply in comparison to other molecular identification methods, and so it will enable a rapid molecular assignment of individuals to the newly identified lineages of the genus, which may be suffering different degrees of threat. Additional applications of this marker set are: the assignment of zoo specimens, pet trade individuals, museum samples, or degraded samples to source lineages; molecular identification seized animal parts (eg. bush meat); and identification of the origin of illegally traded animals or their by-products (forensic genetics).
650 $aCaiman
650 $aJacaré
700 1 $aMUNIZ, F.
700 1 $aHERNÁNDEZ-RANGEL, S.
700 1 $aCAMPOS, Z.
700 1 $aFARIAS, I.
700 1 $aHRBEK, T.
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Registro original: |
Embrapa Pantanal (CPAP) |
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| Registros recuperados : 92 | |
| 23. |  | SOUZA, M. M.; VILELA, M. de F.; CHAIB FILHO, H. Evolução da produção agrícola nas microrregiões do Bioma Cerrado. In: ENCONTRO DE JOVENS TALENTOS DA EMBRAPA CERRADOS, 4., 2009, Planaltina, DF. Resumos apresentados... Planaltina, DF: Embrapa Cerrados, 2009. p. 132-133 (Embrapa Cerrados. Documentos, 243).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Cerrados. |
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| 26. | | PIRES, R. G.; LÔBO, C. F.; CHAIB FILHO, H. Identificação dos produtos que constam nos trabalhos apresentados no Congresso Brasileiro de Plantas Oleaginosas, Óleos, Gorduras e Biodiesel. In: ENCONTRO DE JOVENS TALENTOS DA EMBRAPA CERRADOS, 4., 2009, Planaltina, DF. Resumos apresentados... Planaltina, DF: Embrapa Cerrados, 2009. p. 138-139 (Embrapa Cerrados. Documentos, 243).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Cerrados. |
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| 35. | | VILELA, N. J.; MOITA, A. W.; GARAGORRY, F. L.; CHAIB FILHO, H. Avaliação da dinâmica regional da produção brasileira de tomate no período de 1975 a 2003. Horticultura Brasileira, Brasilia, DF, v. 26, n. 2, p. S4868-S4875, 2008. Suplemento. CD-ROM. Trabalho apresentado no 48. Congresso Brasileiro de Olericultura, Maringá, 2008.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Hortaliças. |
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| 38. | | MITJA, D.; SOUSA-SILVA, J. C.; MELO, S. L. de; CHAIB FILHO, H. Biometria dos frutos e sementes de babaçu, Natividade-TO. In: SIMPÓSIO NACIONAL CERRADO, 9.; SIMPÓSIO INTERNACIONAL SAVANAS TROPICAIS, 2., 2008, Brasília, DF. Desafios e estratégias para o equilíbrio entre sociedade, agronegócio e recursos naturais: anais... Planaltina, DF: Embrapa Cerrados, 2008. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Cerrados. |
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| 40. | | GARAGORRY, F. L.; ABREU, U. G. P. de; CHAIB FILHO, H.; BARIONI, L. G. Concentração e movimentação da pecuária de corte no bioma Pantanal, de 1990 a 2006. SIMPÓSIO SOBRE RECURSOS NATURAIS E SOCIOECONÔMICOS DO PANTANAL, 5., 2010, Corumbá, MS. Anais... Corumbá: Embrapa Pantanal: UFMS; Campinas: ICS do Brasil, 2010. 1 CD-ROM SIMPAN 2010. Não Paginado.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Pantanal. |
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| Registros recuperados : 92 | |
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