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Registros recuperados : 28 | |
6. | | KITAJIMA, E. W.; TASSI, A. D.; SAITO, M. S. G.; SALAROLI, R. B.; CHABI-JESUS, C.; ASTUA, J. de F. Novas espécies hospedeiras do vírus da leprose do ligustro (Ligustrum leprosis virus-LigLV). In: CONGRESSO PAULISTA DE FITOPATOLOGIA, 40., 2017, Campinas. SP: Instituro Agronômico, 2017 Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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7. | | CHABI-JESUS, C.; RAMOS-GONZÁLEZ, P. L.; TASSI, A. D.; GUERRA-PERAZA, O.; KITAJIMA, E. W.; HARAKAVA, R.; BESERRA, JR. J. E. A.; ASTUA, J. de F. Identification and characterization of citrus chlorotic spot virus, a new dichorhavirus associated with citrus leprosis-like symptoms. Plant Disease, v.102, p. 1588-1598, 2018. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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8. | | SAUVÊTRE, P.; VENIANT, E.; CROQ, G.; TASSI, A. D.; KITAJIMA, E. W.; CHABI-JESUS, C.; RAMOS-GONZÁLEZ, P. L.; ASTUA, J. de F.; FERREIRA, D. N. M. First report of orchid fleck virus in the orchid collection of Jardin du Luxembourg, Paris, France. Plant Disease, v. 102, n. 12, p. 2670, 2018. Na publicação: J. Freitas-Astúa, D. Navia. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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10. | | CHABI-JESUS, C.; RAMOS-GONZÁLEZ, P. L.; TASSI, A. D.; BARGUIL, B. M.; BESERRA JUNIOR, J. E. A.; HARAKAVA, R.; KITAJIMA, E. W.; ASTUA, J. de F. First Report of Citrus Chlorotic Spot Virus Infecting the Succulent Plant Agave desmettiana. Plant Disease, v.103, n. 6, June 2019. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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12. | | RODRIGUES, M. da C.; RAMOS-GONZÁLEZ, P. L.; CHABI-JESUS, C.; HARAKAVA, R.; BARBOSA, C. de J.; COSTA, H.; ASTUA, J. de F. Primeiro relato de um higrevirus na área Continental Americana.first report of a Higrevirus in Mainland America. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 52., 2021. Anais... [Brasília, DF]: Sociedade Brasileira de Fitopatologia, 2021. p. 182. Evento online. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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13. | | RAMOS-GONZÁLEZ, P. L.; CHABI-JESUS, C.; BANGUELA-CASTILLO, E.; TASSI, A. D.; RODRIGUES, M. da C.; KITAJIMA, E. W.; HARAKAVA, R.; ASTUA, J. de F. Unveiling the complete genome sequence of clerodendrum chlorotic spot virus, a putative dichorhavirus infecting ornamental plants. Archives of Virology, 2018. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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14. | | CHABI-JESUS, C.; RAMOS-GONZALES, P.; CALEGARIO, R.; GUERRA-PERAZA, O.; TASSI, A.; KITAJIMA, E. W.; HARAKAVA, R.; ASTUA, J. de F. Complete genome sequence of clerodendrum chlorotic spot vírus, a putative dichorhavirus. In: SCIENCE PROTECTING PLANT HEALTH, Queensland, Australia, 2017. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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15. | | RAMOS-GONZÁLEZ, P. L.; CHABI-JESUS, C.; GUERRA-PERAZA, O.; TASSI, A. D.; KITAJIMA, E. W.; HARAKAVA, R.; SALAROLI, R. B.; ASTUA, J. de F. Citrus leprosis virus N: a new dichorhavirus causing citrus leprosis disease. Phytopathology, v.107, n.8, August 2017. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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16. | | DIETZGEN, R. G.; ASTUA, J. de F.; CHABI-JESUS, C.; RAMOS-GONZÁLEZ, P. L.; GOODIN, M. M.; KONDO, H.; TASSI, A. D.; KITAJIMA, E. W. Dichorhaviruses in their host plants and mite vectors. Advances in Virus Research, Elsevier, 2018. 30p. il. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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17. | | CHABI-JESUS C.; RAMOS-GONZÁLEZ, P. L.; GUERRA-PERAZA, O.; HARAKAVA, R.; KITAJIMA, E. W.; BASTIANEL, M.; ASTUA, J. de F. Disclosing three citrus leprosis-associated viruses of the new genus Dichorhavirus, family rhabdoviridae. In: INTERNATIONAL CITRUS CONGRESS, 2016, Foz do Iguaçu. Abstract book. Campinas: IAC; Londrina: IAPAR, 2016. p.215. Resumo S15-372. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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20. | | RAMOS-GONZÁLEZ, P. L.; CHABI-JESUS, C.; TASSI, A. D.; CALEGARIO, R. F.; HARAKAVA, R.; NOME, C F.; KITAJIMA, E. W.; ASTUA, J. de F. A Novel Lineage of Cile-Like Viruses Discloses the Phylogenetic Continuum Across the Family Kitaviridae. Frontiers in Microbiology, v.28, n.13, 836076, March 2022. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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Registros recuperados : 28 | |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
18/03/2020 |
Data da última atualização: |
02/06/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
RAMOS-GONZÁLEZ, P. L.; SANTOS, G, F. dos; CHABI-JESUS, C.; CHABI-JESUS, CAMILA; HARAKAVA, R.; KITAJIMA, E. W.; ASTUA, J. de F. |
Afiliação: |
PEDRO LUIS RAMOS-GONZÁLEZ, Instituto Biológico; GUSTAVO FRANCISCO DOS SANTOS, Instituto Biológico; CAMILA CHABI-JESUS, Instituto Biológico; CAMILA CHABI-JESUS, Instituto Biológico; RICARDO HARAKAVA, Instituto Biológico; ELLIOT W. KITAJIMA, ESALQ; JULIANA DE FREITAS ASTUA, CNPMF. |
Título: |
Passion fruit green spot virus genome harbors a new orphan ORF and highlights the flexibility of the 5'-end of the RNA2 segment across cileviruses. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Frontiers in Microbiology, February 2020 . |
DOI: |
https://doi.org/10.3389/fmicb.2020.00206 |
Idioma: |
Inglês |
Conteúdo: |
Passion fruit green spot and passion fruit sudden death are two reportedly distinct viral diseases that recurrently affect passion fruit (Passiflora spp.) groves in Brazil. Here we used a systematic approach that interconnects symptoms, transmission electron microscopy, RT-PCR detection assays followed by Sanger sequencing, and high-throughput sequencing of the RNA of affected passion fruit plants to gain insights about these diseases. Our data confirmed not only the involvement of cileviruses in these two pathologies, as previously suggested, but also that these viruses belong to the same tentative species: passion fruit green spot virus (PfGSV). Results revealed that PfGSV has a positive-sense RNA genome split into two molecules of approximately 9 kb (RNA1) and 5 kb (RNA2), which share about 50?70% nucleotide sequence identity with other viruses in the genus Cilevirus. Genome sequences of five PfGSV isolates suggest that they have more conserved RNA1 (<5% of nucleotide sequence variability) compared to RNA2 (up to 7% of variability) molecules. The highest nucleotide sequence divergence among PfGSV isolates and other cileviruses is in the genomic segment covering from the 5?-end of the RNA2 until the 5?-end of the open reading frame (ORF) p61, which includes the ORF p15 and the intergenic region. This genomic stretch also harbors a novel orphan ORF encoding a 13 kDa protein presenting a cysteine-rich domain. High variability of 5?-end of the RNA2 in cileviruses is discussed in an evolutionary context assuming that they share putative common ancestors with unclassified arthropod-infecting single-strand positive RNA viruses, including mosquito-specific viruses of the group Negevirus (clades Nelorpivirus and Sandwavirus), and other viruses in the family Kitaviridae. MenosPassion fruit green spot and passion fruit sudden death are two reportedly distinct viral diseases that recurrently affect passion fruit (Passiflora spp.) groves in Brazil. Here we used a systematic approach that interconnects symptoms, transmission electron microscopy, RT-PCR detection assays followed by Sanger sequencing, and high-throughput sequencing of the RNA of affected passion fruit plants to gain insights about these diseases. Our data confirmed not only the involvement of cileviruses in these two pathologies, as previously suggested, but also that these viruses belong to the same tentative species: passion fruit green spot virus (PfGSV). Results revealed that PfGSV has a positive-sense RNA genome split into two molecules of approximately 9 kb (RNA1) and 5 kb (RNA2), which share about 50?70% nucleotide sequence identity with other viruses in the genus Cilevirus. Genome sequences of five PfGSV isolates suggest that they have more conserved RNA1 (<5% of nucleotide sequence variability) compared to RNA2 (up to 7% of variability) molecules. The highest nucleotide sequence divergence among PfGSV isolates and other cileviruses is in the genomic segment covering from the 5?-end of the RNA2 until the 5?-end of the open reading frame (ORF) p61, which includes the ORF p15 and the intergenic region. This genomic stretch also harbors a novel orphan ORF encoding a 13 kDa protein presenting a cysteine-rich domain. High variability of 5?-end of the RNA2 in cileviruses is discussed... Mostrar Tudo |
Thesagro: |
Maracujá. |
Categoria do assunto: |
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Marc: |
LEADER 02524naa a2200217 a 4500 001 2121307 005 2020-06-02 008 2020 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.3389/fmicb.2020.00206$2DOI 100 1 $aRAMOS-GONZÁLEZ, P. L. 245 $aPassion fruit green spot virus genome harbors a new orphan ORF and highlights the flexibility of the 5'-end of the RNA2 segment across cileviruses.$h[electronic resource] 260 $c2020 520 $aPassion fruit green spot and passion fruit sudden death are two reportedly distinct viral diseases that recurrently affect passion fruit (Passiflora spp.) groves in Brazil. Here we used a systematic approach that interconnects symptoms, transmission electron microscopy, RT-PCR detection assays followed by Sanger sequencing, and high-throughput sequencing of the RNA of affected passion fruit plants to gain insights about these diseases. Our data confirmed not only the involvement of cileviruses in these two pathologies, as previously suggested, but also that these viruses belong to the same tentative species: passion fruit green spot virus (PfGSV). Results revealed that PfGSV has a positive-sense RNA genome split into two molecules of approximately 9 kb (RNA1) and 5 kb (RNA2), which share about 50?70% nucleotide sequence identity with other viruses in the genus Cilevirus. Genome sequences of five PfGSV isolates suggest that they have more conserved RNA1 (<5% of nucleotide sequence variability) compared to RNA2 (up to 7% of variability) molecules. The highest nucleotide sequence divergence among PfGSV isolates and other cileviruses is in the genomic segment covering from the 5?-end of the RNA2 until the 5?-end of the open reading frame (ORF) p61, which includes the ORF p15 and the intergenic region. This genomic stretch also harbors a novel orphan ORF encoding a 13 kDa protein presenting a cysteine-rich domain. High variability of 5?-end of the RNA2 in cileviruses is discussed in an evolutionary context assuming that they share putative common ancestors with unclassified arthropod-infecting single-strand positive RNA viruses, including mosquito-specific viruses of the group Negevirus (clades Nelorpivirus and Sandwavirus), and other viruses in the family Kitaviridae. 650 $aMaracujá 700 1 $aSANTOS, G, F. dos 700 1 $aCHABI-JESUS, C. 700 1 $aCHABI-JESUS, CAMILA 700 1 $aHARAKAVA, R. 700 1 $aKITAJIMA, E. W. 700 1 $aASTUA, J. de F. 773 $tFrontiers in Microbiology, February 2020 .
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