Data/hora: 23/06/2024 07:14:35

Biblioteca(s): | Embrapa Agricultura Digital.
Data corrente: | 22/12/2015
Data da última atualização: | 22/12/2015
Autoria: | MACHADO-DE-ÁVILA, R. A.; VELLOSO, M.; OLIVEIRA, D.; STRANSKY, S.; FLOR-SÁ, A.; SCHNEIDER, F. S.; NESHICH, G.; CHÁVEZ-OLÓRTEGUI, C.
Afiliação: | RICARDO ANDREZ MACHADO-DE-ÁVILA, Universidade do Extremo Sul Catarinense, Criciúma-SC; MARIANA VELLOSO, UFMG; DAYSIANE OLIVEIRA, UFMG; STEPHANIE STRANSKY, UFMG; ANA FLOR-SÁ, UFMG; FRANCISCO S. SCHNEIDER, UFMG; GORAN NESHICH, CNPTIA; CARLOS CHÁVEZ-OLÓRTEGUI, Universidade do Extremo Sul Catarinense, Criciúma-SC.
Título: | Induction of neutralizing antibodies against mutalysin-II from Lachesis muta muta Snake Venom Elicited by a conformational B-cell epitope predicted by Blue Star Sting data base.
Ano de publicação: | 2015
Fonte/Imprenta: | Immunome Research, v. 11, n. 1, Mar. 2015.
Páginas: | Não paginado.
DOI: | http://dx.doi.org/10.4172/1745-7580.1000083
Idioma: | Inglês
Conteúdo: | Mutalysin-II, from Lachesis muta muta snake venom, is an endopeptidase with hemorrhagic activity. To identify a conformational epitope we used Blue Star Sting, the latest version of the web based Sting Millenium Suite, as alternative computational analysis tool to select and design peptides. Pre-selected cut-off values of the accessibility and hydrophilicity parameters were used to select amino acid residues as potential conformational epitopes. A peptide (P117-Y116-C115-Q194-C195-L197-N198-K199-P200-Y5-L48) was manually drawn on Swiss-PDB-Viewer package and synthesized by Fmoc-synthesis. Immunization of rabbits with this peptide induced antibodies that recognized Mutalysin-II and protected against the hemorrhagic factors present in Lachesis venom. The Sting Millennium Suite was able to predict conformational epitopes in this class of proteins. Three amino acids (K199, Y5 and L48) were identified as essential in the interaction between the peptide and the neutralizing antibodies.
Palavras-Chave: | Conformational epitope prediction; Peptídeos; Sting millennium.
Thesagro: | Veneno.
Thesaurus Nal: | Antivenoms; Epitopes; Lachesis muta; Synthetic peptides.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/136041/1/induction-Avila.pdf
Registro original: | Embrapa Agricultura Digital (CNPTIA)
Exemplares: | CNPTIA - (18543-1) - UPC - AP - DD -  -  -  - DIS