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| Registros recuperados : 286 | |
| 165. |  | FUKUDA, W. M. G.; FIALHO, J. F.; CAVALCANTI, J.; CARDOSO, E. M. R.; BARRETO, J. F.; TERNES, M. Recursos genéticos de mandioca (Manihot esculenta Crantz) no Brasil. In: SIMPÓSIO DE RECURSOS GENÉTICOS PARA AMÉRICA LATINA E CARIBE, 3.; REUNIÃO LATINO AMERICANA DE ESPECIALISTAS EM ARACHIS, 3.; REUNIÃO LATINO AMERICANA DE ESPECIALISTAS EM RECURSOS GENÉTICOS FLORESTAIS, 3., 2001, Londrina. Anais... Londrina: IAPAR, 2001. p. 531-533.| Biblioteca(s): Embrapa Amazônia Ocidental; Embrapa Cerrados. |
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| 166. | | FUKUDA, W. M. G.; FIALHO, J. F.; CAVALCANTI, J.; CARDOSO, E. M. R.; BARRETO, J. F.; TERNES, M. Recursos genéticos de mandioca (Manihot esculenta Crantz) no Brasil. In: SIMPÓSIO DE RECURSOS GENÉTICOS PARA AMÉRICA LATINA E CARIBE, 3.; REUNIÃO LATINO AMERICANA DE ESPECIALISTAS EM ARACHIS, 3.; REUNIÃO LATINO AMERICANA DE ESPECIALISTAS EM RECURSOS GENÉTICOS FLORESTAIS, 3., 2001, Londrina. Anais... Londrina: IAPAR, 2001. p. 531-533.| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Semiárido. |
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| 167. | | CAVALCANTI, J. J. V.; SOUZA, F. X.; MARQUES, G. V.; BORDALLO, P. do N.; PINHEIRO, C. R. Repetibilidade de caracteres de desenvolvimento vegetativo e de produção de clones de cajazeira In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 20.; ANNUAL MEETING OF THE INTERAMERICAN SOCIETY FOR TROPICAL AGRICULTURE, 54., 2008, Vitória. Livro de resumos. Vitória: Incaper, 2008. 6 p.| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 173. | | QUEIROZ, D. R.; FARIAS, F. J. C.; CAVALCANTI, J. J. V.; CARVALHO, L. P. de; SOUZA, L. da S. S. de; ASSUNCAO, J. H. de. Análise dialélica para precocidade em genótipos de algodoeiro herbáceo. In: ENCONTRO DE GENÉTICA DO NORDESTE, 21., 2016, Recife. Anais... Ribeirão Preto: SBG; Recife: UFPE: UFRPE: UPE, 2016. p. 99.| Biblioteca(s): Embrapa Algodão. |
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| 174. | | FUKUDA, W. M. G.; CALDAS, R. C.; MAGALHAES, J. A.; CAVALCANTI, J.; TAVARES, J. A.; IGLESIAS, C.; HERNANDEZ, R. L. A. Analise de estabilidade de variedades de mandioca selecionadas atraves de provas participativas com produtores do semi-arido do Nordeste do Brasil. In: CONGRESSO BRASILEIRO DE MANDIOCA, 1.,1999, Manaus. Mandioca: sua importancia frente a globalizacao da economia - resumos. Cruz das Almas: SBM/Embrapa Mandioca e Fruticultura, 1999. p. 26.| Biblioteca(s): Embrapa Semiárido. |
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| 175. | | FUKUDA, W. M. G.; CALDAS, R. C.; MAGALHÃES, J. A.; CAVALCANTI, J.; TAVARES, J. A.; IGLESIAS, C.; HERNANDEZ ROMERO, L. A. Análise de estabilidade de variedades em mandioca selecionadas em provas participativas com produtores do semi-árido do Nordeste do Brasil. Revista Brasileira de Mandioca, v. 18, n. 1, p. 13-18, out. 2005.| Biblioteca(s): Embrapa Semiárido. |
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| 176. | | FUKUDA, W. M. G.; CALDAS, R. C.; MAGALHÃES, J. A.; CAVALCANTI, J.; TAVARES, J. A.; IGLESIAS, C.; ROMERO, L. A. H. Análise de estabilidade de variedades de mandioca selecionadas em provas participativas com produtores do Semi-Árido no Nordeste do Brasil. Revista Brasileira de Mandioca, Cruz das Almas, v.18, n. 1, p. 13-18, out. 2005.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 177. | | SOBREIRA JÚNIOR, O. V.; OLIVEIRA, K. G. de S.; BORDALLO, P. do N.; CASTRO, A. C. R. de; CAVALCANTI, J. J. V. Análise de caracteres morfológicos quantitativos do cajueiro do cerrado. In: CONGRESSO NACIONAL DE BOTÂNICA, 59.; REUNIÃO NORDESTINA DE BOTÂNICA, 31.; CONGRESSO LATINO AMERICANO Y DEL CARIBE DE CACTÁCEAS Y OTRAS SUCULENTAS, 4.; CONGRESS OF INTERNATIONAL ORGANIZATION FOR SUCULENT PLANT STUDY, 30., 2008, Natal. Atualidades, desafios e perspectivas da botânica no Brasil: anais. Natal: UFERSA: UFRN: SBB, 2008.| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 178. | | SOUZA, M. F. de; MIRANDA, O. C.; PAIVA, J. R. de; BARROS, L. de M.; CORRÊA, M. C. de M.; CAVALCANTI, J. J. J. V.; MELO, D. S. BRS Bahia 12: clone de cajueiro-anão precoce para plantio comercial no Município de Ribeira do Pombal, BA, e áreas similares. Fortaleza: Embrapa Agroindústria Tropical, 2004. 28 p. (Embrapa Agroindústria Tropical. Boletim de Pesquisa e Desenvolvimento, 17).| Biblioteca(s): Embrapa Semiárido. |
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| 179. | | SOUZA, M. F. de; MIRANDA, O. C.; PAIVA, J. R. de; BARROS, L. de M.; CORREA, M. C. de M.; CAVALCANTI, J. J. V.; MELO, D. S. BRS BAHIA 12: clone de cajueiro-anão precoce para plantio comercial no Município de Ribeira do Pombal, BA, e áreas similares. Fortaleza: Embrapa Agroindústria Tropical, 2004. 26 p. (Embrapa Agroindustria Tropical. Boletim de Pesquisa e Desenvolvimento, 17).| Biblioteca(s): Embrapa Agroindústria Tropical; Embrapa Algodão; Embrapa Mandioca e Fruticultura; Embrapa Unidades Centrais. |
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| 180. | | PAIVA, J. R. de; CAVALCANTI, J. J. V.; BARROS, L. de M.; CRISÓSTOMO, J. R.; LIMA, A. C.; CARDOSO, J. E.; MESQUITA, A. L. M.; MOSCA, J. L. BRS 274 (BRS Jacaju): common or giant cashew clone. Crop Breeding and Applied Biotechnology, v.8, p.236-238, 2008| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| Registros recuperados : 286 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
06/03/2017 |
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Data da última atualização: |
29/03/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SANTOS, R. C. dos; SILVA, C. R. C. da; SOARES, T. da C; CAVALCANTI, J. J. V.; LIMA, L. M. de; MARTINS, E. S.; MONNERAT, R. |
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Afiliação: |
ROSEANE CAVALCANTI DOS SANTOS, CNPA; CARLIANE REBECA COELHO da SILVA, UFRPE; TAÍZA da CUNHA SOARES, UFRPE; JOSE JAIME VASCONCELOS CAVALCANTI, CNPA; LIZIANE MARIA DE LIMA, CNPA; ÉRICA SOARES MARTINS, Cenargen; ROSE GOMES MONNERAT SOLON DE PONTES, Cenargen. |
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Título: |
Relative expression of CRy10 in GM cotton lines resistant to boll weevil. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
In: ENCONTRO DE GENÉTICA DO NORDESTE, 21., 2016, Recife. Anais... Ribeirão Preto: SBG; Recife: UFPE: UFRPE: UPE, 2016. p. 87. |
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Idioma: |
Inglês |
|
Conteúdo: |
Boll weevil (Anthonomus grandis) is a serious pest of cotton crop in several parts of world. The control is done by synthetic pesticides that increase the management costs. Embrapa coordinates an important research involving development of GM cotton resistant to boll weevil, by genetic transformation. Seven line (T0-8H) containing a Btcry 10 have been identified by feeding bioassays and ELISA, which showed reasonable toxicity against adult boll weevils. In this work, these lines were used in order to estimate the presence and relative expression of cry 10 by PCR and RT-qPCR, respectively, from young buds of 50d-plants. RT-qPCR assays were performed, by using Syber Green Rox Plus Master Mix 2X (LGC). Three endogenous cotton gene (GhACT, GhUBQ14 and GhPP2A) were used as a standard control. At first, 95°C/15 min and 40 cycles of 95°C/ 20 sec, 60°C/ 20 sec and 72°C/ 20 sec. Then, a curve of denaturation (melting curve) was performed after conclusion of amplification, at 95°C/15 sec, 60°C/15 sec, rising 2°/min until reaching 95°C. All reactions were carried out with experimental and biological replications. The threshold cycle (Ct) and PCR efficiency was estimated by Real-time PCR Miner program. The analyses were performed by using qBASEPlus program. Graphics, Cqs and Melt curve were automatically generated, based on the normalization method with a reference gene, ??Cq. Varied levels of expression were found in GM lines, too low in 8H-269 and 8H-357, mid in 8H-282 and 8H-346 and high in 8H- 336 (14x). These data agreed with previous results obtained by ELISA assays. Eleven lines derivate from 8H-336 (T1) were analyzed by PCR assays with genomic DNA, using 2 primer combinations. More than 50% showed amplicons confirming the presence of gene in selected lines. Taking in account that a reasonable level of resistance should overcoming 2 ug of protein/g tissue, we suggested that 8H-366 is the best genotype for control the cotton boll weevil. This material will be further advanced for entomological assays with larvae and adults of boll weevil. MenosBoll weevil (Anthonomus grandis) is a serious pest of cotton crop in several parts of world. The control is done by synthetic pesticides that increase the management costs. Embrapa coordinates an important research involving development of GM cotton resistant to boll weevil, by genetic transformation. Seven line (T0-8H) containing a Btcry 10 have been identified by feeding bioassays and ELISA, which showed reasonable toxicity against adult boll weevils. In this work, these lines were used in order to estimate the presence and relative expression of cry 10 by PCR and RT-qPCR, respectively, from young buds of 50d-plants. RT-qPCR assays were performed, by using Syber Green Rox Plus Master Mix 2X (LGC). Three endogenous cotton gene (GhACT, GhUBQ14 and GhPP2A) were used as a standard control. At first, 95°C/15 min and 40 cycles of 95°C/ 20 sec, 60°C/ 20 sec and 72°C/ 20 sec. Then, a curve of denaturation (melting curve) was performed after conclusion of amplification, at 95°C/15 sec, 60°C/15 sec, rising 2°/min until reaching 95°C. All reactions were carried out with experimental and biological replications. The threshold cycle (Ct) and PCR efficiency was estimated by Real-time PCR Miner program. The analyses were performed by using qBASEPlus program. Graphics, Cqs and Melt curve were automatically generated, based on the normalization method with a reference gene, ??Cq. Varied levels of expression were found in GM lines, too low in 8H-269 and 8H-357, mid in 8H-282 and 8H-346 and ... Mostrar Tudo |
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Palavras-Chave: |
Boll weevil. |
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Thesagro: |
Algodão; Anthonomus Grandis; Bicudo. |
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Thesaurus NAL: |
Cotton. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/157044/1/Relative-expression-of-Cry10.pdf
|
|
Marc: |
LEADER 02841nam a2200241 a 4500
001 2066203
005 2023-03-29
008 2016 bl uuuu u01u1 u #d
100 1 $aSANTOS, R. C. dos
245 $aRelative expression of CRy10 in GM cotton lines resistant to boll weevil.$h[electronic resource]
260 $aIn: ENCONTRO DE GENÉTICA DO NORDESTE, 21., 2016, Recife. Anais... Ribeirão Preto: SBG; Recife: UFPE: UFRPE: UPE, 2016. p. 87.$c2016
520 $aBoll weevil (Anthonomus grandis) is a serious pest of cotton crop in several parts of world. The control is done by synthetic pesticides that increase the management costs. Embrapa coordinates an important research involving development of GM cotton resistant to boll weevil, by genetic transformation. Seven line (T0-8H) containing a Btcry 10 have been identified by feeding bioassays and ELISA, which showed reasonable toxicity against adult boll weevils. In this work, these lines were used in order to estimate the presence and relative expression of cry 10 by PCR and RT-qPCR, respectively, from young buds of 50d-plants. RT-qPCR assays were performed, by using Syber Green Rox Plus Master Mix 2X (LGC). Three endogenous cotton gene (GhACT, GhUBQ14 and GhPP2A) were used as a standard control. At first, 95°C/15 min and 40 cycles of 95°C/ 20 sec, 60°C/ 20 sec and 72°C/ 20 sec. Then, a curve of denaturation (melting curve) was performed after conclusion of amplification, at 95°C/15 sec, 60°C/15 sec, rising 2°/min until reaching 95°C. All reactions were carried out with experimental and biological replications. The threshold cycle (Ct) and PCR efficiency was estimated by Real-time PCR Miner program. The analyses were performed by using qBASEPlus program. Graphics, Cqs and Melt curve were automatically generated, based on the normalization method with a reference gene, ??Cq. Varied levels of expression were found in GM lines, too low in 8H-269 and 8H-357, mid in 8H-282 and 8H-346 and high in 8H- 336 (14x). These data agreed with previous results obtained by ELISA assays. Eleven lines derivate from 8H-336 (T1) were analyzed by PCR assays with genomic DNA, using 2 primer combinations. More than 50% showed amplicons confirming the presence of gene in selected lines. Taking in account that a reasonable level of resistance should overcoming 2 ug of protein/g tissue, we suggested that 8H-366 is the best genotype for control the cotton boll weevil. This material will be further advanced for entomological assays with larvae and adults of boll weevil.
650 $aCotton
650 $aAlgodão
650 $aAnthonomus Grandis
650 $aBicudo
653 $aBoll weevil
700 1 $aSILVA, C. R. C. da
700 1 $aSOARES, T. da C
700 1 $aCAVALCANTI, J. J. V.
700 1 $aLIMA, L. M. de
700 1 $aMARTINS, E. S.
700 1 $aMONNERAT, R.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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