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Biblioteca(s):  Embrapa Soja.
Data corrente:  10/01/2005
Data da última atualização:  03/08/2017
Autoria:  GRANGE, L.; HUNGRIA, M.
Título:  Genetic diversity of indigenous common bean (Phaseolus vulgaris) rhizobia in two Brazilian ecosystems.
Ano de publicação:  2004
Fonte/Imprenta:  Soil Biology and Biochemistry, Oxford, v. 36, n. 9, p. 1389-1398, Sept. 2004.
Idioma:  Inglês
Conteúdo:  Although rhizobia for common bean (Phaseolus vulgaris L.) are established in most Brazilian soils, understanding of their genetic diversity is very poor. This study characterized bean strains from two contrasting ecosystems in Brazil, the Northeast Region, with a semi-arid climate and neutral soils and the South Region, with a humid subtropical climate and acid soils. Seedlings of the cultivars Negro Argel and Aporé were used to trap 243 rhizobial isolates from 12 out of 14 sites. An analysis of ERIC-PCR products revealed enormous variability, with 81% of the isolates representing unique strains considering a level of 70% of similarity. In general, there was no effect of either the bean cultivar, or the ecosystem on rhizobial diversity. One-hundred and one strains showing genetic relatedness (ERIC-PCR) less than 70% were further analyzed using restriction fragment length polymorphism (RFLP) of the 16 S rDNA cleaved with five restriction enzymes. Twenty-five different profile combinations were obtained. Rhizobium etli was the predominant species, with 73 strains showing similar RFLP profiles, while 12 other strains differed only by the profile with one restriction enzyme. Fifty strains were submitted to sequencing of a 16 S rDNA fragment, and 34 clustered with R. etli, including strains with RFLP-PCR profiles similar to those species or differing by one restriction enzyme. However, other strains differing by one or two enzymes were genetically distant from R. etli and two str... Mostrar Tudo
Palavras-Chave:  Rizóbio.
Thesagro:  Feijão.
Categoria do assunto:  --
Marc:  Mostrar Marc Completo
Registro original:  Embrapa Soja (CNPSO)
Biblioteca ID Origem Tipo/Formato Classificação Cutter Registro Volume Status URL
CNPSO24737 - 1UPCSP - --1018710187
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1.Imagem marcado/desmarcadoFONSECA, R. R. da; ORTIZ-RAMÍREZ, F. A.; CAVALCANTI, D. N.; RAMOS, C. J. B.; TEIXEIRA, V. L.; SOUSA FILHO, A. P. da S. Allelopathic potential of extracts the from marine macroalga Plocamium brasiliense and their effects on pasture weed. Revista Brasileira de Farmacognosia, v. 22, n. 4, p. 850-853, July/Aug. 2012.
Tipo: Artigo em Periódico IndexadoCirculação/Nível: B - 1
Biblioteca(s): Embrapa Amazônia Oriental.
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