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 | Acesso ao texto completo restrito à biblioteca da Embrapa Instrumentação. Para informações adicionais entre em contato com cnpdia.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
02/08/2019 |
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Data da última atualização: |
11/11/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
TADINI, A. M.; NICOLODELLI, G.; MARANGONI, B. S.; MOUNIER, S.; MONTES, C. R.; MILORI, D. M. B. P. |
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Afiliação: |
DEBORA MARCONDES BASTOS PEREIRA, CNPDIA. |
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Título: |
Evaluation of the roles of metals and humic fractions in the podzolization of soils from the Amazon region using two analytical spectroscopy techniques. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Microchemical Journal, v. 144, 2019. |
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Páginas: |
454-460 |
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ISSN: |
0026-265X |
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DOI: |
10.1016/j.microc.2018.10.009 |
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Idioma: |
Inglês |
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Palavras-Chave: |
Amazon; FAAS; LIBS. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00742naa a2200241 a 4500
001 2111088
005 2019-11-11
008 2019 bl uuuu u00u1 u #d
022 $a0026-265X
024 7 $a10.1016/j.microc.2018.10.009$2DOI
100 1 $aTADINI, A. M.
245 $aEvaluation of the roles of metals and humic fractions in the podzolization of soils from the Amazon region using two analytical spectroscopy techniques.$h[electronic resource]
260 $c2019
300 $a454-460
653 $aAmazon
653 $aFAAS
653 $aLIBS
700 1 $aNICOLODELLI, G.
700 1 $aMARANGONI, B. S.
700 1 $aMOUNIER, S.
700 1 $aMONTES, C. R.
700 1 $aMILORI, D. M. B. P.
773 $tMicrochemical Journal$gv. 144, 2019.
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Embrapa Instrumentação (CNPDIA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
23/02/2010 |
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Data da última atualização: |
15/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
MINGOTI, G. Z.; CAIADO CASTRO, V. S. D.; NICIURA, S. C. M.; BARRETTO, L. S. S.; GARCIA, J. M. |
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Afiliação: |
G. Z. MINGOTI, UNESP/ARAÇATUBA; V. S. D. CAIADO CASTRO, UNESP/JABOTICABAL; SIMONE CRISTINA MEO NICIURA, CPPSE; L. S. S. BARRETTO, UNESP/JABOTICABAL; J. M. GARCIA, UNESP/JABOTICABAL. |
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Título: |
The effect of interaction between macromolecule supplement and oxygen tension on bovine oocytes and embryos cultured in vitro. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Zygote, v. 17, n. 4, p. 321-328, may 2009. |
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DOI: |
10.1017/S0967199409005450 |
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Idioma: |
Inglês |
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Conteúdo: |
SummaryAiming to improve in vitro production of bovine embryos and to obtain supplements to replace serum for in vitro maturation (IVM), this study evaluated the effects of macromolecular supplementation of IMV medium (bovine serum albumin - BSA, polyvinyl alcohol - PVA, polyvinyl pyrrolidone - PVP, Ficoll, KnockoutSR, or fetal calf serum - FCS) and oxygen tension [5% CO2 in air (20% O2) or 5% CO2, 5% O2 and 90% N2 (5% O2)] on oocyte maturation and embryo development. Nuclear progression to germinal vesicle breakdown, metaphase I and metaphase II stages were evaluated and overall results revealed that undefined (FCS) and semi-defined (BSA) media gave better results at 20% O2 and defined media (PVA, PVP and Ficoll) at 5% O2. Independent of macromolecule supplement, IVM at 20% O2 was considered optimal for nuclear maturation. To evaluate embryo development, oocytes matured in the previously described conditions were fertilized and cultured at the same oxygen tension used for IVM and assessed for cleavage (43.0 to 74.8%) and development to morulae (16.4 to 33.8%), blastocyst (7.7 to 52.9%) and hatched blastocyst (9.6 to 48.1%). Apart from oxygen tension, all treatments, except Knockout (22.7%), gave similar results for blastocyst development (26.5 to 38.7%). Independently of macromolecule supplement, higher development rates were obtained in an oxygen tension of 20% O2 (67.4% cleavage, 29.2% morulae, 40.8% blastocyst and 34.0% hatched blastocyst) when compared with 5% O2 (52.5, 21.8, 18.2 and 15.6%, respectively). This study indicates that BSA, PVA, PVP and Ficoll can replace serum during IVM and that the optimal atmospheric condition for in vitro production of bovine embryos is 5% CO2 and 20% O2. MenosSummaryAiming to improve in vitro production of bovine embryos and to obtain supplements to replace serum for in vitro maturation (IVM), this study evaluated the effects of macromolecular supplementation of IMV medium (bovine serum albumin - BSA, polyvinyl alcohol - PVA, polyvinyl pyrrolidone - PVP, Ficoll, KnockoutSR, or fetal calf serum - FCS) and oxygen tension [5% CO2 in air (20% O2) or 5% CO2, 5% O2 and 90% N2 (5% O2)] on oocyte maturation and embryo development. Nuclear progression to germinal vesicle breakdown, metaphase I and metaphase II stages were evaluated and overall results revealed that undefined (FCS) and semi-defined (BSA) media gave better results at 20% O2 and defined media (PVA, PVP and Ficoll) at 5% O2. Independent of macromolecule supplement, IVM at 20% O2 was considered optimal for nuclear maturation. To evaluate embryo development, oocytes matured in the previously described conditions were fertilized and cultured at the same oxygen tension used for IVM and assessed for cleavage (43.0 to 74.8%) and development to morulae (16.4 to 33.8%), blastocyst (7.7 to 52.9%) and hatched blastocyst (9.6 to 48.1%). Apart from oxygen tension, all treatments, except Knockout (22.7%), gave similar results for blastocyst development (26.5 to 38.7%). Independently of macromolecule supplement, higher development rates were obtained in an oxygen tension of 20% O2 (67.4% cleavage, 29.2% morulae, 40.8% blastocyst and 34.0% hatched blastocyst) when compared with 5% O2 (52.5,... Mostrar Tudo |
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Palavras-Chave: |
Macromolecule. |
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Thesagro: |
Embrião; Gado de corte. |
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Thesaurus NAL: |
Animal breeding; Beef cattle; Oocytes. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02494naa a2200253 a 4500
001 1658493
005 2023-03-15
008 2009 bl uuuu u00u1 u #d
024 7 $a10.1017/S0967199409005450$2DOI
100 1 $aMINGOTI, G. Z.
245 $aThe effect of interaction between macromolecule supplement and oxygen tension on bovine oocytes and embryos cultured in vitro.$h[electronic resource]
260 $c2009
520 $aSummaryAiming to improve in vitro production of bovine embryos and to obtain supplements to replace serum for in vitro maturation (IVM), this study evaluated the effects of macromolecular supplementation of IMV medium (bovine serum albumin - BSA, polyvinyl alcohol - PVA, polyvinyl pyrrolidone - PVP, Ficoll, KnockoutSR, or fetal calf serum - FCS) and oxygen tension [5% CO2 in air (20% O2) or 5% CO2, 5% O2 and 90% N2 (5% O2)] on oocyte maturation and embryo development. Nuclear progression to germinal vesicle breakdown, metaphase I and metaphase II stages were evaluated and overall results revealed that undefined (FCS) and semi-defined (BSA) media gave better results at 20% O2 and defined media (PVA, PVP and Ficoll) at 5% O2. Independent of macromolecule supplement, IVM at 20% O2 was considered optimal for nuclear maturation. To evaluate embryo development, oocytes matured in the previously described conditions were fertilized and cultured at the same oxygen tension used for IVM and assessed for cleavage (43.0 to 74.8%) and development to morulae (16.4 to 33.8%), blastocyst (7.7 to 52.9%) and hatched blastocyst (9.6 to 48.1%). Apart from oxygen tension, all treatments, except Knockout (22.7%), gave similar results for blastocyst development (26.5 to 38.7%). Independently of macromolecule supplement, higher development rates were obtained in an oxygen tension of 20% O2 (67.4% cleavage, 29.2% morulae, 40.8% blastocyst and 34.0% hatched blastocyst) when compared with 5% O2 (52.5, 21.8, 18.2 and 15.6%, respectively). This study indicates that BSA, PVA, PVP and Ficoll can replace serum during IVM and that the optimal atmospheric condition for in vitro production of bovine embryos is 5% CO2 and 20% O2.
650 $aAnimal breeding
650 $aBeef cattle
650 $aOocytes
650 $aEmbrião
650 $aGado de corte
653 $aMacromolecule
700 1 $aCAIADO CASTRO, V. S. D.
700 1 $aNICIURA, S. C. M.
700 1 $aBARRETTO, L. S. S.
700 1 $aGARCIA, J. M.
773 $tZygote$gv. 17, n. 4, p. 321-328, may 2009.
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