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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
24/11/1999 |
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Data da última atualização: |
15/05/2023 |
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Autoria: |
AMIN, M. N.; JAISWAL, V. S. |
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Afiliação: |
Department of Botany, Banaras Hindu University, Varanasi IND 221 005, India. |
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Título: |
Rapid clonal propagation of guava through in vitro shoot proliferation on nodal explants of mature trees. |
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Ano de publicação: |
1987 |
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Fonte/Imprenta: |
Plant Cell, Tissue and Organ Culture, v.9, p.235-243, 1987 |
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Idioma: |
Inglês |
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Conteúdo: |
In vitro clonal propagation of guava `Banaras local' was achieved by culturing nodal explants of mature trees on Murashige and Skoog (MS) revised medium supplemented with 4.5 uM 6-benzyladanine (BA) alone or in combination with either 0.6 uM indole-3-acetic acid (IAA), 0.5 uM indole-3-butyric acid (IBA) or 0.3 uM gibberellic acid (GA3). Multiple shoots were induced to form by enhancement of axillary branching and BA (4.5 uM) without any auxin and gibberellin was found to give best shoot multiplication rate. In this medium 3-6 shoots developed on explants collected from field-grown plants and 5-10 shoots developed on explants taken from in vitro proliferated shoots within 12 wk of culture. a prior transfer of shoot clumps to a medium containing a lower concentration of BA (0.5 uM) before harvesting of cuttings for rooting allowed rapid extension growth and increased the number of usable shoots per culture. |
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Palavras-Chave: |
Cultura de tecidos; Gema axilar; Nodal explant; Psidium guajava L; Psidium guava L; Tropical fruit. |
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Thesagro: |
Fruta Tropical; Micropropagação. |
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Thesaurus Nal: |
micropropagation; tissue culture. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01676naa a2200253 a 4500
001 1648411
005 2023-05-15
008 1987 bl uuuu u00u1 u #d
100 1 $aAMIN, M. N.
245 $aRapid clonal propagation of guava through in vitro shoot proliferation on nodal explants of mature trees.$h[electronic resource]
260 $c1987
520 $aIn vitro clonal propagation of guava `Banaras local' was achieved by culturing nodal explants of mature trees on Murashige and Skoog (MS) revised medium supplemented with 4.5 uM 6-benzyladanine (BA) alone or in combination with either 0.6 uM indole-3-acetic acid (IAA), 0.5 uM indole-3-butyric acid (IBA) or 0.3 uM gibberellic acid (GA3). Multiple shoots were induced to form by enhancement of axillary branching and BA (4.5 uM) without any auxin and gibberellin was found to give best shoot multiplication rate. In this medium 3-6 shoots developed on explants collected from field-grown plants and 5-10 shoots developed on explants taken from in vitro proliferated shoots within 12 wk of culture. a prior transfer of shoot clumps to a medium containing a lower concentration of BA (0.5 uM) before harvesting of cuttings for rooting allowed rapid extension growth and increased the number of usable shoots per culture.
650 $amicropropagation
650 $atissue culture
650 $aFruta Tropical
650 $aMicropropagação
653 $aCultura de tecidos
653 $aGema axilar
653 $aNodal explant
653 $aPsidium guajava L
653 $aPsidium guava L
653 $aTropical fruit
700 1 $aJAISWAL, V. S.
773 $tPlant Cell, Tissue and Organ Culture$gv.9, p.235-243, 1987
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