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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
16/04/2008 |
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Data da última atualização: |
17/01/2024 |
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Autoria: |
MATOS, M. H. T.; HURK, R. van den; MARTINS, F. S.; SANTOS, R. R.; LUQUE, M. C. A.; SILVA, J. R. V.; CELESTINO, J. J. H.; BÁO, S. N.; FIGUEIREDO, J. R. |
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Título: |
Histological and ulrastructural features of aprine preantral follicles after in vitro culture in the presence or absence of indole-3-acetic acid. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
Animal Reproduction, v. 3, n. 4, p. 415-422, Oct./Dec. 2006. |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of the present study was to evaluate the effects of indole-3-acetic acid (IAA) on survival, activation, and growth of caprine preantral follicles using histological and ultrastructural criteria. Pieces of caprine ovarian cortex were cultured for 1 or 5 days in Minimum Essential Medium (MEM - control medium) supplemented with different concentrations of IAA (10, 20, 40, or 100 ng/ml). Small fragments from non-cultured ovarian tissue and from those cultured for 1 or 5 days in a specific medium were processed for classical histology and ultrastructural analysis. Follicle and oocyte growth were evaluated before and after 1 or 5 days of culture in the various media tested. The results showed a greater percentage of histologically-normal follicles in MEM alone or MEM supplemented with IAA (20 ng/ml) than other treatments. Indole-3-acetic acid added at a concentration of 20 or 40 ng/ml increased the proportion of primordial follicles that entered the growth phase after 5 days. In the presence of 10 or 20 ng/ml IAA, the follicles increased in diameter after 5 days of culture. Ultrastructural studies, however, did not confirm maintenance of the morphological integrity of caprine follicles cultured for 1 or 5 days in MEM supplemented with 20 ng/ml of IAA as was demonstrated by histological studies but in contrast showed distinct degenerative changes in both oocytes and granulosa cells. In conclusion, ultrastructural integrity of caprine preantral follicles can be successfully maintained after in vitro culture for 5 days in MEM without addition of IAA. In addition, ultrastructural analysis is necessary to judge the morphology of cultured preantral follicles since IAA negatively affects the ultrastructural composition of in vitro activated follicles, which was not detected by classical histology. MenosThe aim of the present study was to evaluate the effects of indole-3-acetic acid (IAA) on survival, activation, and growth of caprine preantral follicles using histological and ultrastructural criteria. Pieces of caprine ovarian cortex were cultured for 1 or 5 days in Minimum Essential Medium (MEM - control medium) supplemented with different concentrations of IAA (10, 20, 40, or 100 ng/ml). Small fragments from non-cultured ovarian tissue and from those cultured for 1 or 5 days in a specific medium were processed for classical histology and ultrastructural analysis. Follicle and oocyte growth were evaluated before and after 1 or 5 days of culture in the various media tested. The results showed a greater percentage of histologically-normal follicles in MEM alone or MEM supplemented with IAA (20 ng/ml) than other treatments. Indole-3-acetic acid added at a concentration of 20 or 40 ng/ml increased the proportion of primordial follicles that entered the growth phase after 5 days. In the presence of 10 or 20 ng/ml IAA, the follicles increased in diameter after 5 days of culture. Ultrastructural studies, however, did not confirm maintenance of the morphological integrity of caprine follicles cultured for 1 or 5 days in MEM supplemented with 20 ng/ml of IAA as was demonstrated by histological studies but in contrast showed distinct degenerative changes in both oocytes and granulosa cells. In conclusion, ultrastructural integrity of caprine preantral follicles can be successfully ... Mostrar Tudo |
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Palavras-Chave: |
Ácido acético; Ácido-3-indol acético; Foliculo pre-antral; Oocito; Ultraestrutura. |
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Thesagro: |
Caprino; Reprodução. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02748naa a2200301 a 4500
001 1533908
005 2024-01-17
008 2006 bl uuuu u00u1 u #d
100 1 $aMATOS, M. H. T.
245 $aHistological and ulrastructural features of aprine preantral follicles after in vitro culture in the presence or absence of indole-3-acetic acid.$h[electronic resource]
260 $c2006
520 $aThe aim of the present study was to evaluate the effects of indole-3-acetic acid (IAA) on survival, activation, and growth of caprine preantral follicles using histological and ultrastructural criteria. Pieces of caprine ovarian cortex were cultured for 1 or 5 days in Minimum Essential Medium (MEM - control medium) supplemented with different concentrations of IAA (10, 20, 40, or 100 ng/ml). Small fragments from non-cultured ovarian tissue and from those cultured for 1 or 5 days in a specific medium were processed for classical histology and ultrastructural analysis. Follicle and oocyte growth were evaluated before and after 1 or 5 days of culture in the various media tested. The results showed a greater percentage of histologically-normal follicles in MEM alone or MEM supplemented with IAA (20 ng/ml) than other treatments. Indole-3-acetic acid added at a concentration of 20 or 40 ng/ml increased the proportion of primordial follicles that entered the growth phase after 5 days. In the presence of 10 or 20 ng/ml IAA, the follicles increased in diameter after 5 days of culture. Ultrastructural studies, however, did not confirm maintenance of the morphological integrity of caprine follicles cultured for 1 or 5 days in MEM supplemented with 20 ng/ml of IAA as was demonstrated by histological studies but in contrast showed distinct degenerative changes in both oocytes and granulosa cells. In conclusion, ultrastructural integrity of caprine preantral follicles can be successfully maintained after in vitro culture for 5 days in MEM without addition of IAA. In addition, ultrastructural analysis is necessary to judge the morphology of cultured preantral follicles since IAA negatively affects the ultrastructural composition of in vitro activated follicles, which was not detected by classical histology.
650 $aCaprino
650 $aReprodução
653 $aÁcido acético
653 $aÁcido-3-indol acético
653 $aFoliculo pre-antral
653 $aOocito
653 $aUltraestrutura
700 1 $aHURK, R. van den
700 1 $aMARTINS, F. S.
700 1 $aSANTOS, R. R.
700 1 $aLUQUE, M. C. A.
700 1 $aSILVA, J. R. V.
700 1 $aCELESTINO, J. J. H.
700 1 $aBÁO, S. N.
700 1 $aFIGUEIREDO, J. R.
773 $tAnimal Reproduction$gv. 3, n. 4, p. 415-422, Oct./Dec. 2006.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
25/09/2009 |
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Data da última atualização: |
08/06/2018 |
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Autoria: |
FALQUETO, A. R.; CASSOL, D.; MAGALHAES JUNIOR, A. M. de; OLIVEIRA, A. C. de; BACARIN, M. A. |
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Afiliação: |
Antelmo Ralph Falqueto, Universidade Federal do Espírito Santo/Departamento de Ciências da Saúde/Biológicas e Agrárias; Daniela Cassol, Universidade Federal de Pelotas - UFPel/Departamento de Botânica/Instituto de Biologia; ARIANO MARTINS DE MAGALHAES JUNIOR, CPACT; Antônio Costa de Oliveira, UFPel/Faculdade de Agronomia Eliseu Maciel/Departamento de Fitotecnia; Marcos Antonio Bacarin, Universidade Federal de Pelotas - UFPel/Departamento de Botânica/Instituto de Biologia. |
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Título: |
Physiological analysis of leaf senescence of two rice cultivars with different yield potential. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 44, n. 7, p. 695-700, jul. 2009. |
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Idioma: |
Inglês |
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Notas: |
Título em português: Análise fi siológica da senescência foliar de duas cultivares de arroz com diferentes potenciais de produtividade. |
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Conteúdo: |
The objective of this work was to evaluate the physiological changes that occur in different leaves during the early and late grain-filling stages of two rice genotypes (Oryza sativa subsp. indica, BRS Pelota cultivar, and O. sativa subsp. japonica, BRS Firmeza cultivar), which present differences in grain yield potential. The plants were cultivated in greenhouse. Pigment content, chlorophyll fluorescence, electron transport and oxygen evolution rate were determined in the grain-filling stage, from the first to the forth leaf (top to bottom). Pigment content, photochemical efficiency of photosystem II and electron transport decreased significantly according to the position of leaves in 'BRS Pelota'. The BRS Firmeza cultivar shows higher pigment content and higher activity of the photosynthetic apparatus in comparison to 'BRS Pelota' during the grain-filling stage. |
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Palavras-Chave: |
Eficiência fotoquímica; Extinção fotoquímica; Fotossistema II; Oxygen evolution rate; Photochemical quenching; Photochemicol efficiency; Potencial de produtividade; Senescência foliar de cultivares de arroz; Senescência retardada; Stay green; Taxa de evolução de oxigênio. |
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Thesagro: |
Oryza Sativa; Oxigênio; Senescência. |
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Thesaurus NAL: |
Photosystem II. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/AI-SEDE-2010/46927/1/44n07a07.pdf
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Marc: |
LEADER 02138naa a2200361 a 4500
001 1631523
005 2018-06-08
008 2009 bl uuuu u00u1 u #d
100 1 $aFALQUETO, A. R.
245 $aPhysiological analysis of leaf senescence of two rice cultivars with different yield potential.
260 $c2009
500 $aTítulo em português: Análise fi siológica da senescência foliar de duas cultivares de arroz com diferentes potenciais de produtividade.
520 $aThe objective of this work was to evaluate the physiological changes that occur in different leaves during the early and late grain-filling stages of two rice genotypes (Oryza sativa subsp. indica, BRS Pelota cultivar, and O. sativa subsp. japonica, BRS Firmeza cultivar), which present differences in grain yield potential. The plants were cultivated in greenhouse. Pigment content, chlorophyll fluorescence, electron transport and oxygen evolution rate were determined in the grain-filling stage, from the first to the forth leaf (top to bottom). Pigment content, photochemical efficiency of photosystem II and electron transport decreased significantly according to the position of leaves in 'BRS Pelota'. The BRS Firmeza cultivar shows higher pigment content and higher activity of the photosynthetic apparatus in comparison to 'BRS Pelota' during the grain-filling stage.
650 $aPhotosystem II
650 $aOryza Sativa
650 $aOxigênio
650 $aSenescência
653 $aEficiência fotoquímica
653 $aExtinção fotoquímica
653 $aFotossistema II
653 $aOxygen evolution rate
653 $aPhotochemical quenching
653 $aPhotochemicol efficiency
653 $aPotencial de produtividade
653 $aSenescência foliar de cultivares de arroz
653 $aSenescência retardada
653 $aStay green
653 $aTaxa de evolução de oxigênio
700 1 $aCASSOL, D.
700 1 $aMAGALHAES JUNIOR, A. M. de
700 1 $aOLIVEIRA, A. C. de
700 1 $aBACARIN, M. A.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 44, n. 7, p. 695-700, jul. 2009.
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