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 | Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Florestas. |
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Data corrente: |
13/11/2019 |
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Data da última atualização: |
13/03/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BETTENCOURT, G. M. de F.; SOCCOL, C. R.; GIOVANELLA, T. S.; FRANCISCON, L.; KESTRING, D. R.; GERHARDT, I. R.; DEGENHARDT-GOLDBACH, J. |
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Afiliação: |
Gisela Manuela de França Bettencourt, UFPR; Carlos Ricardo Soccol, UFPR; Thais Salete Giovanella, UFPR; LUZIANE FRANCISCON, CNPF; DAIANE RIGONI, CNPF; ISABEL RODRIGUES GERHARDT, CNPTIA; JULIANA DEGENHARDT GOLDBACH, CNPF. |
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Título: |
Agrobacterium tumefaciens-mediated transformation of Eucalyptus urophylla clone BRS07-01. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Journal of Forestry Research, v. 31, n. 2, p. 507-519, Apr. 2020. |
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DOI: |
10.1007/s11676-018-0777-4 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract Genetic transformation is becoming routine for engineering specific traits in important clones of recalcitrant species such as Eucalyptus; however, the efficiency is still low for most species, so many researchers still use seeds instead of clones as initial explants. This work aimed to develop a genetic transformation protocol, based on a highly efficient in vitro organogenesis protocol, for an Eucalyptus urophylla clone selected in our breeding program. Plant growth regulators were evaluated for indirect organogenesis and rooting. In a two-step protocol, the combination of callus induction media supplemented with 0.5 lM thidiazuron + 0.5 lM naphthaleneacetic acid (NAA) and shoot induction media supplemented with 5.0 lM benzylaminopurine + 1.0 lM NAA allowed up to 85.6% shoot formation with more shoots per explants when compared with other concentrations. Transgenic plants expressing the uidA gene were obtained using Agrobacterium tumefaciens and selected for kanamycin resistance. A RAPD analysis was used to check for somaclonal variation. In tests using 11 RAPD primers, we did not observe somaclonal variation in the in vitro stages evaluated. |
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Palavras-Chave: |
Plant growth regulators; Regeneração in vitro; Reguladores de crescimento de plantas; Transformação genética. |
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Thesagro: |
Eucalipto; Eucalyptus Urophylla. |
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Thesaurus Nal: |
Genetic transformation; In vitro regeneration; Somaclonal variation. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02181naa a2200313 a 4500
001 2114265
005 2020-03-13
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1007/s11676-018-0777-4$2DOI
100 1 $aBETTENCOURT, G. M. de F.
245 $aAgrobacterium tumefaciens-mediated transformation of Eucalyptus urophylla clone BRS07-01.$h[electronic resource]
260 $c2020
520 $aAbstract Genetic transformation is becoming routine for engineering specific traits in important clones of recalcitrant species such as Eucalyptus; however, the efficiency is still low for most species, so many researchers still use seeds instead of clones as initial explants. This work aimed to develop a genetic transformation protocol, based on a highly efficient in vitro organogenesis protocol, for an Eucalyptus urophylla clone selected in our breeding program. Plant growth regulators were evaluated for indirect organogenesis and rooting. In a two-step protocol, the combination of callus induction media supplemented with 0.5 lM thidiazuron + 0.5 lM naphthaleneacetic acid (NAA) and shoot induction media supplemented with 5.0 lM benzylaminopurine + 1.0 lM NAA allowed up to 85.6% shoot formation with more shoots per explants when compared with other concentrations. Transgenic plants expressing the uidA gene were obtained using Agrobacterium tumefaciens and selected for kanamycin resistance. A RAPD analysis was used to check for somaclonal variation. In tests using 11 RAPD primers, we did not observe somaclonal variation in the in vitro stages evaluated.
650 $aGenetic transformation
650 $aIn vitro regeneration
650 $aSomaclonal variation
650 $aEucalipto
650 $aEucalyptus Urophylla
653 $aPlant growth regulators
653 $aRegeneração in vitro
653 $aReguladores de crescimento de plantas
653 $aTransformação genética
700 1 $aSOCCOL, C. R.
700 1 $aGIOVANELLA, T. S.
700 1 $aFRANCISCON, L.
700 1 $aKESTRING, D. R.
700 1 $aGERHARDT, I. R.
700 1 $aDEGENHARDT-GOLDBACH, J.
773 $tJournal of Forestry Research$gv. 31, n. 2, p. 507-519, Apr. 2020.
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Embrapa Florestas (CNPF) |
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| 1. |  | AGLIO, M. D.; CARVALHO JUNIOR, W. de C.; OLIVEIRA, R. P. de; SOUZA, J. S. de; BONAN, V. Modelagem topografica de declividade para classificacao de solos: uma comparacao metodologica. In: CONGRESSO BRASILEIRO DE CIENCIA DO SOLO, 26., 1997, Rio de Janeiro, RJ. Resumos... Rio de Janeiro: Sociedade Brasileira de Ciencia do Solo, 1997. p.341.| Biblioteca(s): Embrapa Solos. |
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| 2. | | CARDOSO, E. L.; OLIVEIRA, H. de; AMARAL, J. A. M. do; KER, J. C.; PEREIRA, N. R.; SANTOS, R. D. dos; TOSTO, S. G.; SPERA, S. T.; CARVALHO JUNIOR, W. de C. Pedologia. In: SILVA, J. dos S.V. da, org. Zoneamento ambiental da Borda Oeste do Pantanal: macico do Urucum e adjacencias. Brasilia: Embrapa Comunicacao para Transferencia de Tecnologia, 2000. p.95-110.| Biblioteca(s): Embrapa Pantanal. |
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