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 | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Oriental. Para informações adicionais entre em contato com cpatu.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Oriental; Embrapa Gado de Leite. |
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Data corrente: |
14/12/2020 |
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Data da última atualização: |
14/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FIGUEIRO, M. R.; GARCIA, J. M.; LIMA, M. R. de; COLLADO, M. del; SARAIVA, N. Z. |
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Afiliação: |
MARIVALDO RODRIGUES FIGUEIRO, CPATU; Joaquim Mansano Garcia, UNESP; Marina Ragagnin de Lima, UNESP; Maite del Collado, USP; NAIARA ZOCCAL SARAIVA, CNPGL. |
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Título: |
Is it possible to alter the embryo lipid accumulation with reduction of fetal bovine serum and use of l-carnitine for in vitro maturation of bubaline oocytes? |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Zygote, v. 28, n. 2, p. 109-115, Apr. 2020. |
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DOI: |
https://doi.org/10.1017/S096719941900073X |
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Idioma: |
Inglês |
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Conteúdo: |
In vitro embryo production (IVEP) is a procedure that can promote genetic improvement in a short time frame. However, the success rates obtained with this biotechnology in water buffaloes are still inconsistent, and can be associated with the high concentration of lipids in the cytoplasm of oocytes and embryos. The objective of this study was to evaluate the effects of reduced concentration of fetal bovine serum (FBS) and/or use of l-carnitine during in vitro maturation (IVM) on the preimplantation development and lipid accumulation in bubaline embryos. In a first experiment, the lowest concentration of FBS in the IVM medium (0%, 2.5%, 5% or 10%) was determined, and the lowest concentration that maintained good embryo development rates was 5%. In a second experiment, the addition of 5 mM of l-carnitine into the maturation medium was evaluated. The blastocysts produced were submitted to lipid evaluation involving staining followed by observation using optical (Oil Red O) and confocal (BODIPY 493/503) microscopy. No difference was observed between the 5% and 10% FBS groups, which were superior to the 0% and 2.5% groups. Furthermore, the performance of the groups treated with 5% and 10% FBS was better than the groups supplemented with l-carnitine. There was no difference regarding embryo lipid accumulation. The results indicated that it is possible to reduce the FBS concentration to 5% in in vitro maturation medium for production of bubaline embryos, and supplementation with 5 mM l-carnitine does not increase embryo production. MenosIn vitro embryo production (IVEP) is a procedure that can promote genetic improvement in a short time frame. However, the success rates obtained with this biotechnology in water buffaloes are still inconsistent, and can be associated with the high concentration of lipids in the cytoplasm of oocytes and embryos. The objective of this study was to evaluate the effects of reduced concentration of fetal bovine serum (FBS) and/or use of l-carnitine during in vitro maturation (IVM) on the preimplantation development and lipid accumulation in bubaline embryos. In a first experiment, the lowest concentration of FBS in the IVM medium (0%, 2.5%, 5% or 10%) was determined, and the lowest concentration that maintained good embryo development rates was 5%. In a second experiment, the addition of 5 mM of l-carnitine into the maturation medium was evaluated. The blastocysts produced were submitted to lipid evaluation involving staining followed by observation using optical (Oil Red O) and confocal (BODIPY 493/503) microscopy. No difference was observed between the 5% and 10% FBS groups, which were superior to the 0% and 2.5% groups. Furthermore, the performance of the groups treated with 5% and 10% FBS was better than the groups supplemented with l-carnitine. There was no difference regarding embryo lipid accumulation. The results indicated that it is possible to reduce the FBS concentration to 5% in in vitro maturation medium for production of bubaline embryos, and supplementation with 5 ... Mostrar Tudo |
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Palavras-Chave: |
Desenvolvimento embrionário; Soro fetal bovino. |
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Thesagro: |
Búfalo; Lipídio. |
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Thesaurus Nal: |
Buffaloes; Lipid metabolism. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02374naa a2200253 a 4500
001 2128094
005 2020-12-14
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1017/S096719941900073X$2DOI
100 1 $aFIGUEIRO, M. R.
245 $aIs it possible to alter the embryo lipid accumulation with reduction of fetal bovine serum and use of l-carnitine for in vitro maturation of bubaline oocytes?$h[electronic resource]
260 $c2020
520 $aIn vitro embryo production (IVEP) is a procedure that can promote genetic improvement in a short time frame. However, the success rates obtained with this biotechnology in water buffaloes are still inconsistent, and can be associated with the high concentration of lipids in the cytoplasm of oocytes and embryos. The objective of this study was to evaluate the effects of reduced concentration of fetal bovine serum (FBS) and/or use of l-carnitine during in vitro maturation (IVM) on the preimplantation development and lipid accumulation in bubaline embryos. In a first experiment, the lowest concentration of FBS in the IVM medium (0%, 2.5%, 5% or 10%) was determined, and the lowest concentration that maintained good embryo development rates was 5%. In a second experiment, the addition of 5 mM of l-carnitine into the maturation medium was evaluated. The blastocysts produced were submitted to lipid evaluation involving staining followed by observation using optical (Oil Red O) and confocal (BODIPY 493/503) microscopy. No difference was observed between the 5% and 10% FBS groups, which were superior to the 0% and 2.5% groups. Furthermore, the performance of the groups treated with 5% and 10% FBS was better than the groups supplemented with l-carnitine. There was no difference regarding embryo lipid accumulation. The results indicated that it is possible to reduce the FBS concentration to 5% in in vitro maturation medium for production of bubaline embryos, and supplementation with 5 mM l-carnitine does not increase embryo production.
650 $aBuffaloes
650 $aLipid metabolism
650 $aBúfalo
650 $aLipídio
653 $aDesenvolvimento embrionário
653 $aSoro fetal bovino
700 1 $aGARCIA, J. M.
700 1 $aLIMA, M. R. de
700 1 $aCOLLADO, M. del
700 1 $aSARAIVA, N. Z.
773 $tZygote$gv. 28, n. 2, p. 109-115, Apr. 2020.
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Registro original: |
Embrapa Amazônia Oriental (CPATU) |
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| Registros recuperados : 38 | |
| 9. |  | ABREU, U. G. P. de; GOMES, E. G.; CARVALHO, T. B. de; MALAFAIA, G. C. Estimativa de eficiência e análise de fatores contextuais para caracterização da intensificação dos sistemas de produção de gado de corte, em Mato Grosso do Sul. In: JORNADA NESPro, 18.; SIMPÓSIO INTERNACIONAL SOBRE SISTEMAS DE PRODUÇÃO DE BOVINOS DE CORTE, 6., 2023, Porto Alegre. Anais... Porto Alegre: UFRGS, 2023.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte; Embrapa Pantanal; Embrapa Unidades Centrais. |
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| 11. | | ABREU, U. G. P. de; CARVALHO, T. B. de; MORAES, A. S. Preço do bezerro pago no Pantanal da Nhecolândia - MS, no período de 2001 a 2008. REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 46., 2009, Maringá, PR. Inovação científica e tecnológica em zootecnia: anais. Maringá, PR: SBZ, 2009. Disponível também no CD2009.00004.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Pantanal. |
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| 15. | | MELLO, J. C. S. de; GOMES, E. G.; CARVALHO, T. B. de; ABREU, U. G. P. de; ZEN, S. de. Análise de desempenho de alguns sistemas de produção modais de pecuária de cria do Brasil. SIMPÓSIO SOBRE RECURSOS NATURAIS E SOCIOECONÔMICOS DO PANTANAL, 5., 2010, Corumbá, MS. Anais... Corumbá: Embrapa Pantanal: UFMS; Campinas: ICS do Brasil, 2010. 1 CD-ROM SIMPAN 2010. Não Paginado.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Pantanal. |
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| 16. | | ABREU, U. G. P. de; CARVALHO, T. B. de; RIBEIRO, G. G.; ZEN, S. de; MORAES, A. S.; DOMINGOS, I. Production cost of organic beef cattle production system in Pantanal - Brazil. Landbauforschung, v. 362, p. 88-92, 2012. Publicado no: Proceedings of the 2nd Organic Animal Husbandry Conference, Hamburg, Trenthorst, 12-14 September, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Pantanal. |
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| 17. | | RESENDE, R. S.; NASCIMENTO, T.; CARVALHO, T. B. de; AMORIM, J. R. A. de; RODRIGUES, L. N. Reducing sugarcane irrigation demand through planting date adjustment in Alagoas State, Brazil. Revista Brasileira de Engenharia Agrícola e Ambiental, Campina Grande, v. 25, n. 2, p. 75-81, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Cerrados; Embrapa Tabuleiros Costeiros. |
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| 18. | | SANTOS, M. C. dos; ZEN, S. de; ABREU, U. G. P. de; CARVALHO, T. B. de; GUARDA, G. M. Os ganhos de escala dos sistemas modais de produção de pecuária de corte em Corumbá, MS. In: CONGRESSO DA SOCIEDADE BRASILEIRA DE ECONOMIA, ADMINISTRAÇÃO E SOCIOLOGIA RURAL, 53., 2015, João Pessoa. Agropecuária, meio ambiente e desenvolvimento: anais. João Pessoal: Sober, 2015.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Pantanal. |
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| 20. | | CRESPOLINI, M.; DE ZEN, S.; ABREU, U. G. P. de; CARVALHO, T. B. de; YANAGUIZAWA, W. H.; GUARDA, G. M. Pecuária de corte no Pantanal - análise temporal e de escala, do sistema modal de produção em Corumbá-MS. Corumbá, Embrapa Pantanal, 2017. 8 p. (Embrapa Pantanal. Comunicado Técnico, 103).| Tipo: Comunicado Técnico/Recomendações Técnicas |
| Biblioteca(s): Embrapa Pantanal. |
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| Registros recuperados : 38 | |
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