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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
29/08/2002 |
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Data da última atualização: |
07/06/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MOTA, F. F. da; NOBREGA, F. F.; MARRIEL, I. E.; PAIVA, E.; SELDIN, L. |
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Afiliação: |
IVANILDO EVODIO MARRIEL, CNPMS. |
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Título: |
Genetic diversity of Paenibacillus polymyxa populations isolated from the rhizosphere of four cultivars of maize (Zea mays) planted in cerrado soil. |
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Ano de publicação: |
2002 |
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Fonte/Imprenta: |
Applied Soil Ecology, Amsterdam, v. 20, n. 2, p. 119-132, 2002. |
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Idioma: |
Inglês |
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Conteúdo: |
A tropical Brazilian soil (Cerrado) was planted with four cultivars of maize (CMS04, CMS11, CMS22 and CMS36) and the genetic diversity of the Paenibacillus polymyxa populations present in their rhizospheres was determined after 90 days of sowing. For that, a total of 67 isolates were identified as R polymyxa by classical biochemical tests and were analyzed for DNA polymorphism with the randomly amplified polymorphic DNA (RAPD) and amplification of repetitive DNA sequences (rep) methods. The amplification patterns obtained using three arbitrary primers and the primer BOXA1R were used separately to construct dendrograms based on the unweighted pair groups method with arithmetic means (UPGMA). Fifty-four genotypic groups were formed when data from different PCR amplification were combined, showing a high level of genetic polymorphism among P. polymyxa strains. A dendrogram based also on combined PCR data, followed by cluster analysis with minimum-variance criteria (Ward) and Euclidean distance, showed that P. polymyxa strains could be divided into two main clusters. One cluster was formed predominantly by strains from maize cultivars CMS04 and CMS36, while the other cluster was formed predominantly by strains of maize cultivars CMS11 and CMS22. Multivariate analysis of variance (MANOVA) allowed the correlation between the genetic structure of P. polymyxa populations and the different cultivars of maize to be studied. The results showed that the strains isolated from the rhizospheres of the different maize cultivars were significantly different. (C) 2002 Elsevier Science B. V. Alls rights reserved. MenosA tropical Brazilian soil (Cerrado) was planted with four cultivars of maize (CMS04, CMS11, CMS22 and CMS36) and the genetic diversity of the Paenibacillus polymyxa populations present in their rhizospheres was determined after 90 days of sowing. For that, a total of 67 isolates were identified as R polymyxa by classical biochemical tests and were analyzed for DNA polymorphism with the randomly amplified polymorphic DNA (RAPD) and amplification of repetitive DNA sequences (rep) methods. The amplification patterns obtained using three arbitrary primers and the primer BOXA1R were used separately to construct dendrograms based on the unweighted pair groups method with arithmetic means (UPGMA). Fifty-four genotypic groups were formed when data from different PCR amplification were combined, showing a high level of genetic polymorphism among P. polymyxa strains. A dendrogram based also on combined PCR data, followed by cluster analysis with minimum-variance criteria (Ward) and Euclidean distance, showed that P. polymyxa strains could be divided into two main clusters. One cluster was formed predominantly by strains from maize cultivars CMS04 and CMS36, while the other cluster was formed predominantly by strains of maize cultivars CMS11 and CMS22. Multivariate analysis of variance (MANOVA) allowed the correlation between the genetic structure of P. polymyxa populations and the different cultivars of maize to be studied. The results showed that the strains isolated from the rhizosp... Mostrar Tudo |
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Palavras-Chave: |
Diversidade genetica. |
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Thesagro: |
Bactéria; Cerrado; Milho; Rizosfera. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/66483/1/Genetic-diversity-1.pdf
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Marc: |
LEADER 02330naa a2200229 a 4500
001 1485544
005 2018-06-07
008 2002 bl uuuu u00u1 u #d
100 1 $aMOTA, F. F. da
245 $aGenetic diversity of Paenibacillus polymyxa populations isolated from the rhizosphere of four cultivars of maize (Zea mays) planted in cerrado soil.$h[electronic resource]
260 $c2002
520 $aA tropical Brazilian soil (Cerrado) was planted with four cultivars of maize (CMS04, CMS11, CMS22 and CMS36) and the genetic diversity of the Paenibacillus polymyxa populations present in their rhizospheres was determined after 90 days of sowing. For that, a total of 67 isolates were identified as R polymyxa by classical biochemical tests and were analyzed for DNA polymorphism with the randomly amplified polymorphic DNA (RAPD) and amplification of repetitive DNA sequences (rep) methods. The amplification patterns obtained using three arbitrary primers and the primer BOXA1R were used separately to construct dendrograms based on the unweighted pair groups method with arithmetic means (UPGMA). Fifty-four genotypic groups were formed when data from different PCR amplification were combined, showing a high level of genetic polymorphism among P. polymyxa strains. A dendrogram based also on combined PCR data, followed by cluster analysis with minimum-variance criteria (Ward) and Euclidean distance, showed that P. polymyxa strains could be divided into two main clusters. One cluster was formed predominantly by strains from maize cultivars CMS04 and CMS36, while the other cluster was formed predominantly by strains of maize cultivars CMS11 and CMS22. Multivariate analysis of variance (MANOVA) allowed the correlation between the genetic structure of P. polymyxa populations and the different cultivars of maize to be studied. The results showed that the strains isolated from the rhizospheres of the different maize cultivars were significantly different. (C) 2002 Elsevier Science B. V. Alls rights reserved.
650 $aBactéria
650 $aCerrado
650 $aMilho
650 $aRizosfera
653 $aDiversidade genetica
700 1 $aNOBREGA, F. F.
700 1 $aMARRIEL, I. E.
700 1 $aPAIVA, E.
700 1 $aSELDIN, L.
773 $tApplied Soil Ecology, Amsterdam$gv. 20, n. 2, p. 119-132, 2002.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Biblioteca |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Hortaliças. Para informações adicionais entre em contato com cnph.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Hortaliças. |
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Data corrente: |
09/12/2008 |
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Data da última atualização: |
15/03/2010 |
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Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
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Autoria: |
SILVA NETO, I. G.; REIS, A.; CARVALHO, S. I. C.; OLIVEIRA, V. R.; REZENDE, W. M, LIMA, I. B.; COSTA, G. M.; BOITEUX, L. S. |
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Afiliação: |
Ailton Reis, Embrapa Hortaliças; Sabrina Isabel Costa de Carvalho, Embrapa Hortaliças; Valter Rodrigues de Oliveira, Embrapa Hortaliças; Leonardo Silva Boiteux, Embrapa Hortaliças; Isaias Gama da Silva Neto, Faculdade de Guaraí; Israel Barbosa Lima, Facu. |
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Título: |
Avaliação de germoplasma de melão (cucumis melo) para resistência ao Phytophthora capsici na fase de plântula. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Horticultura Brasileira, Brasília, DF, v. 26, n. 2, p. S4780-S4786, 2008. |
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Idioma: |
Português |
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Notas: |
Suplemento. CD-ROM. Trabalho apresentado no 48. Congresso Brasileiro de Oleiricultura, Maringá, 2008. |
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Palavras-Chave: |
Oomiceto. |
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Thesagro: |
Cucumis Melo; Germoplasma; Melão; Phytophthora Capsici; Plântula; Resistência. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00911naa a2200277 a 4500
001 1780893
005 2010-03-15
008 2008 bl uuuu u00u1 u #d
100 1 $aSILVA NETO, I. G.
245 $aAvaliação de germoplasma de melão (cucumis melo) para resistência ao Phytophthora capsici na fase de plântula.
260 $c2008
500 $aSuplemento. CD-ROM. Trabalho apresentado no 48. Congresso Brasileiro de Oleiricultura, Maringá, 2008.
650 $aCucumis Melo
650 $aGermoplasma
650 $aMelão
650 $aPhytophthora Capsici
650 $aPlântula
650 $aResistência
653 $aOomiceto
700 1 $aREIS, A.
700 1 $aCARVALHO, S. I. C.
700 1 $aOLIVEIRA, V. R.
700 1 $aREZENDE, W. M, LIMA, I. B.
700 1 $aCOSTA, G. M.
700 1 $aBOITEUX, L. S.
773 $tHorticultura Brasileira, Brasília, DF$gv. 26, n. 2, p. S4780-S4786, 2008.
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