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Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos; Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
04/10/2011 |
Data da última atualização: |
16/02/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
PAIVA, S. R.; FACO, O.; FARIA, D. A.; LACERDA, T.; BARRETO, G. B. B.; CARNEIRO, P. L. S.; LOBO, R. N. B. |
Afiliação: |
SAMUEL REZENDE PAIVA, CENARGEN; OLIVARDO FACO, CNPC; DANIELLE A. FARIA; THAÍSA LACERDA; GABRIEL B. BARRETO, Faculdades Integradas, UPIS, Brasília, DF.; PAULO L. S. CARNEIRO, Universidade Estadual do Sudoeste da Bahia, Jequié, BA.; RAIMUNDO NONATO BRAGA LOBO, CNPC. |
Título: |
Molecular and pedigree analysis applied to conservation of animal genetic resources: the case of Brazilian Somali hair sheep. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
Tropical Animal Health and Production, v. 43, n. 7, p. 1449-1457, Oct., 2011. |
DOI: |
10.1007/s11250-011-9873-6 |
Idioma: |
Inglês |
Conteúdo: |
The first registers of Somali sheep in Brazil are from the beginning of the 1900s. This breed, adapted to the dry climate and scarce food supply, is restricted in the northeast region of the country. Molecular marker technolo-gies, especially those based on genotyping microsatellite and mtDNA loci, can be used in conjunction with breeding (pedigree analysis) and consequently the maintenance of genetic variation in herds. Animals from the Brazilian Somali Conservation Nuclei from Embrapa Sheep and Goats in Ceará State were used to validate genetic monitoring by traditional pedigree methods and molecular markers. Nineteen micro-satellite markers and 404 base pairs from the control region of mtDNA were used. For total herd diversity, an average 5.32 alleles were found, with expected heterozygosity of 0.5896, observed heterozygosity of 0.6451, 0.4126 for molecular coancestrality, and coefficient of inbreeding (FIS)was ?0.095. Comparing molecular coancestrality means over the years, there was a consistent increase in this parameter within the herd, increasing from 0.4157 to 0.4769 in 2 years (approx. 12% variation). Sixteen mtDNA haplotypes were identified. Inbreeding and other estimates from genealogical analyses confirm the results from molecular markers. From these results, it is possible to state that microsatellites are useful tools in genetic management of herds, especially when routine herd recording is not carried out, or there were gaps in recent generations. As well as pedigree control, genetic diversity can be optimized. Based on the results, and despite herd recording in the herd of Brazilian Somali of Embrapa Sheep and Goats, additional management measures need to be carried out in this herd to reduce inbreeding and optimize genetic variation. MenosThe first registers of Somali sheep in Brazil are from the beginning of the 1900s. This breed, adapted to the dry climate and scarce food supply, is restricted in the northeast region of the country. Molecular marker technolo-gies, especially those based on genotyping microsatellite and mtDNA loci, can be used in conjunction with breeding (pedigree analysis) and consequently the maintenance of genetic variation in herds. Animals from the Brazilian Somali Conservation Nuclei from Embrapa Sheep and Goats in Ceará State were used to validate genetic monitoring by traditional pedigree methods and molecular markers. Nineteen micro-satellite markers and 404 base pairs from the control region of mtDNA were used. For total herd diversity, an average 5.32 alleles were found, with expected heterozygosity of 0.5896, observed heterozygosity of 0.6451, 0.4126 for molecular coancestrality, and coefficient of inbreeding (FIS)was ?0.095. Comparing molecular coancestrality means over the years, there was a consistent increase in this parameter within the herd, increasing from 0.4157 to 0.4769 in 2 years (approx. 12% variation). Sixteen mtDNA haplotypes were identified. Inbreeding and other estimates from genealogical analyses confirm the results from molecular markers. From these results, it is possible to state that microsatellites are useful tools in genetic management of herds, especially when routine herd recording is not carried out, or there were gaps in recent generations. As well as ... Mostrar Tudo |
Palavras-Chave: |
Ceará; D-loop; Genetic management; Microsatelite; Molecular markers; Raça Somalis Brasileira; Recurso genético animal. |
Thesagro: |
Endogamia; Genética animal; Manejo; Marcador molecular; Ovino; Variação genética. |
Thesaurus Nal: |
Animal genetics; Brazil; Sheep. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/42786/1/API-Molecular.pdf
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Marc: |
LEADER 02939naa a2200397 a 4500 001 1902648 005 2023-02-16 008 2011 bl uuuu u00u1 u #d 024 7 $a10.1007/s11250-011-9873-6$2DOI 100 1 $aPAIVA, S. R. 245 $aMolecular and pedigree analysis applied to conservation of animal genetic resources$bthe case of Brazilian Somali hair sheep.$h[electronic resource] 260 $c2011 520 $aThe first registers of Somali sheep in Brazil are from the beginning of the 1900s. This breed, adapted to the dry climate and scarce food supply, is restricted in the northeast region of the country. Molecular marker technolo-gies, especially those based on genotyping microsatellite and mtDNA loci, can be used in conjunction with breeding (pedigree analysis) and consequently the maintenance of genetic variation in herds. Animals from the Brazilian Somali Conservation Nuclei from Embrapa Sheep and Goats in Ceará State were used to validate genetic monitoring by traditional pedigree methods and molecular markers. Nineteen micro-satellite markers and 404 base pairs from the control region of mtDNA were used. For total herd diversity, an average 5.32 alleles were found, with expected heterozygosity of 0.5896, observed heterozygosity of 0.6451, 0.4126 for molecular coancestrality, and coefficient of inbreeding (FIS)was ?0.095. Comparing molecular coancestrality means over the years, there was a consistent increase in this parameter within the herd, increasing from 0.4157 to 0.4769 in 2 years (approx. 12% variation). Sixteen mtDNA haplotypes were identified. Inbreeding and other estimates from genealogical analyses confirm the results from molecular markers. From these results, it is possible to state that microsatellites are useful tools in genetic management of herds, especially when routine herd recording is not carried out, or there were gaps in recent generations. As well as pedigree control, genetic diversity can be optimized. Based on the results, and despite herd recording in the herd of Brazilian Somali of Embrapa Sheep and Goats, additional management measures need to be carried out in this herd to reduce inbreeding and optimize genetic variation. 650 $aAnimal genetics 650 $aBrazil 650 $aSheep 650 $aEndogamia 650 $aGenética animal 650 $aManejo 650 $aMarcador molecular 650 $aOvino 650 $aVariação genética 653 $aCeará 653 $aD-loop 653 $aGenetic management 653 $aMicrosatelite 653 $aMolecular markers 653 $aRaça Somalis Brasileira 653 $aRecurso genético animal 700 1 $aFACO, O. 700 1 $aFARIA, D. A. 700 1 $aLACERDA, T. 700 1 $aBARRETO, G. B. B. 700 1 $aCARNEIRO, P. L. S. 700 1 $aLOBO, R. N. B. 773 $tTropical Animal Health and Production$gv. 43, n. 7, p. 1449-1457, Oct., 2011.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registro Completo
Biblioteca(s): |
Embrapa Agroindústria de Alimentos; Embrapa Amazônia Oriental. |
Data corrente: |
26/02/2009 |
Data da última atualização: |
13/02/2014 |
Tipo da produção científica: |
Documentos |
Autoria: |
FREITAS, S. C. de; SILVA, T. dos S.; CARVALHO, P. G. B. de; TUPINAMBA, D. D.; KOAKUZU, S. N.; CARVALHO, A. V.; MOURA, C. F. H. |
Afiliação: |
SIDINÉA CORDEIRO DE FREITAS, CTAA; TANIA DOS SANTOS SILVA, CTAA; PATRÍCIA GONÇALVES BAPTISTA DE CARVALHO, CNPH; DAIVA DOMENECH TUPINAMBÁ, CPAC; SELMA NAKAMOTO KOAKUZU, CNPAF; ANA VÂNIA CARVALHO, CPATU; CARLOS FARLEY HERBSTER MOURA, CNPAT. |
Título: |
Procedimento operacional padrão para determinação de fibras solúvel e insolúvel. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
Rio de Janeiro: Embrapa Agroindústria de Alimentos, 2008. |
Páginas: |
28 p. |
Série: |
(Embrapa Agroindústria de Alimentos. Documentos, 94). |
ISSN: |
0103-6068 |
Idioma: |
Português |
Palavras-Chave: |
Fibra insolúvel; Fibra solúvel. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/97331/1/2008-DOC-0094-2.pdf
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Marc: |
LEADER 00737nam a2200229 a 4500 001 1416714 005 2014-02-13 008 2008 bl uuuu u0uu1 u #d 022 $a0103-6068 100 1 $aFREITAS, S. C. de 245 $aProcedimento operacional padrão para determinação de fibras solúvel e insolúvel.$h[electronic resource] 260 $aRio de Janeiro: Embrapa Agroindústria de Alimentos$c2008 300 $a28 p. 490 $a(Embrapa Agroindústria de Alimentos. Documentos, 94). 653 $aFibra insolúvel 653 $aFibra solúvel 700 1 $aSILVA, T. dos S. 700 1 $aCARVALHO, P. G. B. de 700 1 $aTUPINAMBA, D. D. 700 1 $aKOAKUZU, S. N. 700 1 $aCARVALHO, A. V. 700 1 $aMOURA, C. F. H.
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Embrapa Agroindústria de Alimentos (CTAA) |
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