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| Registros recuperados : 12 | |
| 5. |  | GUIMARÃES, C. C.; ROSA, S. D. V. F. da; MALTA, M. R.; CARVALHO, M. H.; OLIVEIRA, R. M. E. Lipid content and fatty acid profile in Embryos, endosperm, and seeds of dried Coffea arabica. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 26., 2016, Kunming,Yunnan, China. Proceedings... ASIC - Association for Science and Information on Coffee, 2016. p. 120.| Biblioteca(s): Embrapa Café. |
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| 7. | | BALDONI, A. B.; ARAÚJO, A. C. G.; CARVALHO, M. H.; DALL'ASTTA, R. C.; ARAGÃO, F. J. L. Castor bean (Ricinus communis L.) seed development. In: SIMPÓSIO DE MICROSCOPIA NO CERRADO, 2008, Pirenópolis, GO. [Anais...] Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. 321 p. (Embrapa Recursos Genéticos e Biotecnologia, 265) Organizadoras: Ana Claudia Guerra de Araújo e Sônia Nair Báo. p. 48-50.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 9. | | BALDONI, A. B.; CARVALHO, M. H. DE.; SOUSA, N. L.; NOBREGA, M. B. de M.; MILANI, M.; ARAGÃO, F. J. L. Variability of ricin content in mature seeds of castor bean. Pesquisa Agropecuária Brasileira, v. 46, n. 7, p. 776-779, jul., 2011.| Biblioteca(s): Embrapa Algodão; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Unidades Centrais. |
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| 10. | | CARVALHO, M. H. de; ROSA, S. D. V. F. da; COELHO, S. V. B.; GUIMARÃES, C. C.; MARTINS, R. de S.; CLEMENTE, A. da C. S.; PAIVA, L. V. Drying of arabica coffee and its effect on the gene expression and activity of enzymes linked to seed physiological quality. Acta Scientiarum: Agronomy, v. 45, e56908, 2023.| Biblioteca(s): Embrapa Café. |
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| 11. | | CADEIRA, L. A.; ALVES, E. E.; RIBEIRO, A. de M. F.; ROCHA JÚNIOR, V. R.; ANTUNES, A. B.; REIS, A. F. dos; GOMES, J. da C.; CARVALHO, M. H. R. de; ESPINOSA MARTINEZ, R. I. Viability of probiotic bacteria in bioyogurt with the addition of honey from Jataí and Africanized bees. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 53, n. 2, p. 206-211, Fev. 2018. Título em português: Viabilidade de bactérias probióticas em bioiogurte adicionado de mel de abelhas Jataí e africanizadas.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 12. | | RIBEIRO, T. P.; BASSO, M. F.; CARVALHO, M. H. de; MACEDO, L. L. P. de; SILVA, D. M. L. da; LOURENCO-TESSUTTI, I. T.; OLIVEIRA-NETO, O. B. de; CAMPOS-PINTO, E. R. de; LUCENA, W. A.; SILVA, M. C. M. da; TRIPODE, B. M. D.; ABREU-JARDIM, T. P. F.; MIRANDA, J. E.; ALVES-FERREIRA, M.; MORGANTE, C. V.; GROSSI-DE-SA, M. F. Stability and tissue-specific Cry10Aa overexpression improves cotton resistance to the cotton boll weevil. Biotechnology Research and Innovation, v. 3, p. 27-41, 2020.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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| Registros recuperados : 12 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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Data corrente: |
01/09/2020 |
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Data da última atualização: |
19/11/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 5 |
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Autoria: |
RIBEIRO, T. P.; BASSO, M. F.; CARVALHO, M. H. de; MACEDO, L. L. P. de; SILVA, D. M. L. da; LOURENCO-TESSUTTI, I. T.; OLIVEIRA-NETO, O. B. de; CAMPOS-PINTO, E. R. de; LUCENA, W. A.; SILVA, M. C. M. da; TRIPODE, B. M. D.; ABREU-JARDIM, T. P. F.; MIRANDA, J. E.; ALVES-FERREIRA, M.; MORGANTE, C. V.; GROSSI-DE-SA, M. F. |
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Afiliação: |
THUANNE PIRES RIBEIRO, UNB; MARCOS FERNANDO BASSO; MAYARA HOLANDA DE CARVALHO; LEONARDO LIMA PEPINO DE MACEDO, Cenargen; DAGNA MARIA LAURINDO DA SILVA; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; OSMUNDO BRILANTE DE OLIVEIRA-NETO; EDUARDO ROMANO DE CAMPOS PINTO, Cenargen; WAGNER ALEXANDRE LUCENA, Cenargen; MARIA CRISTINA MATTAR DA SILVA, Cenargen; BRUNA MENDES DINIZ TRIPODE, CNPA; TATIANNE PIZA FERRARI ABREU-JARDIM; JOSE EDNILSON MIRANDA, CNPA; MARCIO ALVES-FERREIRA, UFRJ; CAROLINA VIANNA MORGANTE, CPATSA; MARIA FATIMA GROSSI DE SA, Cenargen. |
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Título: |
Stability and tissue-specific Cry10Aa overexpression improves cotton resistance to the cotton boll weevil. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Biotechnology Research and Innovation, v. 3, p. 27-41, 2020. |
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DOI: |
https://doi.org/10.1016/j.biori.2019.12.003 |
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Idioma: |
Inglês |
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Conteúdo: |
The cotton boll weevil (CBW, Anthonomus grandis) is the most destructive cotton insect pest affecting cotton crops. To overcome this problem, CBW-resistant genetically modified cotton plants overexpressing Bacillus thuringiensis entomotoxins were successfully obtained. Previous results showed that the overexpression of Cry10Aa protoxin led to high mortality of the CBW larvae in greenhouse conditions. In this study, we advanced three more generations (T2 to T4), with several cotton events constitutively overexpressing the Cry10Aa protoxin, and the transgene stability and agronomic performance were investigated. In addition, stable transgenic cotton overexpressing the Cry10Aa active (Cry10Aa protoxin lacking the -helix N-terminal) driven by cotton flower bud-specific promoters were generated and characterized. Cotton events constitutively or tissue-specifically overexpressing the Cry10Aa protein (protoxin or active) represented mortality percentages of the CBW larva of up to 85 % in plants under greenhouse conditions. Events overexpressing the Cry10Aa active under control of the flower bud-specific promoter showed higher protein accumulation in stamens and carpels compared to the events with constitutive expression. Our findings suggested that the high stability of the Cry10Aa transgene and the elevated expression level and protein accumulation in flower bud tissues (primarily in stamen and carpels) contribute to improved resistance to CBW larvae. Finally, some notable events were selected with potential for future field trials in different cotton- producing regions of Brazil. Therefore, cotton events overexpressing high levels of the Cry10Aa protein in flower bud tissue may have a strong potential for commercial use in the integrated management of CBW. MenosThe cotton boll weevil (CBW, Anthonomus grandis) is the most destructive cotton insect pest affecting cotton crops. To overcome this problem, CBW-resistant genetically modified cotton plants overexpressing Bacillus thuringiensis entomotoxins were successfully obtained. Previous results showed that the overexpression of Cry10Aa protoxin led to high mortality of the CBW larvae in greenhouse conditions. In this study, we advanced three more generations (T2 to T4), with several cotton events constitutively overexpressing the Cry10Aa protoxin, and the transgene stability and agronomic performance were investigated. In addition, stable transgenic cotton overexpressing the Cry10Aa active (Cry10Aa protoxin lacking the -helix N-terminal) driven by cotton flower bud-specific promoters were generated and characterized. Cotton events constitutively or tissue-specifically overexpressing the Cry10Aa protein (protoxin or active) represented mortality percentages of the CBW larva of up to 85 % in plants under greenhouse conditions. Events overexpressing the Cry10Aa active under control of the flower bud-specific promoter showed higher protein accumulation in stamens and carpels compared to the events with constitutive expression. Our findings suggested that the high stability of the Cry10Aa transgene and the elevated expression level and protein accumulation in flower bud tissues (primarily in stamen and carpels) contribute to improved resistance to CBW larvae. Finally, some notable events ... Mostrar Tudo |
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Palavras-Chave: |
Bacillus; Bicudo do algodão; Entomotoxin; Herança estável; Insect pest; Management; Praga de inseto; Resistance; Resistência gestão; Stable inheritance; Thuringiensis. |
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Thesagro: |
Algodão; Inseto; Praga; Praga de Planta. |
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Thesaurus NAL: |
Cotton; Insect pests; Resistance management. |
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Categoria do assunto: |
--
O Insetos e Entomologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/216823/1/Stability-and-tissue-2020.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/215669/1/1-s2.0-S2452072119301352-main.pdf
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Marc: |
LEADER 03317naa a2200529 a 4500
001 2125667
005 2020-11-19
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.biori.2019.12.003$2DOI
100 1 $aRIBEIRO, T. P.
245 $aStability and tissue-specific Cry10Aa overexpression improves cotton resistance to the cotton boll weevil.$h[electronic resource]
260 $c2020
520 $aThe cotton boll weevil (CBW, Anthonomus grandis) is the most destructive cotton insect pest affecting cotton crops. To overcome this problem, CBW-resistant genetically modified cotton plants overexpressing Bacillus thuringiensis entomotoxins were successfully obtained. Previous results showed that the overexpression of Cry10Aa protoxin led to high mortality of the CBW larvae in greenhouse conditions. In this study, we advanced three more generations (T2 to T4), with several cotton events constitutively overexpressing the Cry10Aa protoxin, and the transgene stability and agronomic performance were investigated. In addition, stable transgenic cotton overexpressing the Cry10Aa active (Cry10Aa protoxin lacking the -helix N-terminal) driven by cotton flower bud-specific promoters were generated and characterized. Cotton events constitutively or tissue-specifically overexpressing the Cry10Aa protein (protoxin or active) represented mortality percentages of the CBW larva of up to 85 % in plants under greenhouse conditions. Events overexpressing the Cry10Aa active under control of the flower bud-specific promoter showed higher protein accumulation in stamens and carpels compared to the events with constitutive expression. Our findings suggested that the high stability of the Cry10Aa transgene and the elevated expression level and protein accumulation in flower bud tissues (primarily in stamen and carpels) contribute to improved resistance to CBW larvae. Finally, some notable events were selected with potential for future field trials in different cotton- producing regions of Brazil. Therefore, cotton events overexpressing high levels of the Cry10Aa protein in flower bud tissue may have a strong potential for commercial use in the integrated management of CBW.
650 $aCotton
650 $aInsect pests
650 $aResistance management
650 $aAlgodão
650 $aInseto
650 $aPraga
650 $aPraga de Planta
653 $aBacillus
653 $aBicudo do algodão
653 $aEntomotoxin
653 $aHerança estável
653 $aInsect pest
653 $aManagement
653 $aPraga de inseto
653 $aResistance
653 $aResistência gestão
653 $aStable inheritance
653 $aThuringiensis
700 1 $aBASSO, M. F.
700 1 $aCARVALHO, M. H. de
700 1 $aMACEDO, L. L. P. de
700 1 $aSILVA, D. M. L. da
700 1 $aLOURENCO-TESSUTTI, I. T.
700 1 $aOLIVEIRA-NETO, O. B. de
700 1 $aCAMPOS-PINTO, E. R. de
700 1 $aLUCENA, W. A.
700 1 $aSILVA, M. C. M. da
700 1 $aTRIPODE, B. M. D.
700 1 $aABREU-JARDIM, T. P. F.
700 1 $aMIRANDA, J. E.
700 1 $aALVES-FERREIRA, M.
700 1 $aMORGANTE, C. V.
700 1 $aGROSSI-DE-SA, M. F.
773 $tBiotechnology Research and Innovation$gv. 3, p. 27-41, 2020.
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Embrapa Semiárido (CPATSA) |
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