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| Registros recuperados : 16 | |
| 1. |  | LOPES, V. S.; CARVALHO, M. C. C. G. de; DIAS, W. P.; MARCELINO-GUIMARÃES, F. C. Genes Hsp20 de soja: organização no genoma e categorização entre subfamílias. In: CONGRESSO BRASILEIRO DE SOJA, 6., 2012, Cuiabá. Soja: integração nacional e desenvolvimento sustentável: resumos. Brasília, DF: Embrapa, 2012. p. 17, res. 12.| Biblioteca(s): Embrapa Soja. |
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| 4. | | DARBEN, L. M.; QI, M.; YOKOYAMA, A.; CARVALHO, M. C. C. G. de; ABDELNOOR, R. V.; WHITHAM, S. A.; MARCELINO-GUIMARÃES, F. C. Functional characterization of P. pachyrhizi effector candidates. In: INTERNATIONAL CONGRESS OF PLANT MOLECULAR BIOLOGY, 11., 2015, Iguassu Falls. Abstract... Abstract: 840.pdf.| Biblioteca(s): Embrapa Soja. |
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| 5. | | CARVALHO, K. de; RINCÃO, M. P.; DARBEN, L. M.; CARVALHO, M. C. C. G. de; LOPES, I. de O. N.; ABDELNOOR, R. V.; MARCELINO-GUIMARÃES, F. C. External application of dsRNA targeting a Phakopsora pachyrhizi effector candidate attenuated fungal pathogenicity. In: INTERNATIONAL PLANT & ANIMAL GENOME CONFERENCE, 25., 2017, San Diego. Abstracts... [S. l.: s. n.], 2017. não paginado. PLANT AND ANIMAL GENOME XXV CONFERENCE - INTLPAG, 2017.| Biblioteca(s): Embrapa Soja. |
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| 6. | | UTIYAMA, A. S.; ABE, V. Y.; DARBEN, L. M.; CARVALHO, K.; CARVALHO, M. C. C. G. de; MARCELINO-GUIMARÃES, F. C. Avaliação funcional de proteínas candidatas a efetores de Phakopsora pachyrhizi por meio da análise de espécies reativas a oxigênio (ROS) e deposição de calose. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 13., 2018, Londrina. Resumos expandidos... Londrina: Embrapa Soja, 2018. p. 38-45.| Biblioteca(s): Embrapa Soja. |
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| 7. | | POLIZEL-PODANOSQUI, A. M.; CARVALHO, M. C. C. G. de; ROCHA, C. S.; DARBEN, L. M.; LOPES-CAITAR, V. S.; AOYAOGI, L. N.; MARCELINO-GUIMARÃES, F. C. Temporal gene expression analysis of effector candidates of Phakopsora pachyrhizi across the infection cycle. In: INTERNATIONAL CONGRESS ON MOLECULAR PLANT-MICROBE INTERACTIONS, 16., 2014, Rhodes. [Abstracts...]. Atenas: International Society of Molecular Plant-Microbe Interactions, 2014.| Biblioteca(s): Embrapa Soja. |
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| 8. | | YOKOYAMA, A.; DARBEN, L. M.; LOPES-CAITAR, V. S.; AOYAGI, L. N.; CARVALHO, M. C. C. G. de; SOARES, R. M.; ALMEIDA, A. M. R.; MARCELINO-GUIMARÃES, F. C. Diversidade genética de isolados monouredinias de Phakopsora pachyrhizi coletados em diferentes regiões do Brasil. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 8., 2013, Londrina. Resumos expandidos... Londrina: Embrapa Soja, 2013. p. 11-15. (Embrapa Soja. Documentos, 339).| Biblioteca(s): Embrapa Soja. |
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| 9. | | LOPES-CAITAR, V. S.; CARVALHO, M. C. C. G. de; LUANA M. DARBEN; KUWAHARA, M. K.; NEPOMUCENO, A. L.; DIAS, W. P.; ABDELNOOR, R. V.; MARCELINO-GUIMARÃES, F. C. Genome-wide analysis of the Hsp20 gene family in soybean: comprehensive sequence, genomic organization and expression profile analysis under abiotic and biotic stresses. BMC Genomics, v. 14, article 577, 2013. 17 p.| Biblioteca(s): Embrapa Soja. |
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| 10. | | KUMA, K. M.; LOPES-CAITAR, V. S.; ROMERO, C. C. T.; DARBEN, L. M.; SILVA, S. M. H.; CARVALHO, M. C. C. G. de; KUWAHARA, M. K.; OLIVEIRA, M. C. N. de; DIAS, W. P.; MARCELINO-GUIMARÃES, F. C. Functional characterization of soybean GmHsp17.6B and GmHsp22.4 genes in response to Meloidogyne javanica infection. In: WORKSHOP ON BIOTIC AND ABIOTIC STRESS TOLERANCE IN PLANTS, 2013, Ilhéus. The challenge for the 21st century: book of abstracts. [Brasília]: Embrapa: International Advanced Biology Consortium, 2013. p. 34.| Biblioteca(s): Embrapa Soja. |
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| 11. | | AOYAGI, L. N.; LOPES-CAITAR, V. S.; CARVALHO, M. C. C. G. de; DARBEN, L. M.; POLIZEL-PODANOSQUI, A.; KUWAHARA, M. K.; NEPOMUCENO, A. L.; ABDELNOOR, R. V.; MARCELINO-GUIMARÃES, F. C. Genomic and transcriptomic characterization of the transcription factor family R2R3-MYB in soybean and its involvement in the resistance responses to Phakopsora pachyrhizi. Plant Sciense, Limerick, v. 229, p. 32-42, Dec. 2014.| Biblioteca(s): Embrapa Soja. |
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| 12. | | DARBEN, L. M.; YOKOYAMA, A.; PARMEZAN, T. R.; KUWAHARA, M. K.; CARVALHO, M. C. C. G. de; GONELA, A.; SOARES, R. M.; ALMEIDA, A. M. R.; GODOY, C. V.; MARCELINO-GUIMARÃES, F. C. Evaluation of virulence of Phakopsora pachyrhizi monourediniais isolates collected in Brazil. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 47.; SIMPÓSIO BRASILEIRO DE MOFO BRANCO, 2014, Londrina. Desafios futuros: anais. Londrina: SBF, 2014. 1 CD-ROM.| Biblioteca(s): Embrapa Soja. |
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| 13. | | DARBEN, L. M.; YOKOYAMA, A.; PARMEZAN, T. R.; LOPES-CAITAR, V. S.; AOYAGI, L. N.; CARVALHO, M. C. C. G. de; GODOY, C. V.; GONELA, A.; SOARES, R. M.; ALMEIDA, A. M. R.; MARCELINO-GUIMARÃES, F. C. Molecular diversity of monouredinial Phakopsora pachyrhizi isolates collected in Brazil. In: WORKSHOP ON BIOTIC AND ABIOTIC STRESS TOLERANCE IN PLANTS, 2013, Ilhéus. The challenge for the 21st century: book of abstracts. [Brasília]: Embrapa: International Advanced Biology Consortium, 2013. p. 56.| Biblioteca(s): Embrapa Soja. |
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| 14. | | CARVALHO, M. C. C. G. de; NASCIMENTO, L. C.; POLIZEL-PODANOSQUI, A. M.; ROCHA, C. S.; DARBEN, L. M.; LOPES-CAITAR, V. S.; CARAZZOLLE, M. F.; OLIVEIRA, M. L. C. S.; ABDELNOOR, R. V.; MARCELINO-GUIMARÃES, F. C. The transcriptome interactions between Phakopsora pachykopsora pachyrhizi-soybean. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 47.; SIMPÓSIO BRASILEIRO DE MOFO BRANCO, 2014, Londrina. Desafios futuros: anais. Londrina: SBF, 2014. 1 CD-ROM.| Biblioteca(s): Embrapa Soja. |
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| 15. | | BENCKE-MALATO, M.; CABREIRA, C.; WIEBKE-STROHM, B.; BÜCKER-NETO, L.; MANCINI, E.; OSORIO, M. B.; HOMRICH, M. S.; TURCHETTO-ZOLET, A. C.; CARVALHO, M. C. C. G. de; STOLF, R.; WEBER, R. L. M.; WESTERGAARD, G.; CASTAGNARO, A. P.; ABDELNOOR, R. V.; MARCELINO-GUIMARÃES, F. C.; MARGIS-PINHEIRO, M.; BODANESE-ZANETTINI, M. H. Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infection. BMC Plant Biology, v. 14, n. 1, article 236, Sept. 2014. 18 p.| Biblioteca(s): Embrapa Soja. |
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| 16. | | WIEBKE-STROHM, B.; PASQUALI, G.; MARGIS-PINHEIRO, M.; BENCKE, M.; BÜCKER-NETO, L.; BECKER-RITT, A. B.; MARTINELLI, A. H. S.; RECHENMACHER, C.; POLACCO, J. C.; STOLF, R.; MARCELINO, F. C.; ABDELNOOR, R. V.; HOMRICH, M. S.; DEL PONTE, E. M.; CARLINI, C. R.; CARVALHO, M. C. C. G. de; BODANESE-ZANETTINI, M. H. Ubiquitous urease affects soybean susceptibility to fungi. Plant Molecular Biology, The Hague, v. 79, n. 1/2, p. 75-87, May 2012.| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 16 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
05/03/2012 |
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Data da última atualização: |
19/01/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
WIEBKE-STROHM, B.; PASQUALI, G.; MARGIS-PINHEIRO, M.; BENCKE, M.; BÜCKER-NETO, L.; BECKER-RITT, A. B.; MARTINELLI, A. H. S.; RECHENMACHER, C.; POLACCO, J. C.; STOLF, R.; MARCELINO, F. C.; ABDELNOOR, R. V.; HOMRICH, M. S.; DEL PONTE, E. M.; CARLINI, C. R.; CARVALHO, M. C. C. G. de; BODANESE-ZANETTINI, M. H. |
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Afiliação: |
BEATRIZ WIEBKE-STROHM, UFRGS; GIANCARLO PASQUALI, UFRGS; MÁRCIA MARGIS-PINHEIRO, UFRGS; MARTA BENCKE, UFRGS; LAURO BÜCKER NETO, UFRGS; ARLETE B. BECKER-RITT, UFRGS; ANNE H. S. MARTINELLI, UFRGS; CILIANA RECHENMACHER, UFRGS; JOSEPH C. POLACCO, University of Missouri; RENATA STOLF, CNPSo; FRANCISMAR CORREA MARCELINO, CNPSO; RICARDO VILELA ABDELNOOR, CNPSO; MILENA S. HOMRICH, UFRGS; EMERSON M. DEL PONTE, UFRGS; CELIA R. CARLINI, UFRGS; MAYRA C. C. G. DE CARVALHO, CNPSo; MARIA HELENA BODANESE-ZANETTINI, UFRGS. |
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Título: |
Ubiquitous urease affects soybean susceptibility to fungi. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Plant Molecular Biology, The Hague, v. 79, n. 1/2, p. 75-87, May 2012. |
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DOI: |
DOI 10.1007/s11103-012-9894-1 |
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Idioma: |
Inglês |
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Conteúdo: |
The soybean ubiquitous urease (encoded by GmEu4) is responsible for recycling metabolically derived urea. Additional biological roles have been demonstrated for plant ureases, notably in toxicity to other organisms. However, urease enzymatic activity is not related to its toxicity. The role of GmEu4 in soybean susceptibility to fungi was investigated in this study. A differential expression pattern of GmEu4 was observed in susceptible and resistant genotypes of soybeans over the course of a Phakopsora pachyrhizi infection, especially 24 h after infection. Twenty-nine adult, transgenic soybean plants, representing six independently transformed lines, were obtained. Although the initial aim of this study was to overexpress GmEu4, the transgenic plants exhibited GmEu4 co-suppression and decreased ureolytic activity. The growth of Rhizoctonia solani, Phomopsis sp., and Penicillium herguei in media containing a crude protein extract from either transgenic or non-transgenic leaves was evaluated. The fungal growth was higher in the protein extracts from transgenic urease-deprived plants than in extracts from nontransgenic controls. When infected by P. pachyrhizi uredospores, detached leaves of urease-deprived plants developed a significantly higher number of lesions, pustules and erupted pustules than leaves of non-transgenic plants containing normal levels of the enzyme. The results of the present work show that the soybean plants were more susceptible to fungi in the absence of urease. It was not possible to overexpress active GmEu4. For future work, overexpression of urease fungitoxic peptides could be attempted as an alternative approach. MenosThe soybean ubiquitous urease (encoded by GmEu4) is responsible for recycling metabolically derived urea. Additional biological roles have been demonstrated for plant ureases, notably in toxicity to other organisms. However, urease enzymatic activity is not related to its toxicity. The role of GmEu4 in soybean susceptibility to fungi was investigated in this study. A differential expression pattern of GmEu4 was observed in susceptible and resistant genotypes of soybeans over the course of a Phakopsora pachyrhizi infection, especially 24 h after infection. Twenty-nine adult, transgenic soybean plants, representing six independently transformed lines, were obtained. Although the initial aim of this study was to overexpress GmEu4, the transgenic plants exhibited GmEu4 co-suppression and decreased ureolytic activity. The growth of Rhizoctonia solani, Phomopsis sp., and Penicillium herguei in media containing a crude protein extract from either transgenic or non-transgenic leaves was evaluated. The fungal growth was higher in the protein extracts from transgenic urease-deprived plants than in extracts from nontransgenic controls. When infected by P. pachyrhizi uredospores, detached leaves of urease-deprived plants developed a significantly higher number of lesions, pustules and erupted pustules than leaves of non-transgenic plants containing normal levels of the enzyme. The results of the present work show that the soybean plants were more susceptible to fungi in the absence of u... Mostrar Tudo |
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Palavras-Chave: |
Penicillium herguei; Transformação genética. |
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Thesagro: |
Fungo; Phomopsis; Queima da saia; Soja. |
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Thesaurus NAL: |
Genetic transformation; Soybeans. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
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024 7 $aDOI 10.1007/s11103-012-9894-1$2DOI
100 1 $aWIEBKE-STROHM, B.
245 $aUbiquitous urease affects soybean susceptibility to fungi.
260 $c2012
520 $aThe soybean ubiquitous urease (encoded by GmEu4) is responsible for recycling metabolically derived urea. Additional biological roles have been demonstrated for plant ureases, notably in toxicity to other organisms. However, urease enzymatic activity is not related to its toxicity. The role of GmEu4 in soybean susceptibility to fungi was investigated in this study. A differential expression pattern of GmEu4 was observed in susceptible and resistant genotypes of soybeans over the course of a Phakopsora pachyrhizi infection, especially 24 h after infection. Twenty-nine adult, transgenic soybean plants, representing six independently transformed lines, were obtained. Although the initial aim of this study was to overexpress GmEu4, the transgenic plants exhibited GmEu4 co-suppression and decreased ureolytic activity. The growth of Rhizoctonia solani, Phomopsis sp., and Penicillium herguei in media containing a crude protein extract from either transgenic or non-transgenic leaves was evaluated. The fungal growth was higher in the protein extracts from transgenic urease-deprived plants than in extracts from nontransgenic controls. When infected by P. pachyrhizi uredospores, detached leaves of urease-deprived plants developed a significantly higher number of lesions, pustules and erupted pustules than leaves of non-transgenic plants containing normal levels of the enzyme. The results of the present work show that the soybean plants were more susceptible to fungi in the absence of urease. It was not possible to overexpress active GmEu4. For future work, overexpression of urease fungitoxic peptides could be attempted as an alternative approach.
650 $aGenetic transformation
650 $aSoybeans
650 $aFungo
650 $aPhomopsis
650 $aQueima da saia
650 $aSoja
653 $aPenicillium herguei
653 $aTransformação genética
700 1 $aPASQUALI, G.
700 1 $aMARGIS-PINHEIRO, M.
700 1 $aBENCKE, M.
700 1 $aBÜCKER-NETO, L.
700 1 $aBECKER-RITT, A. B.
700 1 $aMARTINELLI, A. H. S.
700 1 $aRECHENMACHER, C.
700 1 $aPOLACCO, J. C.
700 1 $aSTOLF, R.
700 1 $aMARCELINO, F. C.
700 1 $aABDELNOOR, R. V.
700 1 $aHOMRICH, M. S.
700 1 $aDEL PONTE, E. M.
700 1 $aCARLINI, C. R.
700 1 $aCARVALHO, M. C. C. G. de
700 1 $aBODANESE-ZANETTINI, M. H.
773 $tPlant Molecular Biology, The Hague$gv. 79, n. 1/2, p. 75-87, May 2012.
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