| |
|
|
| Registros recuperados : 16 | |
| 1. |  | LOPES, V. S.; CARVALHO, M. C. C. G. de; DIAS, W. P.; MARCELINO-GUIMARÃES, F. C. Genes Hsp20 de soja: organização no genoma e categorização entre subfamílias. In: CONGRESSO BRASILEIRO DE SOJA, 6., 2012, Cuiabá. Soja: integração nacional e desenvolvimento sustentável: resumos. Brasília, DF: Embrapa, 2012. p. 17, res. 12.| Biblioteca(s): Embrapa Soja. |
|   |
| 4. | | DARBEN, L. M.; QI, M.; YOKOYAMA, A.; CARVALHO, M. C. C. G. de; ABDELNOOR, R. V.; WHITHAM, S. A.; MARCELINO-GUIMARÃES, F. C. Functional characterization of P. pachyrhizi effector candidates. In: INTERNATIONAL CONGRESS OF PLANT MOLECULAR BIOLOGY, 11., 2015, Iguassu Falls. Abstract... Abstract: 840.pdf.| Biblioteca(s): Embrapa Soja. |
|  |
| 5. | | CARVALHO, K. de; RINCÃO, M. P.; DARBEN, L. M.; CARVALHO, M. C. C. G. de; LOPES, I. de O. N.; ABDELNOOR, R. V.; MARCELINO-GUIMARÃES, F. C. External application of dsRNA targeting a Phakopsora pachyrhizi effector candidate attenuated fungal pathogenicity. In: INTERNATIONAL PLANT & ANIMAL GENOME CONFERENCE, 25., 2017, San Diego. Abstracts... [S. l.: s. n.], 2017. não paginado. PLANT AND ANIMAL GENOME XXV CONFERENCE - INTLPAG, 2017.| Biblioteca(s): Embrapa Soja. |
|  |
| 6. | | UTIYAMA, A. S.; ABE, V. Y.; DARBEN, L. M.; CARVALHO, K.; CARVALHO, M. C. C. G. de; MARCELINO-GUIMARÃES, F. C. Avaliação funcional de proteínas candidatas a efetores de Phakopsora pachyrhizi por meio da análise de espécies reativas a oxigênio (ROS) e deposição de calose. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 13., 2018, Londrina. Resumos expandidos... Londrina: Embrapa Soja, 2018. p. 38-45.| Biblioteca(s): Embrapa Soja. |
| |
| 7. | | POLIZEL-PODANOSQUI, A. M.; CARVALHO, M. C. C. G. de; ROCHA, C. S.; DARBEN, L. M.; LOPES-CAITAR, V. S.; AOYAOGI, L. N.; MARCELINO-GUIMARÃES, F. C. Temporal gene expression analysis of effector candidates of Phakopsora pachyrhizi across the infection cycle. In: INTERNATIONAL CONGRESS ON MOLECULAR PLANT-MICROBE INTERACTIONS, 16., 2014, Rhodes. [Abstracts...]. Atenas: International Society of Molecular Plant-Microbe Interactions, 2014.| Biblioteca(s): Embrapa Soja. |
| |
| 8. | | YOKOYAMA, A.; DARBEN, L. M.; LOPES-CAITAR, V. S.; AOYAGI, L. N.; CARVALHO, M. C. C. G. de; SOARES, R. M.; ALMEIDA, A. M. R.; MARCELINO-GUIMARÃES, F. C. Diversidade genética de isolados monouredinias de Phakopsora pachyrhizi coletados em diferentes regiões do Brasil. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 8., 2013, Londrina. Resumos expandidos... Londrina: Embrapa Soja, 2013. p. 11-15. (Embrapa Soja. Documentos, 339).| Biblioteca(s): Embrapa Soja. |
| |
| 9. | | LOPES-CAITAR, V. S.; CARVALHO, M. C. C. G. de; LUANA M. DARBEN; KUWAHARA, M. K.; NEPOMUCENO, A. L.; DIAS, W. P.; ABDELNOOR, R. V.; MARCELINO-GUIMARÃES, F. C. Genome-wide analysis of the Hsp20 gene family in soybean: comprehensive sequence, genomic organization and expression profile analysis under abiotic and biotic stresses. BMC Genomics, v. 14, article 577, 2013. 17 p.| Biblioteca(s): Embrapa Soja. |
| |
| 10. | | KUMA, K. M.; LOPES-CAITAR, V. S.; ROMERO, C. C. T.; DARBEN, L. M.; SILVA, S. M. H.; CARVALHO, M. C. C. G. de; KUWAHARA, M. K.; OLIVEIRA, M. C. N. de; DIAS, W. P.; MARCELINO-GUIMARÃES, F. C. Functional characterization of soybean GmHsp17.6B and GmHsp22.4 genes in response to Meloidogyne javanica infection. In: WORKSHOP ON BIOTIC AND ABIOTIC STRESS TOLERANCE IN PLANTS, 2013, Ilhéus. The challenge for the 21st century: book of abstracts. [Brasília]: Embrapa: International Advanced Biology Consortium, 2013. p. 34.| Biblioteca(s): Embrapa Soja. |
| |
| 11. | | AOYAGI, L. N.; LOPES-CAITAR, V. S.; CARVALHO, M. C. C. G. de; DARBEN, L. M.; POLIZEL-PODANOSQUI, A.; KUWAHARA, M. K.; NEPOMUCENO, A. L.; ABDELNOOR, R. V.; MARCELINO-GUIMARÃES, F. C. Genomic and transcriptomic characterization of the transcription factor family R2R3-MYB in soybean and its involvement in the resistance responses to Phakopsora pachyrhizi. Plant Sciense, Limerick, v. 229, p. 32-42, Dec. 2014.| Biblioteca(s): Embrapa Soja. |
| |
| 12. | | DARBEN, L. M.; YOKOYAMA, A.; PARMEZAN, T. R.; KUWAHARA, M. K.; CARVALHO, M. C. C. G. de; GONELA, A.; SOARES, R. M.; ALMEIDA, A. M. R.; GODOY, C. V.; MARCELINO-GUIMARÃES, F. C. Evaluation of virulence of Phakopsora pachyrhizi monourediniais isolates collected in Brazil. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 47.; SIMPÓSIO BRASILEIRO DE MOFO BRANCO, 2014, Londrina. Desafios futuros: anais. Londrina: SBF, 2014. 1 CD-ROM.| Biblioteca(s): Embrapa Soja. |
|  |
| 13. | | DARBEN, L. M.; YOKOYAMA, A.; PARMEZAN, T. R.; LOPES-CAITAR, V. S.; AOYAGI, L. N.; CARVALHO, M. C. C. G. de; GODOY, C. V.; GONELA, A.; SOARES, R. M.; ALMEIDA, A. M. R.; MARCELINO-GUIMARÃES, F. C. Molecular diversity of monouredinial Phakopsora pachyrhizi isolates collected in Brazil. In: WORKSHOP ON BIOTIC AND ABIOTIC STRESS TOLERANCE IN PLANTS, 2013, Ilhéus. The challenge for the 21st century: book of abstracts. [Brasília]: Embrapa: International Advanced Biology Consortium, 2013. p. 56.| Biblioteca(s): Embrapa Soja. |
| |
| 14. | | CARVALHO, M. C. C. G. de; NASCIMENTO, L. C.; POLIZEL-PODANOSQUI, A. M.; ROCHA, C. S.; DARBEN, L. M.; LOPES-CAITAR, V. S.; CARAZZOLLE, M. F.; OLIVEIRA, M. L. C. S.; ABDELNOOR, R. V.; MARCELINO-GUIMARÃES, F. C. The transcriptome interactions between Phakopsora pachykopsora pachyrhizi-soybean. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 47.; SIMPÓSIO BRASILEIRO DE MOFO BRANCO, 2014, Londrina. Desafios futuros: anais. Londrina: SBF, 2014. 1 CD-ROM.| Biblioteca(s): Embrapa Soja. |
| |
| 15. | | BENCKE-MALATO, M.; CABREIRA, C.; WIEBKE-STROHM, B.; BÜCKER-NETO, L.; MANCINI, E.; OSORIO, M. B.; HOMRICH, M. S.; TURCHETTO-ZOLET, A. C.; CARVALHO, M. C. C. G. de; STOLF, R.; WEBER, R. L. M.; WESTERGAARD, G.; CASTAGNARO, A. P.; ABDELNOOR, R. V.; MARCELINO-GUIMARÃES, F. C.; MARGIS-PINHEIRO, M.; BODANESE-ZANETTINI, M. H. Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infection. BMC Plant Biology, v. 14, n. 1, article 236, Sept. 2014. 18 p.| Biblioteca(s): Embrapa Soja. |
| |
| 16. | | WIEBKE-STROHM, B.; PASQUALI, G.; MARGIS-PINHEIRO, M.; BENCKE, M.; BÜCKER-NETO, L.; BECKER-RITT, A. B.; MARTINELLI, A. H. S.; RECHENMACHER, C.; POLACCO, J. C.; STOLF, R.; MARCELINO, F. C.; ABDELNOOR, R. V.; HOMRICH, M. S.; DEL PONTE, E. M.; CARLINI, C. R.; CARVALHO, M. C. C. G. de; BODANESE-ZANETTINI, M. H. Ubiquitous urease affects soybean susceptibility to fungi. Plant Molecular Biology, The Hague, v. 79, n. 1/2, p. 75-87, May 2012.| Biblioteca(s): Embrapa Soja. |
| |
| Registros recuperados : 16 | |
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Soja. |
|
Data corrente: |
29/12/2014 |
|
Data da última atualização: |
30/12/2014 |
|
Tipo da produção científica: |
Resumo em Anais de Congresso |
|
Autoria: |
POLIZEL-PODANOSQUI, A. M.; CARVALHO, M. C. C. G. de; ROCHA, C. S.; DARBEN, L. M.; LOPES-CAITAR, V. S.; AOYAOGI, L. N.; MARCELINO-GUIMARÃES, F. C. |
|
Afiliação: |
CNPSO - estagiário; UENP; CNPSO - estagiário; UENP; CNPSO - estagiário; CNPSO - estagiário; FRANCISMAR CORREA MARCELINO-GUIMARÃES, CNPSO. |
|
Título: |
Temporal gene expression analysis of effector candidates of Phakopsora pachyrhizi across the infection cycle. |
|
Ano de publicação: |
2014 |
|
Fonte/Imprenta: |
In: INTERNATIONAL CONGRESS ON MOLECULAR PLANT-MICROBE INTERACTIONS, 16., 2014, Rhodes. [Abstracts...]. Atenas: International Society of Molecular Plant-Microbe Interactions, 2014. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Phakopsora pachyrhizi is the causer of the Asian soybean rust, the most aggressive foliar disease that attacks the soybean plants in cultivated areas. To design effective breeding strategies for durable disease resistance it is important to understand the molecular basis of this plant-pathogen interaction. In particular, the characterization of the secreted effector proteins can help the breeding programs. Our recent analysis of the P. pachyrhizi secretome has revealed the presence of potential effectors genes. In this work, the temporal expression of 58 effector candidate genes were characterized from spores, germinated spores and infected soybean leaves at 0, 6, 12, 24, 36, 48, 72, 96, 192 and 240 hours after inoculation (hai) using RT-qPCR. Expression analysis showed differential temporal gene expression of these candidate effectors. Clustering analysis revealed three main clusters with coordinated expression of candidates across the infection cycle. The first has candidates expressed in specific steps of infection and almost sequences have the common motifs Y/F/WxC frequently found in haustoria-producing rust fungi effectors. The second cluster has candidates expressed during initial steps of infection that matches germ tube and appressorium formation (0 – 24 hai) and almost sequences have ?3 cysteine residues, other common features of the effectors. Third cluster has candidates expressed only in contact with the host, suggesting that these candidates are host-induced. The sequences in this cluster have the CFEM domain associated with pathogenicity. Functional analyses are in progress with more promising candidates effectors to validate the potential effectors from soybean rust. MenosPhakopsora pachyrhizi is the causer of the Asian soybean rust, the most aggressive foliar disease that attacks the soybean plants in cultivated areas. To design effective breeding strategies for durable disease resistance it is important to understand the molecular basis of this plant-pathogen interaction. In particular, the characterization of the secreted effector proteins can help the breeding programs. Our recent analysis of the P. pachyrhizi secretome has revealed the presence of potential effectors genes. In this work, the temporal expression of 58 effector candidate genes were characterized from spores, germinated spores and infected soybean leaves at 0, 6, 12, 24, 36, 48, 72, 96, 192 and 240 hours after inoculation (hai) using RT-qPCR. Expression analysis showed differential temporal gene expression of these candidate effectors. Clustering analysis revealed three main clusters with coordinated expression of candidates across the infection cycle. The first has candidates expressed in specific steps of infection and almost sequences have the common motifs Y/F/WxC frequently found in haustoria-producing rust fungi effectors. The second cluster has candidates expressed during initial steps of infection that matches germ tube and appressorium formation (0 – 24 hai) and almost sequences have ?3 cysteine residues, other common features of the effectors. Third cluster has candidates expressed only in contact with the host, suggesting that these candidates are host-induced. T... Mostrar Tudo |
|
Palavras-Chave: |
Ferrugem asiática da soja. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/114606/1/Temporal-gene-expression-analysis-of-effector-candidates-of-Phakopsora-pachyrhizi-across-the-infection-cycle.pdf
|
|
Marc: |
LEADER 02492nam a2200193 a 4500
001 2003884
005 2014-12-30
008 2014 bl uuuu u00u1 u #d
100 1 $aPOLIZEL-PODANOSQUI, A. M.
245 $aTemporal gene expression analysis of effector candidates of Phakopsora pachyrhizi across the infection cycle.$h[electronic resource]
260 $aIn: INTERNATIONAL CONGRESS ON MOLECULAR PLANT-MICROBE INTERACTIONS, 16., 2014, Rhodes. [Abstracts...]. Atenas: International Society of Molecular Plant-Microbe Interactions$c2014
520 $aPhakopsora pachyrhizi is the causer of the Asian soybean rust, the most aggressive foliar disease that attacks the soybean plants in cultivated areas. To design effective breeding strategies for durable disease resistance it is important to understand the molecular basis of this plant-pathogen interaction. In particular, the characterization of the secreted effector proteins can help the breeding programs. Our recent analysis of the P. pachyrhizi secretome has revealed the presence of potential effectors genes. In this work, the temporal expression of 58 effector candidate genes were characterized from spores, germinated spores and infected soybean leaves at 0, 6, 12, 24, 36, 48, 72, 96, 192 and 240 hours after inoculation (hai) using RT-qPCR. Expression analysis showed differential temporal gene expression of these candidate effectors. Clustering analysis revealed three main clusters with coordinated expression of candidates across the infection cycle. The first has candidates expressed in specific steps of infection and almost sequences have the common motifs Y/F/WxC frequently found in haustoria-producing rust fungi effectors. The second cluster has candidates expressed during initial steps of infection that matches germ tube and appressorium formation (0 – 24 hai) and almost sequences have ?3 cysteine residues, other common features of the effectors. Third cluster has candidates expressed only in contact with the host, suggesting that these candidates are host-induced. The sequences in this cluster have the CFEM domain associated with pathogenicity. Functional analyses are in progress with more promising candidates effectors to validate the potential effectors from soybean rust.
653 $aFerrugem asiática da soja
700 1 $aCARVALHO, M. C. C. G. de
700 1 $aROCHA, C. S.
700 1 $aDARBEN, L. M.
700 1 $aLOPES-CAITAR, V. S.
700 1 $aAOYAOGI, L. N.
700 1 $aMARCELINO-GUIMARÃES, F. C.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Soja (CNPSO) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
