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| Registros recuperados : 76 | |
| 23. |  | FAGERIA, N. K.; GHEYI, H. R.; CARVALHO, M. C. S.; MOREIRA, A. Root growth, nutrient uptake, and nutrient-use efficiency by roots of tropical legume cover crops as influenced by phosphorus fertilization. In: INOVAGRI INTERNATIONAL MEETING, 2., 2014, Fortaleza. Anais... Fortaleza: Instituto de Pesquisa e Inovação na Agricultura Irrigada, 2014. 1 CD-ROM.| Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Soja. |
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| 26. | | LOPES, V. S.; CARVALHO, M. C. C. G. de; DIAS, W. P.; MARCELINO-GUIMARÃES, F. C. Genes Hsp20 de soja: organização no genoma e categorização entre subfamílias. In: CONGRESSO BRASILEIRO DE SOJA, 6., 2012, Cuiabá. Soja: integração nacional e desenvolvimento sustentável: resumos. Brasília, DF: Embrapa, 2012. p. 17, res. 12.| Biblioteca(s): Embrapa Soja. |
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| 33. | | SOSA-GOMEZ, D. R.; CARVALHO, M. C. da C. G. de; MARCELINO-GUIMARÃES, F. C.; HOFFMANN-CAMPO, C. B. A biotecnologia, o melhoramento e o manejo de pragas da soja. In: HOFFMANN-CAMPO, C. B.; CORRÊA-FERREIRA, B. S.; MOSCARDI, F. Soja: manejo integrado de insetos e outros Artrópodes-praga. Brasília, DF: Embrapa, 2012. p. 725-788.| Biblioteca(s): Embrapa Soja. |
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| 34. | | HAMADA, E.; ANGELOTTI, F.; GARRIDO, L. da R.; GHINI, R.; CARVALHO, M. C. de; PALLADINO, R. P. Efeito das mudanças climáticas sobre a favorabilidade às podridões da uva madura e cinzenta da videira no Nordeste brasileiro. In: SIMPÓSIO DE MUDANÇAS CLIMÁTICAS E DESERTIFICAÇÃO NO SEMIÁRIDO BRASILEIRO, 3., 2011, Juazeiro. Experiências para mitigação e adaptação. Petrolina: Embrapa Semiárido, 2011. (Embrapa Semiárido. Documentos, 239).| Biblioteca(s): Embrapa Meio Ambiente; Embrapa Semiárido. |
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| 35. | | HOLANDA, J. S.; BRASIL, E. C.; SALVIANO, A. A. C.; CARVALHO, M. C. S.; RODRIGUES, M. R. L.; MALAVOLTA, E. Eficiência de extratores de fósforo num solo adubado com fosfatos e cultivado com arroz. IN: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 21. Petrolina, PE, 1994. Anais... Petrolina: EMBRAPA-CPATSA; SBCS, 1994. p. 46-48| Biblioteca(s): Embrapa Amazônia Ocidental; Embrapa Tabuleiros Costeiros. |
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| 36. | | HOLANDA, J. S.; BRASIL, E. C.; SALVIANO, A. A. C.; CARVALHO, M. C. S.; RODRIGUES, M. R. L.; MALAVOLTA, E. Eficiência de extratores de fósforo num solo adubado com fosfatos e cultivado com arroz. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 21., 1994, Petrolina. Anais... Petrolina: EMBRAPA-CPATSA: SBCS, 1994. p. 46-48.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 37. | | SANTOS, F. C. dos; ALBUQUERQUE FILHO, M. R. de; BARBOSA, C. A. da S.; FERREIRA, G. B.; CARVALHO, M. C. S.; BREDA, C. E. Manejo da adubação nitrogenada e crescimento do algodoeiro cultivado no Cerrado da Bahia sob irrigação. In: SIMPÓSIO NACIONAL CERRADO, 9.; SIMPÓSIO INTERNACIONAL SAVANAS TROPICAIS, 2., 2008, Brasília, DF. Desafios e estratégias para o equilíbrio entre sociedade, agronegócio e recursos naturais: anais... Planaltina, DF: Embrapa Cerrados, 2008. 1 CD-ROM.| Biblioteca(s): Embrapa Cerrados. |
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| 38. | | DARDENGO, L.; ROQUE, C. G.; SILVANETO, J. A.; SILVA, C. J.; CARVALHO, M. C.; LANGE, A.; VIOTTO, R. F. V.; MONTEIRO, S. Atributos físicos do solo em sistemas de semeadura de arroz de sequeiro na Região Norte Matogrossense In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 28.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 12.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 10.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 7., 2008, Londrina. FertBio 2008: desafios para o uso do solo com eficiência e qualidade ambiental: resumos. Londrina: Embrapa Soja: SBCS: IAPAR: UEL, 2008. Organizado por: Adilson de Oliveira Júnior, Regina Maria Villas Bôas de Campos Leite, César de Castro, Fábio Álvares de Oliveira; Odilon Ferreira Saraiva. p. 150. Resumo n. 781.| Biblioteca(s): Embrapa Agropecuária Oeste. |
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| 40. | | CAITAR, V. S. L.; NASCIMENTO, L. C.; CARVALHO, M. C. D. C. G. D.; DARBEN, L. M; PODANOSQUI, A. M. P.; DIAS, W. P.; GUIMARÃES, F. C. M. Earlier transcriptome interaction from resistant and susceptible soybean genotypes under Pratylenchus brachyurus infection revealed by RNA-seq. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 46.; REUNIÃO BRASILEIRA DE CONTROLE BIOLÓGICO, 11., 2013, Ouro Preto. [Anais...]. [Brasília]: Sociedade Brasileira de Fitopatologia, 2013. 1 CD-ROM.| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 76 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
17/12/2015 |
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Data da última atualização: |
05/08/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
KUMA, K. M.; LOPES-CAITAR, V. S.; ROMERO, C. C. T.; SILVA, S. M. H.; CARVALHO, M. C. C. G.; ABDELNOOR, R. V.; DIAS, W. P.; MARCELINO-GUIMARÃES, F. C. |
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Afiliação: |
UEL; UEL; CNPSo; UEL; UENP; RICARDO VILELA ABDELNOOR, CNPSO; WALDIR PEREIRA DIAS, CNPSO; FRANCISMAR CORREA MARCELINO GUIMARÃES, CNPSO. |
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Título: |
A high efficient protocol for soybean root transformation by Agrobacterium rhizogenes and most stable reference genes for RT-qPCR analysis. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Plant Cell Reports, v. 34, n. 11, p. 1987-2000, 2015. |
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ISSN: |
1432-203X |
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DOI: |
10.1007/s00299-015-1845-2 |
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Idioma: |
Inglês |
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Conteúdo: |
Gene functional analyses are essential to the validation of results obtained from in silico and/or geneprospecting studies. Genetic transformation methods that yield tissues of transient expression quickly have been of considerable interest to researchers. Agrobacterium rhizogenes-mediated transformation methods, which are employed to generate plants with transformed roots, have proven useful for the study of stress caused by root phytopathogens via gene overexpression and/or silencing. While some protocols have been adapted to soybean plants, transformation efficiencies remain limited; thus, few viable plants are available for performing bioassays. Furthermore, mRNA analyses that employ reverse transcription quantitative polymerase chain reactions (RT-qPCR) require the use of reference genes with stable expression levels across different organs, development steps and treatments. In the present study, an A. rhizogenes-mediated soybean root transformation approach was optimized. The method delivers significantly higher transformation efficiency levels and rates of transformed plant recovery, thus enhancing studies of soybean abiotic conditions or interactions between phytopathogens, such as nematodes. A 55 % transformation efficiency was obtained following the addition of an acclimation step that involves hydroponics and different selection processes. The present study also validated the reference genes GmELF1-b and GmELF1-a as the most stable to be used in RT-qPCR analysis in composite plants, mainly under nematode infection. MenosGene functional analyses are essential to the validation of results obtained from in silico and/or geneprospecting studies. Genetic transformation methods that yield tissues of transient expression quickly have been of considerable interest to researchers. Agrobacterium rhizogenes-mediated transformation methods, which are employed to generate plants with transformed roots, have proven useful for the study of stress caused by root phytopathogens via gene overexpression and/or silencing. While some protocols have been adapted to soybean plants, transformation efficiencies remain limited; thus, few viable plants are available for performing bioassays. Furthermore, mRNA analyses that employ reverse transcription quantitative polymerase chain reactions (RT-qPCR) require the use of reference genes with stable expression levels across different organs, development steps and treatments. In the present study, an A. rhizogenes-mediated soybean root transformation approach was optimized. The method delivers significantly higher transformation efficiency levels and rates of transformed plant recovery, thus enhancing studies of soybean abiotic conditions or interactions between phytopathogens, such as nematodes. A 55 % transformation efficiency was obtained following the addition of an acclimation step that involves hydroponics and different selection processes. The present study also validated the reference genes GmELF1-b and GmELF1-a as the most stable to be used in RT-qPCR analysis i... Mostrar Tudo |
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Thesagro: |
Soja. |
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Categoria do assunto: |
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Marc: |
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100 1 $aKUMA, K. M.
245 $aA high efficient protocol for soybean root transformation by Agrobacterium rhizogenes and most stable reference genes for RT-qPCR analysis.$h[electronic resource]
260 $c2015
520 $aGene functional analyses are essential to the validation of results obtained from in silico and/or geneprospecting studies. Genetic transformation methods that yield tissues of transient expression quickly have been of considerable interest to researchers. Agrobacterium rhizogenes-mediated transformation methods, which are employed to generate plants with transformed roots, have proven useful for the study of stress caused by root phytopathogens via gene overexpression and/or silencing. While some protocols have been adapted to soybean plants, transformation efficiencies remain limited; thus, few viable plants are available for performing bioassays. Furthermore, mRNA analyses that employ reverse transcription quantitative polymerase chain reactions (RT-qPCR) require the use of reference genes with stable expression levels across different organs, development steps and treatments. In the present study, an A. rhizogenes-mediated soybean root transformation approach was optimized. The method delivers significantly higher transformation efficiency levels and rates of transformed plant recovery, thus enhancing studies of soybean abiotic conditions or interactions between phytopathogens, such as nematodes. A 55 % transformation efficiency was obtained following the addition of an acclimation step that involves hydroponics and different selection processes. The present study also validated the reference genes GmELF1-b and GmELF1-a as the most stable to be used in RT-qPCR analysis in composite plants, mainly under nematode infection.
650 $aSoja
700 1 $aLOPES-CAITAR, V. S.
700 1 $aROMERO, C. C. T.
700 1 $aSILVA, S. M. H.
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700 1 $aDIAS, W. P.
700 1 $aMARCELINO-GUIMARÃES, F. C.
773 $tPlant Cell Reports$gv. 34, n. 11, p. 1987-2000, 2015.
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