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Registro Completo |
Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Meio Ambiente. |
Data corrente: |
13/09/2022 |
Data da última atualização: |
14/09/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
LOCATELLI, G. O.; PIMENTEL, M. F.; BUENO, L. A.; LOBO JUNIOR, M.; MASCARIN, G. M.; FINKLER, C. L. L. |
Afiliação: |
GABRIEL OLIVO LOCATELLI, UNIVERSIDADE FEDERAL RURAL DE PERNAMBUCO; MARIA FERNANDA PIMENTEL, UNIVERSIDADE FEDERAL DE PERNAMBUCO; LUCIANO AVALLONE BUENO, UNIVERSIDADE FEDERAL DO ABC; MURILLO LOBO JUNIOR, CNPAF; GABRIEL MOURA MASCARIN, CNPMA; CHRISTINE LAMENHA LUNA FINKLER, UNIVERSIDADE FEDERAL DE PERNAMBUCO. |
Título: |
Production of microsclerotia by Trichoderma asperellum through submerged liquid fermentation using low-cost nitrogen and carbon sources. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Biocatalysis and Agricultural Biotechnology, v. 44, article 102455, 2022. |
ISSN: |
1878-8181 |
DOI: |
https://doi.org/10.1016/j.bcab.2022.102455 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: The aim of this work is to optimize the nutritional conditions using low-cost carbon and nitrogen sources for microsclerotia (MS) production of two Brazilian Trichoderma asperellum strains (BRM-29576 and BRM-29104). A fractional factorial design was used to investigate five variables: carbon source, carbon concentration, nitrogen source, C:N ratio and fungal strain. In this study, the optimal nutritional conditions for improving MS yield were achieved using sucrose and autolyzed yeast, carbon concentration at 20 g L-1 and C:N ratio of 10:1 for the BRM-29104 strain, which outperformed the other strain and yielded up to 2.5 (±0.9) × 104 MS mL-1 within 7 days of fermentation. Sucrose and autolyzed yeast are two economically available carbon and nitrogen sources in Brazil that are suitable for MS production by T. asperellum BRM-29104. Vacuum filtration of MS biomass through nylon filter (200 mesh/74 micrometer) increased MS concentration, resulting in up to 1.8 (±0.2) × 105 MS g-1. Our results support a cost-efficient liquid medium to achieve high MS yields by strain BRM-29104 in combination with an easy method to harvest and concentrate MS from culture broth for further formulation development. |
Palavras-Chave: |
Autolyzed yeast; Biofungicides; C N ratio; Microsclerotia. |
Thesagro: |
Carbono; Farinha; Fermentação; Fungo Para Controle Biológico; Nitrogênio; Sorgo; Trichoderma. |
Thesaurus Nal: |
Biological control agents; Liquid state fermentation; Sorghum flour; Trichoderma asperellum. |
Categoria do assunto: |
H Saúde e Patologia X Pesquisa, Tecnologia e Engenharia |
Marc: |
LEADER 02407naa a2200385 a 4500 001 2146414 005 2022-09-14 008 2022 bl uuuu u00u1 u #d 022 $a1878-8181 024 7 $ahttps://doi.org/10.1016/j.bcab.2022.102455$2DOI 100 1 $aLOCATELLI, G. O. 245 $aProduction of microsclerotia by Trichoderma asperellum through submerged liquid fermentation using low-cost nitrogen and carbon sources.$h[electronic resource] 260 $c2022 520 $aAbstract: The aim of this work is to optimize the nutritional conditions using low-cost carbon and nitrogen sources for microsclerotia (MS) production of two Brazilian Trichoderma asperellum strains (BRM-29576 and BRM-29104). A fractional factorial design was used to investigate five variables: carbon source, carbon concentration, nitrogen source, C:N ratio and fungal strain. In this study, the optimal nutritional conditions for improving MS yield were achieved using sucrose and autolyzed yeast, carbon concentration at 20 g L-1 and C:N ratio of 10:1 for the BRM-29104 strain, which outperformed the other strain and yielded up to 2.5 (±0.9) × 104 MS mL-1 within 7 days of fermentation. Sucrose and autolyzed yeast are two economically available carbon and nitrogen sources in Brazil that are suitable for MS production by T. asperellum BRM-29104. Vacuum filtration of MS biomass through nylon filter (200 mesh/74 micrometer) increased MS concentration, resulting in up to 1.8 (±0.2) × 105 MS g-1. Our results support a cost-efficient liquid medium to achieve high MS yields by strain BRM-29104 in combination with an easy method to harvest and concentrate MS from culture broth for further formulation development. 650 $aBiological control agents 650 $aLiquid state fermentation 650 $aSorghum flour 650 $aTrichoderma asperellum 650 $aCarbono 650 $aFarinha 650 $aFermentação 650 $aFungo Para Controle Biológico 650 $aNitrogênio 650 $aSorgo 650 $aTrichoderma 653 $aAutolyzed yeast 653 $aBiofungicides 653 $aC N ratio 653 $aMicrosclerotia 700 1 $aPIMENTEL, M. F. 700 1 $aBUENO, L. A. 700 1 $aLOBO JUNIOR, M. 700 1 $aMASCARIN, G. M. 700 1 $aFINKLER, C. L. L. 773 $tBiocatalysis and Agricultural Biotechnology$gv. 44, article 102455, 2022.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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Biblioteca(s): |
Embrapa Algodão. |
Data corrente: |
02/07/2014 |
Data da última atualização: |
17/07/2014 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
CARVALHO, L. P. de; MEDEIROS, E. P. de. |
Afiliação: |
LUIZ PAULO DE CARVALHO, CNPA; EVERALDO PAULO DE MEDEIROS, CNPA. |
Título: |
Avaliação de linhagens selecionadas para alto teor de óleo quanto aos caracteres de fibra. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
In: Congresso Brasileiro do Algodão, 9., 2013, Brasília, DF. |
Idioma: |
Português |
Palavras-Chave: |
LINHAGENS. |
Thesagro: |
Algodão; Fibra; Melhoramento Vegetal; Óleo. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/104398/1/melho-veg118550.pdf
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Marc: |
LEADER 00537nam a2200169 a 4500 001 1989585 005 2014-07-17 008 2013 bl uuuu u00u1 u #d 100 1 $aCARVALHO, L. P. de 245 $aAvaliação de linhagens selecionadas para alto teor de óleo quanto aos caracteres de fibra.$h[electronic resource] 260 $aIn: Congresso Brasileiro do Algodão, 9., 2013, Brasília$c2013 650 $aAlgodão 650 $aFibra 650 $aMelhoramento Vegetal 650 $aÓleo 653 $aLINHAGENS 700 1 $aMEDEIROS, E. P. de
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Embrapa Algodão (CNPA) |
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