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 | Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Algodão; Embrapa Semiárido. |
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Data corrente: |
17/10/2017 |
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Data da última atualização: |
26/12/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, J. W. M. dos; SILVA, J. F. da; FERREIRA, T. D. dos S.; DIAS, M. A. M.; FRAIZ, A. C. R.; ESCOBAR, I. E. C.; SANTOS, R. C. dos; LIMA, L. M. de; MORGANTE, C. V.; FERNANDES JUNIOR, P. I. |
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Afiliação: |
JONNATHAN WHINY MORAES DOS SANTOS, UNIVERSIDADE ESTADUAL DA PARAIBA; JÉSSICA FERNANDA DA SILVA, UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO; TAINÁ DOURADO DOS SANTOS FERREIRA, UNIVERSIDADE ESTADUAL DA BAHIA; MARCOS ANDRÉ MOURA DIAS, UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO; ANA CARLA RESENDE FRAIZ, UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO; INDIRA ELENA COSTA ESCOBAR, UNIVERSIDADE FEDERAL DO VALE DO SÃO FANCISCO; ROSEANE CAVALCANTI DOS SANTOS, CNPA; LIZIANE MARIA DE LIMA, CNPA; CAROLINA VIANNA MORGANTE, CPATSA; PAULO IVAN FERNANDES JUNIOR, CPATSA. |
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Título: |
Molecular and symbiotic characterization of peanut bradyrhizobia from the semi-arid region of Brazil. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Applied Soil Ecology, v. 121, p. 177-184, 2017. |
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Idioma: |
Inglês |
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Conteúdo: |
Peanut (Arachis hypogaea L.) is a legume crop native from South America and grown worldwide. This is well adapted to the unfavorable edaphoclimatic conditions found in Brazilian Semi-Arid region, where is cultivated mainly by smallholders. The selection of new rhizobial isolates is important to increase the rates of biological nitrogen fixation and yield. Furthermore, the diversity evaluation of new bacterial isolates may help us to understand the ecology of these groups in regions where little information are available. In this context, this study aimed to evaluate the phylogenetic relationships of peanut bradyrhizobia from Brazilian Semi-Arid soils by analysis of 16S rRNA, recA, nodC and nifD gene sequences, as well as their symbiotic performance. A trap-host experiment was performed using two soil samples from the municipality of Barbalha, Ceará, Brazil, taken under peanut cropping and one soil sample from the municipality of Juazeiro, Bahia, Brazil, covered by the native Arachis triseminata. Seeds of peanut cv. BR1 were sown and the plants were grown until 52 days after emergence. The nodules were inoculated in YMA medium for isolation of slow-growing bacteria. The Box-PCR fingerprinting and the 16S rRNA gene sequences was evaluated for all bacteria and recA, nodC and nifD were sequenced for the six selected isolates. Selected bacteria were evaluated according to their symbiotic characteristics in a greenhouse experiment. Ten slow-growing isolates were obtained and classified within the genus Bradyrhizobium, by the 16S rRNA sequence analysis and showed divergent Box-PCR profiles. All bacteria were clustered within the B. japonicum clade. The bacteria from Juazeiro were closely related to B. yuanmingense, and those from Barbalha showed similarity to B. yuanmingense, B. kavangense and B. guangxiense. The sequences of recA showed partial congruence to the 16S rRNA gene analysis but the phylogeny trees based on the symbiotic genes were not related, indicating horizontal gene transfer. All bacteria were able to efficiently nodulate the peanut and the isolate ESA 83, pointed out regarding the nitrogen fixation ability and were selected for further analysis in nonsterile soils and field conditions. MenosPeanut (Arachis hypogaea L.) is a legume crop native from South America and grown worldwide. This is well adapted to the unfavorable edaphoclimatic conditions found in Brazilian Semi-Arid region, where is cultivated mainly by smallholders. The selection of new rhizobial isolates is important to increase the rates of biological nitrogen fixation and yield. Furthermore, the diversity evaluation of new bacterial isolates may help us to understand the ecology of these groups in regions where little information are available. In this context, this study aimed to evaluate the phylogenetic relationships of peanut bradyrhizobia from Brazilian Semi-Arid soils by analysis of 16S rRNA, recA, nodC and nifD gene sequences, as well as their symbiotic performance. A trap-host experiment was performed using two soil samples from the municipality of Barbalha, Ceará, Brazil, taken under peanut cropping and one soil sample from the municipality of Juazeiro, Bahia, Brazil, covered by the native Arachis triseminata. Seeds of peanut cv. BR1 were sown and the plants were grown until 52 days after emergence. The nodules were inoculated in YMA medium for isolation of slow-growing bacteria. The Box-PCR fingerprinting and the 16S rRNA gene sequences was evaluated for all bacteria and recA, nodC and nifD were sequenced for the six selected isolates. Selected bacteria were evaluated according to their symbiotic characteristics in a greenhouse experiment. Ten slow-growing isolates were obtained and class... Mostrar Tudo |
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Palavras-Chave: |
Arachis triseminata; Biological nitrogen fixation; Bradyrhizobia; FBN; Fixação biológica do nitrogênio; Inoculant; Peanut; Rizóbio. |
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Thesagro: |
Amendoim; Arachis Hypogaea; Bactéria; Fixação simbiótica de nitrogênio; Leguminosa; Solo. |
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Thesaurus Nal: |
Bradyrhizobium; Nitrogen fixation; Peanuts. |
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Categoria do assunto: |
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X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 03473naa a2200433 a 4500
001 2082806
005 2017-12-26
008 2017 bl uuuu u00u1 u #d
100 1 $aSANTOS, J. W. M. dos
245 $aMolecular and symbiotic characterization of peanut bradyrhizobia from the semi-arid region of Brazil.$h[electronic resource]
260 $c2017
520 $aPeanut (Arachis hypogaea L.) is a legume crop native from South America and grown worldwide. This is well adapted to the unfavorable edaphoclimatic conditions found in Brazilian Semi-Arid region, where is cultivated mainly by smallholders. The selection of new rhizobial isolates is important to increase the rates of biological nitrogen fixation and yield. Furthermore, the diversity evaluation of new bacterial isolates may help us to understand the ecology of these groups in regions where little information are available. In this context, this study aimed to evaluate the phylogenetic relationships of peanut bradyrhizobia from Brazilian Semi-Arid soils by analysis of 16S rRNA, recA, nodC and nifD gene sequences, as well as their symbiotic performance. A trap-host experiment was performed using two soil samples from the municipality of Barbalha, Ceará, Brazil, taken under peanut cropping and one soil sample from the municipality of Juazeiro, Bahia, Brazil, covered by the native Arachis triseminata. Seeds of peanut cv. BR1 were sown and the plants were grown until 52 days after emergence. The nodules were inoculated in YMA medium for isolation of slow-growing bacteria. The Box-PCR fingerprinting and the 16S rRNA gene sequences was evaluated for all bacteria and recA, nodC and nifD were sequenced for the six selected isolates. Selected bacteria were evaluated according to their symbiotic characteristics in a greenhouse experiment. Ten slow-growing isolates were obtained and classified within the genus Bradyrhizobium, by the 16S rRNA sequence analysis and showed divergent Box-PCR profiles. All bacteria were clustered within the B. japonicum clade. The bacteria from Juazeiro were closely related to B. yuanmingense, and those from Barbalha showed similarity to B. yuanmingense, B. kavangense and B. guangxiense. The sequences of recA showed partial congruence to the 16S rRNA gene analysis but the phylogeny trees based on the symbiotic genes were not related, indicating horizontal gene transfer. All bacteria were able to efficiently nodulate the peanut and the isolate ESA 83, pointed out regarding the nitrogen fixation ability and were selected for further analysis in nonsterile soils and field conditions.
650 $aBradyrhizobium
650 $aNitrogen fixation
650 $aPeanuts
650 $aAmendoim
650 $aArachis Hypogaea
650 $aBactéria
650 $aFixação simbiótica de nitrogênio
650 $aLeguminosa
650 $aSolo
653 $aArachis triseminata
653 $aBiological nitrogen fixation
653 $aBradyrhizobia
653 $aFBN
653 $aFixação biológica do nitrogênio
653 $aInoculant
653 $aPeanut
653 $aRizóbio
700 1 $aSILVA, J. F. da
700 1 $aFERREIRA, T. D. dos S.
700 1 $aDIAS, M. A. M.
700 1 $aFRAIZ, A. C. R.
700 1 $aESCOBAR, I. E. C.
700 1 $aSANTOS, R. C. dos
700 1 $aLIMA, L. M. de
700 1 $aMORGANTE, C. V.
700 1 $aFERNANDES JUNIOR, P. I.
773 $tApplied Soil Ecology$gv. 121, p. 177-184, 2017.
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