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Registro Completo |
Biblioteca(s): |
Embrapa Agroenergia. |
Data corrente: |
23/11/2022 |
Data da última atualização: |
24/11/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ROMERO PELÁEZ, R. D.; WISCHRAL, D.; CUNHA, J. R. B.; MENDES, T. D.; PACHECO, T. F.; SIQUEIRA, F. G. de; ALMEIDA, J. R. M. de. |
Afiliação: |
RUBÉN DARÍO ROMERO PELÁEZ, UNIVERSIDADE DE BRASÍLIA; DAIANA WISCHRAL, CNPAE; JOICE RAÍSA BARBOSA CUNHA, CNPAE; THAIS DEMARCHI MENDES, CNPAE; THALYTA FRAGA PACHECO, CNPAE; FELIX GONCALVES DE SIQUEIRA, CNPAE; JOAO RICARDO MOREIRA DE ALMEIDA, CNPAE. |
Título: |
Production of enzymatic extract with high cellulolytic and oxidative activities by co-culture of Trichoderma reesei and Panus lecomtei. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Fermentation, v. 8, n. 10, 522, 2022. |
Páginas: |
17 p. |
ISSN: |
2311-5637 |
DOI: |
https://doi.org/10.3390/fermentation8100522 |
Idioma: |
Inglês |
Conteúdo: |
This work aimed to produce enzymatic fungi extracts with hydrolytic and oxidative activities to hydrolyze lignocellulosic biomasses efficiently. For this, the fungi Trichoderma reesei and Panus lecomtei were co-cultured using the vegetable biomasses oil palm decanter cake, wheat bran, and cottonseed cake as substrates in submerged fermentation. T. reesei and P. lecomtei showed partially compatible positive interaction on plates. The co-cultures respond positively to variations of temperature and inoculum interval, generating extracts responsible for higher hydrolysis yield when grown at 25 °C, and P. lecomtei is inoculated 24 h after T. reesei. The enzymatic extract production of co-cultures was also improved by modifying the components of the initial media and evaluating enzymatic activities, hydrolysis of sugarcane bagasse pretreated by autohydrolysis and ethanol production as a response. Five culture media were evaluated with variations in the composition of nutritional elements, minerals and substrates. The best extract showed a maximum cellulose hydrolysis efficiency of 68.7% compared with 44.8% of the initial medium. The ethanolic fermentation of hydrolysates obtained by co-culture extracts showed higher ethanol yields than monocultures. This work demonstrates the use of fungi co-cultures to produce enzymatic extracts composed of cellulolytic, hemicellulolytic, and ligninolytic enzymes complexes, which allow hydrolyzing pretreated lignocellulosic biomass with high efficiency, generating hydrolysates that are easier fermented by yeast. MenosThis work aimed to produce enzymatic fungi extracts with hydrolytic and oxidative activities to hydrolyze lignocellulosic biomasses efficiently. For this, the fungi Trichoderma reesei and Panus lecomtei were co-cultured using the vegetable biomasses oil palm decanter cake, wheat bran, and cottonseed cake as substrates in submerged fermentation. T. reesei and P. lecomtei showed partially compatible positive interaction on plates. The co-cultures respond positively to variations of temperature and inoculum interval, generating extracts responsible for higher hydrolysis yield when grown at 25 °C, and P. lecomtei is inoculated 24 h after T. reesei. The enzymatic extract production of co-cultures was also improved by modifying the components of the initial media and evaluating enzymatic activities, hydrolysis of sugarcane bagasse pretreated by autohydrolysis and ethanol production as a response. Five culture media were evaluated with variations in the composition of nutritional elements, minerals and substrates. The best extract showed a maximum cellulose hydrolysis efficiency of 68.7% compared with 44.8% of the initial medium. The ethanolic fermentation of hydrolysates obtained by co-culture extracts showed higher ethanol yields than monocultures. This work demonstrates the use of fungi co-cultures to produce enzymatic extracts composed of cellulolytic, hemicellulolytic, and ligninolytic enzymes complexes, which allow hydrolyzing pretreated lignocellulosic biomass with high effi... Mostrar Tudo |
Palavras-Chave: |
Co-cultures; Ethanolic fermentation; Laccases; Panus lecomtei. |
Thesaurus Nal: |
Cellulases; Trichoderma reesei. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1148635/1/Production-of-enzymatic-extract.pdf
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Marc: |
LEADER 02486naa a2200301 a 4500 001 2148635 005 2022-11-24 008 2022 bl uuuu u00u1 u #d 022 $a2311-5637 024 7 $ahttps://doi.org/10.3390/fermentation8100522$2DOI 100 1 $aROMERO PELÁEZ, R. D. 245 $aProduction of enzymatic extract with high cellulolytic and oxidative activities by co-culture of Trichoderma reesei and Panus lecomtei.$h[electronic resource] 260 $c2022 300 $a17 p. 520 $aThis work aimed to produce enzymatic fungi extracts with hydrolytic and oxidative activities to hydrolyze lignocellulosic biomasses efficiently. For this, the fungi Trichoderma reesei and Panus lecomtei were co-cultured using the vegetable biomasses oil palm decanter cake, wheat bran, and cottonseed cake as substrates in submerged fermentation. T. reesei and P. lecomtei showed partially compatible positive interaction on plates. The co-cultures respond positively to variations of temperature and inoculum interval, generating extracts responsible for higher hydrolysis yield when grown at 25 °C, and P. lecomtei is inoculated 24 h after T. reesei. The enzymatic extract production of co-cultures was also improved by modifying the components of the initial media and evaluating enzymatic activities, hydrolysis of sugarcane bagasse pretreated by autohydrolysis and ethanol production as a response. Five culture media were evaluated with variations in the composition of nutritional elements, minerals and substrates. The best extract showed a maximum cellulose hydrolysis efficiency of 68.7% compared with 44.8% of the initial medium. The ethanolic fermentation of hydrolysates obtained by co-culture extracts showed higher ethanol yields than monocultures. This work demonstrates the use of fungi co-cultures to produce enzymatic extracts composed of cellulolytic, hemicellulolytic, and ligninolytic enzymes complexes, which allow hydrolyzing pretreated lignocellulosic biomass with high efficiency, generating hydrolysates that are easier fermented by yeast. 650 $aCellulases 650 $aTrichoderma reesei 653 $aCo-cultures 653 $aEthanolic fermentation 653 $aLaccases 653 $aPanus lecomtei 700 1 $aWISCHRAL, D. 700 1 $aCUNHA, J. R. B. 700 1 $aMENDES, T. D. 700 1 $aPACHECO, T. F. 700 1 $aSIQUEIRA, F. G. de 700 1 $aALMEIDA, J. R. M. de 773 $tFermentation$gv. 8, n. 10, 522, 2022.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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Biblioteca(s): |
Embrapa Clima Temperado; Embrapa Hortaliças. |
Data corrente: |
19/02/2015 |
Data da última atualização: |
19/02/2015 |
Tipo da produção científica: |
Boletim de Pesquisa e Desenvolvimento |
Autoria: |
SILVA, G. O. da; PEREIRA, A. da S.; SUINAGA, F. A.; CARVALHO, A. D. F. de. |
Afiliação: |
GIOVANI OLEGARIO DA SILVA, CNPH; ARIONE DA SILVA PEREIRA, CPACT; FABIO AKIYOSHI SUINAGA, CNPH; AGNALDO D FERREIRA DE CARVALHO, CNPH. |
Título: |
Importância de caracteres na dissimilaridade de progênies de batata em primeira geração clonal. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
Brasília, DF: Embrapa Hortaliças, 2014. |
Páginas: |
16 p. |
Série: |
(Embrapa Hortaliças. Boletim de Pesquisa e Desenvolvimento, 114). |
ISSN: |
1677-2229 |
Idioma: |
Português |
Conteúdo: |
O trabalho teve por objetivo verificar as implicações da utilização da análise de importância de caracteres na eliminação de caracteres avaliados, para o cálculo da distância genética de progênies de batata (Solanum tuberosum L.) na primeira geração clonal. |
Palavras-Chave: |
Contribuição relativa de caracteres; Solanum tuberosum L. |
Thesagro: |
Batata. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/118234/1/BPD-114.pdf
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Marc: |
LEADER 00985nam a2200217 a 4500 001 2009108 005 2015-02-19 008 2014 bl uuuu u0uu1 u #d 022 $a1677-2229 100 1 $aSILVA, G. O. da 245 $aImportância de caracteres na dissimilaridade de progênies de batata em primeira geração clonal.$h[electronic resource] 260 $aBrasília, DF: Embrapa Hortaliças$c2014 300 $a16 p. 490 $a(Embrapa Hortaliças. Boletim de Pesquisa e Desenvolvimento, 114). 520 $aO trabalho teve por objetivo verificar as implicações da utilização da análise de importância de caracteres na eliminação de caracteres avaliados, para o cálculo da distância genética de progênies de batata (Solanum tuberosum L.) na primeira geração clonal. 650 $aBatata 653 $aContribuição relativa de caracteres 653 $aSolanum tuberosum L 700 1 $aPEREIRA, A. da S. 700 1 $aSUINAGA, F. A. 700 1 $aCARVALHO, A. D. F. de
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