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Registro Completo |
Biblioteca(s): |
Embrapa Café. |
Data corrente: |
08/01/2024 |
Data da última atualização: |
08/01/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
PRADO, G. S.; ROCHA, D. C.; SANTOS, L. N. dos; CONTILIANI, D. F.; NOBILE, P. M.; MARTINATI-SCHENK, J. C.; PADILHA, L.; MALUF, M. P.; LUBINI, G.; PEREIRA, T. C.; MONTEIRO-VITORELLO, C. B.; CRESTE, S.; BOSCARIOL-CAMARGO, R. L.; TAKITA, M. A.; CRISTOFANI-YALY, M.; SOUZA, A. A. de. |
Afiliação: |
GUILHERME SOUZA PRADO, INSTITUTO AGRONÔMICO; DHIÔVANNA CORRÊIA ROCHA, UNIVERSIDADE ESTADUAL DE CAMPINAS; LUCAS NASCIMENTO DOS SANTOS, INSTITUTO AGRONÔMICO; DANYEL FERNANDES CONTILIANI, INSTITUTO AGRONÔMICO; PAULA MACEDO NOBILE, INSTITUTO AGRONÔMICO; JULIANA CAMARGO MARTINATI-SCHENK, INSTITUTO AGRONÔMICO; LILIAN PADILHA, CNPCa; MIRIAN PEREZ MALUF, CNPCa; GREICE LUBINI, INSTITUTO AGRONÔMICO; TIAGO CAMPOS PEREIRA, UNIVERSIDADE DE SÃO PAULO; CLAUDIA BARROS MONTEIRO-VITORELLO, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; SILVANA CRESTE, INSTITUTO AGRONÔMICO; RAQUEL LUCIANA BOSCARIOL-CAMARGO, INSTITUTO AGRONÔMICO; MARCO AURÉLIO TAKITA, INSTITUTO AGRONÔMICO; MARIÂNGELA CRISTOFANI-YALY, INSTITUTO AGRONÔMICO; ALESSANDRA ALVES DE SOUZA, INSTITUTO AGRONÔMICO. |
Título: |
CRISPR technology towards genome editing of the perennial and semi-perennial crops citrus, coffee and sugarcane. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Frontiers in Plant Science, v. 14, article 1331258, 2023. |
DOI: |
https://doi.org/10.3389/fpls.2023.1331258 |
Idioma: |
Inglês |
Conteúdo: |
Gene editing technologies have opened up the possibility of manipulating the genome of any organism in a predicted way. CRISPR technology is the most used genome editing tool and, in agriculture, it has allowed the expansion of possibilities in plant biotechnology, such as gene knockout or knock-in, transcriptional regulation, epigenetic modification, base editing, RNA editing, prime editing, and nucleic acid probing or detection. This technology mostly depends on in vitro tissue culture and genetic transformation/transfection protocols, which sometimes become the major challenges for its application in different crops. Agrobacterium-mediated transformation, biolistics, plasmid or RNP (ribonucleoprotein) transfection of protoplasts are some of the commonly used CRISPR delivery methods, but they depend on the genotype and target gene for efficient editing. The choice of the CRISPR system (Cas9, Cas12), CRISPR mechanism (plasmid or RNP) and transfection technique (Agrobacterium spp., PEG solution, lipofection) directly impacts the transformation efficiency and/or editing rate. Besides, CRISPR/Cas technology has made countries rethink regulatory frameworks concerning genetically modified organisms and flexibilize regulatory obstacles for edited plants. Here we present an overview of the state-of-the-art of CRISPR technology applied to three important crops worldwide (citrus, coffee and sugarcane), considering the biological, methodological, and regulatory aspects of its application. In addition, we provide perspectives on recently developed CRISPR tools and promising applications for each of these crops, thus highlighting the usefulness of gene editing to develop novel cultivars. MenosGene editing technologies have opened up the possibility of manipulating the genome of any organism in a predicted way. CRISPR technology is the most used genome editing tool and, in agriculture, it has allowed the expansion of possibilities in plant biotechnology, such as gene knockout or knock-in, transcriptional regulation, epigenetic modification, base editing, RNA editing, prime editing, and nucleic acid probing or detection. This technology mostly depends on in vitro tissue culture and genetic transformation/transfection protocols, which sometimes become the major challenges for its application in different crops. Agrobacterium-mediated transformation, biolistics, plasmid or RNP (ribonucleoprotein) transfection of protoplasts are some of the commonly used CRISPR delivery methods, but they depend on the genotype and target gene for efficient editing. The choice of the CRISPR system (Cas9, Cas12), CRISPR mechanism (plasmid or RNP) and transfection technique (Agrobacterium spp., PEG solution, lipofection) directly impacts the transformation efficiency and/or editing rate. Besides, CRISPR/Cas technology has made countries rethink regulatory frameworks concerning genetically modified organisms and flexibilize regulatory obstacles for edited plants. Here we present an overview of the state-of-the-art of CRISPR technology applied to three important crops worldwide (citrus, coffee and sugarcane), considering the biological, methodological, and regulatory aspects of its applica... Mostrar Tudo |
Thesaurus Nal: |
Citrus; Coffee beans; Genome; Perennials; Sugarcane. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1160555/1/CRISPR-technology-towards.pdf
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Marc: |
LEADER 02801naa a2200373 a 4500 001 2160555 005 2024-01-08 008 2023 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.3389/fpls.2023.1331258$2DOI 100 1 $aPRADO, G. S. 245 $aCRISPR technology towards genome editing of the perennial and semi-perennial crops citrus, coffee and sugarcane.$h[electronic resource] 260 $c2023 520 $aGene editing technologies have opened up the possibility of manipulating the genome of any organism in a predicted way. CRISPR technology is the most used genome editing tool and, in agriculture, it has allowed the expansion of possibilities in plant biotechnology, such as gene knockout or knock-in, transcriptional regulation, epigenetic modification, base editing, RNA editing, prime editing, and nucleic acid probing or detection. This technology mostly depends on in vitro tissue culture and genetic transformation/transfection protocols, which sometimes become the major challenges for its application in different crops. Agrobacterium-mediated transformation, biolistics, plasmid or RNP (ribonucleoprotein) transfection of protoplasts are some of the commonly used CRISPR delivery methods, but they depend on the genotype and target gene for efficient editing. The choice of the CRISPR system (Cas9, Cas12), CRISPR mechanism (plasmid or RNP) and transfection technique (Agrobacterium spp., PEG solution, lipofection) directly impacts the transformation efficiency and/or editing rate. Besides, CRISPR/Cas technology has made countries rethink regulatory frameworks concerning genetically modified organisms and flexibilize regulatory obstacles for edited plants. Here we present an overview of the state-of-the-art of CRISPR technology applied to three important crops worldwide (citrus, coffee and sugarcane), considering the biological, methodological, and regulatory aspects of its application. In addition, we provide perspectives on recently developed CRISPR tools and promising applications for each of these crops, thus highlighting the usefulness of gene editing to develop novel cultivars. 650 $aCitrus 650 $aCoffee beans 650 $aGenome 650 $aPerennials 650 $aSugarcane 700 1 $aROCHA, D. C. 700 1 $aSANTOS, L. N. dos 700 1 $aCONTILIANI, D. F. 700 1 $aNOBILE, P. M. 700 1 $aMARTINATI-SCHENK, J. C. 700 1 $aPADILHA, L. 700 1 $aMALUF, M. P. 700 1 $aLUBINI, G. 700 1 $aPEREIRA, T. C. 700 1 $aMONTEIRO-VITORELLO, C. B. 700 1 $aCRESTE, S. 700 1 $aBOSCARIOL-CAMARGO, R. L. 700 1 $aTAKITA, M. A. 700 1 $aCRISTOFANI-YALY, M. 700 1 $aSOUZA, A. A. de 773 $tFrontiers in Plant Science$gv. 14, article 1331258, 2023.
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Embrapa Café (CNPCa) |
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Biblioteca(s): |
Embrapa Trigo. |
Data corrente: |
17/07/2018 |
Data da última atualização: |
05/10/2018 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
CARRÃO-PANIZZI, M. C. |
Afiliação: |
MERCEDES CONCORDIA CARRAO PANIZZI, CNPT. |
Título: |
Soja hortaliça uma opção além da "commodity". |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
In: SIMPÓSIO DE SEGURANÇA ALIMENTAR, 6., 2018, Gramado. Desvendando mitos... Porto Alegre: UFRGS; Campinas: SBCTA, 2018. |
Páginas: |
6 p. |
Idioma: |
Português |
Notas: |
Produção Sustentável de Alimentos, trab. 76. |
Palavras-Chave: |
Edamame; Isoflavona; Soja verde. |
Thesagro: |
Broto de Soja; Sabor; Soja. |
Thesaurus NAL: |
Flavor; Isoflavones; Soybeans; Sprouts (food). |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/179895/1/ID44352-2018SSA6AC76.pdf
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Marc: |
LEADER 00731nam a2200241 a 4500 001 2093353 005 2018-10-05 008 2018 bl uuuu u00u1 u #d 100 1 $aCARRÃO-PANIZZI, M. C. 245 $aSoja hortaliça uma opção além da "commodity".$h[electronic resource] 260 $aIn: SIMPÓSIO DE SEGURANÇA ALIMENTAR, 6., 2018, Gramado. Desvendando mitos... Porto Alegre: UFRGS; Campinas: SBCTA$c2018 300 $a6 p. 500 $aProdução Sustentável de Alimentos, trab. 76. 650 $aFlavor 650 $aIsoflavones 650 $aSoybeans 650 $aSprouts (food) 650 $aBroto de Soja 650 $aSabor 650 $aSoja 653 $aEdamame 653 $aIsoflavona 653 $aSoja verde
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