Data/hora: 02/06/2024 07:08:34

Biblioteca(s): 	Embrapa Trigo.	
Tipo da produção científica: 	Artigo em Periódico Indexado	
Circulação/Nível: 	A - 1	
Data corrente: 	06/12/2021	
Data da última atualização: 	06/12/2021	
Autoria: 	DIMKIĆ, I.; BHARDWAJ, V.; CARPENTIERI-PIPOLO, V.; KUZMANOVIĆ, N.; DEGRASSI, G.	
Afiliação: 	IVICA DIMKIĆ, Department of Biochemistry and Molecular Biology, University of Belgrade – Faculty of Biology, Belgrade, Serbia; VIBHA BHARDWAJ, Ras Al Khaimah Municipality Department, Director Environment Laboratories, Dubai, United Arab Emirate; VALERIA CARPENTIERI PIPOLO, CNPT; NEMANJA KUZMANOVIĆ, Federal Research Centre for Cultivated Plants (JKI), Institute for Plant Protection in Horticulture and Forests, Julius Ku¨ hn-Institut, Braunschweig, Germany; GIULIANO DEGRASSI, Industrial Biotechnology Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), Buenos Aires, Argentina.	
Título: 	The chitinolytic activity of the Curtobacterium sp. isolated from field-grown soybean and analysis of its genome sequence.	
Ano de publicação: 	2021	
Fonte/Imprenta: 	Plos One, v. 16, n.11, e0259465 Jan. 2021.	
DOI: 	https://doi.org/10.1371/journal. pone.0259465	
Idioma: 	Inglês	
Conteúdo: 	Curtobacterium sp. GD1 was isolated from leaves of conventionally grown soybean in Brazil. It was noteworthy that among all bacteria previously isolated from the same origin, only Curtobacterium sp. GD1 showed a strong chitinase activity. The enzyme was secreted and its production was induced by the presence of colloidal chitin in the medium. The chitinase was partially purified and characterized: molecular weight was approximately 37 kDa and specific activity 90.8 U/mg. Furthermore, Curtobacterium sp. GD1 genome was sequenced and analyzed. Our isolate formed a phylogenetic cluster with four other Curtobacterium spp. strains, with ANIb/ANIm &#65533; 98%, representing a new, still non described Curtobacterium species. The circular genome visualization and comparison of genome sequences of strains forming new cluster indicated that most regions within their genomes were highly conserved. The gene associated with chitinase production was identified and the distribution pattern of glycosyl hydrolases genes was assessed. Also, genes associated with catabolism of structural carbohydrates such as oligosaccharides, mixed polysaccharides, plant and animal polysaccharides, as well as genes or gene clusters associated with resistance to antibiotics, toxic compounds and auxin biosynthesis subsystem products were identified. The abundance of putative glycosyl hydrolases in the genome of Curtobacterium sp. GD1 suggests that it has the tools for the hydrolysis of different polysaccharides. Therefore, Curtobacterium sp. GD1 isolated from soybean might be a bioremediator, biocontrol agent, an elicitor of the plant defense responses or simply degrader.	
Palavras-Chave: 	Curtobacterium sp; Enzyme; GD1.	
Thesaurus Nal: 	Soybeans.	
Categoria do assunto: 	F Plantas e Produtos de Origem Vegetal	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/228529/1/journal.pone.0259465-PLOSONE-2021.pdf	
Registro original: 	Embrapa Trigo (CNPT)	
Exemplares: 	CNPT - (45251-1) - UPC - AP - DD -  -  -  - DIS