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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
12/08/2005 |
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Data da última atualização: |
22/12/2021 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
ALVES, G. M. R.; COSTA, J. L. da S. |
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Afiliação: |
JEFFERSON LUIS DA SILVA COSTA, CNPAF. |
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Título: |
Fusarium sp. como possível agente de controle biológico de Bidens pilosa. |
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Ano de publicação: |
2001 |
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Fonte/Imprenta: |
Fitopatologia Brasileira, v. 26, p. 366, ago. 2001. Suplemento, ref. 369. Edição dos Resumos do XXXIV Congresso Brasileiro de Fitopatologia, São Pedro, ago. 2001. |
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Idioma: |
Português |
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Conteúdo: |
O picão preto e uma das mais serias infestantes de culturas em mais de 40 países. Recentemente encontrou-se no Brasil, áreas com Bidens pilosa atacadas por uma fusariose. Para avaliar o efeito do Fusarium sp. no controle da planta daninha B. pilosa, 100 plantas foram pulverizadas com filtrado do crescimento micelial, para controle 100 plantas foram pulverizadas com agua. |
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Palavras-Chave: |
Fusarium sp. |
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Thesagro: |
Bidens Pilosa; Controle Biológico; Picão Preto. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/229569/1/2001-cbf.pdf
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Marc: |
LEADER 00985nam a2200169 a 4500
001 1208066
005 2021-12-22
008 2001 bl uuuu u00u1 u #d
100 1 $aALVES, G. M. R.
245 $aFusarium sp. como possível agente de controle biológico de Bidens pilosa.
260 $aFitopatologia Brasileira, v. 26, p. 366, ago. 2001. Suplemento, ref. 369. Edição dos Resumos do XXXIV Congresso Brasileiro de Fitopatologia, São Pedro, ago. 2001.$c2001
520 $aO picão preto e uma das mais serias infestantes de culturas em mais de 40 países. Recentemente encontrou-se no Brasil, áreas com Bidens pilosa atacadas por uma fusariose. Para avaliar o efeito do Fusarium sp. no controle da planta daninha B. pilosa, 100 plantas foram pulverizadas com filtrado do crescimento micelial, para controle 100 plantas foram pulverizadas com agua.
650 $aBidens Pilosa
650 $aControle Biológico
650 $aPicão Preto
653 $aFusarium sp
700 1 $aCOSTA, J. L. da S.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
07/08/2020 |
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Data da última atualização: |
27/10/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
PINHEIRO, D. H.; MOREIRA, R. O.; LEITE, N. A.; REDOAN, A. C.; XAVIER, A. da S.; BARROS, B. de A.; CARNEIRO, N. P. |
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Afiliação: |
Daniele Heloisa Pinheiro; Raquel Oliveira Moreira, UNESP; Natalia Alves Leite, Universidade Federal do Rio Grande do Sul; Ana Carolina Redoan; Andre da Silva Xavier, Universidade Federal do Espírito Santo; BEATRIZ DE ALMEIDA BARROS, CNPMS; NEWTON PORTILHO CARNEIRO, CNPMS. |
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Título: |
Suitable reference genes for RT-qPCR analysis in Dichelops melacanthus (Hemiptera: Pentatomidae). |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Molecular Biology Reports, v. 47, p. 4989-5000, 2020. |
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DOI: |
10.1007/s11033-020-05550-z |
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Idioma: |
Inglês |
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Conteúdo: |
The relative quantification of gene expression is mainly realized through reverse transcription-quantitative PCR (RT-qPCR). However, the accuracy of this technique is deeply influenced by the expression stability of the reference genes used for data normalization. Therefore, the selection of suitable reference genes for a given experimental condition is a prerequisite in gene expression studies.Dichelops melacanthus(Hemiptera: Pentatomidae) is an important phloem sap-sucking insect pest of soybean, wheat, and maize in Brazil. Most of the genetic and molecular biology studies require gene expression analysis. Nevertheless, there are no reports about reference genes for RT-qPCR data normalization inD. melacanthus. In this study, we evaluated the expression stability of nine candidate reference genes (nadh,sdhb,gapdh,fau,ef1a,rpl9,ube4a,gusandrps23) in different developmental stages, body parts, sex, starvation-induced stress and dsRNA exposure by RefFinder software that integrates the statistical algorithms geNorm, NormFinder, BestKeeper, and Delta Ct method. Our results showed that ef1a and nadh are the most stable reference genes for developmental stages,fauandrps23for sex,ube4aandrps23for body parts,rpl9andfaufor starvation stress, andnadhandsdhbfor dsRNA exposure treatment. The reference genes selected in this work will be useful for further RT-qPCR analyses onD. melacanthus, facilitating future gene expression studies that can provide a better understanding of the developmental, physiological, and molecular processes of this important insect pest. Moreover, the knowledge gained from these studies can be helpful to design effective and sustainable pest management strategies. MenosThe relative quantification of gene expression is mainly realized through reverse transcription-quantitative PCR (RT-qPCR). However, the accuracy of this technique is deeply influenced by the expression stability of the reference genes used for data normalization. Therefore, the selection of suitable reference genes for a given experimental condition is a prerequisite in gene expression studies.Dichelops melacanthus(Hemiptera: Pentatomidae) is an important phloem sap-sucking insect pest of soybean, wheat, and maize in Brazil. Most of the genetic and molecular biology studies require gene expression analysis. Nevertheless, there are no reports about reference genes for RT-qPCR data normalization inD. melacanthus. In this study, we evaluated the expression stability of nine candidate reference genes (nadh,sdhb,gapdh,fau,ef1a,rpl9,ube4a,gusandrps23) in different developmental stages, body parts, sex, starvation-induced stress and dsRNA exposure by RefFinder software that integrates the statistical algorithms geNorm, NormFinder, BestKeeper, and Delta Ct method. Our results showed that ef1a and nadh are the most stable reference genes for developmental stages,fauandrps23for sex,ube4aandrps23for body parts,rpl9andfaufor starvation stress, andnadhandsdhbfor dsRNA exposure treatment. The reference genes selected in this work will be useful for further RT-qPCR analyses onD. melacanthus, facilitating future gene expression studies that can provide a better understanding of the develop... Mostrar Tudo |
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Thesagro: |
Gene; Inseto; Percevejo; Seleção Genética. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02446naa a2200253 a 4500
001 2124236
005 2020-10-27
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1007/s11033-020-05550-z$2DOI
100 1 $aPINHEIRO, D. H.
245 $aSuitable reference genes for RT-qPCR analysis in Dichelops melacanthus (Hemiptera$bPentatomidae).$h[electronic resource]
260 $c2020
520 $aThe relative quantification of gene expression is mainly realized through reverse transcription-quantitative PCR (RT-qPCR). However, the accuracy of this technique is deeply influenced by the expression stability of the reference genes used for data normalization. Therefore, the selection of suitable reference genes for a given experimental condition is a prerequisite in gene expression studies.Dichelops melacanthus(Hemiptera: Pentatomidae) is an important phloem sap-sucking insect pest of soybean, wheat, and maize in Brazil. Most of the genetic and molecular biology studies require gene expression analysis. Nevertheless, there are no reports about reference genes for RT-qPCR data normalization inD. melacanthus. In this study, we evaluated the expression stability of nine candidate reference genes (nadh,sdhb,gapdh,fau,ef1a,rpl9,ube4a,gusandrps23) in different developmental stages, body parts, sex, starvation-induced stress and dsRNA exposure by RefFinder software that integrates the statistical algorithms geNorm, NormFinder, BestKeeper, and Delta Ct method. Our results showed that ef1a and nadh are the most stable reference genes for developmental stages,fauandrps23for sex,ube4aandrps23for body parts,rpl9andfaufor starvation stress, andnadhandsdhbfor dsRNA exposure treatment. The reference genes selected in this work will be useful for further RT-qPCR analyses onD. melacanthus, facilitating future gene expression studies that can provide a better understanding of the developmental, physiological, and molecular processes of this important insect pest. Moreover, the knowledge gained from these studies can be helpful to design effective and sustainable pest management strategies.
650 $aGene
650 $aInseto
650 $aPercevejo
650 $aSeleção Genética
700 1 $aMOREIRA, R. O.
700 1 $aLEITE, N. A.
700 1 $aREDOAN, A. C.
700 1 $aXAVIER, A. da S.
700 1 $aBARROS, B. de A.
700 1 $aCARNEIRO, N. P.
773 $tMolecular Biology Reports$gv. 47, p. 4989-5000, 2020.
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