Data/hora: 24/06/2024 19:02:10

Biblioteca(s): 	Embrapa Milho e Sorgo.	
Data corrente: 	27/10/2008	
Data da última atualização: 	24/05/2018	
Autoria: 	COELHO, M. R. R.; VOS, M. de; CARNEIRO, N. P.; MARRIEL, I. E.; PAIVA, E.; SELDIN, L.	
Afiliação: 	Marcia Reed Rodrigues Coelho, UFRJ; Marjon de Vos, UFRJ/Wagening Universiy; NEWTON PORTILHO CARNEIRO, CNPMS; IVANILDO EVODIO MARRIEL, CNPMS; Edilson Paiva Embrapa, CNPMS; Lucy Seldin, UFRJ.	
Título: 	Diversity of nifH gene pools in the rhizosphere of two cultivars of sorghum (Sorghum bicolor) treated with contrasting levels of nitrogen fertilizer.	
Ano de publicação: 	2008	
Fonte/Imprenta: 	FEMS Microbiology Letters, Amsterdam, v. 279, n. 1, p. 15-22, 2008.	
DOI: 	10.1111/j.1574-6968.2007.00975.x	
Idioma: 	Inglês	
Conteúdo: 	The diversity of nitrogen-fixing bacteria was assessed in the rhizospheres of two cultivars of sorghum (IS 5322-C and IPA 1011) sown in Cerrado soil amended with two levels of nitrogen fertilizer (12 and 120 kg ha-1). The nifH gene was amplified directly from DNA extracted from the rhizospheres, and the PCR products cloned and sequenced. Four clone libraries were generated from the nifH fragments and 245 sequences were obtained. Most of the clones (57%) were closely related to nifH genes of uncultured bacteria. NifH clones affiliated with Azohydromonas spp., Ideonella sp., Rhizobium etli and Bradyrhizobium sp. were found in all libraries. Sequences affiliated with Delftia tsuruhatensis were found in the rhizosphere of both cultivars sown with high levels of nitrogen, while clones affiliated with Methylocystis sp. were detected only in plants sown under low levels of nitrogen. Moreover, clones affiliated with Paenibacillus durus could be found in libraries from the cultivar IS 5322-C sown either in high or low amounts of fertilizer. This study showed that the amount of nitrogen used for fertilization is the overriding determinative factor that influenced the nitrogen-fixing community structures in sorghum rhizospheres cultivated in Cerrado soil.	
Palavras-Chave: 	Diazotrophic communities; NifH sequences.	
Thesagro: 	Sorghum Bicolor.	
Thesaurus Nal: 	nitrogen fixation.	
Categoria do assunto: 	P Recursos Naturais, Ciências Ambientais e da Terra	
Registro original: 	Embrapa Milho e Sorgo (CNPMS)	
Exemplares: 	CNPMS - (20970-1) - UPC - AP - DD -  -  -  - DIS