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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
07/04/2010 |
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Data da última atualização: |
07/04/2010 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
FARIAS, T. A.; ARAUJO, F. R.; OSÓRIO, A. L. A. R.; JORGE, K. S. G.; RAMOS, C. A. N.; SOUZA, I. I. F.; OLIVEIRA, R. H. M. de; AZAMBUJA, A.; ROSINHA, G. M. S.; SOARES, C. O.; ELISEI, C. |
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Afiliação: |
Thaís A. Farias, UFMS; FLABIO RIBEIRO ARAUJO, CNPGC; Ana Luíza A. R. Osório, UFMS; Kláudia S. G. Jorge, UFMS; Carlos A. N. Ramos, CNPGC; Ingrid I. F. Souza, CNPGC; RENATO HENRIQUE MARCAL DE OLIVEIRA, CNPGC; Alexandre Azambuja, UFMS; GRACIA MARIA SOARES ROSINHA, CNPGC; CLEBER OLIVEIRA SOARES, CNPGC; CARINA ELISEI, CNPGC. |
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Título: |
ELISA based on recombinant MPB70 to detect antibodies to Mycobacterium bovis. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
In: SIMPÓSIO EMBRAPA LABEX DE SANIDADE ANIMAL, 1., 2009, Campo Grande, MS. Anais... Campo Grande, MS: Embrapa Gado de Corte, 2009. |
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Páginas: |
1 p. |
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Descrição Física: |
1 CD-ROM. |
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Idioma: |
Inglês |
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Conteúdo: |
Bovine tuberculosis is an important infectious disease, caused by Mycobacterium bovis. The gene that encodes for MPB70 protein is present in the genome of M. bovis and has high specificity for the M. tuberculosis complex, representing a potential antigen for the diagnosis of bovine tuberculosis. The objective of this work was to develop an ELISA using recombinant protein MPB70 of M. bovis in order to detect antibodies against these bacteria in cattle. The gene mpb70 was amplified by PCR and cloned into plasmid pGEM-T Easy. After digestion with the Eco RI enzyme, the gene was subcloned into the expression plasmid pGEX-3X. The expression of the gene was verified by SDS-PAGE and Western blot with anti-glutathione-S-transferase. After standardization of the ELISA, serum samples of positive (53), negative (37) or inconclusive (47) cattle in the tuberculin test were tested. In SDS-PAGE, a recombinant protein of approximately 48 kDa was detected, which corresponds to the estimated molecular weight of GST/MPB70. In Western blot, recombinant MPB70, fused to GST, was recognized by anti-GST antibody, confirming the expression of the gene. For the ELISA with recombinant MPB70, the sensitivity and specificity found were 88.7% and 94.6%, respectively. The use of a serological test such as ELISA with recombinant MPB70, together with the cellular tests, may solve some of the problems found in the control programs of bovine tuberculosis, like inconclusive results and the lack of detection of anergic animals in advanced stages of the disease. MenosBovine tuberculosis is an important infectious disease, caused by Mycobacterium bovis. The gene that encodes for MPB70 protein is present in the genome of M. bovis and has high specificity for the M. tuberculosis complex, representing a potential antigen for the diagnosis of bovine tuberculosis. The objective of this work was to develop an ELISA using recombinant protein MPB70 of M. bovis in order to detect antibodies against these bacteria in cattle. The gene mpb70 was amplified by PCR and cloned into plasmid pGEM-T Easy. After digestion with the Eco RI enzyme, the gene was subcloned into the expression plasmid pGEX-3X. The expression of the gene was verified by SDS-PAGE and Western blot with anti-glutathione-S-transferase. After standardization of the ELISA, serum samples of positive (53), negative (37) or inconclusive (47) cattle in the tuberculin test were tested. In SDS-PAGE, a recombinant protein of approximately 48 kDa was detected, which corresponds to the estimated molecular weight of GST/MPB70. In Western blot, recombinant MPB70, fused to GST, was recognized by anti-GST antibody, confirming the expression of the gene. For the ELISA with recombinant MPB70, the sensitivity and specificity found were 88.7% and 94.6%, respectively. The use of a serological test such as ELISA with recombinant MPB70, together with the cellular tests, may solve some of the problems found in the control programs of bovine tuberculosis, like inconclusive results and the lack of detection of... Mostrar Tudo |
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Thesagro: |
Diagnostico; Elisa; Mycobacterium Bovis; Sanidade Animal. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02453naa a2200301 a 4500
001 1663370
005 2010-04-07
008 2009 bl uuuu u00u1 u #d
100 1 $aFARIAS, T. A.
245 $aELISA based on recombinant MPB70 to detect antibodies to Mycobacterium bovis.
260 $c2009
300 $a1 p.$c1 CD-ROM.
520 $aBovine tuberculosis is an important infectious disease, caused by Mycobacterium bovis. The gene that encodes for MPB70 protein is present in the genome of M. bovis and has high specificity for the M. tuberculosis complex, representing a potential antigen for the diagnosis of bovine tuberculosis. The objective of this work was to develop an ELISA using recombinant protein MPB70 of M. bovis in order to detect antibodies against these bacteria in cattle. The gene mpb70 was amplified by PCR and cloned into plasmid pGEM-T Easy. After digestion with the Eco RI enzyme, the gene was subcloned into the expression plasmid pGEX-3X. The expression of the gene was verified by SDS-PAGE and Western blot with anti-glutathione-S-transferase. After standardization of the ELISA, serum samples of positive (53), negative (37) or inconclusive (47) cattle in the tuberculin test were tested. In SDS-PAGE, a recombinant protein of approximately 48 kDa was detected, which corresponds to the estimated molecular weight of GST/MPB70. In Western blot, recombinant MPB70, fused to GST, was recognized by anti-GST antibody, confirming the expression of the gene. For the ELISA with recombinant MPB70, the sensitivity and specificity found were 88.7% and 94.6%, respectively. The use of a serological test such as ELISA with recombinant MPB70, together with the cellular tests, may solve some of the problems found in the control programs of bovine tuberculosis, like inconclusive results and the lack of detection of anergic animals in advanced stages of the disease.
650 $aDiagnostico
650 $aElisa
650 $aMycobacterium Bovis
650 $aSanidade Animal
700 1 $aARAUJO, F. R.
700 1 $aOSÓRIO, A. L. A. R.
700 1 $aJORGE, K. S. G.
700 1 $aRAMOS, C. A. N.
700 1 $aSOUZA, I. I. F.
700 1 $aOLIVEIRA, R. H. M. de
700 1 $aAZAMBUJA, A.
700 1 $aROSINHA, G. M. S.
700 1 $aSOARES, C. O.
700 1 $aELISEI, C.
773 $tIn: SIMPÓSIO EMBRAPA LABEX DE SANIDADE ANIMAL, 1., 2009, Campo Grande, MS. Anais... Campo Grande, MS: Embrapa Gado de Corte, 2009.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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| Registros recuperados : 39 | |
| 2. |  | PEREIRA, H. S.; BARBOSA, N. C.; CARNEIRO, M. A. C.; KORNDÖRFER, G. H. Avaliação de fontes e de extratores de silício no solo. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 42, n. 2, p. 239-247, fev. 2007 Título em inglês: Evaluation of sources and extractors of silicon in the soil.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 9. | | VILELA, L. A. F.; CARNEIRO, M. A. C.; SAGGIN JUNIOR, O. J. Fungos micorrízicos arbusculares em Latossolo Vermelho de Cerrado submetido a sucessão de cultivo. In: REUNIÃO BRASILEIRA DE CIÊNCIA DO SOLO E NUTRIÇÃO DE PLANTAS, 30.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 14.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 12.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 9.; SIMPÓSIO SOBRE SELÊNIO NO BRASIL, 1., 2012, Maceió. A responsabilidade socioambiental da pesquisa agrícola: anais. Viçosa, MG: SBCS, 2012. FERTBIO 2012.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 12. | | ASSIS, PA. C. R.; CARNEIRO, M. A. C.; SAGGIN JUNIOR, O. J. Diversidade de Fungos micorrízicos arbusculares em um plintossolo argilúvico sob cronossequências de cultivo. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 29.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 13.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 11.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 8., 2010, Guarapari. Fontes de nutrientes e produção agrícola: modelando o futuro: anais. Viçosa: SBCS, 2010.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 13. | | FERREIRA, D. A.; CARNEIRO, M. A. C.; SAGGIN JUNIOR, O. J. Densidade e diversidade de fungos micorrízicos arbusculares sob diferentes usos do solo. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 29.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 13.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 11.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 8., 2010, Guarapari. Fontes de nutrientes e produção agrícola: modelando o futuro: anais. Viçosa: SBCS, 2010.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 14. | | CARNEIRO, M. A. C.; SIQUEIRA, J. O.; CURI, N.; MOREIRA, F. M. S. Efeitos da inoculação de fungos micorrízicos arbusculares e da aplicação de fósforo no estabelecimento de forrageiras em solo degradado. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 34, n. 9, p. 1669-77, set. 1999 Título em inglês: Effects of arbuscular mycorrhizal fungi inoculation and phosphorus application on forage crops establishment in a degraded soil.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 16. | | SALES, L. R.; SILVA, G. N. da; SIQUEIRA, R. H. da S.; CARNEIRO, M. A. C.; FAQUIN, V. Arbuscular mycorrhizal fungi on the biomass and nutrition of Urochloa decumbens at different soil densities. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 53, n. 8, p. 943-951, Aug. 2018 Título em português: Influência de fungos micorrícos arbusculares na biomassa e na nutrição de Urochloa decumbens em diferentes densidades do solo.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 17. | | BARBOSA, M. V.; FUNDORA, A. B.; SILVA, A. O.; RIVERA ESPINOSA, R.; SAGGIN JUNIOR, O. J.; CARNEIRO, M. A. C. Aggregation of ferruginous nodular gleysol in a pasture area in Cuba, under the influence of arbuscular mycorrhizal fungi associated with hybrid Urochloa. Soil & Tillage Research , v. 208, 104905, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agrobiologia. |
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| Registros recuperados : 39 | |
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