Data/hora: 22/06/2024 02:03:11

Biblioteca(s): | Embrapa Cerrados.
Data corrente: | 22/12/2011
Data da última atualização: | 22/12/2011
Autoria: | MULINARI, F.; BECKER-RITT, A. B.; DEMARTINI, D. R.; LIGABUE-BRAUN, R.; STANISÇUASKI, F.; VERLI, H.; FRAGOSO, R. R.; SCHROEDER, E. K.; CARLINI, C. R.; GROSSI-de-SÁ, M. F.
Afiliação: | FERNANDA MULINARI, UFRGS; ARLETE BEATRIZ BECKER-RITT, UFRGS; DIOGO RIBEIRO DEMARTINI, UFRGS; RODRIGO LIGABUE-BRAUN, UFRGS; FERNANDA STANISÇUASKI, UFRGS; HUGO VERLI, UFRGS; RODRIGO DA ROCHA FRAGOSO, CPAC; EVELYN KOECHE SCHROEDER, UFRGS; CÉLIA REGINA CARLINI, UFRGS; MARIA FATIMA GROSSI DE SA, CENARGEN.
Título: | Characterization of JBURE-IIb isoform of Canavalia ensiformis (L.) DC urease.
Ano de publicação: | 2011
Fonte/Imprenta: | Biochimica et Biophysica Acta, v. 1814, n. 12, p. 1758-1768, Dec. 2011.
ISSN: | 1570-9639
Idioma: | Inglês

Conteúdo:

Ureases, nickel-dependent enzymes that catalyze the hydrolysis of urea into ammonia and bicarbonate, are
widespread in plants, bacteria, and fungi. Previously, we cloned a cDNA encoding a Canavalia ensiformis urease
isoform named JBURE-II, corresponding to a putative smaller urease protein (78 kDa) when compared to
other plant ureases. Aiming to produce the recombinant protein, we obtained jbure-IIb, with different 3? and
5? ends, encoding a 90 kDa urease. Three peptides unique to the JBURE-II/-IIb protein were detected by mass
spectrometry in seed extracts, indicating that jbure-II/-IIb is a functional gene. Comparative modeling indicates
that JBURE-IIb urease has an overall shape almost identical to C. ensiformis major urease JBURE-I with
all residues critical for urease activity. The cDNA was cloned into the pET101 vector and the recombinant protein
was produced in Escherichia coli. The JBURE-IIb protein, although enzymatically inactive presumably due
to the absence of Ni atoms in its active site, impaired the growth of a phytopathogenic fungus and showed
entomotoxic properties, inhibiting diuresis of Rhodnius prolixus isolated Malpighian tubules, in concentrations
similar to those reported for JBURE-I and canatoxin. The antifungal and entomotoxic properties of the
recombinant JBURE-IIb apourease are consistent with a protective role of ureases in plants.

Thesagro: | Canavalia Ensiformis.
Categoria do assunto: | --
Registro original: | Embrapa Cerrados (CPAC)
Exemplares: | CPAC - (32982-1) - UPC - SP - PP - S1465 -  - S1465 - DIS