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2. | | CARDOZO FILHO, J. de L.; MONTEIRO, R. do N.; SILVA, L. P. da; BLOCH JUNIOR, C.; STÁBELI, R. G.; CALDERON, L. de A. Identification of a phylloseptin from Amazon Phyllomedusa palliata(Anura: Hylidae) by Maldi TOT/TOF MS. In: CONGRESSO DA SOCIEDADE BRASILEIRA DE TOXINOLOGIA, 11., 2010, Araxá. Toxinas naturais: conhecimento atual e novos desafios. [S.l.]: Sociedade Brasileira de Toxinologia, 2010. Pôster. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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3. | | SOUSA, A. M. P.; SALLES, H. O.; OLIVEIRA, H. D. de; SOUZA, B. B. P. de; CARDOZO FILHO, J. de L.; SIFUENTES, D. N.; PRATES, M. V.; BLOCH JUNIOR, C.; BEMQUERER, M. P.; EGITO, A. S. do. Mo-HLPs: New flocculating agents identified from Moringa oleifera seeds belong to the hevein-like peptide family. Journal of Proteomics, v. 217, article 103692, Apr. 2020. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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4. | | SOUSA, A. M. P.; SALLES, H. O.; OLIVEIRA, H. D. de; SOUZA, B. B. P. de; CARDOZO FILHO, J. de L.; SIFUENTES, D. N.; PRATES, M. V.; BLOCH JUNIOR, C.; BEMQUERER, M. P.; VASCONCELOS, A. S. do E. Mo-HLPs: new flocculating agents identified from Moringa oleifera seeds belong to the hevein-like peptide family. Journal of Proteomics, v. 217, article 103692, Apr. 2020. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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5. | | CARDOZO FILHO, J. de L.; SOARES, A. A.; SILVA, L. P. da; MONTEIRO, J. R. do N.; ALVES, S. C. de L.; SILVA, R. S.; KAYANO, A. M.; BLOCH JUNIOR, C.; STÁBELI, R. G.; CALDERON, L. de A. Identification of peptides from Amazon Leptodactylus knudseni (Anura: Leptodactylidae) by Maldi TOF/TOF MS. In: CONGRESSO DA SOCIEDADE BRASILEIRA DE TOXINOLOGIA, 11., 2010, Araxá. Toxinas naturais: conhecimento atual e novos desafios. [S.l.]: Sociedade Brasileira de Toxinologia, 2010. Pôster. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
30/04/2020 |
Data da última atualização: |
06/08/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
SOUSA, A. M. P.; SALLES, H. O.; OLIVEIRA, H. D. de; SOUZA, B. B. P. de; CARDOZO FILHO, J. de L.; SIFUENTES, D. N.; PRATES, M. V.; BLOCH JUNIOR, C.; BEMQUERER, M. P.; EGITO, A. S. do. |
Afiliação: |
ANA MARJORY PAIVA SOUSA, Rede Nordeste de Biotecnologia-RENORBIO, Universidade Estadual do Ceará (UECe) - Fortaleza, CE, Brazil; HEVILA OLIVEIRA SALLES, CNPC; HERMOGENES DAVID DE OLIVEIRA, Universidade Federal do Ceará (UFC) - Fortaleza, CE, Brazil; BEATRIZ BLENDA PINHEIRO DE SOUZA; JOSE DE LIMA CARDOZO FILHO; DANIEL NOGOCEKE SIFUENTES, Cenargen; MAURA VIANNA PRATES, Cenargen; CARLOS BLOCH JUNIOR, Cenargen; MARCELO PORTO BEMQUERER, Cenargen; ANTONIO SILVIO DO EGITO, CNPC. |
Título: |
Mo-HLPs: New flocculating agents identified from Moringa oleifera seeds belong to the hevein-like peptide family. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Journal of Proteomics, v. 217, article 103692, Apr. 2020. |
DOI: |
https://doi.org/10.1016/j.jprot.2020.103692 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: Cationic peptides found in Moringa oleifera seeds belong to different protein families and are described as the main flocculating agents of the species. In this study we report the identification and isolation of four new flocculant peptides, called Mo-HLPs 1?4, belonging to the family of hevein-like peptides, previously only known for their members' antimicrobial activity. Purification of the peptides followed two sequential membrane ultrafiltration steps and separation by reverse-phase liquid chromatography. Proteomic analyses showed that Mo-HLPs are extremely basic (pI >10) cysteine-rich molecules with molecular masses between 4.5 and 4.8 kDa and with a highly conserved chitin-binding domain. Searches in BLAST revealed high similarity of Mo-HLPs with hevein and other hevein-like peptides and 90% identity with morintides, which are members of the 8C-hevein-like subfamily found in M. oleifera leaves. Mo-HLPs microflocculation assays showed distinct coagulation/flocculation efficiencies, promoting turbidity reduction levels between 67 and 89% in synthetic turbid water. Activity variations were attributed to the substitution of some amino acids among the isoforms, which may have altered the final net charge of the molecules. The identification of Mo-HLPs represents the discovery of a new group of cationic peptides involved in the flocculation properties of M. oleifera seeds. |
Palavras-Chave: |
Cationic peptides; Flocculant activity; Hevein-like. |
Thesagro: |
Moringa Oleifera. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02324naa a2200289 a 4500 001 2121962 005 2020-08-06 008 2020 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.jprot.2020.103692$2DOI 100 1 $aSOUSA, A. M. P. 245 $aMo-HLPs$bNew flocculating agents identified from Moringa oleifera seeds belong to the hevein-like peptide family.$h[electronic resource] 260 $c2020 520 $aAbstract: Cationic peptides found in Moringa oleifera seeds belong to different protein families and are described as the main flocculating agents of the species. In this study we report the identification and isolation of four new flocculant peptides, called Mo-HLPs 1?4, belonging to the family of hevein-like peptides, previously only known for their members' antimicrobial activity. Purification of the peptides followed two sequential membrane ultrafiltration steps and separation by reverse-phase liquid chromatography. Proteomic analyses showed that Mo-HLPs are extremely basic (pI >10) cysteine-rich molecules with molecular masses between 4.5 and 4.8 kDa and with a highly conserved chitin-binding domain. Searches in BLAST revealed high similarity of Mo-HLPs with hevein and other hevein-like peptides and 90% identity with morintides, which are members of the 8C-hevein-like subfamily found in M. oleifera leaves. Mo-HLPs microflocculation assays showed distinct coagulation/flocculation efficiencies, promoting turbidity reduction levels between 67 and 89% in synthetic turbid water. Activity variations were attributed to the substitution of some amino acids among the isoforms, which may have altered the final net charge of the molecules. The identification of Mo-HLPs represents the discovery of a new group of cationic peptides involved in the flocculation properties of M. oleifera seeds. 650 $aMoringa Oleifera 653 $aCationic peptides 653 $aFlocculant activity 653 $aHevein-like 700 1 $aSALLES, H. O. 700 1 $aOLIVEIRA, H. D. de 700 1 $aSOUZA, B. B. P. de 700 1 $aCARDOZO FILHO, J. de L. 700 1 $aSIFUENTES, D. N. 700 1 $aPRATES, M. V. 700 1 $aBLOCH JUNIOR, C. 700 1 $aBEMQUERER, M. P. 700 1 $aEGITO, A. S. do 773 $tJournal of Proteomics$gv. 217, article 103692, Apr. 2020.
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