|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
13/02/2015 |
Data da última atualização: |
23/05/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
FERREIRA, D. A. F. da; COTTA, S. R.; VOLLÚ, R. E.; JURELEVICIUS, D. A. de; MARQUES, J. M.; MARRIEL, I. E.; SELDIN, L. |
Afiliação: |
IVANILDO EVODIO MARRIEL, CNPMS. |
Título: |
Endophytic microbial community in two transgenic maize genotypes and in their near-isogenic non-transgenic maize genotype. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
BMC Microbiology, v. 14, n. 332, p. 1-9, 2014. |
Idioma: |
Inglês |
Conteúdo: |
Background: Despite all the benefits assigned to the genetically modified plants, there are still no sufficient data available in literature concerning the possible effects on the microbial communities associated with these plants. Therefore, this study was aimed at examining the effects of the genetic modifications of two transgenic maize genotypes (MON810–expressing the insecticidal Bt-toxin and TC1507–expressing the insecticidal Bt-toxin and the herbicide resistance PAT [phosphinothricin-N -acetyltransferase]) on their endophytic microbial communities, in comparison to the microbial community found in the near-isogenic non-transgenic maize (control). Results: The structure of the endophytic communities (Bacteria, Archaea and fungi) and their composition (Bacteria) were evaluated by denaturing gradient gel electrophoresis (DGGE) and the construction of clone libraries, respectively. DGGE analysis and the clone libraries of the bacterial community showed that genotype TC1507 slightly differed from the other two genotypes. Genotype TC1507 showed a higher diversity within its endophytic bacterial community when compared to the other genotypes. Although some bacterial genera were found in all genotypes, such as the genera Burkholderia, Achromobacer and Stenotrophomonas, some were unique to genotype TC1507. Moreover, OTUs associated with Enterobacter predominated only in TC1507 clone libraries. Conclusion: The endophytic bacterial community of the maize genotype TC1507 differed from the communities of the maize genotype MON810 and of their near-isogenic parental genotypes (non-Bt or control). The differences observed among the maize genotypes studied may be associated with insertion of the gene coding for the protein PAT present only in the transgenic genotype TC1507. MenosBackground: Despite all the benefits assigned to the genetically modified plants, there are still no sufficient data available in literature concerning the possible effects on the microbial communities associated with these plants. Therefore, this study was aimed at examining the effects of the genetic modifications of two transgenic maize genotypes (MON810–expressing the insecticidal Bt-toxin and TC1507–expressing the insecticidal Bt-toxin and the herbicide resistance PAT [phosphinothricin-N -acetyltransferase]) on their endophytic microbial communities, in comparison to the microbial community found in the near-isogenic non-transgenic maize (control). Results: The structure of the endophytic communities (Bacteria, Archaea and fungi) and their composition (Bacteria) were evaluated by denaturing gradient gel electrophoresis (DGGE) and the construction of clone libraries, respectively. DGGE analysis and the clone libraries of the bacterial community showed that genotype TC1507 slightly differed from the other two genotypes. Genotype TC1507 showed a higher diversity within its endophytic bacterial community when compared to the other genotypes. Although some bacterial genera were found in all genotypes, such as the genera Burkholderia, Achromobacer and Stenotrophomonas, some were unique to genotype TC1507. Moreover, OTUs associated with Enterobacter predominated only in TC1507 clone libraries. Conclusion: The endophytic bacterial community of the maize genotype TC1507 differed... Mostrar Tudo |
Palavras-Chave: |
Enzima PAT; Proteína Cry. |
Thesagro: |
Alimento transgênico; Milho. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02526naa a2200241 a 4500 001 2008692 005 2017-05-23 008 2014 bl uuuu u00u1 u #d 100 1 $aFERREIRA, D. A. F. da 245 $aEndophytic microbial community in two transgenic maize genotypes and in their near-isogenic non-transgenic maize genotype.$h[electronic resource] 260 $c2014 520 $aBackground: Despite all the benefits assigned to the genetically modified plants, there are still no sufficient data available in literature concerning the possible effects on the microbial communities associated with these plants. Therefore, this study was aimed at examining the effects of the genetic modifications of two transgenic maize genotypes (MON810–expressing the insecticidal Bt-toxin and TC1507–expressing the insecticidal Bt-toxin and the herbicide resistance PAT [phosphinothricin-N -acetyltransferase]) on their endophytic microbial communities, in comparison to the microbial community found in the near-isogenic non-transgenic maize (control). Results: The structure of the endophytic communities (Bacteria, Archaea and fungi) and their composition (Bacteria) were evaluated by denaturing gradient gel electrophoresis (DGGE) and the construction of clone libraries, respectively. DGGE analysis and the clone libraries of the bacterial community showed that genotype TC1507 slightly differed from the other two genotypes. Genotype TC1507 showed a higher diversity within its endophytic bacterial community when compared to the other genotypes. Although some bacterial genera were found in all genotypes, such as the genera Burkholderia, Achromobacer and Stenotrophomonas, some were unique to genotype TC1507. Moreover, OTUs associated with Enterobacter predominated only in TC1507 clone libraries. Conclusion: The endophytic bacterial community of the maize genotype TC1507 differed from the communities of the maize genotype MON810 and of their near-isogenic parental genotypes (non-Bt or control). The differences observed among the maize genotypes studied may be associated with insertion of the gene coding for the protein PAT present only in the transgenic genotype TC1507. 650 $aAlimento transgênico 650 $aMilho 653 $aEnzima PAT 653 $aProteína Cry 700 1 $aCOTTA, S. R. 700 1 $aVOLLÚ, R. E. 700 1 $aJURELEVICIUS, D. A. de 700 1 $aMARQUES, J. M. 700 1 $aMARRIEL, I. E. 700 1 $aSELDIN, L. 773 $tBMC Microbiology$gv. 14, n. 332, p. 1-9, 2014.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
Registro Completo
Biblioteca(s): |
Embrapa Agroindústria Tropical. |
Data corrente: |
30/10/2009 |
Data da última atualização: |
09/06/2017 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
CAVALCANTI, J. J. V.; BARROS, L. de M.; LIMA, A. C.; MESQUITA, A. L. M.; CARDOSO, J. E. |
Afiliação: |
José Jaime Vasconcelos Cavalcante, CNPAT; Levi de Moura Barros, CNPAT; Antônio Calixto de Lima, CNPAT; Antônio Lindemberg Martins Mesquita, CNPAT; José Emilson Cardoso, CNPAT. |
Título: |
Clones de cajueiro. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
In: SEMINÁRIO DA INOVAÇÃO TECNOLÓGICA NO NORDESTE, 5., 2009, Fortaleza. Caderno do Participante. Fortaleza: Instituto de Desenvolvimento Industrial do Ceará, 2009. p.21. |
Descrição Física: |
Co-autores CNPAT: BARROS, L. de M.; LIMA, A. C.; MESQUITA, A. L. M.; CARDOSO, J. E. |
Idioma: |
Português |
Palavras-Chave: |
Cajueiro. |
Thesagro: |
Caju; Clone; Melhoramento; Propagação Vegetativa; Seleção. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CNPAT-2010/11381/1/RE09026.pdf
|
Marc: |
LEADER 00782nam a2200229 a 4500 001 1573789 005 2017-06-09 008 2009 bl uuuu u00u1 u #d 100 1 $aCAVALCANTI, J. J. V. 245 $aClones de cajueiro. 260 $aIn: SEMINÁRIO DA INOVAÇÃO TECNOLÓGICA NO NORDESTE, 5., 2009, Fortaleza. Caderno do Participante. Fortaleza: Instituto de Desenvolvimento Industrial do Ceará, 2009. p.21.$c2009 300 $cCo-autores CNPAT: BARROS, L. de M.; LIMA, A. C.; MESQUITA, A. L. M.; CARDOSO, J. E. 650 $aCaju 650 $aClone 650 $aMelhoramento 650 $aPropagação Vegetativa 650 $aSeleção 653 $aCajueiro 700 1 $aBARROS, L. de M. 700 1 $aLIMA, A. C. 700 1 $aMESQUITA, A. L. M. 700 1 $aCARDOSO, J. E.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Agroindústria Tropical (CNPAT) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|