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| Registros recuperados : 57 | |
| 41. |  | LEAO, A. P.; VARGAS, L. H. G.; FARIAS, M. P.; FORMIGHIERI, E. F.; ALVES, A. A.; CAPDEVILLE, G. de; SOUZA JUNIOR, M. T. Estabelecimento de curva de melting para elaeis guineenses jacq. e elaeis oleífera (kunth) cortés. In: WORKSHOP AGROENERGIA, 6., 2012, Ribeirão Preto, SP. Anais... Ribeirão Preto: APTA; Campinas: IAC, 2012. Não paginado| Biblioteca(s): Embrapa Agroenergia. |
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| 42. | | SOUZA JUNIOR, M. T.; CAPDEVILLE, G. de; ONOYAMA, M. M.; SOUZA, D. T. de; SANTOS, G. S.; ALMEIDA, J. R. M. de; LIMA, R. S.; GAMBETTA, R. Biodiesel e bioquerosene: o papel da Embrapa Agroenergia. Brasília, DF: Embrapa Agroenergia, 2017. 34 p. (Embrapa Agroenergia. Documentos, 21).| Biblioteca(s): Embrapa Agroenergia. |
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| 44. | | FARIAS, M. P.; VARGAS, L. H. G.; LEAO, A. P.; FORMIGHIERI, E. F.; ALVES, A. A.; CAPDEVILLE, G. de; SOUZA JUNIOR, M. T. Determinação de estádios meióticos em elaeis oleifera (kunth) cortés. In: WORKSHOP AGROENERGIA, 6., 2012, Ribeirão Preto, SP. Anais... Ribeirão Preto: APTA; Campinas: IAC, 2012. Não paginado| Biblioteca(s): Embrapa Agroenergia. |
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| 45. | | ALVES, A. A.; FORMIGHIERI, E. F.; LEAO, A. P.; LOBO, F. P.; RIOS, S. A.; CAPDEVILLE, G. de; SOUZA JUNIOR, M. T. Exploring American Oil Palm (Elaeis oleifera) genetic resources in Brazil through a combination of genetic and genomic approaches. In: PLANT & ANIMAL GENOME CONFERENCE, 22., 2014, San Diego, CA. [Abstracts]. San Diego: [s.n.], 2014. Não paginado. Pôster W551.| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Agroenergia. |
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| 46. | | DIAS, B. B. A.; CUNHA, W. G.; MORAIS, L. S.; VIANNA, G. R.; RECH, E. L.; CAPDEVILLE, G. de; ARAGÃO, F. J. L. Expression of an oxalate decarboxylase gene from Flammulina sp. in transgenic lettuce (Lactuca sativa) plants and resistance to Sclerotinia sclerotiorum. Plant Pathology, v. 55, p. 187-193, 2006.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 47. | | FERNANDES, M. S.; MORAIS, P. B.; NASCIMENTO, R. C. do; GARCIA, L. C.; CAPDEVILLE, G. de; BRASIL, B. dos S. A. F. Efeito da morfologia celular e fase de crescimento algal na eficiência da criopreservação de microalgas. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 3., 2016, Brasília, DF. Anais ... Brasília, DF: Embrapa, 2016. p. 95-101.| Biblioteca(s): Embrapa Agroenergia. |
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| 48. | | AMARAL, P. de P. R.; ALVES, P. C. M.; MARTINS, N. F.; SILVA, F. R. da; CAPDEVILLE, G. de; SOUZA JÚNIOR, M. T. Identification and characterization of a resistance gene analog (RGA) from the Caricaceae Dumort family. Revista Brasileira de Fruticultura, v. 28, n. 3, p. 458-462, dez. 2006.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 49. | | AMARAL, P. de P. R.; ALVES, P. C. M.; MARTINS, N. F.; SILVA, F. R. da; CAPDEVILLE, G. de; SOUZA JÚNIOR, M. T. Identification and characterization of a Resistence Gene Analog (RGA) from the Caricaceae Dumort family. Revista Brasileira de Fruticultura, Jaboticabal, v. 28, n. 3, p. 458-462, dez. 2006.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 51. | | CAMILLO, J.; LEAO, A. P.; ALVES, A. A.; FORMIGHIERI, E. F.; AZEVEDO, A. L. S.; NUNES, J. D.; CAPDEVILLE, G. de; MATTOS, J. K. de A.; SOUZA JUNIOR, M. T. Reassessment of the genome size in elaeis guineensis and elaeis oleifera, and its interspecific hybrid. Genomics Insights, v. 7, p. 13-22, 2014.| Biblioteca(s): Embrapa Agroenergia; Embrapa Gado de Leite. |
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| 52. | | FARIAS, M. P. de; CAPDEVILLE, G. de; FALCAO, R.; MORAES, P. B. de; LEAO, A. P.; CAMILLO, J.; CUNHA, R. N. V. da; ALVES, A. A.; SOUZA JUNIOR, M. T. Microscopic characterization of American oil palm (Elaeis oleifera (Kunth) Cortés) floral development. Flora, v. 243, p. 88-100, 2018.| Biblioteca(s): Embrapa Agroenergia; Embrapa Amazônia Ocidental. |
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| 53. | | PEREIRA, V. M.; LEAO, A. P.; VARGAS, L. H.; FARIAS, M. P. de; FORMIGHERI, E. F.; CAPDEVILLE, G. de; SOUZA JUNIOR, M. T.; RIOS, S. de A.; ALVES, A. A. Extensão e organização da diversidade genética da coleção brasileira de germoplasma de Elaeis oleifera. In: CONGRESSO INTERNACIONAL DE BIOENERGIA, 8., 2013, São Paulo, SP. Anais... São Paulo: CIPA, 2013.| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 54. | | PEREIRA, V. M.; LEAO, A. P.; VARGAS, L. H.; FARIAS, M. P. de; FORMIGHIERI, E. F.; CAPDEVILLE, G. de; SOUZA JUNIOR, M. T.; RIOS, S. de A.; ALVES, A. A. Extensão e organização da diversidade genética da coleção brasileira de germoplasma de Elaeis oleifera. In: CONGRESSO INTERNACIONAL DE BIOENERGIA, 8., 2013, São Paulo, SP. Anais... São Paulo: CIPA, 2013.| Biblioteca(s): Embrapa Agroenergia. |
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| 55. | | GOMES, A. C. M. M.; NICOLE, M.; MATTOS, J. K.; PEREIRA, S. I. V.; PEREIRA, P.; SILVA, D. B. da; VIEIRA, R. F.; CAPDEVILLE, G. de; CARNEIRO, R. M. D. G. Concentration of 20E on susceptible and resistant accessions of Pfaffia glomerata infected with Meloidogyne incognita and histological characterization of resistance. In: INTERNATIONAL CONGRESS OF TROPICAL NEMATOLOGY, 2., 2009, Maceió. Abstracts... Maceió: ONTA: SBN, 2009. 1 CD-ROM| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 56. | | GOMES, A. C. M. M.; NICOLE, M.; MATTOS, J. K.; PEREIRA, S. I. V.; PEREIRA, P.; SILVA, D. B. da; VIEIRA, R. F.; CAPDEVILLE, G. de; MOITA, A. W.; CARNEIRO, R. M. D. G. Concentration of B-ecydisone (20E) in susceptible and resistant accessions of Pfaffia glomerata infected with Meloidogyne incognita and histological characterisation of resistance. Nematology, Leiden, v. 12, n. 5, p. 701-709, 2010.| Biblioteca(s): Embrapa Hortaliças; Embrapa Recursos Genéticos e Biotecnologia. |
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| 57. | | MOREL, J. B.; FERREIRA, M.; CAPDEVILLE, G. de; SCAGLIUSI, S. M. M.; BONATO, A. L. V.; MEHTA, Y.; MACIEL, J. L. N.; SCHEEREN, P. L.; CHAVES, M. S.; BRAMMER, S. P.; RONALD, P.; JUNG, K. H.; SING, R.; WILLIAM, M.; KIKUCHI, S.; SATOH, K.; BOYD, L.; TUFAN, H.; MCGRANN, G.; FEUILLET, C.; SOURDILLE, P.; SALSE, J.; MICHÉ, L.; BOUSQUET, M.; MICHEL, C. Functional genomics of cross-species resistance to fungal diseases in rice and wheat (CEREALIMMUNITY). In: GENERATION CHALLENGE PROGRAMME CULTIVATING PLANT DIVERSITY FOR THE RESOURCE-POOR, 2008, Mexico. Project abstracts... Mexico: Generation Challenge programme, 2008. p. 19-21.| Biblioteca(s): Embrapa Trigo. |
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| Registros recuperados : 57 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com cnpae.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
15/03/2019 |
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Data da última atualização: |
18/11/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
FERNANDES, M. S.; GARCIA, L. C.; NASCIMENTO, R. C.; SANTANA, H.; MORAIS, P. B.; CAPDEVILLE, G. de; BRASIL, B. dos S. A. F. |
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Afiliação: |
Maiara S. Fernandes; LORENA COSTA GARCIA CALSING, CNPAE; Rodrigo C. Nascimento; Hugo Santana; Priscila B. Morais; GUY DE CAPDEVILLE, CNPAE; BRUNO DOS SANTOS A F BRASIL, SPD. |
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Título: |
Customized cryopreservation protocols for chlorophytes based on cell morphology. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Algal Research, v. 38, 2019. |
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Idioma: |
Português |
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Conteúdo: |
The development of efficient algae cryopreservation methods is pivotal to the establishment of long-term culture collections as well as algae breeding and genetic modification programs. However, the unpredictable responses of distinct algal species to cryopreservation agents and protocols have hampered the standardization of universal methods so far. The results presented in this study indicate that intrinsic biological factors (i.e.: cell morphology and phylogenetic origin) play a role in the definition of which culture age and cryoprotectant agent type and concentration should be used to achieve successful cryopreservation. Through the use of Central Composite Rotatable Design (CCRD) it was possible to define optimized protocols for cryopreserving three major morphotypic groups found among chlorophytes (i.e.: coenobium, coccoid or palmella forming strains). These optimized protocols were then validated upon fifteen strains from Sphaeropleales, Chlamydomonadales and Chlorelalles clades. Analysis reveals that the use of DMSO as the sole cryoprotectant provided the highest postfreezing cell viability recovery rates for 80% of the coccoid strains tested, while the combination of glycerol and PEG400 allowed efficient cryopreservation of 100% of the coenobium and palmella forming strains evaluated. In addition, results suggest that coenobium-forming strains have higher resistance to freeze/thawing when frozen at mid-log phase of growth, while coccoid and plamelloid strains present higher survival rates when cryopreserved at late-log phase. Such information is of valuable practical use since a simple visual inspection of algae strain's predominant morphotype can guide the choice of the most suitable cryopreservation protocol. MenosThe development of efficient algae cryopreservation methods is pivotal to the establishment of long-term culture collections as well as algae breeding and genetic modification programs. However, the unpredictable responses of distinct algal species to cryopreservation agents and protocols have hampered the standardization of universal methods so far. The results presented in this study indicate that intrinsic biological factors (i.e.: cell morphology and phylogenetic origin) play a role in the definition of which culture age and cryoprotectant agent type and concentration should be used to achieve successful cryopreservation. Through the use of Central Composite Rotatable Design (CCRD) it was possible to define optimized protocols for cryopreserving three major morphotypic groups found among chlorophytes (i.e.: coenobium, coccoid or palmella forming strains). These optimized protocols were then validated upon fifteen strains from Sphaeropleales, Chlamydomonadales and Chlorelalles clades. Analysis reveals that the use of DMSO as the sole cryoprotectant provided the highest postfreezing cell viability recovery rates for 80% of the coccoid strains tested, while the combination of glycerol and PEG400 allowed efficient cryopreservation of 100% of the coenobium and palmella forming strains evaluated. In addition, results suggest that coenobium-forming strains have higher resistance to freeze/thawing when frozen at mid-log phase of growth, while coccoid and plamelloid strains prese... Mostrar Tudo |
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Palavras-Chave: |
Central composite rotational design; Morphotype. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02383naa a2200217 a 4500
001 2107118
005 2019-11-18
008 2019 bl uuuu u00u1 u #d
100 1 $aFERNANDES, M. S.
245 $aCustomized cryopreservation protocols for chlorophytes based on cell morphology.$h[electronic resource]
260 $c2019
520 $aThe development of efficient algae cryopreservation methods is pivotal to the establishment of long-term culture collections as well as algae breeding and genetic modification programs. However, the unpredictable responses of distinct algal species to cryopreservation agents and protocols have hampered the standardization of universal methods so far. The results presented in this study indicate that intrinsic biological factors (i.e.: cell morphology and phylogenetic origin) play a role in the definition of which culture age and cryoprotectant agent type and concentration should be used to achieve successful cryopreservation. Through the use of Central Composite Rotatable Design (CCRD) it was possible to define optimized protocols for cryopreserving three major morphotypic groups found among chlorophytes (i.e.: coenobium, coccoid or palmella forming strains). These optimized protocols were then validated upon fifteen strains from Sphaeropleales, Chlamydomonadales and Chlorelalles clades. Analysis reveals that the use of DMSO as the sole cryoprotectant provided the highest postfreezing cell viability recovery rates for 80% of the coccoid strains tested, while the combination of glycerol and PEG400 allowed efficient cryopreservation of 100% of the coenobium and palmella forming strains evaluated. In addition, results suggest that coenobium-forming strains have higher resistance to freeze/thawing when frozen at mid-log phase of growth, while coccoid and plamelloid strains present higher survival rates when cryopreserved at late-log phase. Such information is of valuable practical use since a simple visual inspection of algae strain's predominant morphotype can guide the choice of the most suitable cryopreservation protocol.
653 $aCentral composite rotational design
653 $aMorphotype
700 1 $aGARCIA, L. C.
700 1 $aNASCIMENTO, R. C.
700 1 $aSANTANA, H.
700 1 $aMORAIS, P. B.
700 1 $aCAPDEVILLE, G. de
700 1 $aBRASIL, B. dos S. A. F.
773 $tAlgal Research$gv. 38, 2019.
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