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Registros recuperados : 148 | |
5. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | SILVA, M. L. B. da; CANTAO, M. E.; MEZZARI, M. P.; MA, J.; NOSSA, C. W. Assessment of bacterial and archaeal community structure in swine wastewater treatment processes. In: SIMPÓSIO INTERNACIONAL SOBRE GERENCIAMENTO DE RESÍDUOS AGROPECUÁRIOS E AGROINDUSTRIAIS, 4., 2015, Rio de Janeiro, RJ. Anais... Brasília: Embrapa, 2015. Biblioteca(s): Embrapa Suínos e Aves. |
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7. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | PEIXOTO, J. de O.; IBELLI, A. M. G.; CANTAO, M. E.; LEDUR, M. C. Condronecrose bacteriana com osteomielite (BCO): um problema subestimado na avicultura de corte. Guia Gessulli da Avicultura e Suinocultura Industrial, Itu, ed. 266, ano 38, n. 5, p. 24-30, 2015. Biblioteca(s): Embrapa Suínos e Aves. |
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8. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | SCHAEFER, R.; GAVA, D.; HAACH, V.; CANTAO, M. E.; ZANELLA, J. R. C. Continuous circulation of human-origin seasonal influenza A viruses in swine in Brazil. Virus Reviews & Research, Brasília, DF, v. 20, p. 242, Oct. 2015. Supplement 1, ref. VV301. Edição dos Resumos do XXVI Brazilian Congress of Virology, X Mercosur Meeting of Virology, Florianóplis, SC, Oct. 2015. Biblioteca(s): Embrapa Suínos e Aves. |
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9. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | MEZZARI, M. P.; SILVA, M. L. B. da; CANTAO, M. E.; MA, J.; NOSSA, C. W. Insights of putative pathogens reduction from swine wastewater treatment processes through 16s rDNA pyrosequencing analyses. In: SIMPÓSIO INTERNACIONAL SOBRE GERENCIAMENTO DE RESÍDUOS AGROPECUÁRIOS E AGROINDUSTRIAIS, 4., 2015, Rio de Janeiro, RJ. Anais... Brasília: Embrapa, 2015. Biblioteca(s): Embrapa Suínos e Aves. |
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10. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | NELSON, M. I.; SCHAEFER, R.; GAVA, D.; CANTAO, M. E.; ZANELLA, J. R. C. Influenza A viruses of human origin in swine, Brazil. Emerging Infectious Diseases, v. 21, n. 8, p. 1339 - 1347, 2015. Biblioteca(s): Embrapa Suínos e Aves. |
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11. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | ZANELLA, J. R. C.; SCHAEFER, R.; GAVA, D.; HAACH, V.; CANTAO, M. E.; COLDEBELLA, A. Influenza A virus infection in Brazilian swine herds following the introduction of pandemic 2009 H1N1. Veterinary Microbiology, v. 180, n. 1-2, p. 118-122, 2015. Biblioteca(s): Embrapa Suínos e Aves. |
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12. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | SCHAEFER, R.; MORES, N.; GAVA, D.; CANTAO, M. E.; ZANELLA, J. R. C. Long-term circulation of influenza A viruses in swine in Brazil. In: Journal of the Brazilian Society for Virology, Ribeirão Preto, v. 19, p. 53-54, 2014. Suppl. 2. Edição dos anais do 25º. Brazilian Congress of Virology e 9º. Mercosur Meeting of Virology, Ribeirão Preto, set./out, 2014. Biblioteca(s): Embrapa Suínos e Aves. |
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20. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | SCHAEFER, R.; GAVA, D.; HAACH, V.; ZANELLA, J. R. C.; CANTAO, M. E. Novel H1N2 swine influenza virus reassortant strain derived from the pandemic H1N1/2009 virus. In: CONGRESSO BRASILEIRO DE VIROLOGIA, 29.; ENCONTRO DE VIROLOGIA DO MERCOSUL, 13., 2018, Gramado. Resumos... Brasília: SBV, 2018. Biblioteca(s): Embrapa Suínos e Aves. |
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Registros recuperados : 148 | |
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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Suínos e Aves. |
Data corrente: |
01/11/2019 |
Data da última atualização: |
01/11/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
HAACH, V.; GAVA, D.; CANTAO, M. E.; FRANCO, A. C. |
Afiliação: |
VANESSA HAACH, UFRGS; DANIELLE GAVA, CNPSA; MAURICIO EGIDIO CANTAO, CNPSA; ANA CLÁUDIA FRANCO, UFRGS. |
Título: |
One-step multiplex RT-qPCR for the detection and subtyping of influenza A virus in swine in Brazil. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Journal of Virological Methods, v. 269, p. 43-48, 2019. |
DOI: |
10.1016/j.jviromet.2019.04.005 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: Pandemic H1N1, human-like H1N2 and H3N2 influenza A (IAV) viruses are co-circulating in swine herds in Brazil. The genetic analysis of the Brazilian IAVs has shown that they are genetically distinct from viruses found in swine in other countries; therefore, an update of the diagnostic assays for IAV detection and subtyping is needed. This study describes the development and validation of a TaqMan based ? one-step multiplex RT-qPCR to discriminate the hemagglutinin and neuraminidase genes of the three major IAV ubtypes circulating in pigs in Brazil. The RT-qPCR assays presented 100% (95.7?100, CI 95%) of diagnostic sensitivity in the analysis of 85 IAVs, previously characterized by sequencing. The limits of detection ranged from 5.09 × 101 to 5.09 × 103 viral RNA copies/μL. For the analytical specificity, 73 pig samples collected during 2017 and 2018 were analyzed, resulting in the identification of the subtype in 74.0% (62.9?82.7, CI 95%) of samples. From these, 46.3% were H3N2, 33.3% were H1N1, 11.1% were H1N2 and 3.7% were HxN1. Mixed viral infections (3.7%) and reassortant viruses (1.9%) were also detected by the test. This multiplex RT-qPCR assay provides a fast and specific diagnostic tool for identification of different subtypes and lineages of IAV in pigs, contributing to the monitoring of influenza in swine. |
Palavras-Chave: |
Diagnóstico rápido; Multiplex RT-qPCR; Rapid diagnosis; RT-qPCR multiplex; Subtipagem; Subtyping; Vírus da gripe A. |
Thesagro: |
Suíno. |
Thesaurus NAL: |
Influenza A virus; Swine. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02191naa a2200289 a 4500 001 2113827 005 2019-11-01 008 2019 bl uuuu u00u1 u #d 024 7 $a10.1016/j.jviromet.2019.04.005$2DOI 100 1 $aHAACH, V. 245 $aOne-step multiplex RT-qPCR for the detection and subtyping of influenza A virus in swine in Brazil.$h[electronic resource] 260 $c2019 520 $aAbstract: Pandemic H1N1, human-like H1N2 and H3N2 influenza A (IAV) viruses are co-circulating in swine herds in Brazil. The genetic analysis of the Brazilian IAVs has shown that they are genetically distinct from viruses found in swine in other countries; therefore, an update of the diagnostic assays for IAV detection and subtyping is needed. This study describes the development and validation of a TaqMan based ? one-step multiplex RT-qPCR to discriminate the hemagglutinin and neuraminidase genes of the three major IAV ubtypes circulating in pigs in Brazil. The RT-qPCR assays presented 100% (95.7?100, CI 95%) of diagnostic sensitivity in the analysis of 85 IAVs, previously characterized by sequencing. The limits of detection ranged from 5.09 × 101 to 5.09 × 103 viral RNA copies/μL. For the analytical specificity, 73 pig samples collected during 2017 and 2018 were analyzed, resulting in the identification of the subtype in 74.0% (62.9?82.7, CI 95%) of samples. From these, 46.3% were H3N2, 33.3% were H1N1, 11.1% were H1N2 and 3.7% were HxN1. Mixed viral infections (3.7%) and reassortant viruses (1.9%) were also detected by the test. This multiplex RT-qPCR assay provides a fast and specific diagnostic tool for identification of different subtypes and lineages of IAV in pigs, contributing to the monitoring of influenza in swine. 650 $aInfluenza A virus 650 $aSwine 650 $aSuíno 653 $aDiagnóstico rápido 653 $aMultiplex RT-qPCR 653 $aRapid diagnosis 653 $aRT-qPCR multiplex 653 $aSubtipagem 653 $aSubtyping 653 $aVírus da gripe A 700 1 $aGAVA, D. 700 1 $aCANTAO, M. E. 700 1 $aFRANCO, A. C. 773 $tJournal of Virological Methods$gv. 269, p. 43-48, 2019.
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