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| Registros recuperados : 148 | |
| 41. |  | SCHAEFER, R.; GAVA, D.; HAACH, V.; SERRÃO, V. H. B.; CANTAO, M. E.; ZANELLA, J. R. C. Genetic diversity of porcine circovirus type 2 causing clinical disease in Brazilian pig herds concomitant with PCV2 vaccination. Virus Reviews & Research, Brasília, DF, v. 20, p. 247-248, Oct. 2015. Supplement 1, ref. VV335. Edição dos Resumos do XXVI Brazilian Congress of Virology, X Mercosur Meeting of Virology, Florianóplis, SC, Oct. 2015.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 43. | | GAVA, D.; SOUZA, C. K.; SCHAEFER, R.; VINCENT, A. L.; CANTAO, M. E.; COLDEBELLA, A.; ZANELLA, J. R. C. A TaqMan-based real-time PCR for detection and quantification of porcine parvovirus 4. Journal of Virological Methods, v. 219, p. 14-17, 2015.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 44. | | GAVA, D.; SERRÃO, V. H. B.; FERNANDES, L. T.; CANTAO, M. E.; ZANELLA, J. R. C.; MORES, N.; SCHAEFER, R. Structure analysis of capsid protein of porcine circovirus type 2 from pigs with systemic disease. Brazilian Journal of Microbiology, v. 49, p. 351-357, 2018.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 46. | | ZANELLA, R.; CANTAO, M. E.; LEDUR, M. C.; MORES, N.; ZANELLA, E. L.; OKINO, C. H.; ZANELLA, J. R. C. Whole genome sequencing on the identification of pathogens involved with high mortality in piglets. In: INTERNATIONAL PIG VETERINARY SOCIETY CONGRESS, 24., Dublin. Abstracts Book... Dublin, Ireland: IPVS, 2016. p. 460.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 48. | | TUPINAMBÁ, D. D.; CANTAO, M. E.; COSTA, O. Y. A.; BERGMANN, J. C.; KRUGER, R. H.; KYAW, C. M.; BARRETO, C. C.; QUIRINO, B. F. Archaeal community changes associated with cultivation of amazon forest soil with oil palm. Archaea, Cairo, v. 2016, article ID 3762159, 14 p., 2016.| Biblioteca(s): Embrapa Agroenergia; Embrapa Suínos e Aves. |
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| 49. | | ROMANO, G. de S.; IBELLI, A. M. G.; PEIXOTO, J. de O.; CANTAO, M. E.; MORES, M. A. Z.; COUTINHO, L. L.; PEDROSA, V. B.; LEDUR, M. C. Análise integrada do transcriptoma e exoma revela novos genes relacionados ao acometimento de hérnia escrotal em suínos. In: SIMPÓSIO BRASILEIRO DE MELHORAMENTO ANIMAL, 13., 2019, Salvador. Anais... Salvador: SBMA, 2019. 1 CD-ROM.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 50. | | CANTAO, M. E.; ZANELLA, R.; PEIXOTO, J. de O.; IBELLI, A. M. G.; PANDOLFI, J. R. C.; SETTLES, M.; LEDUR, M. C. Age effects on gene expression related to the bacterial chondronecrosis with osteomyelitis in broiler. In: INTERNATIONAL PLANT & ANIMAL GENOME, 23., 2015, San Diego, CA. Abstracts? San Diego, 2015.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 51. | | TESSMANN, A. L.; MARCHESI, J. A. P.; ZANELLA, R.; CANTAO, M. E.; PANDOLFI, J. R. C.; IBELLI, A. M. G.; PEIXOTO, J. de O.; LEDUR, M. C. Mining for polymorphisms in the CALB gene and its associations with performance and carcass traits in a paternal broiler line. In: CONGRESSO BRASILEIRO DE GENÉTICA, 59., 2013, Águas de Lindóia. Resumos... Ribeirão Preto: SBG, 2013. p. 149.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 52. | | GOLDONI, I.; IBELLI, A. M. G.; FERNANDES, L. T.; PEIXOTO, J. de O.; HUL, L. M.; CANTAO, M. E.; GOUVEIA, J. J. de S.; LEDUR, M. C. Comprehensive analyses of bone and cartilage transcriptomes evince ion transport, inflammation and cartilage development-related genes involved in chickens femoral head separation. Animals, v. 12, n. 788, 2022.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 53. | | SOUZA, M. R.; IBELLI, A. M. G.; PEIXOTO, J. de O.; MORES, M. A. Z.; CANTAO, M. E.; BOIAGO, M. M.; LEDUR, M. C. Perfil de expressão de genes associados ao colágeno no transcriptoma de suínos normais e afetados com hérnia umbilical. In: SEMINÁRIO DE ENSINO, PESQUISA E EXTENSÃO DA UDESC OESTE, 8.; VIGÉSSIMO OITAVO SEMINÁRIO DE INICIAÇÃO CIENTÍFICA, 28.; PRIMEIRO ENCONTRO DA PÓS-GRADUAÇÃO DA UDESC OESTE, 2018, Chapecó. Anais... Chapecó: UDESC Oeste, 2019. 8º SEPE. 28º SIC. 1 º EPG.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 54. | | SATO, J. P. H.; GAVA, D.; SCHAEFER, R.; SIMON, N. L.; CANTAO, M. E.; ZANELLA, J. R. C.; BARCELLOS, D. E. S. N. de. PCV2 genotype is predominant in captive wild boars Brazil. In: INTERNATIONAL PIG VETERINARY SOCIETY CONGRESS, 23., Cancun. Proceedings... León, México: IPVS, 2014. p. 527| Biblioteca(s): Embrapa Suínos e Aves. |
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| 56. | | CAMPOS, F. G.; IBELLI, A. M. G.; CANTAO, M. E.; OLIVEIRA, H. C. de; PEIXOTO, J. de O.; LEDUR, M. C.; GUIMARÃES, S. E. F. Identification of long non-coding RNAS (LNCRNAS) in swine fetus. In: BRAZILIAN CONGRESS OF GENETICS, 68., 2023, Ouro Preto. Paleogenomics: sequencing ancient DNA. E-book. Ribeirão Preto: Sociedade Brasileira de Genética, 2023. p. 319.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 57. | | JUNQUEIRA, D. M.; TOCHETTO, C.; ANDERSON, T. K.; GAVA, D.; HAACH, V.; CANTAO, M. E.; BAKER, A. L. V.; SCHAEFER, R. Human-to-swine introductions and onward transmission of 2009 H1N1 pandemic influenza viruses in Brazil. Frontiers in Microbiology, v. 14, article 1243567, 2023.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 58. | | JUNQUEIRA, D. M.; ANDERSON, T. K.; TOCHETTO, C.; GAVA, D.; HAACH, V.; CANTAO, M. E.; ZANELLA, J. R. C.; VINCENT, A. L.; SCHAEFER, R. Human-to-swine spillover and onward transmission of H1N1pdm09 in Brazil. In: INTERNATIONAL PIG VETERINARY SOCIETY CONGRESS, 26., 2022, Rio de Janeiro. Proceedings... Rio de Janeiro, RJ: IPVS: Abraves, 2022. p. 690.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 59. | | SILVA, V. H.; PANDOLFI, J. R. C.; GODOY, T. F.; PEIXOTO, J. de O.; TESSMANN, A. L.; CANTAO, M. E.; LEDUR, M. C. Hepatocyte nuclear factor 4 gene associated with bone traits and birth weight in male chickens. In: CONGRESSO BRASILEIRO DE GENÉTICA, 59., 2013, Águas de Lindóia. Resumos... Ribeirão Preto: SBG, 2013. p. 27.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 60. | | BABUJIA, L. C.; SILVA, A. P.; NAKATANI, A. S.; CANTAO, M. E.; VASCONCELOS, A. T. R.; VISENTAINER, J. V.; HUNGRIA, M. Impact of long-term cropping of glyphosate-resistant transgenic soybean [Glycine max (L.) Merr.] on soil microbiome. Transgenic Research, Feb., p.1-6, 2016.| Biblioteca(s): Embrapa Soja; Embrapa Suínos e Aves. |
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| Registros recuperados : 148 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
14/10/2015 |
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Data da última atualização: |
18/02/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
GAVA, D.; SOUZA, C. K.; SCHAEFER, R.; VINCENT, A. L.; CANTAO, M. E.; COLDEBELLA, A.; ZANELLA, J. R. C. |
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Afiliação: |
DANIELLE GAVA, CNPSA; CARINE KUNZLER DE SOUZA, UFRGS; REJANE SCHAEFER, CNPSA; AMY LOUISE VINCENT, USDA/ARS; MAURICIO EGIDIO CANTAO, CNPSA; ARLEI COLDEBELLA, CNPSA; JANICE REIS CIACCI ZANELLA, CNPSA. |
|
Título: |
A TaqMan-based real-time PCR for detection and quantification of porcine parvovirus 4. |
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Ano de publicação: |
2015 |
|
Fonte/Imprenta: |
Journal of Virological Methods, v. 219, p. 14-17, 2015. |
|
DOI: |
http://dx.doi.org/10.1016/j.jviromet.2015.03.011 |
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Idioma: |
Inglês |
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Conteúdo: |
Porcine parvovirus 4 (PPV4) is a DNA virus, and a member of the Parvoviridae family within the Bocavirus genera. It was detected recently in swine, but its epidemiology and pathology remain unclear. A TaqManbased real-time PCR (qPCR) targeting a conserved region of the ORF3 gene of PPV4 was developed. The qPCR detection limit was 9.5 × 101 DNA copies/L. There was no cross-reaction with porcine parvovirus, torque teno virus 1, torque teno virus 2, porcine circovirus type 1, porcine circovirus type 2, or with pseudorabies virus. Two hundred and seventy-two samples, including serum, semen, lungs, feces, ovarian follicular fluids, ovaries and uterus, were evaluated by qPCR and PPV4 was detected in 36 samples (13.2%). When compared with a conventional PCR (cPCR), the qPCR assay was 10 times more sensitive and the detection of PPV4 DNA in field samples was increased 2.5 times. Partial sequencing of PPV4 ORF3 gene, obtained from two pooled samples of uterus and ovaries, revealed a high nucleotide identity (98?99%) with a reference PPV4 sequence. The qPCR can be used as a fast and accurate assay for the detection and quantification of PPV4 in field samples and for epidemiological studies in swine herds. |
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Palavras-Chave: |
Porcine parvovirus; Swine disease. |
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Thesagro: |
Doença animal; Parvovirose; Suíno; Virologia. |
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Thesaurus NAL: |
Parvoviridae; Quantitative polymerase chain reaction; SsDNA viruses; Virology. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02164naa a2200325 a 4500
001 2026395
005 2016-02-18
008 2015 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1016/j.jviromet.2015.03.011$2DOI
100 1 $aGAVA, D.
245 $aA TaqMan-based real-time PCR for detection and quantification of porcine parvovirus 4.$h[electronic resource]
260 $c2015
520 $aPorcine parvovirus 4 (PPV4) is a DNA virus, and a member of the Parvoviridae family within the Bocavirus genera. It was detected recently in swine, but its epidemiology and pathology remain unclear. A TaqManbased real-time PCR (qPCR) targeting a conserved region of the ORF3 gene of PPV4 was developed. The qPCR detection limit was 9.5 × 101 DNA copies/L. There was no cross-reaction with porcine parvovirus, torque teno virus 1, torque teno virus 2, porcine circovirus type 1, porcine circovirus type 2, or with pseudorabies virus. Two hundred and seventy-two samples, including serum, semen, lungs, feces, ovarian follicular fluids, ovaries and uterus, were evaluated by qPCR and PPV4 was detected in 36 samples (13.2%). When compared with a conventional PCR (cPCR), the qPCR assay was 10 times more sensitive and the detection of PPV4 DNA in field samples was increased 2.5 times. Partial sequencing of PPV4 ORF3 gene, obtained from two pooled samples of uterus and ovaries, revealed a high nucleotide identity (98?99%) with a reference PPV4 sequence. The qPCR can be used as a fast and accurate assay for the detection and quantification of PPV4 in field samples and for epidemiological studies in swine herds.
650 $aParvoviridae
650 $aQuantitative polymerase chain reaction
650 $aSsDNA viruses
650 $aVirology
650 $aDoença animal
650 $aParvovirose
650 $aSuíno
650 $aVirologia
653 $aPorcine parvovirus
653 $aSwine disease
700 1 $aSOUZA, C. K.
700 1 $aSCHAEFER, R.
700 1 $aVINCENT, A. L.
700 1 $aCANTAO, M. E.
700 1 $aCOLDEBELLA, A.
700 1 $aZANELLA, J. R. C.
773 $tJournal of Virological Methods$gv. 219, p. 14-17, 2015.
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