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| Registros recuperados : 148 | |
| 21. |  | SCHAEFER, R.; GAVA, D.; HAACH, V.; ZANELLA, J. R. C.; CANTAO, M. E. Novel H1N2 swine influenza virus reassortant strain derived from the pandemic H1N1/2009 virus. In: CONGRESSO BRASILEIRO DE VIROLOGIA, 29.; ENCONTRO DE VIROLOGIA DO MERCOSUL, 13., 2018, Gramado. Resumos... Brasília: SBV, 2018.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 22. | | SCHAEFER, R.; GAVA, D.; HAACH, V.; CANTAO, M. E.; NELSON, M. I. Novel human-like H1N1 swine influenza virus with the six internal genes derived from H1N1/2009 virus. In: CONGRESSO BRASILEIRO DE VIROLOGIA, 28.; ENCONTRO DE VIROLOGIA DO MERCOSUL, 12., 2017, Belo Horizonte. Trabalho pôsteres... Brasília: SBV, 2017.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 25. | | SCHAEFER, R.; CANTAO, M. E.; ROCHA, C. S.; GAVA, D.; ZANELLA, J. R. C. Full genome sequencing of a human-like H1N2 swine influenza virus. In: BRAZILIAN CONGRESS OF VIROLOGY, 23.; MERCOSUR MEETING OF VIROLOGY, 7., 2012, Foz do Iguaçu. Virus and Research? Foz do Iguaçu: SBV, 2012. p. 472-473. Journal of the Brazilian Society for Virology, v. 17, n. 2, suppl. 1, 2012. Projeto/Plano de Ação: 02.11.01.006.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 26. | | SCHAEFER, R.; GAVA, D.; NELSON, M. I.; HAACH, V.; ZANELLA, J. R. C.; CANTAO, M. E. Genetic characterization of influenza viruses circulating within Brazilian swine between 2009 and 2016. In: BRAZILIAN CONGRESS OF VIROLOGY, 27.; MERCOSUR MEETING OF VIROLOGY,11., 2016, Pirenópolis. Abstracts: posters: oral presentation. Brasília, DF: SBV, 2016. Publicado na Virus Reviews and Research, Brasília, n. 20, v. 2, p. 22-23, 2016.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 27. | | SCHAEFER, R.; GAVA, D.; HAACH, V.; CANTAO, M. E.; ZANELLA, J. R. C. Continuous circulation of human-origin seasonal influenza A viruses in swine in Brazil. Virus Reviews & Research, Brasília, DF, v. 20, p. 242, Oct. 2015. Supplement 1, ref. VV301. Edição dos Resumos do XXVI Brazilian Congress of Virology, X Mercosur Meeting of Virology, Florianóplis, SC, Oct. 2015.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 29. | | GOLDONI, I.; IBELLI, A. M. G.; PEIXOTO, J. de O.; HUL, L. M.; CANTAO, M. E.; LEDUR, M. C. Análise comparativa de transcriptoma de frangos normais e afetados com necrose da cabeça do fêmur. In: ENCONTRO DE GENÉTICA DO NORDESTE, 23., 2021, Petrolina. Da produção de alimentos à saúde única: anais. Natal: UFRN: Sociedade Brasileira de Genética Regional Nordeste, 2021. Evento online.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 31. | | GAVA, D.; SCHAEFER, R.; SERRÃO, V. H. B.; CANTAO, M. E.; SILVA, M. C.; ZANELLA, J. R. C. Mutations in the capsid protein of porcine circovirus type 2 indicates a viral immune evasion. In: Journal of the Brazilian Society for Virology, Ribeirão Preto, v. 19, p. 234-235, 2014. Suppl. 2. Edição dos anais do 25º. Brazilian Congress of Virology e 9º. Mercosur Meeting of Virology, Ribeirão Preto, set./out, 2014.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 32. | | TUPINAMBA, D.; CANTAO, M. E.; COSTA, O. Y.; BERGMANN, J. C.; KYAW, C.; BARRETO, C.; QUIRINO, B. F. High-throughput sequencing characterization of land use impact on archaeal community: Amazon native forest and oil palm plantation. In: SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS, 37., 2015, San Diego, California. [Resumos ...] Fairfax: Society for Industrial Microbiology and Biotechnology, 2015. Resumo T67| Biblioteca(s): Embrapa Agroenergia. |
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| 33. | | GAVA, D.; SERRÃO, V. H. B.; FERNANDES, L. T.; CANTAO, M. E.; ZANELLA, J. R. C.; MORES, N.; SCHAEFER, R. Structure analysis of capsid protein of porcine circovirus type 2 from pigs with systemic disease. Brazilian Journal of Microbiology, v. 49, p. 351-357, 2018.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 34. | | GAVA, D.; SOUZA, C. K.; SCHAEFER, R.; VINCENT, A. L.; CANTAO, M. E.; COLDEBELLA, A.; ZANELLA, J. R. C. A TaqMan-based real-time PCR for detection and quantification of porcine parvovirus 4. Journal of Virological Methods, v. 219, p. 14-17, 2015.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 35. | | SOUZA, R. C. de; CANTÃO, M. E.; NOGUEIRA, M. A.; VASCONCELOS, A. T. R.; NICOLAS, M. F.; HUNGRIA, M. Biodiversidade de procariotos em solo sob plantio direto e plantio convencional em rotação ou sucessão de culturas. In: CONGRESSO BRASILEIRO DE MICROBILOGIA, 26.; SIMPÓSIO INTERNACIONAL DE BACTÉRIAS LÁTICAS; ENCONTRO NACIONAL DE PROFESSORES DE MICROBIOLOGIA; SIMPÓSIO DE COLEÇÕES DE CULTURAS, 4., 2011, Foz do Iguaçu. Anais... São Paulo: SBM, 2011. Resumo, 365-1.| Biblioteca(s): Embrapa Soja. |
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| 36. | | ZANELLA, R.; CANTAO, M. E.; PEIXOTO, J. de O.; PANDOLFI, J. R. C.; COUTINHO, L. L.; LEDUR, M. C. Collaborative effort to identify genes involved with bone related traits in broiler chickens. In: INTERNATIONAL PLANT & ANIMAL GENOME, 21., 2013, San Diego. Abstracts... Jersey City: Scherago International, 2013. P0640.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 37. | | TOCHETTO, C.; JUNQUEIRA, D. M.; ANDERSON, T. K.; GAVA, D.; HAACH, V.; CANTAO, M. E.; VINCENT, A. L.; SCHAEFER, R. Introductions of pre-2009 human-origin seasonal influenza A viruses in Brazilian swine herds. In: INTERNATIONAL PIG VETERINARY SOCIETY CONGRESS, 26., 2022, Rio de Janeiro. Proceedings... Rio de Janeiro, RJ: IPVS: Abraves, 2022. p. 700.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 39. | | SCHAEFER, R.; GAVA, D.; CANTAO, M. E.; SERÃO, V. H. B.; SILVA, M. C.; MORES, N.; ZANELLA, J. R. C. PCV2 disease in vaccinated growing pigs in Southern Brazil. In: INTERNATIONAL PIG VETERINARY SOCIETY CONGRESS, 23., Cancun. Proceedings... León, México: IPVS, 2014. p. 522| Biblioteca(s): Embrapa Suínos e Aves. |
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| 40. | | GARCIA, D. A.; LOPES, J. S.; FARAH, M. M.; OTAVIANO, A. R.; CANTAO, M. E.; LEDUR, M. C. Ganhos em acurácia com a utilização de informações genômicas na avaliação genética de suínos. In: SIMPÓSIO BRASILEIRO DE MELHORAMENTO ANIMAL, 11., 2015, Santa Maria. Anais... Santa Maria: SBMA, 2015.| Biblioteca(s): Embrapa Suínos e Aves. |
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| Registros recuperados : 148 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
14/10/2015 |
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Data da última atualização: |
18/02/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
GAVA, D.; SOUZA, C. K.; SCHAEFER, R.; VINCENT, A. L.; CANTAO, M. E.; COLDEBELLA, A.; ZANELLA, J. R. C. |
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Afiliação: |
DANIELLE GAVA, CNPSA; CARINE KUNZLER DE SOUZA, UFRGS; REJANE SCHAEFER, CNPSA; AMY LOUISE VINCENT, USDA/ARS; MAURICIO EGIDIO CANTAO, CNPSA; ARLEI COLDEBELLA, CNPSA; JANICE REIS CIACCI ZANELLA, CNPSA. |
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Título: |
A TaqMan-based real-time PCR for detection and quantification of porcine parvovirus 4. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Journal of Virological Methods, v. 219, p. 14-17, 2015. |
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DOI: |
http://dx.doi.org/10.1016/j.jviromet.2015.03.011 |
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Idioma: |
Inglês |
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Conteúdo: |
Porcine parvovirus 4 (PPV4) is a DNA virus, and a member of the Parvoviridae family within the Bocavirus genera. It was detected recently in swine, but its epidemiology and pathology remain unclear. A TaqManbased real-time PCR (qPCR) targeting a conserved region of the ORF3 gene of PPV4 was developed. The qPCR detection limit was 9.5 × 101 DNA copies/L. There was no cross-reaction with porcine parvovirus, torque teno virus 1, torque teno virus 2, porcine circovirus type 1, porcine circovirus type 2, or with pseudorabies virus. Two hundred and seventy-two samples, including serum, semen, lungs, feces, ovarian follicular fluids, ovaries and uterus, were evaluated by qPCR and PPV4 was detected in 36 samples (13.2%). When compared with a conventional PCR (cPCR), the qPCR assay was 10 times more sensitive and the detection of PPV4 DNA in field samples was increased 2.5 times. Partial sequencing of PPV4 ORF3 gene, obtained from two pooled samples of uterus and ovaries, revealed a high nucleotide identity (98?99%) with a reference PPV4 sequence. The qPCR can be used as a fast and accurate assay for the detection and quantification of PPV4 in field samples and for epidemiological studies in swine herds. |
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Palavras-Chave: |
Porcine parvovirus; Swine disease. |
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Thesagro: |
Doença animal; Parvovirose; Suíno; Virologia. |
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Thesaurus NAL: |
Parvoviridae; Quantitative polymerase chain reaction; SsDNA viruses; Virology. |
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Categoria do assunto: |
-- |
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Marc: |
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001 2026395
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024 7 $ahttp://dx.doi.org/10.1016/j.jviromet.2015.03.011$2DOI
100 1 $aGAVA, D.
245 $aA TaqMan-based real-time PCR for detection and quantification of porcine parvovirus 4.$h[electronic resource]
260 $c2015
520 $aPorcine parvovirus 4 (PPV4) is a DNA virus, and a member of the Parvoviridae family within the Bocavirus genera. It was detected recently in swine, but its epidemiology and pathology remain unclear. A TaqManbased real-time PCR (qPCR) targeting a conserved region of the ORF3 gene of PPV4 was developed. The qPCR detection limit was 9.5 × 101 DNA copies/L. There was no cross-reaction with porcine parvovirus, torque teno virus 1, torque teno virus 2, porcine circovirus type 1, porcine circovirus type 2, or with pseudorabies virus. Two hundred and seventy-two samples, including serum, semen, lungs, feces, ovarian follicular fluids, ovaries and uterus, were evaluated by qPCR and PPV4 was detected in 36 samples (13.2%). When compared with a conventional PCR (cPCR), the qPCR assay was 10 times more sensitive and the detection of PPV4 DNA in field samples was increased 2.5 times. Partial sequencing of PPV4 ORF3 gene, obtained from two pooled samples of uterus and ovaries, revealed a high nucleotide identity (98?99%) with a reference PPV4 sequence. The qPCR can be used as a fast and accurate assay for the detection and quantification of PPV4 in field samples and for epidemiological studies in swine herds.
650 $aParvoviridae
650 $aQuantitative polymerase chain reaction
650 $aSsDNA viruses
650 $aVirology
650 $aDoença animal
650 $aParvovirose
650 $aSuíno
650 $aVirologia
653 $aPorcine parvovirus
653 $aSwine disease
700 1 $aSOUZA, C. K.
700 1 $aSCHAEFER, R.
700 1 $aVINCENT, A. L.
700 1 $aCANTAO, M. E.
700 1 $aCOLDEBELLA, A.
700 1 $aZANELLA, J. R. C.
773 $tJournal of Virological Methods$gv. 219, p. 14-17, 2015.
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