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| Registros recuperados : 33 | |
| 3. |  | VIGNA, B. B. Z.; CANÇADO, L. J.; VALLE, C. B. do; SOUZA, A. P. de. Genetic diversity and molecular mapping of apomixis through microsatellite markers in Brachiaria humidicola (Rendle) Schweick. In:SIMPÓSIO INTERNACIONAL SOBRE MELHORAMENTO DE FORRAGEIRAS, 2., 2009, Campo Grande, MS. [Anais]... Campo Grande, MS: Embrapa Gado de Corte, 2009. II SIMF. Comitê editorial: Liana Jank; Lucimara Chiari; Rosângela Maria Simeão Resende. 4 p. 1 CD-ROM. Resumo B 08.| Biblioteca(s): Embrapa Gado de Corte. |
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| 4. | | ARRUDA, A.; CANCADO, L. J.; CHIARI, L.; VALLE, C. B. do. Transferibilidade de marcadores microssatélites de milho, arroz e trigo para Brachiaria spp. In: JORNADA CIENTÍFICA DA EMBRAPA GADO DE CORTE, 1., 2005, Campo Grande, MS: Embrapa Gado de Corte, 2005. 1 p.| Biblioteca(s): Embrapa Trigo. |
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| 5. | | ARRUDA, A.; CANCADO, L. J.; CHIARI, L.; VALLE, C. B. do. Transferibilidade de marcadores microssatélites de milho, arroz e trigo para Brachiaria spp. In: JORNADA CIENTÍFICA DA EMBRAPA GADO DE CORTE, 1., 2005, Campo Grande, MS: Embrapa Gado de Corte, 2005. 1 p.| Biblioteca(s): Embrapa Gado de Corte. |
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| 7. | | OLIVEIRA, P. B. de; VALLE, C. B. do; SOUZA, A. P.; CANCADO, L. J. Desenvolvimento e caracterização de marcadores moleculares do tipo microssatélites para Brachiaria decumbens In: JORNADA CIENTÍFICA EMBRAPA GADO DE CORTE, 8., 2012, Campo Grande, MS. [Anais da..]. Campo Grande, MS: Embrapa Gado de Corte, 2012. 2 p. (Embrapa Gado de Corte. Documentos, 198).| Biblioteca(s): Embrapa Gado de Corte. |
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| 8. | | VALLE, C. B. do; JANK, L.; RESENDE, R. M. S.; CANÇADO, L. J. O papel da biotecnologia de forrageiras para a produção animal. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 41., 2004, Campo Grande, MS. A produção animal e a segurança alimentar: anais dos simpósios. Campo Grande, MS: Sociedade Brasileira de Zootecnia: Embrapa Gado de Corte, 2004. p. 155-164. CNPGC.| Biblioteca(s): Embrapa Gado de Corte. |
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| 10. | | CANCADO, L. J.; PANIAGO, B. da C.; SANTOS, B. F. dos; GALEANO, S. L. de S.; VALLE, C. B. do. Identification of Microsatellite Markers Useful for Gene Flow Measurement in Brachiaria. In: INTERNATIONAL SYMPOSIUM ON FORAGE BREEDING, 3., 2011, Bonito, MS. Breeding forage for climate change adaptation and mitigation - eco-efficient animal produtction: proceedings. [Campo Grande, MS: Embrapa Gado de Corte], 2011. 145-148 1 CD-ROM| Biblioteca(s): Embrapa Gado de Corte. |
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| 11. | | RESENDE, R. M. S.; RESENDE, M. D. V. de; JANK, L.; VALLE, C. B. do; CANÇADO, L. J.; CHIARI, L. Melhoramento genético de leguminosas forrageiras. In: RESENDE, R. M. S.; VALLE, C. B. do; JANK, L. (Ed.). Melhoramento de forrageiras tropicais. Campo Grande, MS: Embrapa Gado de Corte, 2008. p. 117-159| Biblioteca(s): Embrapa Florestas; Embrapa Gado de Corte. |
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| 14. | | PANIAGO, B. C.; SANTOS, B. F.; MATEUS, R. 2; GALEANO, S. L. S.; VALLE, C. B. do; CANCADO, L. J. Determinação do fluxo gênico em Brachiaria sp. usando marcadores microssatélites. In: JORNADA CIENTÍFICA EMBRAPA GADO DE CORTE, 7., 2011, CAMPO GRANDE, MS. [Anais da]... Campo Grande, MS: Embrapa Gado de Corte, 2011.| Biblioteca(s): Embrapa Gado de Corte. |
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| 15. | | PANIAGO, B. C.; SANTOS, B. F.; MATEUS, R.; WOSNIAK, H.; VALLE, C. B. do; CANCADO, L. J. Determinação do fluxo gênico em Brachiaria sp. usando marcadores microssatélites. In: JORNADA CIENTÍFICA EMBRAPA GADO DE CORTE, 8., 2012, Campo Grande, MS. [Anais da..]. Campo Grande, MS: Embrapa Gado de Corte, 2012. 2 p. (Embrapa Gado de Corte. Documentos, 198).| Biblioteca(s): Embrapa Gado de Corte. |
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| 16. | | ROBLES, C. S.; RESENDE, R. M. S.; CANCADO, L. J.; LEGUIZAMON, G. O. de C.; DOURADO, D.; CHIARI, L. Estudo da variabilidade genética em Stylosanthes guianensis usando marcadores microssatélites. In: SILVEIRA, A. B. da.; DOURADO, D. M.; ANDRADE, L. P. de.; GERVÁSIO, M. S. P.; RIOS, R. A. G. M.; MATIAS, R. (Org.). Bio(in)formação: produção do curso de Ciências Biológicas 2011. Campo Grande, MS: Ed. Anhanguera Uniderp, 2011. p.91-106 1 CD-ROM. Trabalho 5.| Biblioteca(s): Embrapa Gado de Corte. |
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| 17. | | VALERIO, J. R.; CHIARI, L.; RESENDE, R. M. S.; CANCADO, L. J.; SALGADO, L. R.; LEGUIZAMON, G. O. de C. Extração de DNA genômico de Stylosanthes guianensis para análises moleculares. In: JORNADA CIENTÍFICA DA EMBRAPA GADO DE CORTE, 1., 2005, Campo Grande, MS: Embrapa Gado de Corte, 2005. 1 p.| Biblioteca(s): Embrapa Gado de Corte. |
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| 18. | | SOUSA, A. C. B.; CAMPOS, T.; CARVALHO, M. A.; CANCADO, L. J.; JANK, L.; SOUZA, A. P. P-68 microsatellite markers: cross amplification and genetic studies in Centrosema species. In: INTERNATIONAL SYMPOSIUM ON THE MOLECULAR BREEDING OF FORAGE AND TURF: MBFT, 2010, 6., 2010, Buenos Aires. (Resumos...) Buenos Aires: Ediciones INTA, 2010. p. 123-124| Biblioteca(s): Embrapa Cerrados. |
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| 19. | | VIGNA, B. B. Z.; SANTOS, J. C.; PASTINA, M. M.; GAZAFFI, R.; MOLLINARI, M.; CANCADO, L. J.; VALLE, C. B. do; ANTONIO AUGUSTO FRANCO GARCIA; SOUZA, A. P. The Preliminary Genetic Linkage Map of hexaploid Brachiaria humidicola Based on Microsatellite Markers. In: INTERNATIONAL SYMPOSIUM ON FORAGE BREEDING, 3., 2011, Bonito, MS. Breeding forage for climate change adaptation and mitigation - eco-efficient animal produtction: proceedings. [Campo Grande, MS: Embrapa Gado de Corte], 2011. 42-45 1 CD-ROM| Biblioteca(s): Embrapa Gado de Corte. |
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| 20. | | JANK, L.; RESENDE, R. M. S.; VALLE, C. B. do; RESENDE, M. D. V. de; CHIARI, L.; CANÇADO, L. J.; SIMIONI, C. Melhoramento genético de Panicum maximum. In: RESENDE, R. M. S.; VALLE, C. B. do; JANK, L. (Ed.). Melhoramento de forrageiras tropicais. Campo Grande, MS: Embrapa Gado de Corte, 2008. p. 55-87| Biblioteca(s): Embrapa Florestas; Embrapa Gado de Corte. |
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| Registros recuperados : 33 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
30/01/2017 |
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Data da última atualização: |
02/03/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
FERREIRA, R. C. U.; CANÇADO, L. J.; VALLE, C. B. do; CHIARI, L.; SOUZA, A. P. de. |
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Afiliação: |
REBECCA, C. U. FERREIRA, UNICAMP; LETÍCIA J. CANÇADO; CACILDA BORGES DO VALLE, CNPGC; LUCIMARA CHIARI, CNPGC; ANETE P DE SOUZA, UNICAMP. |
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Título: |
Microsatellite loci for Urochloa decumbens (Stapf) R. D. Webster and cross-amplification in other Urochloa species. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
BMC Research Notes, v. 9, n. 152, p. 1-11, 2016. |
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Idioma: |
Português |
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Conteúdo: |
Background: Forage grasses of the African genus Urochloa (syn. Brachiaria) are the basis of Brazilian beef production, and there is a strong demand for high quality, productive and adapted forage plants. Among the approximately 100 species of the genus Urochloa, Urochloa decumbens is one of the most important tropical forage grasses used for pastures due to several of its agronomic attributes. However, the level of understanding of these attributes and the tools with which to control them at the genetic level are limited, mainly due to the apomixis and ploidy level of this species. In this context, the present study aimed to identify and characterize molecular microsatellite markers of U. decumbens and to evaluate their cross-amplification in other Urochloa species. Findings: Microsatellite loci were isolated from a previously constructed enriched library from one U. decumbens genotype. Specific primers were designed for one hundred thirteen loci, and ninety-three primer pairs successfully amplified microsatellite regions, yielding an average of 4.93 alleles per locus. The polymorphism information content ( IC) values of these loci ranged from 0.26 to 0.85 (average 0.68), and the associated discriminating power (DP) values ranged from 0.22 to 0.97 (average 0.77). Cross-amplification studies demonstrated the potential transferability of these microsatellites to four other Urochloa species. Structure analysis revealed the existence of three distinct groups, providing evidence in the allelic pool that U. decumbens is closely related to Urochloa ruziziensis and Urochloa brizantha. The genetic distance values determined using Jaccard?s coefficient ranged from 0.06 to 0.76. Conclusions: The microsatellite markers identified in this study are the first set of molecular markers for U. decumbens species. Their availability will facilitate understanding the genetics of this and other Urochloa species and breeding them, and will be useful for germplasm characterization, linkage mapping and marker-assisted selection. Keywords: Enriched library, Forage, Signalgrass, Simple sequence repeat, Transferability MenosBackground: Forage grasses of the African genus Urochloa (syn. Brachiaria) are the basis of Brazilian beef production, and there is a strong demand for high quality, productive and adapted forage plants. Among the approximately 100 species of the genus Urochloa, Urochloa decumbens is one of the most important tropical forage grasses used for pastures due to several of its agronomic attributes. However, the level of understanding of these attributes and the tools with which to control them at the genetic level are limited, mainly due to the apomixis and ploidy level of this species. In this context, the present study aimed to identify and characterize molecular microsatellite markers of U. decumbens and to evaluate their cross-amplification in other Urochloa species. Findings: Microsatellite loci were isolated from a previously constructed enriched library from one U. decumbens genotype. Specific primers were designed for one hundred thirteen loci, and ninety-three primer pairs successfully amplified microsatellite regions, yielding an average of 4.93 alleles per locus. The polymorphism information content ( IC) values of these loci ranged from 0.26 to 0.85 (average 0.68), and the associated discriminating power (DP) values ranged from 0.22 to 0.97 (average 0.77). Cross-amplification studies demonstrated the potential transferability of these microsatellites to four other Urochloa species. Structure analysis revealed the existence of three distinct groups, providing evidence ... Mostrar Tudo |
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Palavras-Chave: |
Enriched library; Transferability. |
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Thesaurus NAL: |
Forage; Microsatellite repeats; Urochloa brizantha. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/154227/1/Microsatellite-loci-for-Urochloa.pdf
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Marc: |
LEADER 02829naa a2200229 a 4500
001 2062128
005 2017-03-02
008 2016 bl uuuu u00u1 u #d
100 1 $aFERREIRA, R. C. U.
245 $aMicrosatellite loci for Urochloa decumbens (Stapf) R. D. Webster and cross-amplification in other Urochloa species.$h[electronic resource]
260 $c2016
520 $aBackground: Forage grasses of the African genus Urochloa (syn. Brachiaria) are the basis of Brazilian beef production, and there is a strong demand for high quality, productive and adapted forage plants. Among the approximately 100 species of the genus Urochloa, Urochloa decumbens is one of the most important tropical forage grasses used for pastures due to several of its agronomic attributes. However, the level of understanding of these attributes and the tools with which to control them at the genetic level are limited, mainly due to the apomixis and ploidy level of this species. In this context, the present study aimed to identify and characterize molecular microsatellite markers of U. decumbens and to evaluate their cross-amplification in other Urochloa species. Findings: Microsatellite loci were isolated from a previously constructed enriched library from one U. decumbens genotype. Specific primers were designed for one hundred thirteen loci, and ninety-three primer pairs successfully amplified microsatellite regions, yielding an average of 4.93 alleles per locus. The polymorphism information content ( IC) values of these loci ranged from 0.26 to 0.85 (average 0.68), and the associated discriminating power (DP) values ranged from 0.22 to 0.97 (average 0.77). Cross-amplification studies demonstrated the potential transferability of these microsatellites to four other Urochloa species. Structure analysis revealed the existence of three distinct groups, providing evidence in the allelic pool that U. decumbens is closely related to Urochloa ruziziensis and Urochloa brizantha. The genetic distance values determined using Jaccard?s coefficient ranged from 0.06 to 0.76. Conclusions: The microsatellite markers identified in this study are the first set of molecular markers for U. decumbens species. Their availability will facilitate understanding the genetics of this and other Urochloa species and breeding them, and will be useful for germplasm characterization, linkage mapping and marker-assisted selection. Keywords: Enriched library, Forage, Signalgrass, Simple sequence repeat, Transferability
650 $aForage
650 $aMicrosatellite repeats
650 $aUrochloa brizantha
653 $aEnriched library
653 $aTransferability
700 1 $aCANÇADO, L. J.
700 1 $aVALLE, C. B. do
700 1 $aCHIARI, L.
700 1 $aSOUZA, A. P. de
773 $tBMC Research Notes$gv. 9, n. 152, p. 1-11, 2016.
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