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| 1. |  | CONCEIÇÃO, M. A. F.; ZEOLI, J. de J. S.; PAULA, M. V. B de. Irrigation cutoff on 'BRS Clara' seedless grapevines during berry ripening stage. Ciência e Técnica Vitivinícola, Porto, v. 28, t. 2, p. 167-170, 2013. Número especial. Resumo expandido apresentado no 18º Simpósio Internacional GiESCO, 7 a 11 de julho de 2013, Porto.| Biblioteca(s): Embrapa Uva e Vinho. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
11/12/2013 |
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Data da última atualização: |
18/03/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
SANTOS, J. M. L. dos; MONTEIRO, J. P.; RIBEIRO, W. L. C.; MACEDO, I. T. F.; CAMURÇA-VASCONCELOS, A. L. F.; VIEIRA, L. da S.; BEVILÁQUA, C. M. L. |
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Afiliação: |
Jessica Maria Leite dos Santos, Pós-graduação - Universidade Estadual do Ceará (UECe) - Fortaleza, CE, Brazil; JOMAR PATRICIO MONTEIRO, CNPC; Wesley Lyeverton Correia Ribeiro, Pós-graduação - UECe - Fortaleza, CE, Brazil; Iara Tersia Freitas Macedo, UECe - Fortaleza, CE, Brazil; Ana Lourdes Fernandes Camurça-Vasconcelos, Pós-graduação - UECe - Fortaleza, CE, Brazil; LUIZ DA SILVA VIEIRA, CNPC; Claudia Maria Leal Bevilaqua, Pós-graduação - UECe - Fortaleza, CE, Brazil. |
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Título: |
Identification and quantification of benzimidazole resistance polymorphisms in Haemonchus contortus isolated in Northeastern Brazil. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Veterinary Parasitology, v. 199, n. 3/4, p. 160-164, Jan. 2014. |
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DOI: |
http://dx.doi.org/10.1016/j.vetpar.2013.11.006 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Haemonchus contortus is the most prevalent nematode in Brazil. The objective of this study was to select 6 populations of H. contortus of known or suspected benzimidazole resistance status and characterize these using quantitative real-time polymerase chain reaction (qPCR) for single nucleotide polymorphisms (SNPs) F200Y, F167Y and E198A in the ?-tubulin isotype 1 gene. qPCR was performed using DNA from a pool of 10 adult male H. contortus from a single animal per farm. Faecal egg count reduction test (FECRT) and egg hatch test (EHT) were used to determine the resistance status. Samples were obtained from 6 farms located in 5 counties in the Ceará State: Tauá, Boa Viagem, Quixadá, Santa Quitéria and Solonópole. The inbred-susceptible-Edinburgh (ISE) isolate was used as reference for comparative purposes in the qPCR. Benzimidazole resistance was detected by FECRT on all farms with efficacy values ranging from 0 to 51%. EC50 values as determined by EHT were all above 1.49 ug/ml. High frequencies of the resistant SNPs F200Y and F167Y alleles were detected but no resistance was detected at SNP E198A. Our results suggest that the SNPs F167Y and F200Y are both important for benzimidazole resistance in the studied populations. |
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Palavras-Chave: |
Gastrointestinal diseases; Helmintose; Nematóide gastrintestinal; PCR; QPCR; Quantitative real-time PCR; Resistance to anthelmintics; Resistência anti-helmíntica. |
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Thesagro: |
Anti-helmíntico; Haemonchus contortus; Nematóide; Ovino. |
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Thesaurus NAL: |
benzimidazole; Sheep. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02444naa a2200373 a 4500
001 1973510
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008 2014 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1016/j.vetpar.2013.11.006$2DOI
100 1 $aSANTOS, J. M. L. dos
245 $aIdentification and quantification of benzimidazole resistance polymorphisms in Haemonchus contortus isolated in Northeastern Brazil.$h[electronic resource]
260 $c2014
520 $aAbstract: Haemonchus contortus is the most prevalent nematode in Brazil. The objective of this study was to select 6 populations of H. contortus of known or suspected benzimidazole resistance status and characterize these using quantitative real-time polymerase chain reaction (qPCR) for single nucleotide polymorphisms (SNPs) F200Y, F167Y and E198A in the ?-tubulin isotype 1 gene. qPCR was performed using DNA from a pool of 10 adult male H. contortus from a single animal per farm. Faecal egg count reduction test (FECRT) and egg hatch test (EHT) were used to determine the resistance status. Samples were obtained from 6 farms located in 5 counties in the Ceará State: Tauá, Boa Viagem, Quixadá, Santa Quitéria and Solonópole. The inbred-susceptible-Edinburgh (ISE) isolate was used as reference for comparative purposes in the qPCR. Benzimidazole resistance was detected by FECRT on all farms with efficacy values ranging from 0 to 51%. EC50 values as determined by EHT were all above 1.49 ug/ml. High frequencies of the resistant SNPs F200Y and F167Y alleles were detected but no resistance was detected at SNP E198A. Our results suggest that the SNPs F167Y and F200Y are both important for benzimidazole resistance in the studied populations.
650 $abenzimidazole
650 $aSheep
650 $aAnti-helmíntico
650 $aHaemonchus contortus
650 $aNematóide
650 $aOvino
653 $aGastrointestinal diseases
653 $aHelmintose
653 $aNematóide gastrintestinal
653 $aPCR
653 $aQPCR
653 $aQuantitative real-time PCR
653 $aResistance to anthelmintics
653 $aResistência anti-helmíntica
700 1 $aMONTEIRO, J. P.
700 1 $aRIBEIRO, W. L. C.
700 1 $aMACEDO, I. T. F.
700 1 $aCAMURÇA-VASCONCELOS, A. L. F.
700 1 $aVIEIRA, L. da S.
700 1 $aBEVILÁQUA, C. M. L.
773 $tVeterinary Parasitology$gv. 199, n. 3/4, p. 160-164, Jan. 2014.
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Embrapa Caprinos e Ovinos (CNPC) |
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