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| 18. |  | MUCHAILH, M. C.; RODERJAN, C. V.; CAMPOS, J. B.; MACHADO, A. L. T.; CURCIO, G. R. Metodologia de planejamento de paisagens fragmentadas visando a formação de corredores ecológicos. Floresta, Curitiba, v. 40, n. 1, p. 147-162, jan./mar. 2010.| Biblioteca(s): Embrapa Florestas. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Corte. Para informações adicionais entre em contato com cnpgc.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
21/03/2019 |
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Data da última atualização: |
21/03/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, A. L. B. de; MACEDO, G. C. de; ROSINHA, G. M. S.; MELGAREJO, J. L.; ALVES, A. G. L.; BARRETO, W. T. G.; SANTOS, F. M.; CAMPOS, J. B. V.; HERRERA, H. M.; OLIVEIRA, C. E. de. |
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Afiliação: |
Ana Laura Bello de Oliveira, Universidade Católica Dom Bosco - UCDB; Gabriel Carvalho de Macedo, Universidade Católica Dom Bosco - UCDB; GRACIA MARIA SOARES ROSINHA, CNPGC; Jhessyca Leal Melgarejo, Universidade Católica Dom Bosco - UCDB; Andreza Gabriela Leão Alves, Universidade Católica Dom Bosco - UCDB; Wanessa Teixeira Gomes Barreto, Universidade Católica Dom Bosco - UCDB; Filipe Martins Santos, Universidade Católica Dom Bosco - UCDB; João Bosco Vilela Campos, Universidade Católica Dom Bosco - UCDB; Heitor Miraglia Herrera, Universidade Católica Dom Bosco - UCDB; Carina Elisei de Oliveira, Universidade Católica Dom Bosco - UCDB. |
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Título: |
Detection of Brucella spp. in dogs at Pantanal wetlands. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Brazilian Journal of Microbiology, v. 50, n. 1, p. 307-312, 2019. |
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Idioma: |
Inglês |
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Conteúdo: |
Canine brucellosis is an infectious disease that produces reproductive disease in both males and females. Although Brucella canis is more common, the infection by Brucella abortus is more frequent in dogs sharing habitats with livestock and wild animals.We decided to investigate the role of dogs in the maintenance of Brucella spp. in the Pantanal wetland. Serum and whole blood samples were collected from 167 dogs. To detect antibodies against B. abortus and B. canis, buffered acidified plate antigen (BAPA) and agar gel immunodiffusion (AGID) tests were performed. To detect Brucella spp., B. abortus and B. canis DNA, PCR was performed using the bcsp31, BruAb2_0168, and BR00953 genes, respectively. To confirm the PCR results, three bcsp31 PCR products were sequenced and compared with sequences deposited in GenBank. The seropositivity rates of 7.8% and 9% were observed for the AGID and BAPA tests, respectively. Positivity rates of 45.5% and 10.8% were observed when testing bcsp31 and BruAb2_0168, respectively, while there was no positivity for BR00953. The sequenced products had 110 base pairs that aligned with 100% identity to B. abortus, B. canis, and B. suis. Considering our results, dogs may be acting as maintenance hosts of Brucella spp. in the Pantanal region. |
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Palavras-Chave: |
Molecular tests; Reservoir host. |
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Thesaurus NAL: |
Pantanal; Serology. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02067naa a2200277 a 4500
001 2107381
005 2019-03-21
008 2019 bl uuuu u00u1 u #d
100 1 $aOLIVEIRA, A. L. B. de
245 $aDetection of Brucella spp. in dogs at Pantanal wetlands.$h[electronic resource]
260 $c2019
520 $aCanine brucellosis is an infectious disease that produces reproductive disease in both males and females. Although Brucella canis is more common, the infection by Brucella abortus is more frequent in dogs sharing habitats with livestock and wild animals.We decided to investigate the role of dogs in the maintenance of Brucella spp. in the Pantanal wetland. Serum and whole blood samples were collected from 167 dogs. To detect antibodies against B. abortus and B. canis, buffered acidified plate antigen (BAPA) and agar gel immunodiffusion (AGID) tests were performed. To detect Brucella spp., B. abortus and B. canis DNA, PCR was performed using the bcsp31, BruAb2_0168, and BR00953 genes, respectively. To confirm the PCR results, three bcsp31 PCR products were sequenced and compared with sequences deposited in GenBank. The seropositivity rates of 7.8% and 9% were observed for the AGID and BAPA tests, respectively. Positivity rates of 45.5% and 10.8% were observed when testing bcsp31 and BruAb2_0168, respectively, while there was no positivity for BR00953. The sequenced products had 110 base pairs that aligned with 100% identity to B. abortus, B. canis, and B. suis. Considering our results, dogs may be acting as maintenance hosts of Brucella spp. in the Pantanal region.
650 $aPantanal
650 $aSerology
653 $aMolecular tests
653 $aReservoir host
700 1 $aMACEDO, G. C. de
700 1 $aROSINHA, G. M. S.
700 1 $aMELGAREJO, J. L.
700 1 $aALVES, A. G. L.
700 1 $aBARRETO, W. T. G.
700 1 $aSANTOS, F. M.
700 1 $aCAMPOS, J. B. V.
700 1 $aHERRERA, H. M.
700 1 $aOLIVEIRA, C. E. de
773 $tBrazilian Journal of Microbiology$gv. 50, n. 1, p. 307-312, 2019.
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