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Registros recuperados : 19 | |
6. | | AZOUBEL, P. M.; SOUSA, R. M. de; CAMPOS, A. B.; CABRAL, A. C. P. L. F. Desidratação osmótica de melão 'Pele de Sapo': influencia das variáveis de processo. In: SIMPÓSIO BRASILEIRO DE PÓS-COLHEITA DE FRUTAS, HORTALIÇAS E FLORES, 2., 2007, Viçosa, MG. Palestras e resumos... Viçosa, MG: UFV, 2007. p. 340. Biblioteca(s): Embrapa Semiárido. |
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8. | | CABRAL, A. C. P. L. F.; AZOUBEL, P. M.; SILVA, F. O. da; SOUSA, R. M. de; CAMPOS, A. B. Cinética de secagem de manga variedade Tommy Atkins com e sem pré-tratamento osmótico. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA SEMI-ÁRIDO, 1., 2006, Petrolina. Anais... Petrolina: Embrapa Semi-Árido, 2006. p. 27-31. (Embrapa Semi-Árido. Documentos, 197). Biblioteca(s): Embrapa Semiárido. |
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9. | | UCKER, F. E.; CAMPOS, A. B. de; HERNANI, L. C.; MELO, A. da S.; MACEDO, J. R. de. Fate of potassium in sandy soils from the Cerrado Biome, Brazil. In: CAMPOS, A. B. de; GABRIELS, D.; LUJÁN, D. L. (Ed.). Impacts of agrosystems on the environment. Ghent: Unesco, 2015. cap. 3, p. 111-116. Selected papers presented at the AgroEnviron 2014 Symposium. Biblioteca(s): Embrapa Solos. |
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11. | | UCKER, F. E.; DE-CAMPOS, A. B.; HERNANI, L. C.; MACEDO, J. R. de; MELO, A. da S. Movimentação vertical do íon potássio em Neossolos Quartzarênicos sob cultivo com cana-de-açúcar. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 51, n. 9, p. 1548-1556, set. 2016. Biblioteca(s): Embrapa Solos; Embrapa Unidades Centrais. |
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12. | | SOUSA, R. F. de; NASCIMENTO, J. L. do; FERNANDES, E. P.; LEANDRO, W. M.; CAMPOS, A. B. de. Matéria orgânica e textura do solo em veredas conservadas e antropizadas no bioma Cerrado. Revista Brasileira de Engenharia Agrícola e Ambiental, v. 15, n. 8, p. 861-866, ago., 2011. Biblioteca(s): Embrapa Algodão. |
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13. | | UCKER, F. E.; CAMPOS, A. B. de; PEREZ, D. V.; HERNANI, L. C.; MACEDO, J. R. de; MELO, A. da S. Comportamento de nutrientes em solos frágeis sob cultivo de cana-de-açúcar na região de Mineiros, GO. In: CASTRO, S. S. de; HERNANI, L. C. (Ed.). Solos frágeis: caracterização, manejo e sustentabilidade. Brasília, DF: Embrapa, 2015. pt. 2, cap. 2, p. 185-206. Biblioteca(s): Embrapa Solos. |
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14. | | UCKER, F. E.; CAMPOS, A. B. de; HERNANI, L. C.; MELO, A.; MACEDO, J. R. de; SANTOS, F. C. V. dos. Assessment of K transport in sandy soils under sugarcane in the Cerrado Biome, Brazil. In: INTERNATIONAL SYMPOSIUM AGROENVIRON, 9., 2014, Goiânia. Impacts of agrosystems on the environment: challenges and opportunities: abstract. Goiânia: UFG, 2014. Biblioteca(s): Embrapa Solos. |
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15. | | SILVA, M. A. S. da; KLIEMANN, H. J.; DE-CAMPOS, A. B.; MADARI, B. E.; BORGES, J. D.; GONÇALVES, J. M. Effects of vinasse irrigation on effluent ionic concentration in Brazilian Oxisols. African Journal of Agricultural Research, v. 8, n. 45, p. 5663-5671, Nov. 2013. Biblioteca(s): Embrapa Arroz e Feijão. |
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16. | | PINELLI, L. de L. de O.; ROCHA, T. O.; MORETTI, C. L.; CAMPOS, A. B.; BRASILEIRO, A. V.; GLEICY, G.; SANTANA, M. A.; SANTOS, M. S. dos; CHIARELLO, M. D. Caracterização química de morangos 'Osogrande' e 'Camino Real' em diferentes estágios de maturação. In: CONGRESSO BRASILEIRO DE CIÊNCIA E TECNOLOGIA DOS ALIMENTOS, 21.; SEMINÁRIO LATINOAMERICANO E DO CARIBE DE CIÊNCIA E TECNOLOGIA DE ALIMENTOS, 15., 2008, Belo Horizonte. Anais... Belo Horizonte: Sociedade Brasileira de Ciência e Tecnologia de Alimentos, 2008. 3 p. Biblioteca(s): Embrapa Hortaliças. |
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17. | | PINELLI, L. de L. de O.; ROCHA, T. O.; MORETTI, C. L.; CAMPOS, A. B.; BRASILEIRO, A. V.; GLEICY, G.; SANTANA, M. A.; SANTOS, M. S. dos; CHIARELLO, M. D. Antioxidantes em morangos 'Osogrande' e 'Camino Real' armazenados a 5 e a 15ºC. In: CONGRESSO BRASILEIRO DE CIÊNCIA E TECNOLOGIA DOS ALIMENTOS, 21.; SEMINÁRIO LATINOAMERICANO E DO CARIBE DE CIÊNCIA E TECNOLOGIA DE ALIMENTOS, 15., 2008, Belo Horizonte. Anais... Belo Horizonte: Sociedade Brasileira de Ciência e Tecnologia de Alimentos, 2008. 3 p. Biblioteca(s): Embrapa Hortaliças. |
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18. | | PINELI, L. de L. de O.; ROCHA, T. O.; MORETTI, C. L.; CAMPOS, A. B.; BRASILEIRO, A. V.; GLEICY, G.; SANTANA, M. A.; SANTOS, M. S. dos; CHIARELLO, M. D. Variáveis químicas de qualidade de morangos 'Osogrande' e 'Camino Real' produzidos no Distrito Federal armazenados a 5 e 15ºC. In: CONGRESSO BRASILEIRO DE CIÊNCIA E TECNOLOGIA DOS ALIMENTOS, 21.; SEMINÁRIO LATINOAMERICANO E DO CARIBE DE CIÊNCIA E TECNOLOGIA DE ALIMENTOS, 15., 2008, Belo Horizonte. Anais... Belo Horizonte: Sociedade Brasileira de Ciência e Tecnologia de Alimentos, 2008. 3 p. Biblioteca(s): Embrapa Hortaliças. |
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19. | | ABREU, J. T.; MOURÃO, M. M.; SANTOS, C. E.; VELOSO, C. J. M.; RESENDE, J. S.; FLATSCHART, R. B.; FOLGUERAS-FLATSCHAR, A. V.; JUNIOR, S. N.; SANTORO, M. M.; MENDES, A. C. R.; FRANCO, G. R.; SILVA, A.; CAMPOS, A. B.; FERNANDEZ, S. Molecular studies of the brazilian infectious bronchitis virus isolates. Revista Brasileira de Ciência Avícola, Campinas, v. 12, n. 2, p. 107-110, 2010. Biblioteca(s): Embrapa Milho e Sorgo. |
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Registros recuperados : 19 | |
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Registro Completo
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
28/10/2010 |
Data da última atualização: |
28/05/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 2 |
Autoria: |
ABREU, J. T.; MOURÃO, M. M.; SANTOS, C. E.; VELOSO, C. J. M.; RESENDE, J. S.; FLATSCHART, R. B.; FOLGUERAS-FLATSCHAR, A. V.; JUNIOR, S. N.; SANTORO, M. M.; MENDES, A. C. R.; FRANCO, G. R.; SILVA, A.; CAMPOS, A. B.; FERNANDEZ, S. |
Afiliação: |
AUREA VALADARES FOLGUERAS FLATSCHAR, CNPMS. |
Título: |
Molecular studies of the brazilian infectious bronchitis virus isolates. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
Revista Brasileira de Ciência Avícola, Campinas, v. 12, n. 2, p. 107-110, 2010. |
DOI: |
10.1590/S1516-635X2010000200005 |
Idioma: |
Inglês |
Conteúdo: |
Avian infectious bronchitis virus (IBV) isolates have been widely characterized by reverse transcription followed by polymerase chain reaction and DNA sequencing. In present study, these techniques were applied to three viral genomic regions comprising the complete and/or a partial S1 segment, S2 and nucleocapsid genes. DNA sequences from viral isolates obtained from 1972 to 1989 and from 2006 to 2008 were compared. High similarity (>90%) was observed among some of the genomic segments, including S1 hypervariable region, which could suggest a common origin or ancestry. DNA sequences from S2 and N protein genes obtained from different infected tissues of the same flock were analyzed, and a clear segregation between respiratory and intestinal tract was observed. Therefore, these data suggest cocirculation of more than one viral strain in the same flock. 57.1% of DNA sequences from the S1 complete segment samples, 53.3% from the S2 fragment and 62.5% from the partial N gene were found to be different from analyzed sequences from reference strains leading to the conclusion that parte of viral isolates included in this study may be considered region specific. Considering the simultaneous analysis of the three genes, a large IBV genetic profile was observed in both old and recent isolates groups. However, most prominent diversity between viral isolates was obtained in the period from 1972 and 1989, showing the presence of a large number of variants in the state of Minas Gerais before the official approval of vaccination (1980). MenosAvian infectious bronchitis virus (IBV) isolates have been widely characterized by reverse transcription followed by polymerase chain reaction and DNA sequencing. In present study, these techniques were applied to three viral genomic regions comprising the complete and/or a partial S1 segment, S2 and nucleocapsid genes. DNA sequences from viral isolates obtained from 1972 to 1989 and from 2006 to 2008 were compared. High similarity (>90%) was observed among some of the genomic segments, including S1 hypervariable region, which could suggest a common origin or ancestry. DNA sequences from S2 and N protein genes obtained from different infected tissues of the same flock were analyzed, and a clear segregation between respiratory and intestinal tract was observed. Therefore, these data suggest cocirculation of more than one viral strain in the same flock. 57.1% of DNA sequences from the S1 complete segment samples, 53.3% from the S2 fragment and 62.5% from the partial N gene were found to be different from analyzed sequences from reference strains leading to the conclusion that parte of viral isolates included in this study may be considered region specific. Considering the simultaneous analysis of the three genes, a large IBV genetic profile was observed in both old and recent isolates groups. However, most prominent diversity between viral isolates was obtained in the period from 1972 and 1989, showing the presence of a large number of variants in the state of Minas Gerais bef... Mostrar Tudo |
Thesagro: |
Genética; Virus. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/32305/1/Molecular-studies.pdf
|
Marc: |
LEADER 02461naa a2200313 a 4500 001 1865545 005 2018-05-28 008 2010 bl uuuu u00u1 u #d 024 7 $a10.1590/S1516-635X2010000200005$2DOI 100 1 $aABREU, J. T. 245 $aMolecular studies of the brazilian infectious bronchitis virus isolates.$h[electronic resource] 260 $c2010 520 $aAvian infectious bronchitis virus (IBV) isolates have been widely characterized by reverse transcription followed by polymerase chain reaction and DNA sequencing. In present study, these techniques were applied to three viral genomic regions comprising the complete and/or a partial S1 segment, S2 and nucleocapsid genes. DNA sequences from viral isolates obtained from 1972 to 1989 and from 2006 to 2008 were compared. High similarity (>90%) was observed among some of the genomic segments, including S1 hypervariable region, which could suggest a common origin or ancestry. DNA sequences from S2 and N protein genes obtained from different infected tissues of the same flock were analyzed, and a clear segregation between respiratory and intestinal tract was observed. Therefore, these data suggest cocirculation of more than one viral strain in the same flock. 57.1% of DNA sequences from the S1 complete segment samples, 53.3% from the S2 fragment and 62.5% from the partial N gene were found to be different from analyzed sequences from reference strains leading to the conclusion that parte of viral isolates included in this study may be considered region specific. Considering the simultaneous analysis of the three genes, a large IBV genetic profile was observed in both old and recent isolates groups. However, most prominent diversity between viral isolates was obtained in the period from 1972 and 1989, showing the presence of a large number of variants in the state of Minas Gerais before the official approval of vaccination (1980). 650 $aGenética 650 $aVirus 700 1 $aMOURÃO, M. M. 700 1 $aSANTOS, C. E. 700 1 $aVELOSO, C. J. M. 700 1 $aRESENDE, J. S. 700 1 $aFLATSCHART, R. B. 700 1 $aFOLGUERAS-FLATSCHAR, A. V. 700 1 $aJUNIOR, S. N. 700 1 $aSANTORO, M. M. 700 1 $aMENDES, A. C. R. 700 1 $aFRANCO, G. R. 700 1 $aSILVA, A. 700 1 $aCAMPOS, A. B. 700 1 $aFERNANDEZ, S. 773 $tRevista Brasileira de Ciência Avícola, Campinas$gv. 12, n. 2, p. 107-110, 2010.
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Embrapa Milho e Sorgo (CNPMS) |
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