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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
19/03/2018 |
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Data da última atualização: |
19/03/2018 |
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Autoria: |
NOQUEIRA, R. de M. S.; SILVA, A. B.; SATO, T. P.; SÁ, J. C. de; SANTOS, A. C. G. dos; AMORIM FILHO, E. F.; VALE, T. L. do; GAZÊTA, G. S. |
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Afiliação: |
Rita de Maria Seabra Nogueira, Departamento de Patologia/Universidade Estadual do Maranhão - UEMA; Arannadia Barbosa Silva, Laboratório de Referência Nacional em Vetores das Riquetsioses - LIRN/IOC/Fiocruz; Tayra Pereira Sato, Laboratório de Referência Nacional em Vetores das Riquetsioses - LIRN/IOC/Fiocruz; Joicy Cortez de Sá, Departamento de Patologia/Universidade Estadual do Maranhão - UEMA; Ana Clara Gomes dos Santos, Departamento de Patologia/Universidade Estadual do Maranhão - UEMA; Edvaldo Franco Amorim Filho, Departamento de Patologia/Universidade Estadual do Maranhão - UEMA; Tássia Lopes do Vale, Departamento de Patologia/Universidade Estadual do Maranhão - UEMA; Gilberto Salles Gazêta, Laboratório de Referência Nacional em Vetores das Riquetsioses - LIRN/IOC/Fiocruz. |
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Título: |
Molecular and serological detection of Theileria equi, Babesia caballi and Anaplasma phagocytophilum in horses and ticks in Maranhão, Brazil. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 12, p. 1416-1422, dezembro 2017. |
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Idioma: |
Inglês |
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Notas: |
Título em português: Detecção molecular e serológica de Theileria equi, Babesia caballi e Anaplasma phagocytophilum em equinos e carrapatos no Maranhão. |
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Conteúdo: |
Equine piroplasmosis is a tick-borne disease caused by the intraeytrhocytic protozoans Babesia caballi and Theileria equi. It has been reported as a main equine parasitic disease. In addition, Anaplasma phagocytophilum, the causative agent of granulocytic ehrlichiosis, causes a seasonal disease in horses. Both diseases, can be detrimental to animal health. In this sense, blood samples and ticks were collected from 97 horses raised in the microregion of Baixada Maranhense, Maranhão State, Brazil. Serum samples were subjected to Indirect Fluorescence Antibody Test (IFAT) and blood samples and ticks to Polymerase Chain Reaction (PCR) to evaluate the infection by Theileria equi, Babesia caballi and Anaplasma phagocytophilum. The overall seroprevalence was 38.14%, 18.55% and 11.34% for T. equi, B. caballi and A. phagocytophilum, respectively. The results of PCR from blood samples showed 13.40% and 3.09% positive samples to T. equi and B. caballi, respectively. A total of 170 tick specimens were collected and identified as Dermacentor nitens, Amblyomma cajennense sensu lato and Rhipicephalus (Boophilus) microplus. It was detected 2.35% (4/170) and 0.59% (1/170) positive tick samples by PCR for T. equi and B. caballi, respectively. All samples were negative to A. phagocytophilum. No statically difference (p>0.05) was observed when gender, age, use of ectoparasiticide and tick presence were analyzed. A BLASTn analysis of the sequenced samples indicated 97 to 100% similarity with T. equi 18S rRNA gene sequences in GenBank and 98 to 100% with B. caballi. Genetic analysis classified the obtained sequences as T. equi and B. caballi cluster, respectively. It can be concluded that these pathogens occur and are circulating in the studied area. MenosEquine piroplasmosis is a tick-borne disease caused by the intraeytrhocytic protozoans Babesia caballi and Theileria equi. It has been reported as a main equine parasitic disease. In addition, Anaplasma phagocytophilum, the causative agent of granulocytic ehrlichiosis, causes a seasonal disease in horses. Both diseases, can be detrimental to animal health. In this sense, blood samples and ticks were collected from 97 horses raised in the microregion of Baixada Maranhense, Maranhão State, Brazil. Serum samples were subjected to Indirect Fluorescence Antibody Test (IFAT) and blood samples and ticks to Polymerase Chain Reaction (PCR) to evaluate the infection by Theileria equi, Babesia caballi and Anaplasma phagocytophilum. The overall seroprevalence was 38.14%, 18.55% and 11.34% for T. equi, B. caballi and A. phagocytophilum, respectively. The results of PCR from blood samples showed 13.40% and 3.09% positive samples to T. equi and B. caballi, respectively. A total of 170 tick specimens were collected and identified as Dermacentor nitens, Amblyomma cajennense sensu lato and Rhipicephalus (Boophilus) microplus. It was detected 2.35% (4/170) and 0.59% (1/170) positive tick samples by PCR for T. equi and B. caballi, respectively. All samples were negative to A. phagocytophilum. No statically difference (p>0.05) was observed when gender, age, use of ectoparasiticide and tick presence were analyzed. A BLASTn analysis of the sequenced samples indicated 97 to 100% similarity with T. ... Mostrar Tudo |
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Palavras-Chave: |
Doença transmitida por vetores; Vector-borne disease. |
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Thesagro: |
Carrapato; Cavalo. |
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Thesaurus Nal: |
Anaplasma phagocytophilum; Babesia caballi; Brazil; Horses; Theileria equi; Ticks. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/174112/1/Molecular-and-serological-detection-of-Theileria.pdf
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Marc: |
LEADER 02954naa a2200337 a 4500
001 2089348
005 2018-03-19
008 2017 bl uuuu u00u1 u #d
100 1 $aNOQUEIRA, R. de M. S.
245 $aMolecular and serological detection of Theileria equi, Babesia caballi and Anaplasma phagocytophilum in horses and ticks in Maranhão, Brazil.$h[electronic resource]
260 $c2017
500 $aTítulo em português: Detecção molecular e serológica de Theileria equi, Babesia caballi e Anaplasma phagocytophilum em equinos e carrapatos no Maranhão.
520 $aEquine piroplasmosis is a tick-borne disease caused by the intraeytrhocytic protozoans Babesia caballi and Theileria equi. It has been reported as a main equine parasitic disease. In addition, Anaplasma phagocytophilum, the causative agent of granulocytic ehrlichiosis, causes a seasonal disease in horses. Both diseases, can be detrimental to animal health. In this sense, blood samples and ticks were collected from 97 horses raised in the microregion of Baixada Maranhense, Maranhão State, Brazil. Serum samples were subjected to Indirect Fluorescence Antibody Test (IFAT) and blood samples and ticks to Polymerase Chain Reaction (PCR) to evaluate the infection by Theileria equi, Babesia caballi and Anaplasma phagocytophilum. The overall seroprevalence was 38.14%, 18.55% and 11.34% for T. equi, B. caballi and A. phagocytophilum, respectively. The results of PCR from blood samples showed 13.40% and 3.09% positive samples to T. equi and B. caballi, respectively. A total of 170 tick specimens were collected and identified as Dermacentor nitens, Amblyomma cajennense sensu lato and Rhipicephalus (Boophilus) microplus. It was detected 2.35% (4/170) and 0.59% (1/170) positive tick samples by PCR for T. equi and B. caballi, respectively. All samples were negative to A. phagocytophilum. No statically difference (p>0.05) was observed when gender, age, use of ectoparasiticide and tick presence were analyzed. A BLASTn analysis of the sequenced samples indicated 97 to 100% similarity with T. equi 18S rRNA gene sequences in GenBank and 98 to 100% with B. caballi. Genetic analysis classified the obtained sequences as T. equi and B. caballi cluster, respectively. It can be concluded that these pathogens occur and are circulating in the studied area.
650 $aAnaplasma phagocytophilum
650 $aBabesia caballi
650 $aBrazil
650 $aHorses
650 $aTheileria equi
650 $aTicks
650 $aCarrapato
650 $aCavalo
653 $aDoença transmitida por vetores
653 $aVector-borne disease
700 1 $aSILVA, A. B.
700 1 $aSATO, T. P.
700 1 $aSÁ, J. C. de
700 1 $aSANTOS, A. C. G. dos
700 1 $aAMORIM FILHO, E. F.
700 1 $aVALE, T. L. do
700 1 $aGAZÊTA, G. S.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 37, n. 12, p. 1416-1422, dezembro 2017.
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Embrapa Unidades Centrais (AI-SEDE) |
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| Registros recuperados : 8 | |
| 1. |  | MIRANDA, M. A. C. de; ALMEIDA, L. A. de; KIIHL, R. A. de S.; CAMPELLO, G. J. A.; KLEPKER, D. BRSMA Boa Vista: nova cultivar de soja para os Estados do Maranhao, Piaui e Tocantins. In: REUNIAO DE PESQUISA DE SOJA DA REGIAO CENTRAL DO BRASIL, 21., 1999, Dourados. Resumos... Dourados: Embrapa Agropecuaria Oeste / Londrina: Embrapa Soja, 1999. p.201. (Embrapa Agropecuaria Oeste. Documentos, 7; Embrapa Soja. Documentos, 134).| Biblioteca(s): Embrapa Soja. |
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| 2. | | MIRANDA, M. A. C. de; KIIHL, R. A. de S.; ALMEIDA, L. A. de; CAMPELLO, G. J. A.; KLEPKER, D. Cultivar de soja BRSMA Babacu. In: REUNIAO DE PESQUISA DE SOJA DA REGIAO CENTRAL DO BRASIL, 21., 1999, Dourados. Resumos... Dourados: Embrapa Agropecuaria Oeste / Londrina: Embrapa Soja, 1999. p.202-203. (Embrapa Agropecuaria Oeste. Documentos, 7; Embrapa Soja. Documentos, 134).| Biblioteca(s): Embrapa Soja. |
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| 3. | | MIRANDA, M. A. C. de; KIIHL, R. A. de S.; ALMEIDA, L. A. de; CAMPELLO, G. J. A.; KLEPKER, D. Cultivar de soja BRSMA Jucara. In: REUNIAO DE PESQUISA DE SOJA DA REGIAO CENTRAL DO BRASIL, 21., 1999, Dourados. Resumos... Dourados: Embrapa Agropecuaria Oeste / Londrina: Embrapa Soja, 1999. p.202. (Embrapa Agropecuaria Oeste. Documentos, 7; Embrapa Soja. Documentos, 134).| Biblioteca(s): Embrapa Soja. |
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| 5. | | MIRANDA, M. A. C.; ALMEIDA, L. A.; KIIHL, R. A. S.; MEYER, M. C.; CAMPELLO, G. J. Cultivar de soja MA/BRS-164 (PATI). In: REUNIAO DE PESQUISA DA SOJA DA REGIAO CENTRAL DO BRASIL, 20., 1998, Londrina. Ata e Resumos. Londrina: EMBRAPA-CNPSo, 1998. p.312. (EMBRAPA-CNPSo. Documentos, 121).| Biblioteca(s): Embrapa Soja. |
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| 6. | | MIRANDA, M. A. C.; ALMEIDA, L. A.; KIIHL, R. A. S.; MEYER, M. C.; CAMPELLO, G. J. Cultivar de soja MA/BRS-165 (Serido RCH). In: REUNIAO DE PESQUISA DA SOJA DA REGIAO CENTRAL DO BRASIL, 20., 1998, Londrina. Ata e Resumos. Londrina: EMBRAPA-CNPSo, 1998. p.311-312. (EMBRAPA-CNPSo. Documentos, 121).| Biblioteca(s): Embrapa Soja. |
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| 7. | | MIRANDA, M. A. C. de; KASTER, M.; ALMEIDA, L. A. de; KIIHL, R. A. de S.; CAMPELLO, G. J. A.; KLEPKER, D. BRSMA Tracaja: nova cultivar precoce de soja para os Estados do Maranhao, Piaui e Tocantins. In: REUNIAO DE PESQUISA DE SOJA DA REGIAO CENTRAL DO BRASIL, 21., 1999, Dourados. Resumos... Dourados: Embrapa Agropecuaria Oeste / Londrina: Embrapa Soja, 1999. p.200-201. (Embrapa Agropecuaria Oeste. Documentos, 7; Embrapa Soja. Documentos, 134).| Biblioteca(s): Embrapa Soja. |
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| 8. | | KIIHL, R. A. S.; ALMEIDA, L. A.; BAYS, I. A.; DALL'AGNOL, A.; GILIOLI, J. L.; MENOSSO, O. G.; TOLEDO, J. F. F.; CARRAO-PANIZZI, M.C.; BERTAGNOLLO, P.; CAMPELLO, G. J.; CARNIELLI, A.; SPEHAR, C. R.; ARANTES, C. R.; ARANTES, N. E.; GOMES, E.; MONTEIRO, P. M. F. O.; ROLIM, R. B. Desenvolvimento de cultivares adaptadas as varias regioes ecologicos e aos varios sistemas de producao. In: REUNIAO DE PESQUISA DE SOJA DA REGIAO SUL, 9., 1981, Passo Fundo. Ata... Passo Fundo: EMBRAPA-CNPT, 1981. p.166.| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 8 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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