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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
09/01/2020 |
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Data da última atualização: |
04/02/2020 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
ZANELLA, L.; DEGENHARDT-GOLDBACH, J.; GERHARDT, I. R.; BESPALHOK FILHO, J. C. |
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Afiliação: |
LAUDIANE ZANELLA, UFPR; JULIANA DEGENHARDT GOLDBACH, CNPF; ISABEL RODRIGUES GERHARDT, CNPTIA; JOÃO CARLOS BESPALHOK FILHO, UFPR. |
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Título: |
RNA interference supression of DOF transcription factor from Populus tremula x Populus alba. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Pesquisa Florestal Brasileira, v. 39, e201902043, p. 322, 2019. |
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Idioma: |
Inglês |
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Notas: |
Na publicação: Isabel Gerhardt. Edição especial dos resumos do IUFRO World Congress, 25., 2019, Curitiba. |
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Conteúdo: |
From the total biomass produced by plants, 70% is represented by cell wall, in which carbon is mainly accumulated in the secondary wall. Therefore, the understanding of the mechanisms that regulate cell wall synthesis and its modification through genetic engineering may lead to the increase of lignocellulosic biomass. Among several functions already attributed to the proteins of the Dof transcription factors, its relation with cell wall formation is one of the most studied. However, the mechanisms involved in this process are not fully elucidated. Populus is the most used model plant for studies with forest species, including functional genomics. The objective of this work was to silence one gene of the Dof family by RNAi in plants of Populus tremula x P. alba, and investigate its function, aiming at the future production of Eucalyptus and Pinus plants with higher biomass production. For this, a silencing vector was constructed with specific primers of a Dof family gene from Populus. To obtain the transgenic plants via Agrobacterium tumefaciens, root segments were used as initial explants. Fifteen events were obtained, and in twelve the Dof expression was suppressed at different levels, according to qPCR analyzes. Of these, on five the expression was highly suppressed, indicating that the gene was successfully silenced. In morphological evaluations after 90 days the events did not present phenotypic differences in relation to non transformed plants. Physiological and histochemical analyzes are underway to evaluate lignin and cellulose contents and structural changes in transformed plants. MenosFrom the total biomass produced by plants, 70% is represented by cell wall, in which carbon is mainly accumulated in the secondary wall. Therefore, the understanding of the mechanisms that regulate cell wall synthesis and its modification through genetic engineering may lead to the increase of lignocellulosic biomass. Among several functions already attributed to the proteins of the Dof transcription factors, its relation with cell wall formation is one of the most studied. However, the mechanisms involved in this process are not fully elucidated. Populus is the most used model plant for studies with forest species, including functional genomics. The objective of this work was to silence one gene of the Dof family by RNAi in plants of Populus tremula x P. alba, and investigate its function, aiming at the future production of Eucalyptus and Pinus plants with higher biomass production. For this, a silencing vector was constructed with specific primers of a Dof family gene from Populus. To obtain the transgenic plants via Agrobacterium tumefaciens, root segments were used as initial explants. Fifteen events were obtained, and in twelve the Dof expression was suppressed at different levels, according to qPCR analyzes. Of these, on five the expression was highly suppressed, indicating that the gene was successfully silenced. In morphological evaluations after 90 days the events did not present phenotypic differences in relation to non transformed plants. Physiological and histoch... Mostrar Tudo |
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Thesaurus Nal: |
Populus alba; Populus tremula. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02291nam a2200181 a 4500
001 2118433
005 2020-02-04
008 2019 bl uuuu u01u1 u #d
100 1 $aZANELLA, L.
245 $aRNA interference supression of DOF transcription factor from Populus tremula x Populus alba.$h[electronic resource]
260 $aPesquisa Florestal Brasileira, v. 39, e201902043, p. 322$c2019
500 $aNa publicação: Isabel Gerhardt. Edição especial dos resumos do IUFRO World Congress, 25., 2019, Curitiba.
520 $aFrom the total biomass produced by plants, 70% is represented by cell wall, in which carbon is mainly accumulated in the secondary wall. Therefore, the understanding of the mechanisms that regulate cell wall synthesis and its modification through genetic engineering may lead to the increase of lignocellulosic biomass. Among several functions already attributed to the proteins of the Dof transcription factors, its relation with cell wall formation is one of the most studied. However, the mechanisms involved in this process are not fully elucidated. Populus is the most used model plant for studies with forest species, including functional genomics. The objective of this work was to silence one gene of the Dof family by RNAi in plants of Populus tremula x P. alba, and investigate its function, aiming at the future production of Eucalyptus and Pinus plants with higher biomass production. For this, a silencing vector was constructed with specific primers of a Dof family gene from Populus. To obtain the transgenic plants via Agrobacterium tumefaciens, root segments were used as initial explants. Fifteen events were obtained, and in twelve the Dof expression was suppressed at different levels, according to qPCR analyzes. Of these, on five the expression was highly suppressed, indicating that the gene was successfully silenced. In morphological evaluations after 90 days the events did not present phenotypic differences in relation to non transformed plants. Physiological and histochemical analyzes are underway to evaluate lignin and cellulose contents and structural changes in transformed plants.
650 $aPopulus alba
650 $aPopulus tremula
700 1 $aDEGENHARDT-GOLDBACH, J.
700 1 $aGERHARDT, I. R.
700 1 $aBESPALHOK FILHO, J. C.
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Registro original: |
Embrapa Agricultura Digital (CNPTIA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
29/05/2013 |
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Data da última atualização: |
29/05/2013 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
MORAES, M. A. de; MORAES, M. L.; NEVES, G.; CAMBUIM, J.; SILVA, P.; AGUIAR, A. V. de. |
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Afiliação: |
Marcela Aparecida de Moraes, UNESP; Mario Luiz Moraes, UNESP; Generci Neves, Resineves Company; José Cambuim, UNESP; Paulo Silva, IPEF; ANANDA VIRGINIA DE AGUIAR, CNPF. |
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Título: |
Expected selection gain for resin-yielding in progenies test of Pinus caribaea var. bahamensis. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
In: CONFERENCE ON BREEDING AND GENETIC RESOURCES OF THE SOUTHERN US AND MEXICAN PINES, 2013, Jacksonville, Florida. Breeding for value in a changing world. [S.l.]: IUFRO, 2013. |
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Descrição Física: |
Disponível online. |
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Idioma: |
Inglês |
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Notas: |
Abstract. IUFRO Working Group 2.02.20. |
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Thesagro: |
Pinus Caribaea; Teste de Progênie. |
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Categoria do assunto: |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/83516/1/2013-Ananda-IUFRO-ExpectedPinus.pdf
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Marc: |
LEADER 00782nam a2200205 a 4500
001 1959042
005 2013-05-29
008 2013 bl uuuu u00u1 u #d
100 1 $aMORAES, M. A. de
245 $aExpected selection gain for resin-yielding in progenies test of Pinus caribaea var. bahamensis.$h[electronic resource]
260 $aIn: CONFERENCE ON BREEDING AND GENETIC RESOURCES OF THE SOUTHERN US AND MEXICAN PINES, 2013, Jacksonville, Florida. Breeding for value in a changing world. [S.l.]: IUFRO$c2013
300 $cDisponível online.
500 $aAbstract. IUFRO Working Group 2.02.20.
650 $aPinus Caribaea
650 $aTeste de Progênie
700 1 $aMORAES, M. L.
700 1 $aNEVES, G.
700 1 $aCAMBUIM, J.
700 1 $aSILVA, P.
700 1 $aAGUIAR, A. V. de
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Embrapa Florestas (CNPF) |
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