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| Registros recuperados : 311 | |
| 161. |  | OLIVEIRA, C. S.; BARROS, B. A. F. de; MONTEIRO, C. A. S.; ROSA, P. M. S.; LEAL, G. R.; SERAPIÃO, R. V.; CAMARGO, L. S. de A. Individual assessment of bovine embryo development using a homemade chamber reveals kinetic patterns of success and failure to reach blastocyst stage. Systems Biology in Reproductive Medicine, v. 65, n. 4, p. 301-311, 2019.| Biblioteca(s): Embrapa Gado de Leite. |
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| 162. | | POLISSENI, J.; GRÁZIB, J. G. V. de; GUERRA, M. DE O.; MILEN, L. C.; CAMARGO, L. S. de A.; PETERS, V. M. Impact of the use of gonadotropins on embryonic development - a rat model. JBRA Assisted Reproduction, v. 17, n. 1, p. 32-36, 2013.| Biblioteca(s): Embrapa Gado de Leite. |
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| 163. | | CAMARGO, L. S. de A.; AGUIRRE-LAVIN, T.; ADENOT, P.; ARAUJO, T. D.; MENDES, V. R. A.; LOURO, I. D.; BEAUJEAN, N.; SOUZA, E. D. Heat shock during in vitro maturation induces chromatin modifications in the bovine embryo. Reproduction, v. 158, n. 4, p. 313-322, 2019.| Biblioteca(s): Embrapa Gado de Leite. |
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| 165. | | OLIVEIRA, C. S.; SERAPIÃO, R. V.; CAMARGO, A. J dos R.; FREITAS, C. de; IGUMA, L. T.; CAMARGO, L. S. de A.; VERNEQUE, R. da S. Maternal breed influence in Holstein-Gyr reciprocal F1 embryos. In: ANNUAL MEETING OF THE BRAZILIAN EMBRYO TECNNOLOGY SOCIETY, 14., 2017, Cabo de Santo Agostinho. Proceedings... Cabo de Santo Agostinho: SBTE, 2017. p. 849.| Biblioteca(s): Embrapa Gado de Leite. |
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| 166. | | PEREIRA, M. M.; BRANDÃO, H. de M.; RAPOSO, N. R. B.; QUINTÃO, C. C. R.; TEIXEIRA, E. de M.; CAMARGO, L. S. de A.; MATTOSO, L. H. C. Toxicidade de nanofibras de celulose em cultura de fibroblastos bovinos. In: WORKSHOP DA REDE DE NANOTECNOLOGIA APLICADA AO AGRONEGÓCIO, 6., 2012, Fortaleza. Anais... São Carlos: Embrapa Instrumentação; Fortaleza: Embrapa Agroindústria Tropical, 2012. p. 443-445. Editores: Maria Alice Martins, MOrsyleide de Freitas Rosa, Men de Sá Moreira de Souza Filho, Nicodemos Moreira dos Santos Junior, Odílio Benedito Garrido de Assis, Caue Ribeiro, Luiz Henrique Capparelli Mattoso.| Biblioteca(s): Embrapa Gado de Leite; Embrapa Instrumentação. |
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| 167. | | CAMARGO, L. S. de A.; OTERO ARROYO, R. J.; ARAUJO, T. D.; QUINELATO, G. N.; QUINTAO, C. C. R.; IGUMA, L. T.; VIANA, J. H. M. Trichostatin a improves the in vitro development of cloned bovine embryos reconstructed with transgenic donor cells. In: ANNUAL CONFERENCE OF THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 2013, Hannover. Proceedings... Hannover: [s.n.], 2013.| Biblioteca(s): Embrapa Gado de Leite. |
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| 168. | | CAMARGO, L. S. de A.; QUINTAO, C. C. R.; IGUMA, L. T.; SIQUEIRA, L. G. B.; CARVALHO, B. C. de; VIANA, J. H. M. Trichostatin on production of genetically modified bovine embryos by somatic cell nuclear transfer. Animal Reproduction, v. 9, n. 3, 2012. Edição dos abstracts do 26º Animal Meeting of the Brazilian Embryo Technology Society, Foz do Iguaçu, 2012.| Biblioteca(s): Embrapa Gado de Leite. |
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| 169. | | SEQUETO, R. L.; ANUNCIAÇÃO, R. A.; CAMARGO, L. S. de A.; FONSECA, J. F. da; VIANA, J. H. M.; FERNANDES, C. A. C.; SIQUEIRA, L. G. B. Tratamento com Flunixin meglumine não previne efeitos do benzoato de estradiol sobre a função luteal durante a ressincronização precoce de vacas leiteiras. In: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE PIBIC/CNPQ, 26., 2022, Juiz de Fora. Anais... Juiz de Fora: Embrapa Gado de Leite, 2022. p. 49-53.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 170. | | SEQUETO, R. L.; ANUNCIAÇÃO, R. A.; CAMARGO, L. S. de A.; FONSECA, J. F. da; VIANA, J. H. M.; FERNANDES, C. A. C.; SIQUEIRA, L. G. B. Tratamento com Flunixin meglumine não previne efeitos do benzoato de estradiol sobre a função luteal durante a ressincronização precoce de vacas leiteiras. In: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE PIBIC/CNPQ, 26., 2022, Juiz de Fora. Anais... Juiz de Fora: Embrapa Gado de Leite, 2022. p. 49-53.| Biblioteca(s): Embrapa Gado de Leite. |
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| 171. | | PEREIRA, M. M.; RAPOSO, N. R. B.; CAMARGO, L. S. de A.; ARAUJO, T. D.; LADEIRA, M. DE S.; LADEIRA, L. O.; BRANDAO, H. de M. Transfecção gênica em fibroblastos bovinos utilizando nanotubos de carbono multicamadas. In: WORKSHOP DA REDE DE NANOTECNOLOGIA APLICADA AO AGRONEGÓCIO, 6., 2012, Fortaleza. Anais... São Carlos: Embrapa Instrumentação; Fortaleza: Embrapa Agroindústria Tropical, 2012.| Biblioteca(s): Embrapa Gado de Leite. |
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| 172. | | OLIVEIRA, C. S.; MARQUES, S. C. S.; GUEDES, P. H. E.; FEUCHARD, V. L.; CAMARGO, A. J. R.; FREITAS, C. de; CAMARGO, L. S. de A. Thermal-treatment protocol to induce thermotolerance in bovine embryos. Reproduction, Fertility and Development, v. 33, n. 7, p. 497-501, 2021.| Biblioteca(s): Embrapa Gado de Leite. |
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| 173. | | GUEDES, P. H. E.; OLIVEIRA, C. S.; DIAS, H. R. S.; FREITAS, C. de; CAMARGO, A. J. R.; SARAIVA, N. Z.; CAMARGO, L. S. de A. Ultrasound characteristics and pregnancy loss in bovine IVF-derived pregnancies. Livestock Science, v. 257, 104847, 2022.| Biblioteca(s): Embrapa Gado de Leite. |
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| 174. | | OLIVEIRA, C. S.; SARAIVA, N. Z.; LIMA, M. R.; SERAPIÃO, R. V.; OLIVEIRA, L. Z.; MARTINS, C. B.; VANTINI, R.; GARCIA, J. M.; CAMARGO, L. S. de A. Roles of cell death in sexual dimorphism during preimplantation development Animal Reproduction, v. 12, n. 3, p. 772, 2015. Edição dos proceedings do 29º Annual Meeting of the Brazilian Embryo Technology Society, 2015, Gramado.| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Gado de Leite. |
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| 175. | | FERREIRA, A. de M.; VIANA, J. H. M.; SÁ, W. F. de; CAMARGO, L. S. de A.; VERNEQUE, R. da S. Restrição alimentar e atividade ovariana luteal cíclica pós-parto em vacas Girolanda. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 35, n. 12, p. 2521-28, dez. 2000 Título em inglês: Low level of nutrition and ovarian luteal cyclic activity postpartum in crossbred (holstein x zebu) cows.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 176. | | FRANCO, M. M.; PELLEGRIN, A.; FIGUEIREDO, R. A.; GARCIA, A. R.; CAMARGO, L. S. de A.; AZEVEDO, H. C.; VIANA, J. H. M. Rede de inovação em reprodução animal: a experiencia da Embrapa na articulação de pesquisa em biotecnologia da reprodução. In: Reunião Anual da SBTE, 25., 2011, Cumbuco. Anais...| Biblioteca(s): Embrapa Gado de Leite. |
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| 177. | | PEREIRA, M. M.; COSTA, F. de Q.; OLIVEIRA, A. P.; SERAPIÃO, R. V.; MACHADO, M. A.; VIANA, J. H. M.; CAMARGO, L. S. de A. Quantificação de transcritos maternos em oócitos bovinos submetidos a diferentes condições de maturação. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 62, n. 6, p. 1394-1400, 2011.| Biblioteca(s): Embrapa Gado de Leite. |
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| 178. | | GOULART, I. L.; FERREIRA, A. M.; CAMARGO, L. S. de A.; SINEDINO, L. D. P.; DOURADO, A. P.; NOGUEIRA, L. A. G. Replacement of girolando herd with f1 embryo produced in vitro with oocytes from Gyr and holstein cows using semen. Acta Scientiae Veterinariae, v. 38, p. s755, 2010. Supl.2.
Edição dos resumos da 24a Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Porto de Galinhas, 2010.| Biblioteca(s): Embrapa Gado de Leite. |
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| 179. | | GOULART, I. L.; FERREIRA, A. M.; CAMARGO, L. S. de A.; SINEDINO, L. D. P.; DOURADO, A. P.; NOGUEIRA, L. A. G. Reposição de rebanho girolando com embriões F1 produzidos in vitro com oócitos de vacas gir e holandesas utilizando sêmen sexado. Acta Scientiae Veterinariae, v. 38, p. 378, 2010. Edição dos resumos da 24a Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Porto de Galinhas, 2010.| Biblioteca(s): Embrapa Gado de Leite. |
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| 180. | | CAMARGO, L. S. de A.; FERREIRA, A. DE M.; VIANA, J. H. M.; SA, W. F. DE; CARVALHO, B. C. de; IGUMA, L. T. Reprodução. In: CAMPOS, O. F. de; MIRANDA, J. E. C. de (ed.). Gado de leite: o produtor pergunta, a Embrapa responde. 3. ed. rev. ampl. Brasília, DF: Embrapa; Juiz de Fora: Embrapa Gado de Leite, 2012. p. 161-184. (Coleção 500 perguntas 500 respostas).| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 311 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
18/02/2014 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
ARASHIRO, E. K. N.; PALHÃO, M. P.; WOHLRES-VIANA, S.; SIQUEIRA, L. G. B.; CAMARGO, L. S. de A.; HENRY, M. R. J. M.; VIANA, J. H. M. |
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Afiliação: |
EDUARDO KENJI N. ARASHIRO, UFMG; MILLER PEREIRA PALHÃO, UNIFENAS; SABINE WOHLRES-VIANA, UFJF; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; MARC ROGER JEAN MARIE HENRY, UFMG; JOAO HENRIQUE MOREIRA VIANA, CNPGL. |
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Título: |
In vivo collection of follicular fluid and granulosa cells from individual follicles of different diameters in cattle by an adapted ovum pick-up system. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Reproductive Biology and Endocrinology, v. 11, article 73, 2013. |
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DOI: |
https://doi.org/10.1186/1477-7827-11-73 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Most studies on granulosa cell (GC) function in cattle have been performed using GC and follicular fluid (FF) samples collected from slaughterhouse ovaries. Using this approach, the follicular developmental stage and functional status are unknown and indirectly inferred, limiting data interpretation. Ultrasound-guided follicle aspiration has previously been used to recover GC or FF samples, but this was mostly carried out in large follicles or pools of small follicles, without recording the efficiency of recovery. The present study was aimed at adapting and evaluating an ovum pick-up (OPU) system for the in vivo recovery of FF and GC from individual follicles of different diameters. Methods: In the first trial, the losses of fluid inside the tubing system were calculated using a conventional or an adapted-OPU system. Blood plasma volumes equivalent to the amount of FF in follicles of different diameters were aspirated using a conventional OPU Teflon circuit. The OPU system was then adapted by connecting 0.25 mL straws to the circuit. A second trial evaluated the efficiency of FF recovery in vivo. Follicles ranging from 4.0 to 16.8 mm in diameter were aspirated individually using the conventional or adapted-OPU systems. A third trial assessed the in vivo recovery of GC and the subsequent amount of RNA obtained from the follicles of different diameters from Holstein and Gir cattle. Results: In Trial I, the plasma recovery efficiency was similar (P > 0.05) for the volumes expected for 12 and 10 mm follicles, but decreased (P < 0.05) for smaller follicles (45.7+/-4.0%, 12.4+/-4.3% and 0.0+/-0.0% for 8, 6, and 4 mm follicles, respectively). Using the adaptation, the losses intrinsic to the aspiration system were similar for all follicle diameters. In Trial II, the expected and recovered volumes of FF were correlated (r = 0.89) and the efficiency of recovery was similar among follicles <12 mm, while larger follicles had a progressive increase in FF losses that was not related to the tubing system. In Trial III, the number of GC and amount of RNA obtained were not affected (P > 0.05) by follicle size, but differed according to breed (615,054+/-58,122 vs 458,095+/-36,407 for Holstein and Gir, respectively; P < 0.05). Conclusions: The adapted-OPU system can be successfully used for the in vivo collection of FF and GC from follicles of different diameters. This will enable further endocrine, cellular, and gene expression analyses. MenosBackground: Most studies on granulosa cell (GC) function in cattle have been performed using GC and follicular fluid (FF) samples collected from slaughterhouse ovaries. Using this approach, the follicular developmental stage and functional status are unknown and indirectly inferred, limiting data interpretation. Ultrasound-guided follicle aspiration has previously been used to recover GC or FF samples, but this was mostly carried out in large follicles or pools of small follicles, without recording the efficiency of recovery. The present study was aimed at adapting and evaluating an ovum pick-up (OPU) system for the in vivo recovery of FF and GC from individual follicles of different diameters. Methods: In the first trial, the losses of fluid inside the tubing system were calculated using a conventional or an adapted-OPU system. Blood plasma volumes equivalent to the amount of FF in follicles of different diameters were aspirated using a conventional OPU Teflon circuit. The OPU system was then adapted by connecting 0.25 mL straws to the circuit. A second trial evaluated the efficiency of FF recovery in vivo. Follicles ranging from 4.0 to 16.8 mm in diameter were aspirated individually using the conventional or adapted-OPU systems. A third trial assessed the in vivo recovery of GC and the subsequent amount of RNA obtained from the follicles of different diameters from Holstein and Gir cattle. Results: In Trial I, the plasma recovery efficiency was similar (P > 0.05) for the v... Mostrar Tudo |
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Palavras-Chave: |
Bovine; Follicle aspiration; Follicle biopsy. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03310naa a2200241 a 4500
001 1980418
005 2024-02-05
008 2013 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1186/1477-7827-11-73$2DOI
100 1 $aARASHIRO, E. K. N.
245 $aIn vivo collection of follicular fluid and granulosa cells from individual follicles of different diameters in cattle by an adapted ovum pick-up system.$h[electronic resource]
260 $c2013
520 $aBackground: Most studies on granulosa cell (GC) function in cattle have been performed using GC and follicular fluid (FF) samples collected from slaughterhouse ovaries. Using this approach, the follicular developmental stage and functional status are unknown and indirectly inferred, limiting data interpretation. Ultrasound-guided follicle aspiration has previously been used to recover GC or FF samples, but this was mostly carried out in large follicles or pools of small follicles, without recording the efficiency of recovery. The present study was aimed at adapting and evaluating an ovum pick-up (OPU) system for the in vivo recovery of FF and GC from individual follicles of different diameters. Methods: In the first trial, the losses of fluid inside the tubing system were calculated using a conventional or an adapted-OPU system. Blood plasma volumes equivalent to the amount of FF in follicles of different diameters were aspirated using a conventional OPU Teflon circuit. The OPU system was then adapted by connecting 0.25 mL straws to the circuit. A second trial evaluated the efficiency of FF recovery in vivo. Follicles ranging from 4.0 to 16.8 mm in diameter were aspirated individually using the conventional or adapted-OPU systems. A third trial assessed the in vivo recovery of GC and the subsequent amount of RNA obtained from the follicles of different diameters from Holstein and Gir cattle. Results: In Trial I, the plasma recovery efficiency was similar (P > 0.05) for the volumes expected for 12 and 10 mm follicles, but decreased (P < 0.05) for smaller follicles (45.7+/-4.0%, 12.4+/-4.3% and 0.0+/-0.0% for 8, 6, and 4 mm follicles, respectively). Using the adaptation, the losses intrinsic to the aspiration system were similar for all follicle diameters. In Trial II, the expected and recovered volumes of FF were correlated (r = 0.89) and the efficiency of recovery was similar among follicles <12 mm, while larger follicles had a progressive increase in FF losses that was not related to the tubing system. In Trial III, the number of GC and amount of RNA obtained were not affected (P > 0.05) by follicle size, but differed according to breed (615,054+/-58,122 vs 458,095+/-36,407 for Holstein and Gir, respectively; P < 0.05). Conclusions: The adapted-OPU system can be successfully used for the in vivo collection of FF and GC from follicles of different diameters. This will enable further endocrine, cellular, and gene expression analyses.
653 $aBovine
653 $aFollicle aspiration
653 $aFollicle biopsy
700 1 $aPALHÃO, M. P.
700 1 $aWOHLRES-VIANA, S.
700 1 $aSIQUEIRA, L. G. B.
700 1 $aCAMARGO, L. S. de A.
700 1 $aHENRY, M. R. J. M.
700 1 $aVIANA, J. H. M.
773 $tReproductive Biology and Endocrinology$gv. 11, article 73, 2013.
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