| |
|
|
| Registros recuperados : 311 | |
| 261. |  | BRAGANÇA, G. M.; BATISTA, R. I. T. P.; FABJAN, J. M. G. de S.; ALFRADIQUE, V. A. P.; CÔRTES, L. R.; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDÃO, F. Z. FSH dose and strategy of administration during ovarian simulation alter the gene expression profile in ovine cumulus-oocyte complexes. In: ANNUAL MEETING OF THE BRAZILIAN EMBRYO TECNNOLOGY SOCIETY, 14., 2017, Cabo de Santo Agostinho. Proceedings... Cabo de Santo Agostinho: SBTE, 2017. p. 746.| Biblioteca(s): Embrapa Gado de Leite. |
|    |
| 262. | | BRAGANÇA, G. M.; BATISTA, R. I. T. P.; SOUZA FABJAN, J. M. G. de; ALFRADIQUE, V. A. P.; CORTÊS, L. R.; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDÃO, F. Z. FSH dose and strategy of administration during ovarian stimulation alter the gene expression profile in ovine cumulus-oocyte complexes. Animal Reprodroduction, v. 14, n. 3, p. 746, Jul./Sept. 2017. Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE), Cabo de Santo Agostinho, PE, Brazil, August 17 to 19, 2017.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
| |
| 263. | | VIREQUE, A. A.; CAMARGO, L. S. de A.; FERREIRA, E. M.; ROSA E SILVA, A. A. M.; WATANABE, Y. F.; BOS-MIKICH, A.; MARTINS, W. P.; CAMPOS, J. R.; NAVARRO, P. A. A. S.; FERRIANI, R. A. Embryonic development and steroidogenesis of bovine cumulus-oocyte complexes matured in a-mem medium supplemented with pva or pvp-40. Reproduction, Fertility and Development, v. 22, n. 1, 2010. Edição dos proceedings do Annual Conference of the International Embryo, 2010, Cordoba.| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 264. | | SILVA, I. O. e; VASCONCELOS, R. B.; CAETANO, J. V. O.; GULART, L. V. M.; CAMARGO, L. S. de A.; BÁO, S. N.; SILVA, A. A. M. R. e. Induction of reversible meiosis arrest of bovine oocytes using a two-step procedure under defined and nondefined conditions. Theriogenology, v. 75, n. 6, p. 1115-1124, 2011.| Biblioteca(s): Embrapa Gado de Leite. |
|  |
| 265. | | OLIVEIRA, C. S.; GUEDES, P. H. E.; MARQUES, S. C. S.; SILVA, J. V. G.; CAMARGO, A. J. R.; FREITAS, C. de; SERAPIÃO, R. V.; Saraiva, N. Z.; CAMARGO, L. S. de A. Induction of survival mechanisms to thermal stress in bovine embryos. Animal Reproduction, v. 15, n. 3, p. 481, 2018. Edição dos proceedings do 32º Annual Meeting of the Brazilian Embryo Technology Society, Florianópolis, 2018.| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 266. | | CARVALHO, B. C. de; VARAGO, F. C.; RUAS, J. R. M.; MOURA NETO, J. R. DE; COSTA, E. P. DA; VIANA, J. H. M.; CAMARGO, L. S. de A.; MENEZES, A. DE C. Indução de cio precoce em vacas no pós-parto para utilização de inseminação artificial. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 49., 2012, Brasília. A produção animal no mundo em transformação: anais. Brasília, DF: SBZ, 2012.| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 267. | | SOUZA, V. das G. P. de; SOUZA, G. T. de; LEMOS, D. R. de; GUIMARAES, J. M. de O.; QUINTAO, C. C. R.; MUNK, M.; SARAIVA, N. Z.; CAMARGO, L. S. de A. Heat shock during in vitro maturation of bovine oocytes disturbs bta-miR-19b and DROSHA transcripts abundance after in vitro fertilization. Reproduction in Domestic Animals, v. 56, n. 8, p. 1128-1136, 2021.| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 268. | | PEREIRA, M. M.; BRANDAO, H. de M.; QUINTAO, C. C. R.; SOUZA, E. D.; ARAÚJO, T. D.; LADEIRA, L. O.; RAPOSO, N. B. R.; CAMARGO, L. S. de A. Multiwalled carbon nanotubes vehicles for gene delivery into bovine embryos. Animal Reproduction, v. 11, n. 3, p. 478, 2014. Edição dos proceedings do 28º Annual Meeting of the Brazilian Embryo Technology Society, 2014, Natal.| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 269. | | LEMOS, D. R. de; SOUZA, J. F. da S. e; SOUZA, E. D. de; SARAIVA, N. Z.; QUINTAO, C. C. R.; SIQUEIRA, L. G. B.; OLIVEIRA, C. S.; CAMARGO, L. S. de A. Multiple mutations can be found in the exon 11 of prolactin receptor gene in crossbred bovine embryos. Animal Reproduction, v. 20, n. 2, 2023. Abstracts of 36th Annual Meeting of the Brazilian Embryo Technology Society (SBTE).| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 270. | | PEREIRA, M. M.; RAPOSO, N. R. B.; BRAYNER, R.; OLIVEIRA, V. de; QUINTÃO, C. C. R.; CAMARGO, L. S. de A.; MATTOSO, L. H. C.; BRANDÃO, H. de M. Nanofibras de celulose modulam a expressão de genes envolvidos na resposta ao estresse celular e apoptose em fibroblastos mamíferos. In: WORKSHOP DA REDE DE NANOTECNOLOGIA APLICADA AO AGRONEGÓCIO, 7.; ESCOLA DE NANOTECNOLOGIA, 3., 2013, São Carlos, SP. Anais... São Carlos, SP: Embrapa Instrumentação, 2013. p. 513-515 Editores: Maria Alice Martins, Odílio Benedito Garrido de Assis, Caue Ribeiro, Luiz Henrique Capparelli Mattoso. CD-ROM. Editores: Maria Alice Martins, Odílio Benedito Garrido de Assis, Caue Ribeiro, Luiz Henrique Capparelli Mattoso.| Biblioteca(s): Embrapa Gado de Leite; Embrapa Instrumentação. |
| |
| 271. | | OLIVEIRA, C. S.; ROSA, P. M. da S.; SARAIVA, H. F. R. de A.; FREITAS, C. de; CAMARGO, A. J. dos R.; QUINTAO, C. C. R.; SERAPIÃO, R. V.; CAMARGO, L. S. de A. Influência do estádio de desenvolvimento na viabilidade de embriões bovinos Gir/Holandês submetidos à biópsia embrionária para genotipagem. In: CONGRESSO INTERNACIONAL DO LEITE, 13., 2015, Porto Alegre. Anais... Juiz de Fora: Embrapa Gado de Leite, 2015. 4 p.| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 272. | | BATISTA, R. I. T. P.; RAPOSO, N. R. B.; CAMPOS JUNIOR, P. H. A.; PEREIRA, M. M.; CAMARGO, L. S. de A.; CARVALHO, B. C. de; GAMA, M. A. S. da; VIANA, J. H. M. Trans-10, cis-12 conjugated linoleic acid reduces neutral lipid content and may affect cryotolerance of in vitro-produced crossbred bovine embryos. Journal of Animal Science and Biotechnology, v. 5, n. 1, 2014. 8 p.| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 273. | | PEREIRA, M. M.; ASSUNÇÃO, C. M.; SERAPIAO, R. V.; CAMPOS JR. P. H. A.; COSTA, F. Q.; VIANA, J. H. M.; MACHADO, M. A.; CAMARGO, L. S. de A. Transcript analysis of maternal effect and oxidative stress genes in oocytes matured either in vivo or in vitro. In: CONGRESSO BRASILEIRO DE GENÉTICA, 55., 2009, Aguas de Lindoia. Anais... Águas de Lindoia: SBG, 2009.| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 274. | | PEREIRA, M. M.; QUINTAO, C. C. R.; BRANDAO, H. de M.; RAPOSO, N. B. R.; BRITO, S. G.; VIANA, J. H. M.; LADEIRA, L. O.; CAMARGO, L. S. de A. Toxicidade de nanotubos de carbono em fibroblastos bovinos. Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, 25., 2011, Cumbuco. Resumos... p. 441| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 275. | | PEREIRA, M. M.; BRANDAO, H. de M.; ZANETTE, R. de S. S.; QUINTAO, C. C. R.; SOUZA, E. D. De; ALMEIDA, L. B. F.; LADEIRA, L. O.; CAMARGO, L. S. de A. Transfecção gênica de células primárias de fibroblastos bovinos utilizando nanotubos de carbono multicamadas carboxilados. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 30., 2016, Foz do Iguaçu. Anais... Foz do Iguaçu: Sociedade Brasileira de Tecnologia de Embriões, 2016.| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 276. | | POLISSENI, J.; SERAPIÃO, R. V.; CAMPOS JR. P. H. A.; GRAZIA, J. G. V. DE; MILEN, L. C.; CAMARGO, L. S. de A.; GUERRA, M. DE O.; PETERS, V. M. Tecnica de TUNEL em embriões de ratas Wistar: avaliação da qualidade e da capacidade de desenvolvimento dos embriões. Revista Interdisciplinar de Estudos Experimentais, v. 1, n. 3, p. 110-113, 2010.| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 277. | | ARAUJO, T. D.; QUINTAO, C. C. R.; IGUMA, L. T.; CARVALHO, B. C. de; BARRETO, D. K.; VIANA, J. H. M.; ANUNCIAÇÃO, M. G.; MARANDUBA, C. P.; CAMARGO, L. S. de A. Use of commercial or handmade well of well (wow) dishes for in vitro production of bovine embryos. In: ANNUAL MEETING OF THE BRAZILIAN EMBRYO TECHNOLOGY SOCIETY, 28., 2014, Natal. Proceedings... [S.l.: s.n.], 2014. p. 445.| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 278. | | HALLERMAN, E. M.; BREDLAU, J. P.; CAMARGO, L. S. de A.; DAGLI, M. L. Z.; KAREMBU, M.; NGURE, G.; ROMERO-ALDEMITA, R.; ROCHA-SALAVARRIETA, P. J.; TIZARD, M.; WALTON, M.; WRAY-CAHEN, D. Towards progressive regulatory approaches for agricultural applications of animal biotechnology. Transgenic Research, v. 31, n. 2, p. 167-199, 2022.| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 279. | | LOPES, F. G.; COSTA, E. P. DA; QUEIROZ-CASTRO, V. L. D.; PEREIRA, E. C. M.; GUIMARÃES, J. D.; ALVES, S. V. P.; FERNANDES, C. A. C.; CAMARGO, L. S. de A.; BENJAMIM, L. DOS A. Use of two new formulations as bovine embryo manipulation solution. Animal Reproduction, v. 16 n. 2, p. 348-355, 2019.| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 280. | | MUNK, M.; ZANETTE, R. de S. S.; CAMARGO, L. S. de A.; SOUZA, N. L. G. D. de; ALMEIDA, C. G. de; GERN, J. C.; GUIMARAES, A. S.; LADEIRA, L. O.; OLIVEIRA, L. F. C. de; BRANDAO, H. de M. Using carbon nanotubes to deliver genes to hard-to-transfect mammalian primary fibroblast cells. Biomedical Physics & Engineering Express, v. 3, n. 4, 045002, 2017.| Biblioteca(s): Embrapa Gado de Leite. |
| |
| Registros recuperados : 311 | |
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Gado de Leite. |
|
Data corrente: |
26/04/2010 |
|
Data da última atualização: |
15/02/2024 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 2 |
|
Autoria: |
VIREQUE, A. A.; CAMARGO, L. S. de A.; SERAPIÃO, R. V.; ROSA E SILVA, A. A. M.; WATANABE, Y. F.; FERREIRA, E. M.; NAVARRO, P. A. A. S.; MARTINS, W. P.; FERRIANI, R. A. |
|
Afiliação: |
A. A. VIREQUE, USP; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; RAQUEL VARELLA SERAPIÃO, UFRRJ; A. A. M. ROSA E SILVA, Universidade de Brasília; Y. G. WATANABE, Vitrogen; E. M. FERREIRA, USP; P. A. A. S. NAVARRO, USP; W. P. MARTINS, USP; R. A. FERRIANI, USP. |
|
Título: |
Preimplantation development and expression of Hsp-70 and Bax genes in bovine blastocysts derived from oocytes matured in alpha-MEM supplemented with growth factors and synthetic macromolecules. |
|
Ano de publicação: |
2009 |
|
Fonte/Imprenta: |
Theriogenology, v. 71, n. 4, p. 620-627, 2009. |
|
DOI: |
https://doi.org/10.1016/j.theriogenology.2008.09.028 |
|
Idioma: |
Inglês |
|
Conteúdo: |
In vitro culture conditions affect both the maternal and embryonic expression of genes and is likely to alter both oocyte and embryo developmental competence. The search for better and less variable culture conditions simulating those in vivo has led to the development of defined culture media, with lower impact on the molecular reprogramming of oocytes and embryos. We evaluated embryo development and relative abundance (RA) of Hsp-70 and Bax transcripts in bovine blastocysts produced from oocytes matured in a chemically defined IVM system with synthetic polymers. Immature cumulus oocyte complexes (COCs) were matured for 22-24h in alpha-MEM supplemented with IGF-1, insulin, 0.1% polyvinyl alcohol (PVA), or 0.1% polyvinylpyrrolidone (PVP), but without FSH or LH. The control group consisted of COCs matured in TCM plus FSH and 10% estrous cow serum. After fertilization, presumptive zygotes were co-cultured with cumulus cells until 224 h post-insemination. Total RNA was isolated from embryo pools, reverse transcribed into cDNA, and subjected to transcript analysis by real-time PCR. Cleavage rate was higher (P<0.05) for the control group (68.3%) than for the PVA (54.4%) and PVP-40 (58.3%) groups. Nevertheless, there was no difference among the PVA, PVP-40 and control groups in blastocyst or hatching rates. Similarly, no difference in relative abundance of Hsp-70 and Bax transcripts was detected in comparison to the control group. We inferred that bovine oocytes can be matured in serum- and gonadotrophin-free medium supplemented with PVA or PVP, enriched with IGF-I and insulin, without altering post-cleavage development and relative abundance of some genes associated with stress and apoptosis. MenosIn vitro culture conditions affect both the maternal and embryonic expression of genes and is likely to alter both oocyte and embryo developmental competence. The search for better and less variable culture conditions simulating those in vivo has led to the development of defined culture media, with lower impact on the molecular reprogramming of oocytes and embryos. We evaluated embryo development and relative abundance (RA) of Hsp-70 and Bax transcripts in bovine blastocysts produced from oocytes matured in a chemically defined IVM system with synthetic polymers. Immature cumulus oocyte complexes (COCs) were matured for 22-24h in alpha-MEM supplemented with IGF-1, insulin, 0.1% polyvinyl alcohol (PVA), or 0.1% polyvinylpyrrolidone (PVP), but without FSH or LH. The control group consisted of COCs matured in TCM plus FSH and 10% estrous cow serum. After fertilization, presumptive zygotes were co-cultured with cumulus cells until 224 h post-insemination. Total RNA was isolated from embryo pools, reverse transcribed into cDNA, and subjected to transcript analysis by real-time PCR. Cleavage rate was higher (P<0.05) for the control group (68.3%) than for the PVA (54.4%) and PVP-40 (58.3%) groups. Nevertheless, there was no difference among the PVA, PVP-40 and control groups in blastocyst or hatching rates. Similarly, no difference in relative abundance of Hsp-70 and Bax transcripts was detected in comparison to the control group. We inferred that bovine oocytes can be matured in ... Mostrar Tudo |
|
Palavras-Chave: |
Bovine oocytes; In vitro maturation. |
|
Thesaurus NAL: |
embryogenesis. |
|
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/711876/1/Preimplantation-development-and-expression-of-Hsp-70-and-Bax-genes-in-bovine-blastocysts.pdf
|
|
Marc: |
LEADER 02626naa a2200265 a 4500
001 1711876
005 2024-02-15
008 2009 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.theriogenology.2008.09.028$2DOI
100 1 $aVIREQUE, A. A.
245 $aPreimplantation development and expression of Hsp-70 and Bax genes in bovine blastocysts derived from oocytes matured in alpha-MEM supplemented with growth factors and synthetic macromolecules.
260 $c2009
520 $aIn vitro culture conditions affect both the maternal and embryonic expression of genes and is likely to alter both oocyte and embryo developmental competence. The search for better and less variable culture conditions simulating those in vivo has led to the development of defined culture media, with lower impact on the molecular reprogramming of oocytes and embryos. We evaluated embryo development and relative abundance (RA) of Hsp-70 and Bax transcripts in bovine blastocysts produced from oocytes matured in a chemically defined IVM system with synthetic polymers. Immature cumulus oocyte complexes (COCs) were matured for 22-24h in alpha-MEM supplemented with IGF-1, insulin, 0.1% polyvinyl alcohol (PVA), or 0.1% polyvinylpyrrolidone (PVP), but without FSH or LH. The control group consisted of COCs matured in TCM plus FSH and 10% estrous cow serum. After fertilization, presumptive zygotes were co-cultured with cumulus cells until 224 h post-insemination. Total RNA was isolated from embryo pools, reverse transcribed into cDNA, and subjected to transcript analysis by real-time PCR. Cleavage rate was higher (P<0.05) for the control group (68.3%) than for the PVA (54.4%) and PVP-40 (58.3%) groups. Nevertheless, there was no difference among the PVA, PVP-40 and control groups in blastocyst or hatching rates. Similarly, no difference in relative abundance of Hsp-70 and Bax transcripts was detected in comparison to the control group. We inferred that bovine oocytes can be matured in serum- and gonadotrophin-free medium supplemented with PVA or PVP, enriched with IGF-I and insulin, without altering post-cleavage development and relative abundance of some genes associated with stress and apoptosis.
650 $aembryogenesis
653 $aBovine oocytes
653 $aIn vitro maturation
700 1 $aCAMARGO, L. S. de A.
700 1 $aSERAPIÃO, R. V.
700 1 $aROSA E SILVA, A. A. M.
700 1 $aWATANABE, Y. F.
700 1 $aFERREIRA, E. M.
700 1 $aNAVARRO, P. A. A. S.
700 1 $aMARTINS, W. P.
700 1 $aFERRIANI, R. A.
773 $tTheriogenology$gv. 71, n. 4, p. 620-627, 2009.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Leite (CNPGL) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
