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| Registros recuperados : 327 | |
| 121. |  | QUINTAO, C. C. R.; PEREIRA, M. M.; RABELO, N. C.; ALONSO, J. R. T.; IGUMA, L. T.; VIANA, J. H. M.; CAMARGO, L. S. de A. Efeito da passagem celular na produção de embriões da raça gir pela técnica de transferência nuclear. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 25., 2011, Cumbuco. Anais... Jaboticabal: Sociedade Brasileira de Transferência de Embriões, 2011. p. 435.| Biblioteca(s): Embrapa Gado de Leite. |
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| 122. | | CAMARGO, L. S. de A.; SÁ, W. F. de; FERREIRA, A. de M.; VIANA, J. H. M.; ARAÚJO, M. C. C. Efeito de concentração espermática e período de incubação oócito-espematozóides na fecundação in vitro em bovinos de raça gir. Pesquisa Agropecária Brasileira, Brasília, v.37, n.5, p.709-715, 2002.| Biblioteca(s): Embrapa Gado de Leite. |
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| 123. | | CAMARGO, L. S. de A.; SÁ, W. F. de; FERREIRA, A. de M.; VIANA, J. H. M.; ARAÚJO, M. C. C. Efeito de concentração espermática e período de incubação oócito-espermatozóides na fecundação in vitro em bovinos da raça Gir. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 37, n. 5, p. 709-716, maio 2002 Título em inglês: Effect of sperm concentration and incubation period of oocytespermatozoa on in vitro fertilization in the Gir breed.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 124. | | ASSUNÇÃO, C. M.; GRAZIA, J. G. V.; CAMPOS JR. P. H. A.; PEREIRA, M. M.; AZEVEDO, A. L. S.; QUINTÃO, C. C.; CAMARGO, L. S. de A. Efeito da mitomicina C sobre a viabilidade e ciclo celular de fibroblasto adultos bovinos após diferentes tempos de exposição. Acta Scientiae Veterinariae, v. 38, p. 401, 2010. Edição dos resumos da 24a Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Porto de Galinhas, 2010.| Biblioteca(s): Embrapa Gado de Leite. |
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| 125. | | ASSUNÇÃO, C. M. de; AZEVEDO, A. L. S.; QUINTAO, C. C. R.; VIANA, J. H. M.; RAPOSO, N. R. B.; CAMARGO, L. S. de A. Efeito da mitomicina C sobre a viabilidade e ciclo celular de fibroblastos adultos bovinos após diferentes tempos de exposição. In: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE, 5., 2010, Juiz de Fora, MG. Anais... Juiz de Fora: Embrapa Gado de Leite, 2010. 1 CD-ROM. (Embrapa Gado de Leite. Documentos, 141.). p. 37-40.| Biblioteca(s): Embrapa Gado de Leite. |
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| 126. | | ARAUJO, T. D.; JASMIN, J.; QUINTAO, C. C. R.; SOUZA, E. D.; VIANA, J. H. M.; CAMARGO, L. S. de A. Effect of DNA methylation inhibitor on heterochromatin in bovine embryos derived from heat-shocked oocytes. In: ANNUAL CONFERENCE OF THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 2016, Louisville. Reproduction, fertility and development: proceedings. Louisville: [s.n.], 2016.| Biblioteca(s): Embrapa Gado de Leite. |
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| 128. | | VIEIRA, A. C.; QUINTAO, C. C. R.; SIQUEIRA, L. G. B.; OLIVEIRA, C. S.; SILVA, T. A.; VIEIRA, C. D.; CAMARGO, L. S. de A. Effect of epigenetic modulators on development of bovine embryos derived from heatshocked oocytes. Animal Reproduction, v. 15, n. 3, p. 483, 2018. Edição dos proceedings do 32º Annual Meeting of the Brazilian Embryo Technology Society, Florianópolis, 2018.| Biblioteca(s): Embrapa Gado de Leite. |
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| 129. | | BOLPATO, M. C. L.; SOUZA, E. D. de; GRÁZIA, J. G. V. de; ASSUNÇÃO, C. M. de; VIANA, J. H. M.; CAMARGO, L. S. de A. Efeito da raça e da estação do ano sobre a produção in vitro de embriões bovinos na região da Zona da Mata, MG. In: CONGRESSO INTERNACIONAL DO LEITE, 12.; WORKSHOP DE POLÍTICAS PÚBLICAS, 12.; SIMPÓSIO DE SUSTENTABILIDADE DA ATIVIDADE LEITEIRA, 13., 2013, Porto Velho. Anais... Brasília, DF: Embrapa, 2013. 3 p. 1 CD-ROM.| Biblioteca(s): Embrapa Gado de Leite. |
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| 130. | | PEREIRA, M. M.; COSTA, F. Q.; OLIVEIRA, A. P.; ASSUNÇÃO, C. M.; SERAPIÃO, R. V.; VIANA, J. H. M.; MACHADO, M. A.; CAMARGO, L. S. de A. Effect of bovine oocyte maturation system on relative abundance of mRNA. In: ANNUAL CONFERENCE OT THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 36., 2010, Cordoba. Proceedings... Córdoba: ITES, 2010.| Biblioteca(s): Embrapa Gado de Leite. |
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| 131. | | QUINTAO, C. C. R.; SOUZA, E. D.; OLIVEIRA, C. S.; FREITAS, C. de; OLIVEIRA, J. A. S.; CAMARGO, L. S. de A. Effect of handmade biopsy on bovine embryons at the sixth day post in vitro fertilization Animal Reproduction., v.12, n.3, Jul./Sept. 2015. Edição dos proceedings do 29º Annual Meeting of the Brazilian Embryo Technology Society, 2015, Gramado.| Biblioteca(s): Embrapa Gado de Leite. |
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| 133. | | COSTA, F. Q.; PEREIRA, M. M.; WOHLRES VIANA, S.; SERAPIAO, R. V.; CARVALHO, B. C.; VIANA, J. H. M.; NOGUEIRA, L. A. G.; CAMARGO, L. S. de A. Effect of heat stress during oocyte maturation on gene expression of in vitro-fertilized and parthenogenetic bovine blastocysts. In: ANNUAL CONFERENCE OT THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 36., 2010, Cordoba. Proceedings... Córdoba: ITES, 2010.| Biblioteca(s): Embrapa Gado de Leite. |
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| 134. | | VIANA, J. H. M.; VARGAS, M. S. B.; SIQUEIRA, L. G. B.; CAMARGO, L. S. de A.; FIGUEIREDO, A. C. S.; FERNANDES, C. A. C.; PALHÃO, M. P. Efficacy of induction of luteolysis in superovulated cows is dependent on time of prostaglandin F2alpha analog treatment: effects on plasma progesterone and luteinizing hormone profiles. Theriogenology, v. 86, n. 4, p. 934-939, 2016.| Biblioteca(s): Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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| 136. | | WOHLERS-VIANA, S.; BOITE, M. M.; PEREIRA, M. M.; SA, W. F.; VIANA, J. H. M.; MACHADO, M. A.; CAMARGO, L. S. de A. Effect of production system and breed on relative expression in bovine embryos. In: ANNUAL CONFERENCE OF THE INTERNATIONAL EMBRYO TRANSFER SOCIETY - REPRODUCTION, FERTILITY AND DEVELOPMENT., 21., 2009, San Diego. Proceedings... San Diego: Csiro, 2009. 1| Biblioteca(s): Embrapa Gado de Leite. |
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| 137. | | WOHLRES-VIANA, S.; BOITE, M. C.; PEREIRA, M. M.; SA, W. F. de; VIANA, J. H. M.; MACHADO, M. A.; CAMARGO, L. S. de A. Effect of production system and breed on relative expression in bovine embryos. In: ANNUAL CONFERENCE OF THE INTERNATIONAL EMBRYO TRANSFER SOCIETY - REPRODUCTION, FERTILITY AND DEVELOPMENT, 21., 2009, San Diego. Proceeding... San Diego: Csiro, 2009.| Biblioteca(s): Embrapa Gado de Leite. |
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| 138. | | PATROCÍNIOS. T. A.; FERNANDES, C. A. C.; AMORIM, L. S.; RIBEIRO. J. R.; MACEDO, G. C.; SILVA, A. D.; VIANA, J. H. M.; CAMARGO, L. S. de A. Effect of reservatrol analogue on development of in vitro-fertilized bovine embryos. Reproduction, Fertility and Development, v. 29, n. 1, p. 183, 2017. Edição dos proceedings da Annual Conference of the International Embryo Technology Society, Austin, 2017.| Biblioteca(s): Embrapa Gado de Leite. |
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| 139. | | ASCARI, I. J.; ALVES, N. G.; CAMARGO, L. S. de A.; JASMIN, J.; QUINTAO, C. C. R.; OLIVEIRA, J. A. S.; ARAÚJO, T. D. Effect of melatonin on DNA fragmentation and in vitro maturation of bovine oocytes subjected to heat shock Animal Reproduction., v.12, n.3, Jul./Sept. 2015. Edição dos proceedings do 29º Annual Meeting of the Brazilian Embryo Technology Society, 2015, Gramado.| Biblioteca(s): Embrapa Gado de Leite. |
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| 140. | | PEREIRA, M. M.; MACHADO, M. A.; COSTA, F. de Q.; SERAPIÃO, R. V.; VIANA, J. H. M.; CAMARGO, L. S. de A. Effect of oxygen tension and serum during IVM on developmental competence of bovine oocytes. Reproduction, Fertility and Development, v. 22, p. 1074-1082, 2010.| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 327 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
14/01/2020 |
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Data da última atualização: |
06/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
FONSECA, J. F. da; BATISTA, R. I. T. P.; TREVIZAN, J. T.; SOUZA-FABJAN, J. M. G.; BRANDÃO, F. Z.; CAMARGO, L. S. de A. |
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Afiliação: |
JEFERSON FERREIRA DA FONSECA, CNPC; Ribrio Ivan Tavares Pereira Batista; Juliane Teramachi Trevizan; Joanna Maria Gonçalves Souza-Fabjan; Felipe Zandonadi Brandão; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL. |
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Título: |
Goat incubator: can bovine oocytes be matured in the uterine horn of a goat? |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Semina: Ciências Agrárias, Londrina, v. 40, n. 6, supp. 3, p. 3789-3796, 2019. |
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DOI: |
10.5433/1679-0359.2019v40n6Supl3p3789 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract We used a goat as a live incubator, along with associated nonsurgical embryo transfer techniques, to perform ex situ (in vivo) maturation of bovine oocytes. Immature bovine cumulus-oocyte complexes (COCs) aspirated from 3-8 mm follicles from slaughterhouse ovaries were randomly split into two groups for in vitro (IVM; n = 38) and ex situ maturation (ESM; n = 40). IVM was performed for a period of 24 h at 38.5 ºC and with 5% CO2 in the air of maximum humidity. For ESM, a presynchronized nulliparous goat (12 months old) received 40 immature COCs in the uterine horn apiece, via the transcervical route. After 24 h the structures were retrieved through uterine flushing. Analyses of nuclear maturation and lipid quantification were performed on oocytes from both groups. Fluorescent intensity was compared using the Student?s t-test. Forty-seven percent of the structures were recovered after uterine flushing (19/40). The nuclear maturation rate was 94.5% (18/19) and 81.6% (31/38) for the ESM and IVM groups, respectively. In vitro-matured COCs contained more lipid droplets, expressed as a higher amount (p < 0.05) of emitted fluorescent light than ex situ-matured COCs (858 ± 73 vs. 550 ± 64 arbitrary fluorescence units, respectively). This is the first report to associate nonsurgical embryo transfer techniques and a goat as a live incubator for the maturation of bovine oocytes. We conclude that bovine oocytes can progress meiotically in the uterus horn of a goat and that transcervical transfer of bovine oocytes to a goat?s uterus could present an alternative to nuclear maturation. MenosAbstract We used a goat as a live incubator, along with associated nonsurgical embryo transfer techniques, to perform ex situ (in vivo) maturation of bovine oocytes. Immature bovine cumulus-oocyte complexes (COCs) aspirated from 3-8 mm follicles from slaughterhouse ovaries were randomly split into two groups for in vitro (IVM; n = 38) and ex situ maturation (ESM; n = 40). IVM was performed for a period of 24 h at 38.5 ºC and with 5% CO2 in the air of maximum humidity. For ESM, a presynchronized nulliparous goat (12 months old) received 40 immature COCs in the uterine horn apiece, via the transcervical route. After 24 h the structures were retrieved through uterine flushing. Analyses of nuclear maturation and lipid quantification were performed on oocytes from both groups. Fluorescent intensity was compared using the Student?s t-test. Forty-seven percent of the structures were recovered after uterine flushing (19/40). The nuclear maturation rate was 94.5% (18/19) and 81.6% (31/38) for the ESM and IVM groups, respectively. In vitro-matured COCs contained more lipid droplets, expressed as a higher amount (p < 0.05) of emitted fluorescent light than ex situ-matured COCs (858 ± 73 vs. 550 ± 64 arbitrary fluorescence units, respectively). This is the first report to associate nonsurgical embryo transfer techniques and a goat as a live incubator for the maturation of bovine oocytes. We conclude that bovine oocytes can progress meiotically in the uterus horn of a goat and that trans... Mostrar Tudo |
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Palavras-Chave: |
Bovine oocytes; COCs; Ex-situ maturation; In vitro maturation; Nonsurgical embryo transfer technique. |
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Thesaurus NAL: |
Goats. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/208736/1/Goat-incubator-can-bovine-oocytes.pdf
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Marc: |
LEADER 02452naa a2200265 a 4500
001 2118709
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008 2019 bl uuuu u00u1 u #d
024 7 $a10.5433/1679-0359.2019v40n6Supl3p3789$2DOI
100 1 $aFONSECA, J. F. da
245 $aGoat incubator$bcan bovine oocytes be matured in the uterine horn of a goat?$h[electronic resource]
260 $c2019
520 $aAbstract We used a goat as a live incubator, along with associated nonsurgical embryo transfer techniques, to perform ex situ (in vivo) maturation of bovine oocytes. Immature bovine cumulus-oocyte complexes (COCs) aspirated from 3-8 mm follicles from slaughterhouse ovaries were randomly split into two groups for in vitro (IVM; n = 38) and ex situ maturation (ESM; n = 40). IVM was performed for a period of 24 h at 38.5 ºC and with 5% CO2 in the air of maximum humidity. For ESM, a presynchronized nulliparous goat (12 months old) received 40 immature COCs in the uterine horn apiece, via the transcervical route. After 24 h the structures were retrieved through uterine flushing. Analyses of nuclear maturation and lipid quantification were performed on oocytes from both groups. Fluorescent intensity was compared using the Student?s t-test. Forty-seven percent of the structures were recovered after uterine flushing (19/40). The nuclear maturation rate was 94.5% (18/19) and 81.6% (31/38) for the ESM and IVM groups, respectively. In vitro-matured COCs contained more lipid droplets, expressed as a higher amount (p < 0.05) of emitted fluorescent light than ex situ-matured COCs (858 ± 73 vs. 550 ± 64 arbitrary fluorescence units, respectively). This is the first report to associate nonsurgical embryo transfer techniques and a goat as a live incubator for the maturation of bovine oocytes. We conclude that bovine oocytes can progress meiotically in the uterus horn of a goat and that transcervical transfer of bovine oocytes to a goat?s uterus could present an alternative to nuclear maturation.
650 $aGoats
653 $aBovine oocytes
653 $aCOCs
653 $aEx-situ maturation
653 $aIn vitro maturation
653 $aNonsurgical embryo transfer technique
700 1 $aBATISTA, R. I. T. P.
700 1 $aTREVIZAN, J. T.
700 1 $aSOUZA-FABJAN, J. M. G.
700 1 $aBRANDÃO, F. Z.
700 1 $aCAMARGO, L. S. de A.
773 $tSemina: Ciências Agrárias, Londrina$gv. 40, n. 6, supp. 3, p. 3789-3796, 2019.
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Embrapa Gado de Leite (CNPGL) |
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