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281. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | PEREIRA, M. M.; ASSUNÇÃO, C. M.; SERAPIAO, R. V.; CAMPOS JR. P. H. A.; COSTA, F. Q.; VIANA, J. H. M.; MACHADO, M. A.; CAMARGO, L. S. de A. Transcript analysis of maternal effect and oxidative stress genes in oocytes matured either in vivo or in vitro. In: CONGRESSO BRASILEIRO DE GENÉTICA, 55., 2009, Aguas de Lindoia. Anais... Águas de Lindoia: SBG, 2009. Biblioteca(s): Embrapa Gado de Leite. |
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282. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | PEREIRA, M. M.; QUINTAO, C. C. R.; BRANDAO, H. de M.; RAPOSO, N. B. R.; BRITO, S. G.; VIANA, J. H. M.; LADEIRA, L. O.; CAMARGO, L. S. de A. Toxicidade de nanotubos de carbono em fibroblastos bovinos. Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, 25., 2011, Cumbuco. Resumos... p. 441 Biblioteca(s): Embrapa Gado de Leite. |
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283. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | PEREIRA, M. M.; BRANDAO, H. de M.; ZANETTE, R. de S. S.; QUINTAO, C. C. R.; SOUZA, E. D. De; ALMEIDA, L. B. F.; LADEIRA, L. O.; CAMARGO, L. S. de A. Transfecção gênica de células primárias de fibroblastos bovinos utilizando nanotubos de carbono multicamadas carboxilados. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 30., 2016, Foz do Iguaçu. Anais... Foz do Iguaçu: Sociedade Brasileira de Tecnologia de Embriões, 2016. Biblioteca(s): Embrapa Gado de Leite. |
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284. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | HALLERMAN, E. M.; BREDLAU, J. P.; CAMARGO, L. S. de A.; DAGLI, M. L. Z.; KAREMBU, M.; NGURE, G.; ROMERO-ALDEMITA, R.; ROCHA-SALAVARRIETA, P. J.; TIZARD, M.; WALTON, M.; WRAY-CAHEN, D. Towards progressive regulatory approaches for agricultural applications of animal biotechnology. Transgenic Research, v. 31, n. 2, p. 167-199, 2022. Biblioteca(s): Embrapa Gado de Leite. |
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285. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | POLISSENI, J.; MACHADO, M. A.; AZEVEDO, A. L. S.; DOMINGUES, R.; GUERRA, M. O.; SERAPIÃO, R. V.; PEREIRA, M. M.; SÁ, W. F. DE; CARVALHO, B. C.; CAMARGO, L. S. de A.; VIANA, J. H. M.; PETERS, V. M. Whole genome amplification on blastomers of Post-Biopsy Bovine embryo. In: ANNUAL CONFERENCE OF THE INTERNATIONAL EMBRYO TRANSFER SOCIETY - REPRODUCTION, FERTILITY AND DEVELOPMENT, 21., 2009, San Diego. Proceedings... San Diego: IETS, 2009. Biblioteca(s): Embrapa Gado de Leite. |
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286. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | WOHLRES-VIANA, S.; PEREIRA, M. M.; SALES, J. N. S.; CAMARGO, J. DOS R.; QUINTAO, C. C. R.; RABELO, N. C.; DENICOL, A. C.; SIQUEIRA, L. G. B.; VIANA, J. H. M.; CAMARGO, L. S. de A.; MARTINS, M. F.; IGUMA, L. T. Alteraçãoda expressão de genes relacionados com o metabolismo de glicose em oócitos bovinos maturados in vitro ou in vivo. In: Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, 25., 2011, Cumbuco. Resumos... p. 443 Biblioteca(s): Embrapa Gado de Leite. |
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287. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | ACARI, I. J.; ALVES, N. G.; JASMIN, J.; QUINTAO, C. C. R.; SOUZA, J. F.; TORRES, G.; GONÇALVES, F.; JUNQUEIRA, F. B.; SILVA, D. R.; CAMPOS, J. P. A.; FARIA, L. R.; PAULA, F. A. De P.; CAMARGO, L. S. de A. Antioxidant effects of IGF-I on bovine oocytes subjected to heat shock In: INTERNATIONAL CONGRESS ON ANIMAL REPRODUCTION, 18., 2016, Tours. Abstracts... Tours: Society for Reproductive Biology, 2016. p. 246-247. Biblioteca(s): Embrapa Gado de Leite. |
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288. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | WOHLRES-VIANA, S.; PEREIRA, M. M.; SALES, J. N. S.; CAMARGO, A. J. DOS R.; QUINTAO, C. C. R.; RABELO, N. C.; DENICOL, A. C.; SIQUEIRA, L. G. B.; VIANA, J. H. M.; CAMARGO, L. S. de A.; MARTINS, M. F.; IGUMA, L. T. Altered expression of genes related to glucose metabolism in bovine oocytes matured in vitro or in vivo. In: Annual Meeting SBTE, 25., 2011. Abstracts... Biblioteca(s): Embrapa Gado de Leite. |
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289. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | BRAGANÇA, G. M.; BATISTA, R. I. T. P.; SOUZA-FABJAN, J. M. G.; ALFRADIQUE, V. A. P.; ARASHIRO, E. K. N.; PINTO, P. H. N.; SANTOS, J. D. R.; CAMARGO, L. S. de A.; MENCHACA, A.; FONSECA, J. F. da; BRANDÃO, F. Z. Exogenous progestogens differentially alter gene expression of immature cumulus-oocyte complexes in sheep. Domestic Animal Endocrinology, v. 74, e106518, 2021. Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
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290. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | FOLHADELLA, I. M.; CASTRO, T. S.; SALVADOR, D. F.; SÁ, W. F.; FERREIRA, A. M.; RAMOS, A. A.; POLISSENI, J.; SERAPIÃO, R. V.; CAMARGO, L. S. de A.; ANDRADE, V. J.; SANTORO, M. M.; OLIVEIRA, J. S.; RODRIGUES, L. H.; VALE FILHO, V. R. Identificação de proteínas do plasma seminal de touros da raça Gir. Revista Brasileira de Reprodução Animal, v. 37, n. 1, p. 64-71, 2013. Biblioteca(s): Embrapa Gado de Leite. |
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291. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | PAULA, F. B. E. de; SOUZA, E. D.; QUINTAO, C. C. R.; ANUNCIAÇÃO, M. G. da; ARAÚJO, T. D.; VIANA, J. H. M.; IGUMA, L. T.; CARVALHO, B. C. de; TOLEDO ALONSO, J. R.; SÁNCHEZ, O.; PARRA, N. C.; CAMARGO, L. S. de A. Effect of recombinant human FSH during in vitro maturation on apoptose of bovine blastocysts. Animal Reproduction, v.10, n.3, p. 518, Jul./Sept. 2013. Biblioteca(s): Embrapa Gado de Leite. |
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292. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | MAIA, G. G.; SIQUEIRA, L. G. B.; VASCONCELOS, C. O. de P.; TOMICH, T. R.; CAMARGO, L. S. de A.; RODRIGUES, J. P. P.; MENEZES, R. A. de; GONÇALVES, L. C.; TEIXEIRA, B. F.; GRANDO, R. de O.; NOGUEIRA, L. A. G.; PEREIRA, L. G. R. Effects of heat stress on rumination activity in Holstein-Gyr dry cows. Livestock Science, v. 239, 104092, 2020. Biblioteca(s): Embrapa Gado de Leite. |
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293. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | SALES, J. N. S.; IGUMA, L. T.; BATISTA, R. I. T. P.; QUINTAO, C. C. R.; GAMA, M. A. S. da; FREITAS, C. de; PEREIRA, M. M.; CAMARGO, L. S. de A.; VIANA, J. H. M.; SOUZA, J. C.; BARUSELLI, P. S. Effects of a high-energy diet on oocyte quality and in vitro embryo production in Bos indicus and Bos taurus cows. Journal of Dairy Science, v. 98, n. 5, p. 3086-3099, 2015. Biblioteca(s): Embrapa Gado de Leite. |
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294. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | PAES, V. M.; VIEIRA, L. A.; CORREIA, H. H. V.; SA, N. A. R.; MOURA, A. A. A.; SALES, A. D.; RODRIGUES, A. P. R.; MAGALHÃES-PADILHA, D. M.; SANTOS, F. W.; APGAR, G. A.; CAMPELLO, C. C.; CAMARGO, L. S. de A.; FIGUEIREDO, J. R. Effect of heat stress on the survival and development of in vitro cultured bovine preantral follicles and on in vitro maturation of cumulus-oocyte complex. Theriogenology, n. 86, p. 994-1003, 2016. Biblioteca(s): Embrapa Gado de Leite. |
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295. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | COMINATO, V.; ALBUQUERQUE, B. S. F.; PEREIRA, B. P.; RIBEIRO, A. K. do C.; SOARES, G. O.; PAIXÃO, R.; MAIA, G. G.; SIQUEIRA, L. G. B.; CAMARGO, L. S. de A.; PEREIRA, L. G. R.; CAMPOS, M. M.; TOMICH, T. R. Efeitos do estresse térmico na atividade da ruminação e na degradabilidade ruminal in situ em vacas leiteiras. In: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE, 25., 2021, Juiz de Fora. Anais... Juiz de Fora: Embrapa Gado de Leite, 2021. (Embrapa Gado de Leite. Documentos, 261). Biblioteca(s): Embrapa Gado de Leite. |
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296. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | QUINTAO, C. C. R.; VIANA, J. H. M.; SALES, J. N. S.; BATISTA, R. I. T. P.; PEREIRA, M. M.; OLIVEIRA, A. P.; CARVALHO, B. C.; CAMPOS JR. P. H.; SERAPIÃO, R. V.; FREITAS, C. de; CAMARGO, L. S. de A.; VERNEQUE, R. da S.; IGUMA, L. T. Comparação da taxa de prenhez inicial de embriões produzidos in vitro entre receptoras novilhas e vacas. Acta Scientiae Veterinariae, v. 38, p. 384, 2010. Edição dos resumos da 24a Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Porto de Galinhas, 2010. Biblioteca(s): Embrapa Gado de Leite. |
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297. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | SARAIVA, H. F. R. de A.; BATISTA, R. I. T. P.; ALFRADIQUE, V. A. P.; PINTO, P. H. N.; RIBEIRO, L. S.; OLIVEIRA, C. S.; SOUZA FABJAN, J. M. G. de; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDÃO, F. Z. L-carnitine supplementation during vitrification did not improve survival and quality rates, but altered CrAT and PRDX1 expression in in vivo-produced ovine embryos. Animal Reprodroduction, v. 14, n. 3, p. 898, Jul./Sept. 2017. Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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298. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | SARAIVA, H. F. R. de A.; BATISTA, R. I. T. P.; ALFRADIQUE, V. A. P.; PINTO, P. H. N.; RIBEIRO, L. S.; OLIVEIRA, C. S.; SOUZA-FABJAN, J. M. G. de; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDÃO, F. Z. L-carnitine supplementation during vitrification did not improve survival and quality rates, but altered CrAT and PRDXI expression in in vivo-produced ovine embryos. In: ANNUAL MEETING OF THE BRAZILIAN EMBRYO TECNNOLOGY SOCIETY, 14., 2017, Cabo de Santo Agostinho. Proceedings... Cabo de Santo Agostinho: SBTE, 2017. p. 901. Biblioteca(s): Embrapa Gado de Leite. |
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299. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | SARAIVA, H. F. R. de A.; BATISTA, R. I. T. P.; ALFRADIQUE, V. A. P.; PINTO, P. H. N.; RIBEIRO, L. S.; OLIVEIRA, C. S.; SOUZA-FABJAN, J. M. G. de; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDÃO, F. Z. l-carnitine supplementation during vitrification or warming of in vivo-produced ovine embryos does not affect embryonic survival rates, but alters CrAT and PRDX1 expression. Theriogenology, v. 105, p. 150-157, Jan. 2018. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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300. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | SARAIVA, H. F. R. de A.; BATISTA, R. I. T. P.; ALFRADIQUE, V. A. P.; PINTO, P. H. N.; RIBEIRO, L. S.; OLIVEIRA, C. S.; SOUZA-FABJAN, J. M. G. de; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDAO, F. Z. L-carnitine supplementation during vitrification or warming of in vivo-produced ovine embryos does not affect embryonic survival rates, but alters CrAT and PRDXI expression. Theriogenology, v. 105, p. 150-157, jan. 2018. Biblioteca(s): Embrapa Gado de Leite. |
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Registros recuperados : 311 | |
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Registro Completo
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
24/08/2017 |
Data da última atualização: |
23/09/2019 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
SARAIVA, H. F. R. de A.; BATISTA, R. I. T. P.; ALFRADIQUE, V. A. P.; PINTO, P. H. N.; RIBEIRO, L. S.; OLIVEIRA, C. S.; SOUZA FABJAN, J. M. G. de; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDÃO, F. Z. |
Afiliação: |
Helena Fabiana Reis de Almeida Saraiva, Universidade de São Paulo (USA) - Pirassununga, SP, Brasil; Ribrio Ivan Tavares Pereira Batista, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brasil; Vivian Angélico Pereira Alfradique, UFF - Niterói, RJ, Brasil; Pedro Henrique Nicolau Pinto, UFF - Niterói, RJ, Brasil; Lilian Santos Ribeiro, UFF - Niterói, RJ, Brasil; CLARA SLADE OLIVEIRA, CNPGL; Joanna Maria Gonçalves de Souza Fabjan, UFF - Niterói, RJ, Brasil; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; JEFERSON FERREIRA DA FONSECA, CNPC; Felipe Zandonadi Brandão, UFF - Niterói, RJ, Brasil. |
Título: |
L-carnitine supplementation during vitrification did not improve survival and quality rates, but altered CrAT and PRDX1 expression in in vivo-produced ovine embryos. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Animal Reprodroduction, v. 14, n. 3, p. 898, Jul./Sept. 2017. |
Idioma: |
Inglês |
Notas: |
Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts. |
Conteúdo: |
Embryo cryodamage is observed mainly at metabolic and molecular aspects and it impairs post warming quality and survival rates. This study aimed to evaluate the effect of L-carnitine (LC) supplementation during either vitrification or post warming solutions on the 6-7th day of in vivo-produced ovine embryos. LC (3.72 mM) was added to vitrification (Experiment 1; C1: control; LC1: supplemented embryos) or warming solutions (Experiment 2; C2; LC2). In vitro culture (IVC) of warmed embryos was performed for 72 h at 38,5 °C, 5% CO2 and 5% O2 to evaluate survival rates in both Experiments. In Experiment 1, reactive oxygen species (ROS) levels were measured by CellROX Green staining, total cell number (TCN) by Hoechst 33342, number of apoptotic cells by caspase-3 immunofluorescence staining protocol, apoptotic index evaluation in both groups. Gene expression analysis of carnitine palmitoyltransferase 1 and 2 (CPT1 and CPT2), carnitine O-acyltransferase (CrAT) and peroxiredoxin 1 (PRDX1), were performed by RT-qPCR (ACTB as endogenous control) in Experiments 1 and 2 and results were compared to fresh embryos (FE). Averages of survival rates were compared by the Chi-Square test. Means of TCN, apoptotic cells, apoptotic index and fluorescence intensity were compared by Student's t-test, at 5% significance level. Survival rates were similar between groups (p> 0.05) in Experiments 1 (68.7%, C1 vs 81.8%, LC1) and 2 (48.5%, C2 vs 64.7%, LC2). In Experiment 1, ROS levels at 24 h of IVC (85.83 ± 68.37 x 1010, C1 vs 89.04 ± 84.48 x 1010, LC1), total cell number at 24 h (89 ± 22, C1 vs 82.2 ± 28, LC1) and 72 h (86 ± 19.9, C1 vs 68.5 ± 25.26, LC1), apoptotic cells (3.75 ± 1.48, C1 vs 4.50 ± 4.72, LC1) and apoptotic index (4.37 ± 1.45, C1 vs 5.23 ± 4.72, LC1) at 72 h of IVC did not differ (p> 0.05) between C1 and LC1. Gene expression analysis showed no differences in CPT1 and CPT2 mRNA relative abundance in embryos of both experiments compared to FE, however, CrAT was downregulated (p< 0.05) in C1 and PRDX1 was downregulated (p< 0.05) in both C1 and LC1, compared to FE. Moreover, CrAT and PRDX1 were upregulated (p< 0.05) in C2 and CrAT was downregulated (p< 0.05) in LC2, in relation to FE. In conclusion, although the short-term LC supplementation at 3.72 mM during cryopreservation did not improve post-warming survival and morphological parameters of the evaluated embryos, it was able to modulate expression of genes related to energy homeostasis (CrAT) and oxidative stress (PRDX1), proving to be beneficial, in both forms of supplementation, to in vivo-produced ovine embryos.". MenosEmbryo cryodamage is observed mainly at metabolic and molecular aspects and it impairs post warming quality and survival rates. This study aimed to evaluate the effect of L-carnitine (LC) supplementation during either vitrification or post warming solutions on the 6-7th day of in vivo-produced ovine embryos. LC (3.72 mM) was added to vitrification (Experiment 1; C1: control; LC1: supplemented embryos) or warming solutions (Experiment 2; C2; LC2). In vitro culture (IVC) of warmed embryos was performed for 72 h at 38,5 °C, 5% CO2 and 5% O2 to evaluate survival rates in both Experiments. In Experiment 1, reactive oxygen species (ROS) levels were measured by CellROX Green staining, total cell number (TCN) by Hoechst 33342, number of apoptotic cells by caspase-3 immunofluorescence staining protocol, apoptotic index evaluation in both groups. Gene expression analysis of carnitine palmitoyltransferase 1 and 2 (CPT1 and CPT2), carnitine O-acyltransferase (CrAT) and peroxiredoxin 1 (PRDX1), were performed by RT-qPCR (ACTB as endogenous control) in Experiments 1 and 2 and results were compared to fresh embryos (FE). Averages of survival rates were compared by the Chi-Square test. Means of TCN, apoptotic cells, apoptotic index and fluorescence intensity were compared by Student's t-test, at 5% significance level. Survival rates were similar between groups (p> 0.05) in Experiments 1 (68.7%, C1 vs 81.8%, LC1) and 2 (48.5%, C2 vs 64.7%, LC2). In Experiment 1, ROS levels at 24 h of IVC (85... Mostrar Tudo |
Palavras-Chave: |
Animal embryos; Santa Inês; Survival. |
Thesaurus NAL: |
Carnitine; Cell culture; Cryopreservation; Ewes; Gene expression; In vitro culture; Sheep; Vitrification. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/163011/1/CNPC-2017-L-carnitine.pdf
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Marc: |
LEADER 03898nam a2200361 a 4500 001 2074433 005 2019-09-23 008 2017 bl uuuu u00u1 u #d 100 1 $aSARAIVA, H. F. R. de A. 245 $aL-carnitine supplementation during vitrification did not improve survival and quality rates, but altered CrAT and PRDX1 expression in in vivo-produced ovine embryos.$h[electronic resource] 260 $aAnimal Reprodroduction, v. 14, n. 3, p. 898, Jul./Sept. 2017.$c2017 500 $aProceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts. 520 $aEmbryo cryodamage is observed mainly at metabolic and molecular aspects and it impairs post warming quality and survival rates. This study aimed to evaluate the effect of L-carnitine (LC) supplementation during either vitrification or post warming solutions on the 6-7th day of in vivo-produced ovine embryos. LC (3.72 mM) was added to vitrification (Experiment 1; C1: control; LC1: supplemented embryos) or warming solutions (Experiment 2; C2; LC2). In vitro culture (IVC) of warmed embryos was performed for 72 h at 38,5 °C, 5% CO2 and 5% O2 to evaluate survival rates in both Experiments. In Experiment 1, reactive oxygen species (ROS) levels were measured by CellROX Green staining, total cell number (TCN) by Hoechst 33342, number of apoptotic cells by caspase-3 immunofluorescence staining protocol, apoptotic index evaluation in both groups. Gene expression analysis of carnitine palmitoyltransferase 1 and 2 (CPT1 and CPT2), carnitine O-acyltransferase (CrAT) and peroxiredoxin 1 (PRDX1), were performed by RT-qPCR (ACTB as endogenous control) in Experiments 1 and 2 and results were compared to fresh embryos (FE). Averages of survival rates were compared by the Chi-Square test. Means of TCN, apoptotic cells, apoptotic index and fluorescence intensity were compared by Student's t-test, at 5% significance level. Survival rates were similar between groups (p> 0.05) in Experiments 1 (68.7%, C1 vs 81.8%, LC1) and 2 (48.5%, C2 vs 64.7%, LC2). In Experiment 1, ROS levels at 24 h of IVC (85.83 ± 68.37 x 1010, C1 vs 89.04 ± 84.48 x 1010, LC1), total cell number at 24 h (89 ± 22, C1 vs 82.2 ± 28, LC1) and 72 h (86 ± 19.9, C1 vs 68.5 ± 25.26, LC1), apoptotic cells (3.75 ± 1.48, C1 vs 4.50 ± 4.72, LC1) and apoptotic index (4.37 ± 1.45, C1 vs 5.23 ± 4.72, LC1) at 72 h of IVC did not differ (p> 0.05) between C1 and LC1. Gene expression analysis showed no differences in CPT1 and CPT2 mRNA relative abundance in embryos of both experiments compared to FE, however, CrAT was downregulated (p< 0.05) in C1 and PRDX1 was downregulated (p< 0.05) in both C1 and LC1, compared to FE. Moreover, CrAT and PRDX1 were upregulated (p< 0.05) in C2 and CrAT was downregulated (p< 0.05) in LC2, in relation to FE. In conclusion, although the short-term LC supplementation at 3.72 mM during cryopreservation did not improve post-warming survival and morphological parameters of the evaluated embryos, it was able to modulate expression of genes related to energy homeostasis (CrAT) and oxidative stress (PRDX1), proving to be beneficial, in both forms of supplementation, to in vivo-produced ovine embryos.". 650 $aCarnitine 650 $aCell culture 650 $aCryopreservation 650 $aEwes 650 $aGene expression 650 $aIn vitro culture 650 $aSheep 650 $aVitrification 653 $aAnimal embryos 653 $aSanta Inês 653 $aSurvival 700 1 $aBATISTA, R. I. T. P. 700 1 $aALFRADIQUE, V. A. P. 700 1 $aPINTO, P. H. N. 700 1 $aRIBEIRO, L. S. 700 1 $aOLIVEIRA, C. S. 700 1 $aSOUZA FABJAN, J. M. G. de 700 1 $aCAMARGO, L. S. de A. 700 1 $aFONSECA, J. F. da 700 1 $aBRANDÃO, F. Z.
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