Data/hora: 21/06/2024 18:59:48

Biblioteca(s): 	Embrapa Gado de Leite.	
Data corrente: 	26/12/2018	
Data da última atualização: 	24/01/2023	
Autoria: 	OTERO, R.; HERNÁNDEZ, D.; CAMARGO, L. S. de A.	
Afiliação: 	LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL.	
Título: 	Effect of Trichostatin-A on embryos of bovine clones modified genetically with GFP.	
Ano de publicação: 	2018	
Fonte/Imprenta: 	Indian Journal of Science and Technology, v. 11, n. 25, 2018.	
Páginas: 	9 p.	
DOI: 	10.17485/ijst%2F2018%2Fv11i25%2F128251	
Idioma: 	Inglês	
Conteúdo: 	Abstract Objective: To evaluate the effect of treatment with trichostatin-A (TSA) on the production of bovine embryos, expressing the gene of the green fluorescent protein (GFP) generated by SCNT. Materials: 164 oocytes were distributed in three treatments, NT-GFP: newly reconstructed zygotes with genetically modified cells and not subject to TSA. NT-Trico-GFP: newly reconstructed zygotes with genetically modified cells and subjected to TSA. PART: Zygotes generated by parthenogenetic activation, used as a control for the process of oocyte activation and culture of embryos. The rates of cleavage, blastocysts, and embryos that expressed GFP were assessed by contingency tables and chi-square tests. Results: The percentage of cleavage in the zygotes in the NT-GFP treatment was greater but did not vary significantly from the NT-Trico-GFP treatment. However, this last treatment had a higher percentage of blastocyst formation (p = 0.077). The percentage of blastocysts from cleaved zygotes, the produced embryos were significantly higher (p < 0.05) for the NT-Trico-GFP treatment than for the NT-GFP. In both treatments, all the blastocysts generated expressed the GFP protein. Conclusions:TSA improves the embryonic development of clones of genetically modified cattle that express GFP.	
Palavras-Chave: 	Embryonic Development; Epigenetic Modification; Nuclear Transfer.	
Categoria do assunto: 	X Pesquisa, Tecnologia e Engenharia	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/189328/1/Artigo-Indian-J-Sci-and-Tech-Camargo-Effect.pdf	
Registro original: 	Embrapa Gado de Leite (CNPGL)	
Exemplares: 	CNPGL - (24404-1) - UPC - AP - DD -  -  -  - DIS