Data/hora: 12/06/2024 09:38:37

Biblioteca(s): | Embrapa Gado de Leite.
Data corrente: | 15/01/2018
Data da última atualização: | 12/05/2023
Autoria: | MUNK, M.; ZANETTE, R. de S. S.; CAMARGO, L. S. de A.; SOUZA, N. L. G. D. de; ALMEIDA, C. G. de; GERN, J. C.; GUIMARAES, A. S.; LADEIRA, L. O.; OLIVEIRA, L. F. C. de; BRANDAO, H. de M.
Afiliação: | Michele Munk, UFJF; Rafaella de Souza Salomão Zanette, UFJF; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; Nelson Luís Gonçalves Dias de Souza, Universidade Federal de Tocantins; Camila Guimaraes de Almeida; JULIANA CARINE GERN, CNPGL; ALESSANDRO DE SA GUIMARAES, CNPGL; Luiz Orlando Ladeira, UFMG; Luiz Fernando Cappa de Oliveira, UFJF; HUMBERTO DE MELLO BRANDAO, CNPGL.
Título: | Using carbon nanotubes to deliver genes to hard-to-transfect mammalian primary fibroblast cells.
Ano de publicação: | 2017
Fonte/Imprenta: | Biomedical Physics & Engineering Express, v. 3, n. 4, 045002, 2017.
Idioma: | Inglês
Conteúdo: | The insertion of DNA into primary mammalian cells is an important step towards gene therapy and production of transgenic animals. Recently, carbon nanotubes have been explored as an efficient novel non-viral system for delivering genes to cells due to their unique structure and properties. However, their potential for transfecting primary bovine cells in non-toxic concentrations has not been tested. The purpose of this study was to evaluate the cytotoxicity of carboxylic acid-functionalized multiwalled carbon nanotubes (COOH-MWCNTs) and their use to deliver plasmid DNA encoding the gene of green fluorescent protein to bovine primary fibroblast cells. Flow-cytometry cell viability results have shown the non-toxic nature of COOH-MWCNTs at low concentrations. The frequency shifts in Raman spectroscopy showed that the plasmid DNA connects to the nanomaterial. Fluorescence imaging, flow-cytometry and PCR analysis confirmed that the COOH-MWCNT nanovectors delivered pDNA into primary fibroblast cells successfully. The results show that COOH-MWCNTs can be attractive alternatives for delivery of DNA into hard-to-transfect primary bovine cells.
Palavras-Chave: | COOH-MWCNTs; Delivery of DNA.
Thesaurus Nal: | cytotoxicity; genetic improvement.
Categoria do assunto: | G Melhoramento Genético
Registro original: | Embrapa Gado de Leite (CNPGL)
Exemplares: | CNPGL - (23863-1) - UPC - AP - DD -  -  -  - DIS